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The seedling length testing is a good methodology for seed vigor evaluation. It's low cost, no need of sophisticated equipments, neither human resource very specialized associated with quick results has made it very popular. However, it has been observed presence of seedlings with short and tickly hypcotil, when conduced with 20 soybean seeds, which it may interfere in the results. The objective of this work was to evaluate the effect of sample size (10 and 20 seeds), germination roll diameters (three diameters) and seeds size (sieves 12, 13 and 14) on the results of the soybean seedling length. In this study, tests with 20 seeds per germination roll, with seeds arrangement in two rows, the diameter of the germination roll and the seeds size interfered in the results. However, the test with 10 seeds per germination roll did not affect the results, indicating that this favors the result reproducibility. [ABSTRACT FROM AUTHOR]