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The hemp was principally produced in the west of France, in Normandy, because the weather is adapted. The hemp crop was not demanding, the quantities of water, fertilizer, herbicide were weak. So, other French regions was interested in the production of hemp, like the east of France (Alsace). In this way, in 2020, the Santhica 27 hemp cultivar was carried out in two different localizations in the east of France. In Aspach-le-Bas, the soil was poor and chemical fertilizers were applied. Whereas the soil at Ammertzwiller was rich and healthy and natural fertilizers were used. The soil pH was around 6 for Aspach-le-Bas and 8 for Ammertzwiller. The sowing was in May with a rate of 50 kg/ha. The soil and the stems were harvested at the stem development stage (July), at the flowering stage (August), and at the seed maturity stage (September). SADEF laboratory, specializing in plant nutrition and analysis, performed the chemical composition of the soil and the plant. Moreover, for determining the fiber quality, the stems were manually defibrated, then fibre bundles were combed to get individual fibres. The fiber was put on two self-adhesive stickers with 20-mm-gap. LPMT laboratory, specializing in fiber properties analysis, measured the fiber fineness with the vibroscopic method with VIBROMAT Me. The tensile test was performed with the FAVIMAT instrument. In Aspach-le-Bas, the stems had difficulties to grow up contrary to in Ammertzwiller. The stem height increased significantly between July and August whatever the localization. The stem height and diameter of a healthy and living soil was higher. So, poor soil delayed the hemp development. The proportion of exchangeable elements increased slowly during the cultivation. Phosphorus, potassium, and manganese were in greater quantity in Ammertzwiller. The chemical composition of stem evolved during the cultivation. The rates of iron, calcium, potassium, and nitrogen decreased. The mechanical properties did not evolve during the cultivation, whatever the localization. Overall, the fiber qualities were better in the poor soil. Thus, hemp fibre can be produced for all soil types in the east of France. [ABSTRACT FROM AUTHOR]