Search

A theoretical formulation utilizing incremental inductance method has been developed to calculate the coupling coefficient, London penetration depth, and surface resistance of a coplanar waveguide resonator fabricated from films of superconducting YBCO material. Experimental data of the reflection coefficient as a function of temperature and frequency agree reasonably well with calculations.

We investigated risk factors for severe acute lower respiratory infections (ALRI) among hospitalised children <2 years, with a focus on the interactions between virus and age. Statistical interactions between age and respiratory syncytial virus (RSV), influenza, adenovirus (ADV) and rhinovirus on the risk of ALRI outcomes were investigated. Of 1780 hospitalisations, 228 (12.8%) were admitted to the intensive care unit (ICU). The median (range) length of stay (LOS) in hospital was 3 (1–27) days. An increase of 1 month of age was associated with a decreased risk of ICU admission (rate ratio (RR) 0.94; 95% confidence intervals (CI) 0.91–0.98) and with a decrease in LOS (RR 0.96; 95% CI 0.95–0.97). Associations between RSV, influenza, ADV positivity and ICU admission and LOS were significantly modified by age. Children <5 months old were at the highest risk from RSV-associated severe outcomes, while children >8 months were at greater risk from influenza-associated ICU admissions and long hospital stay. Children with ADV had increased LOS across all ages. In the first 2 years of life, the effects of different viruses on ALRI severity varies with age. Our findings help to identify specific ages that would most benefit from virus-specific interventions such as vaccines and antivirals. [ABSTRACT FROM AUTHOR]

Human respiratory syncytial virus (RSV) is a major cause of acute respiratory infection. In 2020, RSV was effectively eliminated from the community in New Zealand due to non-pharmaceutical interventions (NPI) used to control the spread of COVID-19. However, in April 2021, following a brief quarantine-free travel agreement with Australia, there was a large-scale nationwide outbreak of RSV that led to reported cases more than five times higher, and hospitalisations more than three times higher, than the typical seasonal pattern. In this study, we generated 1,471 viral genomes of both RSV-A and RSV-B sampled between 2015 and 2022 from across New Zealand. Using a phylodynamics approach, we used these data to better understand RSV transmission patterns in New Zealand prior to 2020, and how RSV became re-established in the community following the relaxation of COVID-19 restrictions. We found that in 2021, there was a large epidemic of RSV in New Zealand that affected a broader age group range compared to the usual pattern of RSV infections. This epidemic was due to an increase in RSV importations, leading to several large genomic clusters of both RSV-A ON1 and RSV-B BA9 genotypes in New Zealand. However, while a number of importations were detected, there was also a major reduction in RSV genetic diversity compared to pre-pandemic seasonal outbreaks. These genomic clusters were temporally associated with the increase of migration in 2021 due to quarantine-free travel from Australia at the time. The closest genetic relatives to the New Zealand RSV genomes, when sampled, were viral genomes sampled in Australia during a large, off-season summer outbreak several months prior, rather than cryptic lineages that were sustained but not detected in New Zealand. These data reveal the impact of NPI used during the COVID-19 pandemic on other respiratory infections and highlight the important insights that can be gained from viral genomes.

Background: New Zealand's (NZ) complete absence of community transmission of influenza and respiratory syncytial virus (RSV) after May 2020, likely due to COVID-19 elimination measures, provided a rare opportunity to assess the impact of border restrictions on common respiratory viral infections over the ensuing 2 years., Methods: We collected the data from multiple surveillance systems, including hospital-based severe acute respiratory infection surveillance, SHIVERS-II, -III and -IV community cohorts for acute respiratory infection (ARI) surveillance, HealthStat sentinel general practice (GP) based influenza-like illness surveillance and SHIVERS-V sentinel GP-based ARI surveillance, SHIVERS-V traveller ARI surveillance and laboratory-based surveillance. We described the data on influenza, RSV and other respiratory viral infections in NZ before, during and after various stages of the COVID related border restrictions., Results: We observed that border closure to most people, and mandatory government-managed isolation and quarantine on arrival for those allowed to enter, appeared to be effective in keeping influenza and RSV infections out of the NZ community. Border restrictions did not affect community transmission of other respiratory viruses such as rhinovirus and parainfluenza virus type-1. Partial border relaxations through quarantine-free travel with Australia and other countries were quickly followed by importation of RSV in 2021 and influenza in 2022., Conclusion: Our findings inform future pandemic preparedness and strategies to model and manage the impact of influenza and other respiratory viral threats., (© 2024 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.)

