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Bacillus strains have long been recognized for their beneficial interactions with plants, enhancing growth, nutrient uptake, and stress resistance. Understanding their molecular mechanisms and plant-microbe interactions is crucial for harnessing their potential in sustainable agriculture. Here we used ten strains from the 5 Bacillus species namely Bacillus velezensis, Bacillus subtilis, Bacillus atrophaeus, Bacillus altitudinis and Bacillus amylofaciens, which are previously reported for PGPR activity. A comparative analysis of these strains was performed to determine their evolutionary relationships, which revealed that Bacillus velezensis and Bacillus amyloliquefaciens are closely related based on underlying genetic and proteomic similarities. Bacillus altitudinis strain LZP02 was the most distantly related to all the other selected strains. On the other hand, Bacillus atrophaeus strains GQJK17 and CNY01 are shown to be closely related to each other. Mauve alignment was performed to determine the genetic relationships between these strains. The LZP02 strain exhibited several unique inversions harboring important genes, such as betB, ftsW, and rodA, which are important for bacterial survival. Proteomic analysis highlighted important pathways that were conserved across these strains, including xenobiotic biodegradation and metabolism, biosynthesis of polyketides and nonribosomal pathways, and biosynthesis of secondary metabolites, all of which have been shown to be involved in plant growth promotion.

Intense cultivation with narrow row spacing in wheat, a common practice in the Indo-Gangetic plains of South Asia, renders the crop more susceptible to lodging during physiological maturity. This susceptibility, compounded by the use of traditional crop cultivars, has led to a substantial decline in overall crop productivity. In response to these challenges, a two-year field study on the system of wheat intensification (SWI) was conducted. The study involved three different cultivation methods in horizontal plots and four wheat genotypes in vertical plots, organized in a strip plot design. Our results exhibited that adoption of SWI at 20 cm × 20 cm resulted in significantly higher intercellular CO2 concentration (5.9-6.3%), transpiration rate (13.2-15.8%), stomatal conductance (55-59%), net photosynthetic rate (126-160%), and photosynthetically active radiation (PAR) interception (1.6-25.2%) over the existing conventional method (plant geometry 22.5 cm × continuous plant to plant spacing) of wheat cultivation. The lodging resistance capacity of both the lower and upper 3rd nodes was significantly higher in the SWI compared to other cultivation methods. Among different genotypes, HD 2967 demonstrated the highest recorded value for lodging resistance capacity, followed by HD 2851, HD 3086, and HD 2894. In addition, adoption of the SWI at 20 cm × 20 cm enhanced crop grain yield by 36.9-41.6%, and biological yield by 27.5-29.8%. Significantly higher soil dehydrogenase activity (12.06 μg TPF g-1 soil hr-1), arylsulfatase activity (82.8 μg p-nitro phenol g-1 soil hr-1), alkaline phosphatase activity (3.11 n moles ethylene g-1 soil hr-1), total polysaccharides, soil microbial biomass carbon, and soil chlorophyll content were also noted under SWI over conventional method of the production. Further, increased root volumes, surface root density and higher NPK uptake were recorded under SWI at 20×20 cm in comparison to rest of the treatments. Among the tested wheat genotypes, HD-2967 and HD-3086 had demonstrated notable increases in grain and biological yields, as well as improvements in the photosynthetically active radiation (PAR) and chlorophyll content. Therefore, adoption of SWI at 20 cm ×20 cm (square planting) with cultivars HD 2967 might be the best strategy for enhancing crop productivity and resource-use efficiency under the similar wheat growing conditions of India and similar agro-ecotypes of the globe.

