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To meet the new international efficiency (IE) standards, line-start (LS) synchronous reluctance motors (SyRMs) are now being considered. Conventional star and delta windings are usually employed in LS SyRMs. However, combined star-delta winding has higher winding factor and lower magneto-motive force (mmf) harmonics compared to conventional star and delta windings. Hence, in this paper, LS SyRM and LS ferrite assisted SyRM with star and star-delta winding are analyzed and their performance indices are compared. A winding function analysis is used for the design of star and star-delta windings. Further, the effect of zero sequence current on the performance of the motors under comparison is presented in detail. Star and star-delta winding prototype LS SyRMs with and without ferrite magnets in the rotor are fabricated. Experimental studies are carried-out on these prototypes and the results are presented which are in close agreement with the simulation results. [ABSTRACT FROM AUTHOR]

Electric motors used for irrigation applications contribute to a major part of worldwide energy demand. Efficiency improvement of motors used in these applications is the need of the hour. Usually, line-start (LS) induction motors are used in these applications. However, they are relatively inefficient compared to permanent magnet (PM) motors. On the other hand, PM motors have high initial cost compared to induction motors. In view of this, magnet-less machines like synchronous reluctance motors (SyRM) and PM assisted SyRM are being preferred. SyRM has poor power factor compared to induction motor and hence PM assisted SyRMs are becoming popular. In this article, a 2-pole multi-barrier LS ferrite assisted SyRM is proposed for a bore-well submersible water pump. Two-pole rotor geometry is not extensively studied for LS SyRMs. Further, the issues of ferrite magnet demagnetization for 2-pole LS applications are not explored. A separate cage is avoided by utilizing the rotor flux barriers as a cage, thereby reducing the number of ribs while housing the magnets. This improves the power factor and efficiency of proposed motor. Analytical and finite element analysis (FEA) are used for design of the proposed motor. Further, a prototype is fabricated and experimental results are presented. [ABSTRACT FROM AUTHOR]

Photovoltaic (PV) powered submersible electric water pumps are becoming popular in remote rural areas, due to the nonavailability of grid connectivity. However, the initial cost of a PV system is high. Permanent magnet (PM) brushless dc (BLDC) motors are efficient compared to existing induction motors, which reduce the cost of PV array. However, steep increase in the cost of rare-earth magnets like NdFeB and SmCo makes the use of PM motor uneconomical for PV systems. Thus, the design of PM motors with ferrite magnets is gaining interest. These magnets have low remanence flux density and are brittle. Thus, flux concentration rotor topologies are desirable. The conventional spoke-type (ST) rotor is one such topology. However, flux leakage is one of its prime concerns. To negotiate this, a novel “semi-modular dual-stack” ST BLDC motor along with its parametric aided three-dimensional finite element method analysis is proposed for a PV-based 100 mm deep bore-well submersible water pump. The motor features minimum flux leakage irrespective of rotor bridge width. In addition, a lumped parameter thermal network is modeled for quick estimation of the winding temperature rise. A prototype motor is fabricated to ascertain the results obtained from simulations, and the experimental results are presented. [ABSTRACT FROM PUBLISHER]

Cross magnetization (CM) is an inevitable phenomenon in electrical machines. It is more prominent in interior permanent magnet (IPM) brushless dc (BLDC) machines. This is because PMs demagnetize in overloaded and saturated conditions. It leads to cross coupling between the direct and quadrature-axes quantities, and a decrease in the resultant air-gap flux-density distribution under saturated conditions. In this paper, it is shown that CM leads to braking torque even in unsaturated IPM BLDC machines. Furthermore, the effect of CM on flux linkage, electromotive force, and dynamic torque is presented. Its mitigation using suitable selection of slot–pole combination is presented. It is observed that odd slot–pole combinations are least affected, and even slot–pole combinations are severely affected by CM. Furthermore, it is found that the overlap area of rotor pole arc with the stator pole arc influences the severity of CM, more particularly in fractional slot–pole combination machines. To verify these claims, finite-element method is used for modeling and analyzing various slot–pole combinations of a spoke-type IPM BLDC motor. The results obtained from these simulations are validated with a fabricated reference prototype, which support the claims. [ABSTRACT FROM AUTHOR]