The failure to mount an antibody response following viral infection or seroconversion failure is a largely underappreciated and poorly understood phenomenon. Here, we identified immunologic markers associated with robust antibody responses after influenza virus infection in two independent human cohorts, SHIVERS and FLU09, based in Auckland, New Zealand and Memphis, Tennessee, USA, respectively. In the SHIVERS cohort, seroconversion significantly associates with (1) hospitalization, (2) greater numbers of proliferating, activated CD4 + T cells, but not CD8 + T cells, in the periphery during the acute phase of illness, and (3) fewer inflammatory monocytes (CD14 hi CD16 + ) by convalescence. In the FLU09 cohort, fewer CD14 hi CD16 + monocytes during early illness in the nasal mucosa were also associated with the generation of influenza-specific mucosal immunoglobulin A (IgA) and IgG antibodies. Our study demonstrates that seroconversion failure after infection is a definable immunological phenomenon, associated with quantifiable cellular markers that can be used to improve diagnostics, vaccine efficacy, and epidemiologic efforts., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)

Despite evidence that antibodies targeting the influenza virus neuraminidase (NA) protein can be protective and are broadly cross-reactive, the immune response to NA during infection is poorly understood compared to the response to hemagglutinin (HA) protein. As such, we compared the antibody profile to HA and NA in two naturally infected human cohorts in Auckland, New Zealand: (i) a serosurvey cohort, consisting of pre- and post-influenza season sera from PCR-confirmed influenza cases ( n  = 50), and (ii) an immunology cohort, consisting of paired sera collected after PCR-confirmation of infection ( n  = 94). The induction of both HA and NA antibodies in these cohorts was influenced by age and subtype. Seroconversion to HA was more frequent in those <20 years old (yo) for influenza A (serosurvey, P  = 0.01; immunology, P  = 0.02) but not influenza B virus infection. Seroconversion to NA was not influenced by age or virus type. Adults ≥20 yo infected with influenza A viruses were more likely to show NA-only seroconversion compared to children (56% versus 14% [5 to 19 yo] and 0% [0 to 4 yo], respectively). Conversely, children infected with influenza B viruses were more likely than adults to show NA-only seroconversion (88% [0 to 4 yo] and 75% [5 to 19 yo] versus 40% [≥20 yo]). These data indicate a potential role for immunological memory in the dynamics of HA and NA antibody responses. A better mechanistic understanding of this phenomenon will be critical for any future vaccines aimed at eliciting NA immunity. IMPORTANCE Data on the immunologic responses to neuraminidase (NA) is lacking compared to what is available on hemagglutinin (HA) responses, despite growing evidence that NA immunity can be protective and broadly cross-reactive. Understanding these NA responses during natural infection is key to exploiting these properties for improving influenza vaccines. Using two community-acquired influenza cohorts, we showed that the induction of both HA and NA antibodies after infection is influenced by age and subtypes. Such response dynamics suggest the influence of immunological memory, and understanding how this process is regulated will be critical to any vaccine effort targeting NA immunity., (Copyright © 2020 Wong et al.)