Despite having a higher burden of health problems, transgender persons face challenges in accessing healthcare in India. Most studies on healthcare access of transgender persons in India focus only on HIV related care, mental healthcare, gender affirmative services or on the ethno-cultural communities or transgender women. This study fills this gap by focusing on diverse gender identities within the transgender community with a specific focus on experiences in accessing general or routine healthcare services. A qualitative descriptive approach was used in this study. 23 in-depth interviews and 6 focus group discussions were conducted virtually and in-person with a total of 63 transgender persons in different regions of India between May and September 2021. The study used a community-based participatory research approach and was informed by the intersectionality approach. Thematic analysis was conducted to analyze the data. Four key themes emerged: (i) intersectional challenges in accessing healthcare start outside of the health system, continue through cisgender-binary-normative health systems that exclude transgender persons; and at the interface with individuals such as health professionals, support staff and bystanders; (ii) the experiences negatively impact transgender persons at an individual level; (iii) in response, transgender persons navigate these challenges across each of the levels: individual, health system level and from outside of the health system. This is a first of its kind qualitative participatory study focusing on routine healthcare services of transgender persons in India. The findings indicate the need to move conversations on trans-inclusion in healthcare from HIV and gender affirmative services to routine comprehensive healthcare services considering the higher burden of health problems in the community and the impact of poor access on their lives and well-being.

Recent development in optical networking employing wavelength division multiplexing (WDM) fulfills the high bandwidth demand applications. Failure of such networks, leads to enormous data and revenue loss. Protection is one of the key techniques, which is used in designing survivable WDM networks. In this paper we compare dedicated and shared protection strategies employed inWDMmesh networks to protect optical networks failure, particularly fiber failure. Dijkstra’s shortest path algorithm is considered for carrying out simulations. The paper compares the performance of protection schemes, such as, dedicated path protection (DPP), shared path protection (SPP) and shared link protection (SLP) schemes. Capacity utilization, switching time and blocking probability are the parameters considered to measure the performance of the protection schemes. Simulation results show that, SPP is more efficient in terms of capacity utilization over DPP and SLP schemes, whereas, SLP offered better switching time than both DPP and SPP schemes. The average call drop rate is minimum for shared path protection scheme and maximum for shared link protection scheme.

IntroductionTransgender persons in India, who are one of the most vulnerable groups, were particularly impacted by the COVID-19 pandemic. Increased risk of COVID-19, challenges with continuing livelihood, uncertainty, and anxiety around the pandemic over pre-existing social discrimination and exclusion pose risk of a mental health impact as well. To investigate this further, this component of a larger study on experiences in healthcare of transgender persons in India during COVID-19 and looks into the question “How did the COVID-19 pandemic impact the mental health of transgender persons in India”.Methods22 In-depth interviews (IDI) and 6 focus group discussions (FGD) were conducted virtually and in-person with persons self-identifying as transgender or belonging to ethnocultural transgender communities from different parts of India. Community based participatory research approach was used by ensuring representation from the community in the research team and through a series of consultative workshops. Purposive sampling with snowballing was used. The IDIs and FGDs were recorded, transcribed verbatim and analyzed using an inductive thematic analysis.ResultsMental health of transgender persons were affected in the following ways. Firstly, COVID-19, its associated fear and suffering combined with pre-existing inaccessibility of healthcare and reduced access to mental health care affected their mental health. Secondly, unique social support needs of transgender persons were disrupted by pandemic linked restrictions. Thirdly, pre-existing vulnerabilities such as precarious employment and underlying stigma were exacerbated. Finally, gender dysphoria was a key mediating factor in the impact of COVID-19 on mental health with a negative and positive impact.ConclusionsThe study reiterates the need to make systemic changes to make mental healthcare and general healthcare services trans-inclusive while also recognizing the essential nature of gender affirmative services and the need to continue them even during emergencies and disaster situations. While this brings out how public health emergencies can exacerbate vulnerabilities, it also shows how the lived mental health experience of transgender person is intricately linked to the way work, travel and housing is structured in our society and therefore points to the structural nature of the linkage between mental health and gender.

