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2 results on '"Second-generation assay"'

1. Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay.

Author

Hage, Chadi A., Davis, Thomas E., Egan, Lindsey, Parker, Michelle, Fuller, Deanna, LeMonte, Ann M., Durkin, Michelle, Connelly, Patricia, Joseph Wheat, L., Blue-Hnidy, Deborah, and Knox, Kenneth S.

Abstract

Summary: Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis. [Copyright &y& Elsevier]

Published
2007
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2. Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

Author

Michelle Durkin, Michelle Parker, Lindsey Egan, L. Joseph Wheat, Kenneth S. Knox, DeAnna Fuller, Deborah Blue-Hnidy, Ann M. LeMonte, Chadi A. Hage, Patricia Connelly, and Thomas E. Davis

Subjects
Bronchoalveolar lavage, Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, Antigens, Fungal, medicine.medical_treatment, Histoplasma, Enzyme-Linked Immunosorbent Assay, Liver transplantation, Statistics, Nonparametric, Histoplasmosis, Blastomycosis, Second-generation assay, Immunocompromised Host, Antigen, Humans, Medicine, Mycosis, Chi-Square Distribution, Lung Diseases, Fungal, medicine.diagnostic_test, biology, business.industry, Respiratory disease, Middle Aged, medicine.disease, biology.organism_classification, Liver Transplantation, Histoplasma antigen, Immunology, Female, business, Bronchoalveolar Lavage Fluid
Abstract

SummaryAntigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

Published
2007
Full Text
View/download PDF

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