Search

Your search keyword '"Scarsella, Gianfranco"' showing total 103 results
Start Over Author "Scarsella, Gianfranco"
103 results on '"Scarsella, Gianfranco"'

13. Apoptosi e neurobiologia della retina

Author

Limoli, Paolo G., Pescosolido, Nicola, Pascarella, Antonia, and Scarsella, Gianfranco

Subjects
retina, malattie neurodegenerative, Apoptosi
Published
2014

15. Degenerative effects in rat eyes after experimental ocular hypertension

Author

Scarsella, Gianfranco, Nebbioso, Marcella, Stefanini, S., Librando, Aloisa, and Pescosolido, Nicola

Subjects
Retinal degeneration, Male, Pathology, medicine.medical_specialty, Histology, genetic structures, Angiogenesis, Biophysics, Ocular hypertension, glaucoma, vegf immunolocalization, bandeira simplicifolia isolectin b-4 labelling, bandeira simplicifolia isolectin b 4 labelling, bandeira simplicifolia isolectin b4 labelling, glial fibrillary acidic protein immunolocalization, angiogenesis, Eye, Retina, chemistry.chemical_compound, medicine, Animals, Rats, Wistar, Ganglion cell layer, lcsh:QH301-705.5, Plexus, Original Paper, angiogenesis, Bandeira simplicifolia isolectin B4 labelling, Glaucoma, Glial fibrillary acidic protein immunolocalization, VEGF immunolocalization, Glial fibrillary acidic protein, biology, VEGF immunolocalization, Retinal Degeneration, Bandeira simplicifolia isolectin B4 labelling, Glaucoma, Cell Biology, medicine.disease, Immunohistochemistry, eye diseases, Rats, Vascular endothelial growth factor, Disease Models, Animal, medicine.anatomical_structure, chemistry, lcsh:Biology (General), Glial fibrillary acidic protein immunolocalization, biology.protein, Ocular Hypertension, sense organs
Abstract

This study was used to evaluate the degenerative effects on the retina and eye-cup sections after experimental induction of acute ocular hypertension on animal models. In particular, vascular events were directly focused in this research in order to assess the vascular remodeling after transient ocular hypertension on rat models. After local anaesthesia by administration of eye drops of 0.4% oxibuprocaine, 16 male adult Wistar rats were injected in the anterior chamber of the right eye with 15 µL of methylcellulose (MTC) 2% in physiological solution. The morphology and the vessels of the retina and eye-cup sections were examined in animals sacrificed 72 h after induction of ocular hypertension. In retinal fluorescein angiographies (FAGs), by means of fluorescein isothiocyanate-coniugated dextran (FITC), the radial venules showed enlargements and increased branching, while the arterioles appeared focally thickened. The length and size of actually perfused vessels appeared increased in the whole superficial plexus. In eye-cup sections of MTC-injected animals, in deep plexus and connecting layer there was a bigger increase of vessels than in controls. Moreover, the immunolocalization of astrocytic marker glial fibrillary acidic protein (GFAP) revealed its increased expression in internal limiting membrane and ganglion cell layer, as well as its presence in Müller cells. Finally, the pro-angiogenic factor vascular endothelial growth factor (VEGF) was found to be especially expressed by neurones of ganglion cell layer, both in control and in MTC-injected eyes. The data obtained in this experimental model on the interactions among glia, vessels and neurons should be useful to evaluate if also in glaucomatous patients the activation of vessel-adjacent glial cells might play key roles in following neuronal dysfunction.

Published
2012

28. Coinvolgimento del TGF-β nelle patologie della retina

Author

Pescosolido, Nicola, Cantarelli, C., Paffetti, L., and Scarsella, Gianfranco

Subjects
TGF-β, Fattori di crescita, Vitreoretinopatia proliferativa, Vitreoretinopatia diabetica, Cellule endoteliali
Published
2004

33. Il Trasforming Growth Factor-β

Author

Pescosolido, Nicola, Cantarelli, C., Paffetti, L., and Scarsella, Gianfranco

Subjects
TGF- β, Cellule epiteliali, Citochine
Published
2003

34. La comunicazione intercellulare

Author

Pescosolido, Nicola, Cantarelli, C., Paffetti, L., and Scarsella, Gianfranco

Subjects
Comunicazione intercellulare, Recettori, Terapia farmacologica, Fattori di crescita
Published
2003

46. Stability of Drugs of Abuse in Urine Samples at Room Temperature by Use of a Salts Mixture

Author

Pellegrini, Manuela, Graziano, Silvia, Mastrobattista, Luisa, Minutillo, Adele, Busardo, Francesco Paolo, and Scarsella, Gianfranco

Abstract

Background: It has long been recognized that ensuring analyte stability is of crucial importance in the use of any quantitative bioanalytical method. As analyses are usually not performed directly after collection of the biological samples, but after these have been processed and stored, it is essential that analyte stability can be maintained at storage conditions to ensure that the obtained concentration results adequately reflect those directly after sampling. The conservation of urine samples in refrigerated/ frozen conditions is strongly recommended; but not always feasible. The aim of this study was to assess the stability of some well-known drugs of abuse methamphetamine (MA), 11-nor-9-carboxy-Δ9- tetrahydrocannabinol (THC-COOH), benzoylecgonine (BE), and morphine (MOR) in urine samples kept at room temperature by adding a salt mixture (sodium citrate, sodium ascorbate, borax). Methods: Two different urine samples were prepared with and without salt mixture, stored at room temperature and then analyzed by gas chromatography-mass spectrometry at 0, 1, 7, 15, and 30 days after collection/preparation to look for eventual analyte degradation. Results: Methamphetamine showed no significant changes with respect to the time of collection/ preparation (T0) up to 7 days later (T7), with or without salt mixture addiction. Then a significant degradation occurred in both salted and non salted urine. BE decrease was observed starting from day 1 after sample collection in salted and not salted samples, respectively. Salt addition seemed to reduce at least the initial BE degradation, with a significant difference (p<0.001) at 7 and 15 days of storage. However, the degradation was not more prevented in salted samples at 30 days of storage. A 20% decrease of MOR concentration was observed starting from day 1 after collection/preparation, both in salted and not salted samples with no subsequent decrease. With regard to THCCOOH, a significant decrease was observed starting from 7 days after collection/preparation, with of without adding the salt mixture. However, when comparing salted versus non salted samples at each time point, a statistically significant difference was observed at 7 and 30 days of storage. Conclusion: The results obtained indicate that the degradation of MA, THC-COOH and BE in urine samples kept at room temperature can be slowed by the addition of the salt mixture, whereas it seems to be ineffective in samples containing MOR. This evidence has to be taken into account, in the eventuality of using salted urine to prevent in a certain extent abuse of above-reported drugs of abuse.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results