The All India Institute of Medical Sciences (AIIMS), New Delhi published guidelines, titled “Report of the Expert Group for framing Guidelines for admission of candidates with benchmark disabilities in postgraduate courses at AIIMS, New Delhi”, for doctors with disabilities seeking postgraduate admissions to the Institutions of National Importance. The expert group, which lacked representation of people with disabilities, in general, and doctors with disabilities, in particular, puts immense effort into justifying why trainees with disabilities must not join AIIMS, at times in bold and/or capital letters, and at other places, in an outright ableist language. In addition, there is blatant plagiarism from well-known advisories and guidelines which are known for promoting the inclusion of trainees with disabilities. Attitudinal barriers and biases remained incorrigible as sections of these documents were selectively abridged to justify prevailing exclusion practises. We link the connection of these members to the controversial National Medical Council guidelines for undergraduate admission of persons with specified disabilities, which were successfully challenged in courts, as well as to the identification of posts for employment at AIIMS. We justify disability accommodations by citing inclusive court interventions from India to emphasise that the notion of inclusive equality includes the provision for reasonable accommodations. It is high time that the motto “Nothing about us, without us” becomes a locus classicus for immediate change of these discriminatory guidelines, as well as the prescribing powers of such experts.

Parkinson’s disease (PD) is associated with a mutation in the PRKN (Parkin RBR E3 Ubiquitin Protein Ligase). PRKN functions as maintaining dopamine (DA) neuronal homeostasis and homeostasis dysfunction, which play an important role in early PD onset. The main objective behind this work is to identify PRKN deleterious single nucleotide polymorphism (SNPs) by computational analysis and implement molecular dynamic simulation (MDS) to study the structural and functional properties of native and mutant proteins. We found A46T, I23T, C212Y, D243N, E28G, G359D, P37L, Q34R, R256C, R275W, R366W, and R402C as the most delirious and disease-linked by using different bioinformatics tools such as ANNOVAR (SIFT & POLYPHEN2) annotation, SNPs & GO, PROVEAN, and I-Mutant3.0. To understand protein functionality and atomic arrangement in 3-D space, the comparative modeling of native and mutant PRKN protein (A46T, I23T, C212Y, D243N, E28G, G359D, P37L, Q34R, R256C, R275W, R366W, and R402C) was performed with standalone MODELLER 9.15 program. Finally, MDS analysis was done by using the GROMACS 5.1.5 comprehensive package to study the structural stability and dynamic perturbations in native and mutant PRKN proteins. MDS analysis shows more flexibility in native structures in comparison to mutant structures. Mutant structures show structural perturbation and result in loss of PRKN protein function. The findings of this study might assist wet-lab studies to create effective pharmacological treatments against PD targeting PRKN.

In Columbia, Rodinia and Gondwana supercontinental assembly periods, eastern margin of India lay quite close to the east Antarctica. During these periods, this region underwent prolonged sedimentation, magmatism, oceanic subduction, multiple accretion and continent-continent collision which have left their imprints in the geological and geophysical observations. In the present study, we have analyzed crustal seismic velocity structure along the DSS profiles located in the eastern part of the Indian shield that cuts across the Proterozoic Cuddapah Basin, Godavari and Mahanadi Gondwana grabens. The study revealed presence of a prominent high velocity (6.2–6.5 km/s) hidden ridge structure, which is 30 to 50 km wide and located at a shallow subsurface depth beneath the Nellore Schist Belt and Eastern Ghats Belt. It underlies below Kaligiri-Duttaluru along the Kavali-Parnapalle profile, Darsi-Addanki along the Alampur-Koniki-Ganapeswaram profile and Sattupalli-Ashwaraopet in Kallur-Polavaram profile. This ridge structure is conspicuously characterized by high gravity anomaly gradients from -90 to -15 mGal. Gravity patterns over these regions further indicate that this ridge which appears to continue from the north of Chennai to Bhavanipatna region in Bastar Craton, may have been formed due to the collision of East Antarctic terrain with eastern Indian Shield after the cessation of supra-subduction at the end of Mesoproterozoic. Further, this ridge structure is flanked by Proterozoic sediments on either side; in Cuddapah Basin on its western side and East Coast Sedimentary Basin on its eastern side, which together with the possible occurrence of Proterozoic sediments in other basins, would indicate that the extent of Proterozoic sedimentation was much larger than hitherto known. We suggest that this region was rifting during Paleo-Mesoproterozoic period and possibly a shallow marine basin existed, when it was an integral part of the Columbia supercontinent assembly. It underwent another rifting phase and denudation during the Gondwana period as indicated by thin patches of Gondwana sediments which are lined up all along the east coast. Quite likely, the rifting between India and Antarctica during the Gondwana super assembly period initiated in the Permo-Triassic Gondwana period itself, with final separation taking place much later during early Cretaceous period.