Connective tissue growth factor (CCN2) is a multifunctional matricellular protein, which is frequently overexpressed during organ fibrosis. CCN2 is a mediator of the pro-fibrotic effects of TGF-&bgr; in cultured cells, but the specific function of CCN2 in the fibrotic process has not been elucidated. In this study we characterized the CCN2-dependent signaling pathways that are required for the TGF-&bgr; induced fibrogenic response. By depleting endogenous CCN2 we show that CCN2 is indispensable for the TGF-&bgr;-induced phosphorylation of Smad1 and Erk1/2, but it is unnecessary for the activation of Smad3. TGF-&bgr; stimulation triggered formation of the CCN2/&bgr;3 integrin protein complexes and activation of Src signaling. Furthermore, we demonstrated that signaling through the &agr;v&bgr;3 integrin receptor and Src was required for the TGF-&bgr; induced Smad1 phosphorylation. Recombinant CCN2 activated Src and Erk1/2 signaling, and induced phosphorylation of Fli1, but was unable to stimulate Smad1 or Smad3 phosphorylation. Additional experiments were performed to investigate the role of CCN2 in collagen production. Consistent with the previous studies, blockade of CCN2 abrogated TGF-&bgr;-induced collagen mRNA and protein levels. Recombinant CCN2 potently stimulated collagen mRNA levels and upregulated activity of the COL1A2 promoter, however CCN2 was a weak inducer of collagen protein levels. CCN2 stimulation of collagen was dose-dependent with the lower doses (<50 ng/ml) having a stimulatory effect and higher doses having an inhibitory effect on collagen gene expression. In conclusion, our study defines a novel CCN2/&agr;v&bgr;3 integrin/Src/Smad1 axis that contributes to the pro-fibrotic TGF-&bgr; signaling and suggests that blockade of this pathway may be beneficial for the treatment of fibrosis. [ABSTRACT FROM AUTHOR]

Please cite this paper as: Markiewicz, Nakerakanti, Kapanadze, Ghatnekar and Trojanowska (2011). Connective Tissue Growth Factor (CTGF/CCN2) Mediates Angiogenic Effect of S1P in Human Dermal Microvascular Endothelial Cells. Microcirculation 18(1), 1-11. Abstract Objective: The primary objective of this study was to examine the potential interaction between S1P, a pleiotropic lipid mediator, and CTGF/CCN2, a secreted multimodular protein, in the process of endothelial cell migration. The secondary objective was to determine whether C- and N-terminal domains of CTGF/CCN2 have a specific function in cell migration. Materials and Methods: Migration of HDMECs was examined in monolayer wound healing 'scratch' assay, whereas capillary-like tube formation was examined in three-dimensional collagen co-culture assays. Results: We observed that S1P stimulates migration of HDMECs concomitant with upregulation of CTGF/CCN2 expression. Furthermore, the blockade of endogenous CTGF/CCN2 via siRNA abrogated S1P-induced HDMEC migration and capillary-like tube formation. Full-length CTGF induced cell migration and capillary-like tube formation with a potency similar to that of S1P, while C-terminal domain of CTGF was slightly less effective. However, N-terminal domain had only a residual activity in inducing capillary-like tube formation. Conclusions: This study revealed that CTGF/CCN2 is required for the S1P-induced endothelial cell migration, which suggests that CTGF/CCN2 may be an important mediator of S1P-induced physiological and pathological angiogenesis. Moreover, this study shows that the pro-migratory activity of CTGF/CCN2 is located in the C-terminal domain. [ABSTRACT FROM AUTHOR]

Abstract: The complex physicochemical phenomena occurring in the contact line region of an evaporating meniscus are described using a unique combination of high-resolution experimental data and three complementary models. The following were used: (1) high-resolution experimental liquid profile data (thickness, slope, curvature and curvature gradient) to obtain the pressure gradient in the evaporating pentane meniscus in a vertical constrained vapor bubble (VCVB); (2) macroscopic outside surface temperature profile data; (3) a finite element model to obtain the two-dimensional heat conduction profile in the solid substrate wall (macro-model) and the solid–liquid interfacial temperature profile in the evaporating meniscus region; (4) a continuum fluid-dynamics model (micro-model) to obtain the liquid–vapor interfacial temperature, mass flow rate, Marangoni stresses, and evaporative heat flux profiles along the length of the evaporating meniscus; and (5) the Kelvin–Clapeyron model to obtain the vapor temperature profile (liquid–vapor interfacial temperature jump) in the evaporating meniscus region. The retarded dispersion constant and high-resolution thickness, slope, curvature and curvature gradient profiles were obtained from the experimental reflectivity profiles. There was a substantial increase in the measured curvature in the transition region, where the evaporation rate and flux are a maximum. To obtain numerical closure between the three complementary models, the continuum fluid dynamics model (micro-model) required slip at the solid–liquid interface to support the observed high mass flow rates in the evaporating pentane meniscus. Mass flow rates due to Marangoni stresses, capillary pressure and disjoining pressure are compared. Depending on the liquid thickness, Marangoni stresses can either enhance or hinder fluid flow towards the contact line for the evaporating pure pentane meniscus. Due to the high heat removal rate by the evaporating pentane meniscus in the transition region, dips in the vapor, liquid–vapor and solid–liquid interface temperature were obtained. The results demonstrate and describe the sensitivity and complexity of the phase change process in micro-regions. [Copyright &y& Elsevier]

