Search

The present investigation was carried out in the F3 population of three black gram crosses viz., LBG-787 x LBG-752, TU-94-02 x KU-1006, and TU-94-02 x LBG-752 along with their parents. High PCV and GCV were recorded for plant height, the number of clusters per plant, the number of pods per plant and seed yield per plant in two crosses, LBG-787 x LBG-752 and TU-94-02 x KU-1006 indicating the existence of wide variability for these traits in the progenies of these crosses. High heritability coupled with high genetic advance as per cent of mean were recorded for plant height, the number of clusters per plant, the number of pods per cluster, the number of pods per plant and seed yield per plant in the cross LBG-787 x LBG-752 and for plant height, the number of branches per plant, the number of clusters per plant, the number of pods per cluster, the number of pods per plant and seed yield per plant in the cross TU-94-02 x KU-1006, respectively. This indicates the scope of selection for these traits in a particular population since there is a wide range of variation and additive gene action for the traits. [ABSTRACT FROM AUTHOR]

Objectives: Morphologic detection of connective tissue fiber changes in oral squamous cell carcinoma (OSCC) using special stains remains less documented. The aims of the present study were to study the collagen and elastic fibers in different stages of OSCC and to correlate these changes with two grading systems - Broder's and Bryne's. Study Design: Forty-eight cases of OSCC were studied using hematoxylin and eosin, Verhoeff's - Van Gieson stain for elastic fibers and picrosirius red stain for collagen fibers. The changes were compared with all the grades of carcinoma. Normal mucosa was taken as control. Results: Statistical analysis using Chi-square and ANOVA, showed significant association between the grades of carcinoma and extracellular matrix changes. Greenish-yellow collagen fibers were found to be significantly increased in the poorly differentiated/Grade 3 cases (P< 0.0001) where as well-differentiated/Grade 1 cases showed predominantly reddish-orange and yellowish-orange birefringence of collagen fibers. Chi-square analysis showed a significant amount of fragmented pattern of elastic fibers in poorly differentiated OSCC (χ2 = 104.45, P = 0.009)/Grade 3 OSCC (χ2 = 94.81, P = 0.016). Conclusion: The study of the connective tissue stromal changes can be used as an adjunct to histological grading and thereby helping the surgeon to determine the amount of marginal clearance.

Introduction: Though we are in 21st century with nano technology & tissue printing, there still exist many lacunae in the field of diagnosis. Not much is known about prognostic markers till now from literature to assess potentially malignant lesions. Lesions so called potentially malignant can be termed only after clinical & malignant changes have been developed and there are no means of predicting with certainty the risk of cancerous transformation. Aim: Our present study was undertaken to establish the morphometric parameters of the parabasal and spinous cells of normal oral epithelium with the changes occurring in cells of Oral Leukoplakia (OL), Oral Verrucous Carcinoma (OVC) and Oral Squamous Cell Carcinoma (OSCC). Materials and Methods: Total 40 patients were divided into Group I which includes patients with normal oral mucosa, group II oral leukoplakia patients, group III oral verrucous carcinoma patients and group IV includes oral squamous cell carcinoma patients. Tissue sections were taken and morphometric analysis of cell area, cell diameter, nuclear area, nuclear diameter, nuclear cytoplasmic ratio was done for parabasal and spinous layer cells. Statistical analysis was done using one-way ANOVA and T-test. Results: Nuclear diameter, nuclear area, cell area, nuclear cytoplasmic ratio were significantly increased in OL, OVC, OSCC patients than normal oral mucosa, which was statistically significant. Cell diameter was decreased in OL, OVC, OSCC patients than with normal oral mucosa which was statistically significant. Conclusion: Cellular & nuclear parameters showed statistically significant changes in oral leukoplakia, oral verrucous carcinoma & oral squamous cell carcinoma in comparison with normal oral mucosa.

Oral cancer is becoming one of the most common types of cancer worldwide, particularly in developing countries where this form of malignancy ranks as the 7th and 9th most common cancers in males and females, respectively. There are some challenges in the existing conventional techniques for oral cancer diagnosis. Confocal laser endomicroscopy (CLE) is a novel, non-invasive technique used to obtain micro-anatomical images of the inner lining of hollow organs. The laser confocal endomicroscopy system consists of a low-powered laser (an argon-ion laser that generates an excitation wavelength of 488 nm) that is focused onto a single point in a defined microscopic field of view and the same lens is used as both the condenser and objective folding optical path. The point of illumination thus coincides with the point of detection within the specimen. Light emanating from that point is focused through a pinhole to a detector and light emanating from outside the illuminated spot is rejected. The illumination and detection systems are in the same focal plane and are termed "confocal." CLE is based upon the principle of illuminating a tissue with low power laser and then detecting fluorescent light reflected from the tissue. This permits high-resolution assessment of mucosal histology at cellular and subcellular level. It has been used in a variety of clinical specialties to aid in the diagnosis and treatment planning of inflammatory and neoplastic processes. Intravenous fluorescein dyes are used to highlight the vasculature, lamina propria, intercellular spaces and nuclear staining attained with topical contrast agents like acriflavine and cresyl violet. [ABSTRACT FROM AUTHOR]

About 60-80% of thyroid cancer (TC) cases are papillary thyroid cancer (PTC). Studies have shown that serum adiponectin levels are inversely related to the risk of TC and PTC. Aim of the present study was to evaluate the association between adiponectin rs2241766 and rs266729 polymorphisms and risk of PTC. 122 PTC patients and 128 healthy subjects were enrolled in the study. PCR-RFLP and ARMS-PCR methods were used for genotype analysis. The rs266729 polymorphism did not correlate with risk of PTC. As regard rs2241766 polymorphism, the frequency of the GG genotype did not have a significant difference between the two groups, although, PTC cases showed higher frequency of GT genotype compared to controls (OR=2.87, 95% CI=1.56-5.28, P=0.001). We observed a significant association between adiponectin rs2241766 polymorphism and PTC, however, our result showed no significant relationship between adiponectin rs266729 polymorphism and risk of PTC., Competing Interests: We declare that we have no conflict of interest.