The disability viewpoint is fundamental for understanding and advancing social justice for everyone in the population. Despite this fact, it is regularly dismissed by public health experts and policymakers. Understanding of disability rights is central in an all-inclusive COVID-19 preparedness. This paper attempts to explore disability ethics in understanding structural discrimination, equitable practices, respect for disability culture and ways to safeguard health care professionals with disabilities in the coronavirus pandemic. In crisis standards of care, resource allocations must not be solely based on a disabled person's subjective quality of life. Health professionals should avoid stereotypes about an individual's disability to ration care. Triage protocol committees and disaster risk reduction working groups should explicitly recruit people with disabilities and chronic illnesses in their response strategies. Disability ethics can reform medical rationing by removing prejudices and safeguarding fair protection of the interests of all patients, including those with a disability.

The new curriculum of the Medical Council of India (MCI) lacks disability-related competencies. This further involves the risk of perpetuating the medicalization of diverse human experiences and many medical students may graduate with little to no exposure to the principles of disability-inclusive compassionate care. Taking into consideration the UN Convention, the Rights of Persons with Disabilities, Act 2016, and by involving the three key stakeholders – disability rights activists, doctors with disabilities, and health profession educators – in the focus group discussions, 52 disability competencies were framed under the five roles of an Indian Medical Graduate (IMG) as prescribed by the MCI. Based on feedback from other stakeholders all over India, the competencies were further refined into 27 disability competencies (clinician: 9; leader: 4; communicator: 5; lifelong learner: 5; and professional: 4) which the stakeholders felt should be demonstrated by health professionals while they care for patients with disabilities. The competencies are based on the human rights approach to disability and are also aligned with the competencies defined by accreditation boards in the US and in Canada. The paper describes the approach used in the framing of these competencies, and how parts of these were ultimately included in the new competency-based medical education curriculum in India.

Introduction: Faculty development to implement competency-based medical education (CBME) is urgently needed as the Medical Council of India has implemented the competency-based curriculum this year onwards. Objectives: To evaluate a 2-day faculty development workshop in terms of: (a) increase in knowledge about CBME terminology and concepts, (b) self-reported capacity to develop and implement a competency-based module in their respective disciplines, and (c) satisfaction of the participants. Methodology: A single arm interventional study using mixed methods was carried out in which faculty members were purposively identified and requested to volunteer for a two-day faculty development workshop on the development and implementation of CBME. The workshop was evaluated (open-ended and Likert scored items) by the participants for self-reported gain in knowledge, gain in their confidence to develop and implement CBME, and level of satisfaction with respect to the components of the workshop. Quantitative data was analyzed by Wilcoxon signed rank test and Kruskal–Wallis test. Qualitative data was analyzed by doing content analysis and emerging themes have been presented. Results: Eleven faculty members attended a capacity building workshop for developing and implementing a competency-based curriculum for medical undergraduates. There was a significant improvement in their self-reported knowledge and attitude regarding the competency-based curriculum. New concepts learned fell into four domains: modification of the existing curriculum toward competency-based curriculum, knowledge of CBME, teaching-learning and assessment methods, and beneficial to the students. The participants were highly satisfied with the workshop in its current form. Conclusion: A two-day faculty development workshop can increase the knowledge and understanding of competency-based medical education and can be an important first step in the journey to more specialized training.

In the present study twenty five human urine samples of hospital patients were screened for Staphylococcus aureus. Twenty percent of the samples were positive for Staphylococcus aureus as yellow colonies were observed aerobically on mannitol salt agar, out of the five isolates two were catalase positive. On the basis of resistance against methicillin, out of the three catalase positive isolates, two were MSSA and one was MRSA. One MRSA and one MSSA strain was examined using PCR technique, for gene identification. The mecA and nuc gene were amplified using PCR technique. The presence of mecA gene and nuc gene in MRSA and MSSA respectively, confirmed the strains. Comparative gene analysis of mecA gene and nuc gene was performed using BLAST and CLUSTALW tools. Thereafter rooted phylogenetic tree was obtained for both mecA and nuc gene sequences explaining the genetic evolutionary lineage. Present study analyzed the resistance mechanism of MRSA and to identify the gene responsible in order to control the re-emerging methicillin resistant Staphylococcus aureus infection.