Cytokines and chemokines are key signaling molecules of the immune system. Recent technological advances enable measurement of multiplexed cytokine profiles in biological samples. These profiles can then be used to identify potential biomarkers of a variety of clinical phenotypes. However, testing for such associations for each cytokine separately ignores the highly context-dependent covariation in cytokine secretion and decreases statistical power to detect associations due to multiple hypothesis testing. Here we present CytoMod-a novel data-driven approach for analysis of cytokine profiles that uses unsupervised clustering and regression to identify putative functional modules of co-signaling cytokines. Each module represents a biosignature of co-signaling cytokines. We applied this approach to three independent clinical cohorts of subjects naturally infected with influenza in which cytokine profiles and clinical phenotypes were collected. We found that in two out of three cohorts, cytokine modules were significantly associated with clinical phenotypes, and in many cases these associations were stronger than the associations of the individual cytokines within them. By comparing cytokine modules across datasets, we identified cytokine "cores"-specific subsets of co-expressed cytokines that clustered together across the three cohorts. Cytokine cores were also associated with clinical phenotypes. Interestingly, most of these cores were also co-expressed in a cohort of healthy controls, suggesting that in part, patterns of cytokine co-signaling may be generalizable. CytoMod can be readily applied to any cytokine profile dataset regardless of measurement technology, increases the statistical power to detect associations with clinical phenotypes and may help shed light on the complex co-signaling networks of cytokines in both health and infection.

Background: Pregnant women are prioritized for seasonal influenza vaccination, but the evidence on the risk of influenza during pregnancy that is used to inform these policies is limited., Methods: Individual-level administrative data sets and active surveillance data were joined to estimate influenza-associated hospitalization and outpatient visit rates by pregnancy, postpartum, and trimester status., Results: During 2012-2015, 46 of 260 (17.7%) influenza-confirmed hospitalizations for acute respiratory infection and 13 of 294 (4.4%) influenza-confirmed outpatient visits were among pregnant and postpartum women. Pregnant and postpartum women experienced higher rates of influenza-associated hospitalization than nonpregnant women overall (rate ratio [RR], 3.4; 95% confidence interval [CI], 2.5-4.7) and by trimester (first, 2.5 [95% CI, 1.2-5.4]; second, 3.9 [95% CI, 2.4-6.3]; and third, 4.8 [95% CI, 3.0-7.7]); the RR for the postpartum period was 0.7 (95% CI, 3.0-7.7). Influenza A viruses were associated with an increased risk (RR for 2009 pandemic influenza A[H1N1] virus, 5.3 [95% CI, 3.2-8.7]; RR for influenza A(H3N2) virus, 3.0 [95% CI, 1.8-5.0]), but influenza B virus was not (RR, 1.8; 95% CI, .7-4.6). Influenza-associated hospitalization rates in pregnancy were significantly higher for Māori women (RR, 3.2; 95% CI, 1.3-8.4), compared with women of European or other ethnicity. Similar risks for influenza-confirmed outpatient visits were not observed., Conclusion: Seasonal influenza poses higher risks of hospitalization among pregnant women in all trimesters, compared with nonpregnant women. Hospitalization rates vary by influenza virus type and ethnicity among pregnant women., (© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)

Background: Understanding the attack rate of influenza infection and the proportion who become ill by risk group is key to implementing prevention measures. While population-based studies of antihemagglutinin antibody responses have been described previously, studies examining both antihemagglutinin and antineuraminidase antibodies are lacking., Methods: In 2015, we conducted a seroepidemiologic cohort study of individuals randomly selected from a population in New Zealand. We tested paired sera for hemagglutination inhibition (HAI) or neuraminidase inhibition (NAI) titers for seroconversion. We followed participants weekly and performed influenza polymerase chain reaction (PCR) for those reporting influenza-like illness (ILI)., Results: Influenza infection (either HAI or NAI seroconversion) was found in 321 (35% [95% confidence interval, 32%-38%]) of 911 unvaccinated participants, of whom 100 (31%) seroconverted to NAI alone. Young children and Pacific peoples experienced the highest influenza infection attack rates, but overall only a quarter of all infected reported influenza PCR-confirmed ILI, and one-quarter of these sought medical attention. Seroconversion to NAI alone was higher among children aged <5 years vs those aged ≥5 years (14% vs 4%; P < .001) and among those with influenza B vs A(H3N2) virus infections (7% vs 0.3%; P < .001)., Conclusions: Measurement of antineuraminidase antibodies in addition to antihemagglutinin antibodies may be important in capturing the true influenza infection rates.

