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To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.

An application build describes compiling and linking the source code of a developed application to libraries and executables. A Continuous Integration (CI) build executes such a build after the source code has been changed and tries to integrate the changes into the existing application. Such CI builds are executed automatically and include automated software tests, which give the developer the assurance that the changes are technically correct. When the time between the discovery of a test failure and the notification to the developer about it is too long, the development process will be impacted negatively and the beneficial effects of CI decrease. Even though several companies already have CI systems that display all events of a single CI build on a terminal during runtime, bigger applications often involve several CI builds in a single CI pipeline to integrate code changes. Observing the events of these CI builds during runtime might require concurrent monitoring of several different terminals. This thesis overcomes this issue by developing a Proof of Concept (PoC) which streams the test failures of a whole CI pipeline in real-time to the developer. To show the feasibility of real-time failure event streaming of CI builds, the PoC is implemented within Spotify’s CI for clientfacing applications. The issues highlighted by this initial PoC will help to refine the whole CI practice. Furthermore, the faster feedback cycles realised by this PoC will lead to a productivity, efficiency and happiness increase for the involved developers and, eventually, higher quality of the developed software., Ett applikationsbygge beskriver kompilering och länkning av källkod för en utvecklad applikation till bibliotek och körbara filer. Ett Kontinuerlig Integrerings (CI)-bygge kör en sådan bygge efter att källkoden har ändrats och försöker integrera ändringarna i den befintliga applikationen. Sådana CIbyggen exekveras automatiskt och inkluderar automatiserade mjukvarutester, som ger utvecklaren en försäkran om att ändringarna är tekniskt korrekta. När tiden mellan upptäckten av ett testfel och meddelandet till utvecklaren om det är för lång kommer utvecklingsprocessen att påverkas negativt och de fördelaktiga effekterna av CI minskar. Även om flera företag redan har CIsystem som visar alla händelser av ett enskilt CI-bygge i en terminal under körning, involverar större applikationer ofta flera CI-byggen i en och samma CI-pipeline för att integrera kodändringar. Att observera händelserna i dessa CI-byggen under körning kan kräva jämlöpande övervakning av flera olika terminaler. Den här avhandlingen övervinner detta problem genom att utveckla en PoC som strömmar testfelen för en hel CI-pipeline i realtid till utvecklaren. För att visa genomförbarheten av strömning av felhändelser i realtid av CIbyggnader implementeras PoC i Spotifys CI för klientvända applikationer. De problem som lyfts fram av denna första PoC kommer att bidra till att förfina hela CI-praxisen. Dessutom kommer de snabbare återkopplingscyklerna som realiseras av denna PoCatt leda till ökad produktivitet, effektivitet och glädje för de inblandade utvecklarna och, så småningom, högre kvalitet på den utvecklade mjukvaran.

application/pdf, To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.

To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.