Purpose: Ocular trauma constitutes an important cause of preventable visual morbidity worldwide. This study was done to study the incidence, sociodemographic pattern, and clinical profile of ocular trauma in pediatric age group. Also to evaluate the factors influencing final visual outcome in these patients. Methods: This was a prospective interventional study concerning ocular trauma in pediatric patients up to 16 years of age of either sex. Various variables having an impact on final visual outcome were studied, and results were analyzed using statistical indices – relative risk, Chi-square test, P value, and linear regression analysis. Results: A total of 220 cases of trauma were evaluated with the mean age being 8.74 ± 3.93 years, males were predominantly affected and open globe injuries outnumbered blunt injuries. Penetrating injuries accounted for 67.79% cases of open globe injury, rupture being the least (2.54%). Stepwise multiple linear regression analysis executed, showed the best predictors in the descending order for final visual outcome were presenting visual acuity, size of corneal tear, type of injury, zone of injury, time period between injury and treatment with a variance of 35.9%, 6.3%, 5.3%, 3.7%, and 2.7%, respectively. All above variables were also found to be statistically significant (P < 0.05) on Chi-square test. Conclusion: We report the first study on the epidemiology and clinical outcomes of pediatric ocular trauma in central India. Poor initial Visual Acuity and posterior segment involvement adversely affect the visual outcome. Early medical treatment and globe-salvaging repair should be done in all eyes suffering from trauma.

Purpose The purpose of this study was to assess the clinical empathy of a cohort of medical students spanning 4 years of undergraduate study and to identify factors associated with empathy. Methods A cross-sectional study to assess the empathy of undergraduate medical students at the University College of Medical Sciences and GTB Hospital in Delhi, India, was conducted using the Jefferson Scale of Empathy–Student Version. Demographic data were obtained using a pre-tested, semi-open-ended questionnaire. Results Of the 600 students, 418 participated in the survey (69.7%). The mean empathy score was 96.01 (of a maximum of 140), with a standard deviation of 14.56. The empathy scores decreased from the first to the third semester, plateaued at the fifth semester, and rose again in the seventh semester. Empathy was found to be significantly associated with the gender of the participant, with females having higher scores (P

Stakeholders, including patients and employers, find that skills pertaining to professionalism, humanism, diversity, communication, and ethics are as important for patient care as the doctor’s ability to diagnose and treat illness. Practitioners should be able to demonstrate these skills in real time, yet they are not explicitly taught in the medical course – students are expected to learn them through observation of role models. Some students may never witness such role modeling. Research suggests that the creative instincts of medical students could be utilized through exposure to the humanities to explicitly develop these skills. Medical educators worldwide are examining newer ways to actively train and assess learners in professionalism and related competencies.⁠ Using Rudyard Kipling’s “Five Ws and One H” guide to writing a scientific paper,⁠ we propose the ABCDE paradigm and demonstrate why it is most appropriate to use the medical humanities to teach professionalism and humanism.

India has the third largest number of people living with HIV in the world. The UNAIDS Gap report has identified twelve risk groups that are especially vulnerable and have been left behind from the national AIDS response. Of these twelve, one is persons with disabilities. Disability is both a public health issue and a human rights issue; persons with disabilities are the world’s largest minority. Low awareness, sexual abuse, and lack of access to health services are the major reasons for people with disabilities being vulnerable. While the gap report is a landmark report, in that it compartmentalizes the risk groups, disability cannot be looked at in isolation. Since any of the other risk groups may include persons with disabilities, the issue is a complex one meriting greater attention. The National AIDS Control Organization has completely ignored this group of persons. To efficiently close the gap, an integrated and disability-inclusive HIV response is needed so that people with different types of disabilities, their caretakers, healthcare professionals and society are empowered to fight the collective battle against HIV/AIDS.