Background: The immunologic factors underlying severe influenza are poorly understood. To address this, we compared the immune responses of influenza-confirmed hospitalized individuals with severe acute respiratory illness (SARI) to those of nonhospitalized individuals with influenza-like illness (ILI)., Methods: Peripheral blood lymphocytes were collected from 27 patients with ILI and 27 with SARI, at time of enrollment and then 2 weeks later. Innate and adaptive cellular immune responses were assessed by flow cytometry, and serum cytokine levels were assessed by a bead-based assay., Results: During the acute phase, SARI was associated with significantly reduced numbers of circulating myeloid dendritic cells, CD192+ monocytes, and influenza virus-specific CD8+ and CD4+ T cells as compared to ILI. By the convalescent phase, however, most SARI cases displayed continued immune activation characterized by increased numbers of CD16+ monocytes and proliferating, and influenza virus-specific, CD8+ T cells as compared to ILI cases. SARI was also associated with reduced amounts of cytokines that regulate T-cell responses (ie, interleukin 4, interleukin 13, interleukin 12, interleukin 10, and tumor necrosis factor β) and hematopoiesis (interleukin 3 and granulocyte-macrophage colony-stimulating factor) but increased amounts of a proinflammatory cytokine (tumor necrosis factor α), chemotactic cytokines (MDC, MCP-1, GRO, and fractalkine), and growth-promoting cytokines (PDGFBB/AA, VEGF, and EGF) as compared to ILI., Conclusions: Severe influenza cases showed a delay in the peripheral immune activation that likely led prolonged inflammation, compared with mild influenza cases., (© The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)

Key Message: Using a combination of phenotypic screening and molecular, statistical, and linkage analyses, we have mapped a dominant soybean rust resistance gene in soybean PI 567104B. Asian soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi Syd. and P. Syd., is one of the most economically important diseases that affect soybean production worldwide. A long-term strategy for minimizing the effects of SBR is the development of genetically resistant cultivars. The objectives of the study were to identify the location of a rust-resistance (Rpp) gene(s) in plant introduction (PI) 567104B, and to determine if the gene(s) in PI 567104B was different from previously mapped Rpp loci. The progeny of the cross of 'IAR 2001 BSR' × PI 567104B was phenotyped from field assays of the F 2:3 and F 4:5 generations and from a growth chamber assay of 253 F 5:6 recombinant inbred lines (RILs). For the growth chamber, the phenotyping was conducted by inoculation with a purified 2006 fungal isolate from Mississippi. A resistance gene locus on PI 567104B was mapped to a region containing the Rpp6 locus on chromosome 18. The high level of resistance of F 1 plants from two other crosses with PI 567104B as one of the parents indicated that the gene from PI 567104B was dominant. The interval containing the gene is flanked by the simple sequence repeat (SSR) markers Satt131 and Satt394, and includes the SSR markers BARCSOYSSR&#95;18&#95;0331 and BARCSOYSSR&#95;18&#95;0380. The results also indicated that the resistance gene from PI 567104B is different from the Rpp1 to the Rpp4 genes previously identified. To determine if the gene from PI 567104B is different from the Rpp6 gene from PI 567102B, additional research will be required.

Background: Recent experience with pandemic influenza A(H1N1)pdm09 highlighted the importance of global surveillance for severe respiratory disease to support pandemic preparedness and seasonal influenza control. Improved surveillance in the southern hemisphere is needed to provide critical data on influenza epidemiology, disease burden, circulating strains and effectiveness of influenza prevention and control measures. Hospital-based surveillance for severe acute respiratory infection (SARI) cases was established in New Zealand on 30 April 2012. The aims were to measure incidence, prevalence, risk factors, clinical spectrum and outcomes for SARI and associated influenza and other respiratory pathogen cases as well as to understand influenza contribution to patients not meeting SARI case definition., Methods/design: All inpatients with suspected respiratory infections who were admitted overnight to the study hospitals were screened daily. If a patient met the World Health Organization's SARI case definition, a respiratory specimen was tested for influenza and other respiratory pathogens. A case report form captured demographics, history of presenting illness, co-morbidities, disease course and outcome and risk factors. These data were supplemented from electronic clinical records and other linked data sources., Discussion: Hospital-based SARI surveillance has been implemented and is fully functioning in New Zealand. Active, prospective, continuous, hospital-based SARI surveillance is useful in supporting pandemic preparedness for emerging influenza A(H7N9) virus infections and seasonal influenza prevention and control.

