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Land snails occupy a variety of habitats, with differing temperature and humidity regimes and exhibit a wide span of adaptations, to withstand abiotic condition changes. The present work's aim was to examine the correlation of habitat's thermal adversity in different Mediterranean type habitats with the land snail's antioxidant and heat shock responses. For this purpose, snails of different species from populations along a north-south axis from the islands and mainland of Greece were exposed to elevated temperature and antioxidant enzyme activities, and Hsp70 and Hsp90 levels were determined in their tissues. The ATP, ADP, and AMP levels and the adenylate energy charge (AEC) were also determined. The comparison of protein levels and enzymatic activities across time intervals revealed significant differences for all factors examined. While the gradation pattern over time for a given factor was similar in all populations the absolute values over time differed. Catalase activity and the Hsp90 protein levels had the higher contribution in separating the different species and populations, followed by the activity of glutathione reductase and Hsp70 protein levels which contributed to a lesser degree. In general, populations from the southern part of their distribution in Greece tend to display a faster increase than northern populations in induction levels of all factors examined. Our data seem to be in line with the concept of preparation for oxidative stress (POS) while the changes in the AEC indicate an early preparation to cover the energy demand for the induction and synthesis of antioxidant enzymes and Hsps., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Background: This retrospective study was conducted to compare the conventional cytospin method and ThinPrep liquid-based urinary cytology in diagnosing bladder cancer using The Paris System (TPS) of classification., Methods: We retrieved files for 2020, at the Cytopathology Department of Laiko Hospital, of urinary cases diagnosed according to TPS. Cytospin and ThinPrep slides were separately reviewed and new diagnoses were rendered, then compared with the original diagnosis and histology when available. Risk of high-grade malignancy (ROHM) for each TPS category was assessed, along with accuracy parameters of each method and their combination., Results: The study material comprised 100 cases of void urinary cytology classified as 20 high-grade urothelial carcinoma (HGUC = TPS5) cases, 20 of suspicion for HGUC (SHGUC = TPS4), 25 of atypical urothelial cells (AUC = TPS3), and 35 of negative for HGUC (NHGUC = TPS2). A single inadequate (TPS1) case and 4 of low-grade urothelial neoplasm (TPS6) were excluded as small in number. The ROHM was 95% for HGUC, 55% for SHGUC, 28% for AUC and 5.7% for NHGUC. Agreement with the original diagnosis was 86% for cytospin and 82% for ThinPrep. No significant differences were observed among the two techniques or their combination regarding sensitivity and specificity, with a mild advantage for cytospin. Interobserver reproducibility and repeatability were high., Conclusion: No significant differences were found concerning sensitivity and specificity between cytospin and ThinPrep when applying TPS criteria. TPS is a reliable classification scheme for either conventional/cytospin or liquid-based cytology, or their combination., (© 2021 John Wiley & Sons Ltd.)

Background: We investigated serous effusions occurring during the course of an already known hematologic neoplasia or as a first manifestation of it. We correlated cytology results with flow cytometry results, when available. In the absence of flow cytometry, our correlation was based on clinical follow up information obtained retrospectively. We evaluated our results in relation to the data of the literature and we considered some new suggestions for the improvement of cytology service., Methods: Serous effusions in hematologic patients were retrieved from the files of the Department of Cytology, Laiko Hospital, for a period of 2 years. All patients had enrolled either a previous hematologic history, or a suspicious clinical and imaging status. Seventy-three serous effusions were included. Cytology reports consisting of morphology and immunocytochemistry assessment were correlated to flow cytometry results and, occasionally, to clinical follow-up., Results: In the group of patients with previous history, sensitivity was 82.76%, positive predictive value was 100%, specificity 100%, and negative predictive value was 58.33%. In the group of patients without previous history, sensitivity and positive predictive value were both 91%, whereas specificity and negative predictive value could not be estimated., Conclusion: We provide evidence that the diagnostic accuracy of cytology with the adjunct of immunocytochemistry is high compared to flow cytometry for detecting hematologic malignancies. In order to improve clinical performance, it is suggested that a cytology triage of serous effusions in all patients with hematologic malignancy must be implemented., (© 2018 Wiley Periodicals, Inc.)