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The Mount Elgon region of Uganda has coffee farmlands distributed along the slopes of the mountain, in a mosaic of differing crop combinations, and semi-natural vegetation. Thus, there are parcels of varying microclimate that create disparities in occurrence of key insect functional groups. The study quantified the occurrence of Carabidae in 72 coffee farmlands categorized by altitude: low (1400-1499 m.a.s.l), mid (1500-1679 m.a.s.l), and high (1680-2100 m.a.s.l); and farming system: coffee monocrop, coffee+annual crops, coffee+banana, and coffee+banana+shade trees. The results revealed highly significant effects of altitude, farming systems, and the interaction of the two on occurrence of three Carabidae genera (Anisodactylus, Chlaenius, and Harpalus.). The abundance of Harpalus spp. was higher at lower altitudes in coffee monocropped farming systems; Anisodactylus spp. were more abundant at higher altitudes in coffee+annual crop systems; and Chlaenius spp. were highest in the coffee+banana+shade tree system at mid altitudes. The belowground microclimate parameters of soil moisture, pH, EC; and the aboveground diversity of semi-natural vegetation explained some of the differences in occurrence of the different Carabidae genera. This distinctiveness in preference of different genera in the same family hinders collective recommendations but looks to a more pragmatic strategy in nurturing diversity on a holistic scale., (© 2021. Sociedade Entomológica do Brasil.)

To date, the occurrence, fate and toxicity of metal-based NPs in the environment is under investigated. Their unique physicochemical, biological and optical properties, responsible for their advantageous application, make them intrinsically different from their bulk counterpart, raising the issue of their potential toxic specificity or "nanosize effect". The aim of this study was to investigate copper bioaccumulation, subcellular distribution and toxic effect in the marine benthic species Scrobicularia plana exposed to two forms of sediment-associated copper, as nanoparticles (CuO NPs) and as soluble ions (CuCl 2 ). Results showed that the exposure to different copper forms activated specific organism's metal handling strategies. Clams bioaccumulated soluble copper at higher concentrations than those exposed to sediment spiked with CuO NPs. Moreover, CuO NPs exposure elicited a stronger detoxification response mediated by a prompt mobilization of CuO NPs to metal-containing granules as well as a delayed induction of MT-like proteins, which conversely, sequestered soluble copper since the beginning of the exposure at levels significantly different from the control. Eventually, exposure to high concentrations of either copper form led to the same acute toxic effect (100% mortality) but the outcome was delayed in bivalves exposed to CuO NPs suggesting that the mechanisms underlying toxicity were copper form-specific. Indeed, while most of soluble copper was associated to the mitochondrial fraction suggesting an impairment of the ATP synthesis capacity at mitochondrial level, CuO NPs toxicity was most likely caused by the oxidative stress mediated by their bioaccumulation in the enzymatic and mitochondrial metabolically available fractions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The first bioengineered, autologous, dermo-epidermal skin grafts are presently undergoing clinical trials; hence, it is reasonable to envisage the next clinical step at the forefront of plastic and burn surgery, which is the generation of autologous skin grafts that contain vascular plexuses, preformed in vitro. As the importance of the blood, and particularly the lymphatic vascular system, is increasingly recognized, it is attractive to engineer both human blood and lymphatic vessels in one tissue or organ graft. We show here that functional lymphatic capillaries can be generated using three-dimensional hydrogels. Like normal lymphatics, these capillaries branch, form lumen, and take up fluid in vitro and in vivo after transplantation onto immunocompromised rodents. Formation of lymphatic capillaries could be modulated by both lymphangiogenic and anti-lymphangiogenic stimuli, demonstrating the potential usefulness of this system for in vitro testing. Blood and lymphatic endothelial cells never intermixed during vessel development, nor did blood and lymphatic capillaries anastomose under the described circumstances. After transplantation of the engineered grafts, the human lymphatic capillaries anastomosed to the nude rat's lymphatic plexus and supported fluid drainage. Successful preclinical results suggest that these skin grafts could be applied on patients suffering from severe skin defects.

Cleavage factor IA (CF IA), cleavage and polyadenylation factor (CPF), constitute major protein complexes required for pre-mRNA 3' end formation in yeast. The Clp1 protein associates with Pcf11, Rna15 and Rna14 in CF IA but its functional role remained unclear. Clp1 carries an evolutionarily conserved P-loop motif that was previously shown to bind ATP. Interestingly, human and archaean Clp1 homologues, but not the yeast protein, carry 5' RNA kinase activity. We show that depletion of Clp1 in yeast promoted defective 3' end formation and RNA polymerase II termination; however, cells expressing Clp1 with mutant P-loops displayed only minor defects in gene expression. Similarly, purified and reconstituted mutant CF IA factors that interfered with ATP binding complemented CF IA depleted extracts in coupled in vitro transcription/3' end processing reactions. We found that Clp1 was required to assemble recombinant CF IA and that certain P-loop mutants failed to interact with the CF IA subunit Pcf11. In contrast, mutations in Clp1 enhanced binding to the 3' endonuclease Ysh1 that is a component of CPF. Our results support a structural role for the Clp1 P-loop motif. ATP binding by Clp1 likely contributes to CF IA formation and cross-factor interactions during the dynamic process of 3' end formation., (© 2011 Holbein et al.)

1. Increased bronchial smooth muscle contractility with consequent bronchial hyperreactivity are characteristic physiopathological events of asthma. Since magnesium intervenes in calcium transport mechanisms and intracellular phosphorylation reactions, it constitutes an important determinant of the contraction/relaxation state of bronchial smooth muscle. In the present study we investigated the relationship between bronchial reactivity, assessed by methacholine-provocation test, and magnesium concentrations both at extracellular and intracellular levels measured by spectrophotometry. Twenty-two patients with mild-to-moderate asthma and 38 non-asthmatic subjects with allergic rhinitis (24 allergic to Parietaria pollen and 14 allergic to Grass pollen) were recruited to the study. Exclusion criteria included renal failure, hepatic diseases, heart failure and arterial hypertension. 2. The salient finding of our study is that there is a strong positive correlation between bronchial reactivity and the level of intracellular magnesium (r=0.72, P<0.0001), both when the groups are analysed separately or together. Intracellular magnesium concentrations in the group of patients with asthma were significantly lower (1.8+/-0. 01 mmol/l; n=22) when compared with levels in rhinitis subjects allergic to Parietaria (1.9+/-0.01 mmol/l; n=24, P<0.05), and with levels in rhinitis subjects allergic to Grass pollen (2.0+/-0.03 mmol/l; n=14, P<0.05). Serum levels of the ion were similar in all groups. 3. We conclude that the level of intracellular magnesium may be an important determinant of bronchial hyperreactivity, as supported by the significant positive correlation between these two parameters in allergic patients with known bronchial hyperresponsiveness. This finding, in addition to reports of the bronchodilating effects of magnesium administration in patients with asthma, confirms the proposed role of this ion in the pathogenesis and treatment of asthma.

Dentinal Dysplasia Type I is a rare condition affecting the deciduous and permanent dentitions, in which the teeth present with short roots and considerably reduced or obliterated pulp spaces. These features were observed on the radiographs of a patient referred for orthodontic assessment, and an investigation of other members of the family revealed that the father and two siblings were also affected. There were variations in the lengths of the roots in those affected, and thus a subclassification of the dysplasia is proposed.