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815 results on '"Seed, M"'

1. Establishing a Fetal Lamb Model for Hypoplastic Left Heart Syndrome—A Feasibility Pilot Study.

Author

Knirsch, W., Haak, M., Jaeggi, E., Seed, M., Hassan, A., Krüger, B., Stoeck, C. T., Schweiger, M., Weisskopf, M., and Chaturvedi, R.

Subjects
HYPOPLASTIC left heart syndrome, FETAL MRI, CARDIAC magnetic resonance imaging, LAMBS, PILOT projects, FEASIBILITY studies, DIFFUSION tensor imaging
Abstract

This article discusses the need for a large fetal animal model of hypoplastic left heart syndrome (HLHS) in order to evaluate and develop medical and surgical management strategies for the condition. The study presents a feasibility pilot study conducted in Switzerland using pregnant ewes to establish this model. The ewes underwent procedures to induce HLHS in their fetuses, and the development of HLHS was confirmed through various imaging techniques. The study found that the model is feasible, although there was a 25% dropout rate due to complications. The researchers believe that this model will provide a valuable research platform for improving the understanding and treatment of HLHS. [Extracted from the article]

Published
2024
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5. MRI characterization of hemodynamic patterns of human fetuses with cyanotic congenital heart disease

Author

Sun, L., primary, van Amerom, J. F. P., additional, Marini, D., additional, Portnoy, S., additional, Lee, F.‐T., additional, Saini, B. S., additional, Lim, J. M., additional, Aguet, J., additional, Jaeggi, E., additional, Kingdom, J. C., additional, Macgowan, C. K., additional, Miller, S. P., additional, Huang, G., additional, and Seed, M., additional

Published
2021
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11. Communicating Risk Reductions

Author

McMurray, J., Galton, D. J., Seed, M., Edwards, Adrian, Elwyn, Glyn, Stott, Nigel, Steel, Nicholas, and Skolbekken, John-Arne

Published
1999

13. New Frontiers in Compound-Specific δ2H Analysis

Author

Lengger, S., primary, Kelly, S., additional, Taylor, K.W.R., additional, Weber, Y., additional, Kopf, S., additional, Berstan, R., additional, Seed, M., additional, Bull, I., additional, Meyser, J., additional, Leavitt, W., additional, Blewett, J., additional, Abrahim, A., additional, Cannavan, A., additional, Pearson, A., additional, and Pancost, R., additional

Published
2021
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25. OC01.05: Feasibility of the short‐term physiologic support of the late‐gestation miniature pig fetus using umbilical cannulation, a pumpless extreacorporeal membrane oxygenator and fluid incubation

Author

Taniguchi, L.K., primary, Haller, C., additional, Chaturvedi, R., additional, Mroczek, D., additional, Crawford, L., additional, Foreman, C., additional, Belik, J., additional, McVey, M., additional, Floh, A., additional, Macgowan, C., additional, Estrada, M., additional, Cho, K., additional, Aujla, T., additional, Ren, J., additional, Stirrat, E., additional, Oatley, H., additional, Seed, M., additional, and Morrison, J., additional

Published
2019
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28. A study of prostacyclin production by the pregnant myometrium

Author

Seed, M. P.

