1. FAK regulates tension transmission to the nucleus and endothelial transcriptome independent of kinase activity
- Author
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Md Zahid Akhter, Pascal Yazbeck, Mohammad Tauseef, Mumtaz Anwar, Faruk Hossen, Sayanti Datta, Vigneshwaran Vellingiri, Jagdish Chandra Joshi, Peter T. Toth, Nityanand Srivastava, Stephen Lenzini, Guangjin Zhou, James Lee, Mukesh K. Jain, Jae-Won Shin, and Dolly Mehta
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CP: Cell biology ,Biology (General) ,QH301-705.5 - Abstract
Summary: The mechanical environment generated through the adhesive interaction of endothelial cells (ECs) with the matrix controls nuclear tension, preventing aberrant gene synthesis and the transition from restrictive to leaky endothelium, a hallmark of acute lung injury (ALI). However, the mechanisms controlling tension transmission to the nucleus and EC-restrictive fate remain elusive. Here, we demonstrate that, in a kinase-independent manner, focal adhesion kinase (FAK) safeguards tension transmission to the nucleus to maintain EC-restrictive fate. In FAK-depleted ECs, robust activation of the RhoA-Rho-kinase pathway increased EC tension and phosphorylation of the nuclear envelope protein, emerin, activating DNMT3a. Activated DNMT3a methylates the KLF2 promoter, impairing the synthesis of KLF2 and its target S1PR1 to induce the leaky EC transcriptome. Repleting FAK (wild type or kinase dead) or inhibiting RhoA-emerin-DNMT3a activities in damaged lung ECs restored KLF2 transcription of the restrictive EC transcriptome. Thus, FAK sensing and control of tension transmission to the nucleus govern restrictive endothelium to maintain lung homeostasis.
- Published
- 2024
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