Search

The Cosmology Large Angular Scale Surveyor (CLASS) is a telescope array that observes the cosmic microwave background (CMB) over ∼75% of the sky from the Atacama Desert, Chile, at frequency bands centered near 40, 90, 150, and 220 GHz. CLASS measures the large angular scale CMB polarization to constrain the tensor-to-scalar ratio and the optical depth to last scattering. This paper presents the optical characterization of the 90 GHz telescope. Observations of the Moon establish the pointing while dedicated observations of Jupiter are used for beam calibration. The standard deviations of the pointing error in azimuth, elevation, and boresight angle are 1.′3, 2.′1, and 2.′0, respectively, over the first 3 yr of observations. This corresponds to a pointing uncertainty ∼7% of the beam’s full width at half-maximum (FWHM). The effective azimuthally symmetrized instrument 1D beam estimated at 90 GHz has an FWHM of 0.°620 ± 0.°003 and a solid angle of 138.7 ± 0.6(stats.) ± 1.1(sys.) μ sr integrated to a radius of 4°. The corresponding beam window function drops to ${b}_{{\ell }}^{2}=0.93,0.71,0.14$ at ℓ = 30, 100, 300, respectively. Far-sidelobes are studied using detector-centered intensity maps of the Moon and measured to be at a level of 10 ^−3 or below relative to the peak. The polarization angle of Tau A estimated from preliminary survey maps is 149°.6 ± 0°.2(stats.) in equatorial coordinates. The instrumental temperature-to-polarization ( T → P ) leakage fraction, inferred from per-detector demodulated Jupiter scan data, has a monopole component at the level of 1.7 × 10 ^−3 , a dipole component with an amplitude of 4.3 × 10 ^−3 , with no evidence of quadrupolar leakage.

Abstract Aedes (Ae.) aegypti and Ae. albopictus mosquitoes transmit arthropod-borne diseases around the globe, causing ~ 700,000 deaths each year. Genetic mutants are valuable tools to interrogate both fundamental vector biology and mosquito host factors important for viral infection. However, very few genetic mutants have been described in mosquitoes in comparison to model organisms. The relative ease of applying CRISPR/Cas9-based gene editing has transformed genome engineering and has rapidly increased the number of available gene mutants in mosquitoes. Yet, in vivo studies may not be practical for screening large sets of mutants or possible for laboratories that lack insectaries. Thus, it would be useful to adapt CRISPR/Cas9 systems to common mosquito cell lines. In this study, we generated and characterized a mosquito optimized, plasmid-based CRISPR/Cas9 system for use in U4.4 (Ae. albopictus) and Aag2 (Ae. aegypti) cell lines. We demonstrated highly efficient editing of the AGO1 locus and isolated U4.4 and Aag2 cell lines with reduced AGO1 expression. Further, we used homology-directed repair to establish knock-in Aag2 cell lines with a 3xFLAG-tag at the N-terminus of endogenous AGO1. These experimentally verified plasmids are versatile, cost-effective, and efficiently edit immune competent mosquito cell lines that are widely used in arbovirus studies.

The Cosmology Large Angular Scale Surveyor (CLASS) is a telescope array that observes the cosmic microwave background over 75% of the sky from the Atacama Desert, Chile, at frequency bands centered near 40, 90, 150, and 220 GHz. This paper describes the CLASS data pipeline and maps for 40 GHz observations conducted from 2016 August to 2022 May. We demonstrate how well the CLASS survey strategy, with rapid (∼10 Hz) front-end modulation, recovers the large-scale Galactic polarization signal from the ground: the mapping transfer function recovers ∼67% (85%) of EE and BB ( VV ) power at ℓ = 20 and ∼35% (47%) at ℓ = 10. We present linear and circular polarization maps over 75% of the sky. Simulations based on the data imply the maps have a white noise level of $110\,\mu {\rm{K}}\,\mathrm{arcmin}$ and correlated noise component rising at low- ℓ as ℓ ^−2.4 . The transfer-function-corrected low- ℓ component is comparable to the white noise at the angular knee frequencies of ℓ ≈ 18 (linear polarization) and ℓ ≈ 12 (circular polarization). Finally, we present simulations of the level at which expected sources of systematic error bias the measurements, finding subpercent bias for the Λ cold dark matter EE power spectra. Bias from E -to- B leakage due to the data reduction pipeline and polarization angle uncertainty approaches the expected level for an r = 0.01 BB power spectrum. Improvements to the instrument calibration and the data pipeline will decrease this bias.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.