Fusarium head blight of barley (Hordeum vulgare) is a devastating disease in many countries. We undertook a study to identify barley cultivars, if any, that are resistant to Fusarium head blight and deoxynivalenol (DON) accumulation and to determine if DON concentration is correlated with other plant traits in Eastern Canada and China. Barley cultivars were grown in the field under artificial inoculation conditions at two locations (Charlottetown and Ottawa) in Canada during two summers and at Hangzhou in China during two winters. Seed samples were collected for DON analysis from the barley performance trial at five locations in Ontario. None of the 64 barley cultivars were immune to Fusarium head blight infection. Two-row cultivars, however, were significantly more resistant to Fusarium head blight infection and DON accumulation than six-row cultivars. Three cultivars (Island, AC Alberte, and Chevron) were found to be most resistant, as they were consistently low in Fusarium head blight incidence and DON concentration in both Eastern Canada and China. In six-row barley, DON concentration was correlated positively with Fusarium head blight incidence at both Charlottetown and Ottawa, and it was negatively correlated with plant height at Ottawa. DON concentration and heading date were not consistently correlated. Barley yellow dwarf and powdery mildew appeared to have very little effect on Fusarium head blight infection. Susceptibility to DON accumulation did not result in low yield under natural infection conditions in Ontario. Cultivar × location interactions for DON concentration, Fusarium head blight incidence, and heading date were significant.

The purpose of this study was to compare the preferred low-back motion of normal subjects and low-back-pain patients. Each subject performed a maximum isometric flexion trial followed by repeated flexion and extension against a relative resistance set at 50% of the recorded maximum flexion isometric torque. The subjects were instructed to move at their own pace through their preferred movement range until either exhaustion or for 120s. The results showed that the groups differed significantly in their preferred motion characteristics, although the performances were equally consistent.

This study was conducted to assess the reliability of torque measurements across different trials and different test sessions. Each trial consisted of three maximal, reciprocal contractions of the knee extensors and flexors at test velocities of 60 and 180 degrees /sec. Three trials were conducted on each of three test sessions, with each session separated by 48 hours. Results indicate that reliability of reciprocal isokinetic testing of the knee can be improved by 1) testing in more than one occasion, 2) testing more than three trials during a single session if testing during more than one session is impossible, and 3) allowing the subject to become familiar with the isokinetic test procedure and to warm up adequately. Data obtained in this study indicated performance variability was predominantly associated with between subject differences and secondarily related to within subject changes over trials and days. Dynamometer calibration remained stable across all test sessions, indicating that the method was reliable for recording torque output during all testing procedures. J Orthop Sports Phys Ther 1991;14(3):121-127.

The purposes of this study were to identify the loading effects on preferred low-back motion and to determine the consistency of preferred low-back motion. Fifteen male and female volunteers served as subjects. Each subject performed four trials of repeated flexion and extension until either the subject became exhausted or for 120s. Each trial corresponded to a relative load of 0, 25, 50, and 75% of the subject's prerecorded maximum torque in isometric flexion. The subjects were instructed to perform the movements at their own pace and through their preferred movement range. The results showed that above the 25% resistance level, the increased loads were accompanied by decreased velocities, with the changes due to the cycle temporal characteristics. The increased loads also resulted in decreased cycle excursions, with these changes due to reductions in the extension endpoints. With increased resistances the descriptive parameters became more variable, with a trend of increased variability toward the latter portion of a trial.

