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The present study examined the effects of mesoporous silica nanoparticles (MSNs) on its adsorption capacity of aflatoxin B1 (AFB1). Moreover, the study evaluated the toxicity of MSNs with AFB1 using NIH3T3 cells and hemolysis test. The obtained MSNs were spherical, irregular-like in shape, having a mean size of 39.97 ± 7.85 nm and a BET surface area of 1195 m2/g. At 0.1 mg mL−1 concentration of MSN, the AFB1 adsorption capacity was 30%, which reached 70% when the MSN concentration increased to 2.0 mg mL−1. Our findings showed that AFB1 was adsorbed (∼67%) in the first few minutes on being in contact with MSNs, reaching an adsorption capacity of ∼70% after 15 min. Thereafter, the adsorption capacity remained constant in solution, demonstrating that the MSNs adsorbed toxins even beyond overnight. MSN treatment (0.5–2.0 mg mL−1) using NIH3T3 cells did not result in any reduction in cell viability. In addition, MSN treatment completely reversed the cytotoxic effect of AFB1 at all concentrations. Hemolysis test also revealed no hemolysis in MSNs evaluated alone and in those combined with AFB1. To the best of our knowledge, this study is the first to demonstrate that MSN can reduce cell toxicity produced by AFB1 due to its potential to adsorb mycotoxins. [ABSTRACT FROM AUTHOR]

Abstract: The occurrence of aflatoxin M1 (AFM1), ochratoxin A (OTA), and deoxynivalenol (DON) in human milk and their correlation to the lactating donors dietary habits from Southern Brazil were investigated. Milk samples were collected from 86 nursing mothers who were also interviewed through a Food Frequency Questionnaire (FFQ). The occurrence of AFM1, OTA, and DON in the human milk samples was evaluated by liquid chromatography tandem mass spectrometry (LC‐MS/MS) and correlated with the FFQ data. The analysis of the FFQs revealed a high intake of foods susceptible to mycotoxin contamination, such as wheat flour and its derivatives, bovine milk, and coffee which had habitual consumption reported by 71, 50, and 43% of the participants, respectively. AFM1, OTA, and DON were not detected in any sample of human milk, even though the results obtained by LC‐MS/MS suggest a possible occurrence of DON in four samples (>LOD: 2.5 ng/mL;

The present study examined the effects of mesoporous silica nanoparticles (MSNs) on its adsorption capacity of aflatoxin B 1 (AFB 1 ). Moreover, the study evaluated the toxicity of MSNs with AFB 1 using NIH3T3 cells and hemolysis test. The obtained MSNs were spherical, irregular-like in shape, having a mean size of 39.97 ± 7.85 nm and a BET surface area of 1195 m 2 /g. At 0.1 mg mL -1 concentration of MSN, the AFB 1 adsorption capacity was 30%, which reached 70% when the MSN concentration increased to 2.0 mg mL -1 . Our findings showed that AFB 1 was adsorbed (∼67%) in the first few minutes on being in contact with MSNs, reaching an adsorption capacity of ∼70% after 15 min. Thereafter, the adsorption capacity remained constant in solution, demonstrating that the MSNs adsorbed toxins even beyond overnight. MSN treatment (0.5-2.0 mg mL -1 ) using NIH3T3 cells did not result in any reduction in cell viability. In addition, MSN treatment completely reversed the cytotoxic effect of AFB 1 at all concentrations. Hemolysis test also revealed no hemolysis in MSNs evaluated alone and in those combined with AFB 1 . To the best of our knowledge, this study is the first to demonstrate that MSN can reduce cell toxicity produced by AFB 1 due to its potential to adsorb mycotoxins.