Background. Higher positive end-expiratory pressure (PEEP) during laparoscopic surgery may increase oxygenation and respiratory compliance. This meta-analysis aimed to compare the impact of different intraoperative PEEP strategies on arterial oxygenation, compliance, and hemodynamics during laparoscopic surgery in non-obese patients. Methods. We searched RCTs in PubMed, Cochrane Library, Web of Science, and Google Scholar from January 2012 to January 2022 comparing the different intraoperative PEEP (Low PEEP (LPEEP): 0 mbar; Moderate PEEP (MPEEP): 5–8 mbar; high PEEP (HPEEP): >8 mbar; individualized PEEP - iPEEP) on arterial oxygenation, respiratory compliance (Cdyn), mean arterial pressure (MAP), and heart rate (HR). We calculated mean differences (MD) with 95% confidence intervals (CI), and predictive intervals (PI) using random-effects models. The Cochrane Bias Risk Assessment Tool was applied. Results. 21 RCTs (n = 1554) met the inclusion criteria. HPEEP vs LPEEP increased PaO2 (+ 29.38 [16.20; 42.56] mmHg, p 2/FiO2 (+ 36.7 [+ 2.23; +71.70] mmHg, p = 0.04). HPEEP vs MPEEP increased PaO2 (+ 22.00 [+ 1.11; +42.88] mmHg, p = 0.04) or PaO2/FiO2 (+ 42.7 [+ 2.74; +82.67] mmHg, p = 0.04). iPEEP vs MPEEP increased PaO2/FiO2 (+ 115.2 [+ 87.21; +143.20] mmHg, p 2 or PaO2/FiO2 significantly with different heterogeneity. HPEEP vs LPEEP increased Cdyn (+ 7.87 [+ 1.49; +14.25] ml/mbar, p = 0.02). MPEEP vs LPEEP, and HPEEP vs MPEEP didn’t impact Cdyn (p = 0.14 and 0.38, respectively). iPEEP vs LPEEP decreased driving pressure (-4.13 [-2.63; -5.63] mbar, p