Abstract: The evaporative heat flux distribution in the leading edge region of a moving evaporating thin liquid film of pentane on quartz was obtained by analyzing the measured thickness profile for thicknesses, δ <2μm. The profiles in a constrained vapor bubble were obtained using image analyzing interferometry. Although the evaporating meniscus appeared to be benign (i.e., without additional observed motion beyond creeping), high heat fluxes were obtained. Significantly higher heat fluxes are possible. The interfacial slope, curvature, interfacial shear stress, and liquid pressure profiles were also obtained. Results obtained using a continuum model were consistent with those obtained using a control volume model. The measured pressure field profile of the isothermal extended meniscus agreed with the constant pressure field predicted by the augmented Young–Laplace model. For the non-isothermal case, measured thickness gradients lead to disjoining pressure and curvature gradients for fluid flow and evaporation. The experimental results demonstrate that disjoining pressure at the contact line controls fluid flow within an evaporating completely wetting thin curved film and is, therefore, a useful boundary condition. However, in small interfacial systems, non-idealities can have a dramatic effect. [Copyright &y& Elsevier]

The thickness and curvature profiles in the contact line region of a moving evaporating thin liquid film of pentane on a quartz substrate were measured for the thickness region, δ<2.5 µm. The critical region, δ<0.1 µm, was emphasized. The profiles were obtained using image-analyzing interferometry and an improved data analysis procedure. The precursor adsorbed film, the thickness, the curvature, and interfacial slope (variation of the local "apparent contact angle") profiles were consistent with previous models based on interfacial concepts. Isothermal equilibrium conditions were used to verify the accuracy of the procedures and to evaluate the retarded dispersion constant in situ. The profiles give fundamental insight into the phenomena of phase change, pressure gradient, fluid flow, spreading, shear stress, and the physics of interfacial phenomena in the contact line region. The experimental results demonstrate explicitly, for the first time with microscopic detail, that the disjoining pressure controls fluid flow within an evaporating completely wetting thin curved film. [ABSTRACT FROM AUTHOR]

A vertical constrained vapor bubble, VCVB, made of fused silica was used to study the stability and oscillations of an evaporating wetting film of HFE- 7000® in a corner. The film thickness profile was measured as a function of time and axial position using an advanced form of image analyzing interferometry. The curvature, apparent contact angle, and pressure profiles for the evaporating film were calculated from the measured film thickness profiles. Oscillation of the liquid film was observed and profiles for both the advancing and receding films were obtained. These are the first such detailed profiles obtained for an oscillating meniscus below a thickness of 0.1 μm.The film thickness profiles demonstrated the spreading of the meniscus during advance as well as the presence of a curvature gradient near the contact line region. The maximum curvature decreased for the advancing menisci and increased with time for the receding menisci. An increase in the adsorbed film thickness was associated with the advancing stage and a decrease with the receding stage. Pressure profiles were measured as a function of position indicating the potential for driving the flow of the fluid toward or away from the contact line. As the film advances or recedes, the pressure gradients change as a function of position fueling the next oscillation cycle. [Copyright &y& Elsevier]

Image analyzing interferometry was used to study the spreading characteristics of an evaporating octane meniscus (purity: 99+%) on a quartz surface. The thickness, slope, and curvature profiles in the contact line region of the meniscus were obtained using a microscopic data analysis procedure. The results obtained for the octane were compared to that of pure pentane (purity: >99.8%) under similar operating conditions. Isothermal experimental conditions of the menisci were used for the in situ estimation of the retarded dispersion constant. The experimental results for the pure pentane demonstrate that the disjoining pressure (the intermolecular interactions) in the thin-film region controls the fluid flow. Also, an imbalance between the disjoining pressure in the thin-film region and the capillary pressure in the thicker meniscus region resulted in a creeping evaporating pentane meniscus, which spreads over the solid (quartz) surface. On the contrary, for less pure octane, the intermolecular interactions between octane and quartz had a significantly different contribution for fluid flow, and hence, the octane meniscus of lower purity did not creep over the quartz surface. As a result, we had a stationary, evaporating octane meniscus. Using the experimental data and a simple model for the velocity distribution, we evaluated the Marangoni shear in a portion of the stationary, evaporating octane meniscus. An extremely small change in the concentration due to distillation had a significant effect on fluid flow and microscale heat transfer. Also, it was found that nonidealities in small interfacial systems, i.e., the presence of impurities in the working fluid, can have a significant effect on the thickness of the adsorbed film, the heat flux, the spreading characteristics of an almost pure fluid, and, therefore, the assumptions in modeling. [ABSTRACT FROM AUTHOR]