The Rime of the Ancient Mariner was written by Coleridge and is a classic poetry about retribution, punishment, guilt, and curse. Religious beliefs and delusions can arise from neurologic lesions and anomalous experiences, suggesting that at least some religious beliefs can be pathological. Looking at the poem through the psychiatric and psychological domain, the symbolism, the narration and the entire setting of the poem represents Post Traumatic Stress Disorder. Mariner′s reactions are beautifully portrayed from the psychoanalytic point of view and the literary piece shows claustrophobia, stygiophobia, dikephobia, and poinephobia. The mental stress of a person under a crisis situation has remarkably been evoked in this poem. This incredible piece of art expresses how the realization of divine love within oneself has the power to heal pain and suffer.

Purpose: This study aimed to assess medical students’ presence on Facebook and the extent of their visible activity, with particular reference to online professionalism. Methods: This was a cross-sectional study including all medical students enrolled in the University College of Medical Sciences, University of Delhi, India during the period of the study, which was conducted from 2011 to 2012. After approval by the Institutional Ethical Committee, the full names of all students were obtained from our institution. After creating a fictitious profile, Facebook was searched for students’ profiles, and those found were examined for visible content and unprofessional behaviour. Results: Of 611 students, 477 (78.1%) had detectable Facebook profiles. Out of 477 profiles, date of birth, address, email, phone number, religion, and political views were rarely shared; sexual orientation and relationship status were displayed on approximately one third of the profiles; and an identifiable profile picture (80.3%), field of study (51.6%), and institution (86.2%) were commonly shared . The visible content included friend lists (88.7%), photo albums (36.1%), and associations with diverse groups and pages (97.1%). Five profiles (1.05%) displayed unprofessional content, including one profile photograph depicting alcohol consumption, one association with groups relating to excessive alcohol consumption, two profiles containing sexually explicit language, and one association with a sexist page. Conclusion: Most of our students use Facebook’s privacy settings to hide some content from others. Unprofessional content was rarely visible from a stranger’s profile. However, even when hidden from strangers, unprofessional behaviour is still unprofessional behaviour. As Facebook is an integral part of life, it is important for medical educators and students to understand the implications and importance of e-professionalism. Professionalism curricula should address e-professionalism.

Purpose: The first year is stressful for new medical students who have to cope with curricular challenges, relocation issues, and separation from family. Mentoring reduces stress and facilitates adaptation. A program for faculty mentoring of first-semester students was initiated by the Medical Education Unit in 2009 at University College of Medical Sciences, Delhi. Feedback after the first year revealed that mentees were reluctant to meet their mentors, some of whom were senior faculty. In the following year, student mentors (near-peers) were recruited to see if that would improve the rate and quality of contact between mentees and mentors. Methods: Volunteer faculty (n=52), near-peers (n=57), and new entrants (n=148) admitted in 2010 participated in the ratio of 1:1:3. The program aims were explained through an open house meeting, for reinforcement, and another meeting was conducted 5 months later. At year-end, a feedback questionnaire was administered (response rate: faculty, 28 [54%]; mentees, 74 [50%]). Results: Many respondent faculty (27, 96%) and mentees (65, 88%) believed that near-peer mentoring was useful. Compared to the preceding year, the proportion of meetings between faculty mentors and mentees increased from 4.0±5.2 to 7.4±8.8; mentees who reported benefit increased from 23/78 (33%) to 34/74 (46%). Benefits resulted from mentors’ and near-peers’ demonstration of concern/support/interaction/counseling (35, 47.3% mentees); 23 mentees (82%) wanted to become near-peers themselves. Conclusion: Near-peer mentoring supplements faculty mentoring of first-year medical students by increasing system effectiveness.

Resident-as-teacher courses are pretty common in Western medical schools however they are a rarity in Asian and developing countries. The current report is a scholarly analysis of a three day orientation program for senior residents in order to improve their functioning by providing new template either for supplementing basic workshops for faculty or to advocate a change in system. The experience gained by Medical Education Unit of University College of Medical Sciences can be used to conduct training breeding grounds at national or regional levels. Resident as teachers educational interventions need to be designed taking into account their impact on education system.