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This study explored the roles of activity versus state direction in how Indonesians adhered to COVID-19 preventive behaviour using the theory of planned behaviour (TPB). A total of 615 participants were gathered through a web questionnaire containing 68 questions. Structural equation modelling (SEM) was used to determine the causal relationships of the latent variable construct. The results revealed that information sources positively influence both attitudes and subjective norms toward preventive behaviour. The results also support self-regulation as a moderator of the main intent-behaviour relationship. Moreover, state-oriented people cannot self-regulate their behaviour to comply with COVID-19 protocols. This investigation provides productive experiences for strategists and managers to improve COVID-19 compliance. This paper's analysis also contributes to literature by revealing the intention to comply with COVID-19 protocols by emphasizing the role of self-regulation. The findings are relevant to practitioners and policymakers in the field of public health and managing of human behavior, particularly to improve of compliance with COVID-19 prevention. Therefore, governments or public health organizations can be utilized to improve the number of individuals who practice COVID-19 preventative behaviours now and in the future, but various factors must be addressed. Future directions and recommendations for improving TPB are suggested., Competing Interests: The authors declare no conflict of interest., (© 2022 The Author(s).)

The green microalga Botryococcus braunii produces hydrocarbon oils at 25-75% of its dry weight and is a promising source of biofuel feedstock. Few studies have examined this species' ecology in natural habitats, and few wild genetic resources have been collected due to difficulties caused by its low abundance in nature. This study aimed to develop a real-time PCR assay for specific detection and quantification of this alga in natural environments and to quantify spatiotemporal variations of wild B. braunii populations in a tropical pond. We designed PCR primers toward the hydrocarbon biosynthesis gene SSL-3 and examined amplification specificity and PCR efficiency with 70 wild strains newly isolated from various environments. The results demonstrated that this PCR assay specifically amplified B. braunii DNA, especially that of B-race strains, and can be widely used to detect wild B. braunii strains in temperate and tropical habitats. Field-testing in a tropical pond suggested a diurnal change in the abundance of B. braunii in surface water and found B. braunii not only in surface water, but also at 1-1.5 m deep and in bottom sediments. This method can contribute to efficient genetic resource exploitations and may also help elucidate the unknown ecology of B. braunii.