Subjects
615.1, Pharmacology & pharmacy & pharmaceutical chemistry
Abstract

The rat and human pregnant myometrium and human placenta, when chopped and incubated in Tris-buffered saline (25%, 50% & 50% w/v respectively) released materials with anti-aggregatory activity. The myometrial activities were stable at pH 12.0, and unstable at pH 4.0. Optimum conditions for the release of the anti-aggregatory activities were 15, 30 and 15 minutes incubation respectively at 20°C at pH 8.0. The release of anti-aggregatory activity by human myometrium was stimulated by arachidonic acid and phospholipase A2 (PLA2) and inhibited by indomethacin. Human myometrial incubate did not possess adenosine- diphosphatase activity. This anti-aggregatory activity released by pregnant rat and human myometria was attributed to prostacyclin (PGI2). Human placental anti-aggregatory activity was unstable at both pH 12.0 and pH 4.0, was not inhibited by administration of indomethacin and had the ability to degrade adenosine diphosphate. PGI2 synthesis by human pregnant myometrium was low up to week 37 of pregnancy and then increased progressively to a maximum at week 40 (term). Two patients who did not progress to labour by week 42 had a low capacity to release PGI2. Incubation of human myometrial tissue with oxytocin and ergometrine did not alter PGI2 synthesis. PGI2 inhibited the spontaneous contractility of human pregnant myometrium bathed in Krebs' solution in 3/4 samples and reduced basal tone in 2/4. Studies on pregnant rat myometrial PGI2 synthesis showed that removal of the deciduum by scraping with a microscope slide resulted in greatly increased synthesis of PGI2 compared to teasing. Chopping increased the rate of synthesis of the scraped samples, and maximal synthesis of the teased samples. Pre-incubation of scraped myometrial tissue with uterine stimulant drugs (oxytocin, angiotensin & 5-hydroxytryptamine [5-HT]) stimulated PGI2 synthesis in a dose related fashion. Methysergide, whilst reducing synthesis, inhibited the stimulant effect of 5-HT. The uterine relaxant salbutamol inhibited synthesis by day 21 but not day 19 pregnant myometerium, and was antagonised by propranolol. The adenylate cyclase stimulant, forskolin, reduced PGI2 synthesis. Rat uterine homogenate, when incubated in HEPES buffer (pH 8.0; + Triton X-100) released H-oleic acid from I'-oleoyl, 2'-[3H]-oleoyl- phosphatidylcholine (H-dPC). Two methods were assessed for the separation of released oleic acid from the 3H-dPC. Thin layer chromatography was found to be the method of choice. The release of 3H-oleic acid by late pregnant rat uterine homogenate acid was Ca++ dependent, protein dependent, had a pH optimum at pH 8.0 and was inhibited by mepacrine and tetracaine. This activity was attributed to PLA2. Perfusion of the uterine vascular bed with Krebs' solution to remove blood elements increased activity as measured by this method.

Published
1983

33. Fetal XCMR: a numerical phantom for fetal cardiovascular magnetic resonance imaging

Author

Roy, C.W., Marini, D., Segars, W.P., Seed, M., and Macgowan, C.K.

Subjects
embryonic structures, cardiovascular system, cardiovascular diseases, Anatomic Landmarks, Computer Simulation, Female, Fetal Heart/diagnostic imaging, Humans, Magnetic Resonance Imaging/instrumentation, Models, Cardiovascular, Numerical Analysis, Computer-Assisted, Phantoms, Imaging, Predictive Value of Tests, Pregnancy, Prenatal Diagnosis/instrumentation, Reproducibility of Results, Fetal cardiovascular magnetic resonance imaging, Golden angle radial, Motion correction, Numerical simulation, Physiological motion, Post-processing
Abstract

Validating new techniques for fetal cardiovascular magnetic resonance (CMR) is challenging due to random fetal movement that precludes repeat measurements. Consequently, fetal CMR development has been largely performed using physical phantoms or postnatal volunteers. In this work, we present an open-source simulation designed to aid in the development and validation of new approaches for fetal CMR. Our approach, fetal extended Cardiac-Torso cardiovascular magnetic resonance imaging (Fetal XCMR), builds on established methods for simulating CMR acquisitions but is tailored toward the dynamic physiology of the fetal heart and body. We present comparisons between the Fetal XCMR phantom and data acquired in utero, resulting in image quality, anatomy, tissue signals and contrast. Existing extended Cardiac-Torso models are modified to create maternal and fetal anatomy, combined according to simulated motion, mapped to CMR contrast, and converted to CMR data. To provide a comparison between the proposed simulation and experimental fetal CMR images acquired in utero, images from a typical scan of a pregnant woman are included and simulated acquisitions were generated using matching CMR parameters, motion and noise levels. Three reconstruction (static, real-time, and CINE), and two motion estimation methods (translational motion, fetal heart rate) from data acquired in transverse, sagittal, coronal, and short-axis planes of the fetal heart were performed to compare to in utero acquisitions and demonstrate feasibility of the proposed simulation framework. Overall, CMR contrast, morphologies, and relative proportions of the maternal and fetal anatomy are well represented by the Fetal XCMR images when comparing the simulation to static images acquired in utero. Additionally, visualization of maternal respiratory and fetal cardiac motion is comparable between Fetal XCMR and in utero real-time images. Finally, high quality CINE image reconstructions provide excellent delineation of fetal cardiac anatomy and temporal dynamics for both data types. The fetal CMR phantom provides a new method for evaluating fetal CMR acquisition and reconstruction methods by simulating the underlying anatomy and physiology. As the field of fetal CMR continues to grow, new methods will become available and require careful validation. The fetal CMR phantom is therefore a powerful and convenient tool in the continued development of fetal cardiac imaging.

Published
2019

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