The purpose of this study was the gathering of normative data on the low back for normal males and females utilizing the lsostation BlOO (Isotechnologies, Hillsborough, NC). Included in the study were 110 males and 50 females who were evaluated in a series of four tests. Data were gathered three-dimensionally for the following: a) range of motion with light resistance in flexion/extension, rotation to right and left, and lateral flexion to right and left; b) repetitive flexion/extension for a 20-second time period at a light resistance; c) repetitive flexion/extension for 20 seconds with a heavy resistance; and d) a modified maximum lift. In all tests, males had higher values than females; however, females had greater standard deviations than males in all tests. Thus, there were some females who had higher values than some males. There were weak correlations between peak torque output in the flexion/extension axes in relation to height and body weight in tests a and b where light resistance was used. For tests c and d the correlation improved. Also of interest, consistent activity was noted on the torque and range of motion graphs of rotation and lateral flexion during repetitive flexion/extension tests. These patterned torques and ranges of motion noted in rotation and lateral flexion coincide with the graph patterns of flexion/extension. This accessory motion may be indicative of effort or pathology. Crossover activity needs to be researched further as a possible determinant of effort levels and three-dimensional reproducibility. J Orthop Sports Phys Ther 1987;9(4):141-155.

The Reference Department of The Milton S. Hershey Medical Center Library has offered SDI service to the faculty for over three and one-half years. This study traces the development of SDI services from a manual Current Awareness Service (CAS), with the incorporation of Index Medicus photocopies, to the National Library of Medicine's SDILINE (Selective Dissemination of Information On-LINE). Over 24% of the Medical Center's faculty currently receive SDILINE service. Through questionnaires, the two systems are evaluated and compared. There was 100% return on both questionnaires. Both the manual CAS and SDILINE were favorably received by the faculty, but SDILINE was chosen unanimously as the preferred service. A subscription fee for SDILINE did not significantly affect the system's use. Conclusions reached through the questionnaires indicate that constant evaluation of subject profiles and interaction between the librarian and the user are necessary for optimum use of any SDI service.

The purpose of this study was to document the torque and range of motion parameters generated on the lsostation B100 by subacute low back pain patients 2-7 days postinjury. Abnormal values found in this study were compared to normal values found in a previous study. One hundred and forty-three males and 29 females were evaluated in a predetermined combination of three tests. These included: a) range of motion in each plane, b) repetitive flexion/extension, and c) a modified maximum lift. The results of the investigation indicate significant differences (p = 0.01) in parameters generated by the lsostation B100 between normals and abnormals in each test. Discrepancies between normals and abnormals were greater for females than males. Torque output was most significantly affected (p = 0.01) in the axis of flexion/extension for both males and females. Range of motion was most significantly affected (p = 0.01) in extension for both males and females. In test b (the flexion/extension repetitive test), the most significant (p = 0.01) percent reduction in torque and range of motion values occurred in the secondary axis of rotation and lateral flexion rather than flexion/extension. This reduced crossover activity, crosstalk, may be an indicator of guarded effort, poor effort or both. The lsostation B100 is a valuable tool as it provides objective information on torque output and range of motion for the low back.J Orthop Sports Phys Ther 1988;10(4):121-133.

A total of 200 men and 100 women with no prior history of low back pain were evaluated by a series of 6 different tests on the Isostation B200 (Isotechnologies, Hillsborough, NC, U.S.A.) to obtain normative data on the functional performance of the low back. The data collected in this descriptive study were analyzed for significant patterns and differences between an asymptomatic male and female population. In the range of motion test, male subjects demonstrated greater range of motion in all planes; however, values for women showed greater standard deviations. In the maximum isometric test, male torque values exceeded female values, but male values showed greater standard deviations. A rank order of parameter values was made from values recorded during repetitive tests for both men and women. Coupling patterns observed during single-plane repetitive tests allowed secondary and tertiary axes to be identified. Torque values generated over the set resistance were calculated for the repetitive tests conducted in three different planes. A positive curvilinear, concave downward relationship was observed when these values were plotted against the predetermined resistance settings. Future research will study the effect resistance settings on maximum torque output and the role of velocity as a key discriminator between normal and abnormal populations.