Using image-analyzing interferometry, the thickness profile, in the range of δ0 (adsorbed thickness) < δ <3 µm, at the leading edge of a moving ultrathin film with phase change, was measured for a mixture of pentane-octane and compared to that of pure pentane. An improved data-analysis procedure was used to enhance the use of the measured thickness profile. There were significant differences between these two systems, demonstrating the presence of large Marangoni interfacial shear stresses with the mixture. A control volume model was developed to evaluate the differences between the pure fluid and the mixture. The disjoining pressure at the leading edge was found to control fluid flow in the evaporating pure system. However, due to Marangoni stresses, the effect of disjoining pressure on the mixture was found to be small at steady state for the fluxes studied. With an upstream bulk mixture of 2% octane and 98% pentane, a shear stress due to the gradient of the liquid-vapor interfacial surface tension resulting from distillation controlled fluid flow in the contact line region. The average curvature of the evaporating pseudo-steady state pure system was significantly larger (smaller length and larger apparent contact angle at δ=0.1 µm) than the isothermal value, whereas the reverse occurred for the mixture. Using a continuum model, a comparison of numerically obtained Marangoni stresses and local evaporative heat flux profiles between the two systems was also made. [ABSTRACT FROM AUTHOR]

Estrogen is an important regulator of dermal fibroblast functions, including extracellular matrix (ECM) synthesis. Estrogen mediates its effects through estrogen receptors (ERs), ERα and ERβ; however, regulation of ERs in dermal fibroblasts remains poorly understood. Friend leukemia integration factor 1 (Fli1), a member of the Ets transcription factor family, has been shown to play a pivotal role in regulation of the ECM genes in dermal fibroblasts. The aim of this study was to examine a possible interaction between Fli1 and estrogen pathways, focusing on ERα. We show that treatment of human dermal fibroblasts with transforming growth factor-β (TGF-β) increases ERα protein and mRNA levels. Similarly, ERα expression was increased in response to small interfering RNA (siRNA)-mediated depletion of Fli1, suggesting that Fli1 is a mediator of the TGF-β effects on ERα expression. Accordingly, we showed that Fli1 binds to the most proximal region of the ERα promoter, and dissociates from the promoter upon TGF-β treatment. An inverse correlation between Fli1 and ERα expression levels was confirmed in cultured skin fibroblasts obtained from Fli1+/- mice and in the skin of Fli1+/- mice in vivo. This study supports a role of Fli1 as a negative regulator of the ERα gene in dermal fibroblasts. [ABSTRACT FROM AUTHOR]

Background: During scleroderma (SSc) pathogenesis, fibroblasts acquire an activated phenotype characterized by enhanced production of extracellular matrix (ECM) and constitutive activation of several major signaling pathways including extracellular signal-related kinase (ERK1/2). Several studies have addressed the role of ERK1/2 in SSc fibrosis however the mechanism of its prolonged activation in SSc fibroblasts is still unknown. Protein phosphatase 2A (PP2A) is a key serine threonine phosphatase responsible for dephosphorylation of a wide array of signaling molecules. Recently published microarray data from cultured SSc fibroblasts suggests that the catalytic subunit (C-subunit) of PP2A is downregulated in SSc. In this study we examined the role and regulation of PP2A in SSc fibroblasts in the context of ERK1/2 phosphorylation and matrix production. Results: We show for the first time that PP2A mRNA and protein expression are significantly reduced in SSc fibroblasts and correlate with an increase in ERK1/2 phosphorylation and collagen expression. Furthermore, transforming growth factor β (TGFb), a major profibrotic cytokine implicated in SSc fibrosis, downregulates PP2A expression in healthy fibroblasts. PP2A-specific small interfering RNA (siRNA) was utilized to confirm the role of PP2A in ERK1/2 dephosphorylation in dermal fibroblasts. Accordingly, blockade of autocrine TGFβ signaling in SSc fibroblasts using soluble recombinant TGFβ receptor II (SRII) restored PP2A levels and decreased ERK1/2 phosphorylation and collagen expression. In addition, we observed that inhibition of ERK1/2 in SSc fibroblasts increased PP2A expression suggesting that ERK1/2 phosphorylation also contributes to maintaining low levels of PP2A, leading to an even further amplification of ERK1/2 phosphorylation. Conclusions: Taken together, these studies suggest that decreased PP2A levels in SSc is a result of constitutively activated autocrine TGFβ signaling and could contribute to enhanced phosphorylation of ERK1/2 and matrix production in SSc fibroblasts. [ABSTRACT FROM AUTHOR]

Boiling temperatures at 95 kPa, over the entire composition range are measured for the four binary systems formed by m-xylene with n-butanol, iso -butanol, sec -butanol and tert -butanol. A Swietoslawski-type ebulliometer was used for the measurements. The composition versus temperature measurements are well represented by the Wilson model. [ABSTRACT FROM AUTHOR]