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138 results on '"Seita, Yasunari"'

10. Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease

Author

Tsukiyama, Tomoyuki, Kobayashi, Kenichi, Nakaya, Masataka, Iwatani, Chizuru, Seita, Yasunari, Tsuchiya, Hideaki, Matsushita, Jun, Kitajima, Kahoru, Kawamoto, Ikuo, Nakagawa, Takahiro, Fukuda, Koji, Iwakiri, Teppei, Izumi, Hiroyuki, Itagaki, Iori, Kume, Shinji, Maegawa, Hiroshi, Nishinakamura, Ryuichi, Nishio, Saori, Nakamura, Shinichiro, Kawauchi, Akihiro, and Ema, Masatsugu

Published
2019
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12. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells

Author

Seita, Yasunari, primary, Cheng, Keren, additional, McCarrey, John R, additional, Yadu, Nomesh, additional, Cheeseman, Ian H, additional, Bagwell, Alec, additional, Ross, Corinna N, additional, Santana Toro, Isamar, additional, Yen, Li-hua, additional, Vargas, Sean, additional, Navara, Christopher S, additional, Hermann, Brian P, additional, and Sasaki, Kotaro, additional

Published
2023
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13. Author response: Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells

Author

Seita, Yasunari, primary, Cheng, Keren, primary, McCarrey, John R, additional, Yadu, Nomesh, additional, Cheeseman, Ian H, additional, Bagwell, Alec, additional, Ross, Corinna N, additional, Santana Toro, Isamar, additional, Yen, Li-hua, additional, Vargas, Sean, additional, Navara, Christopher S, additional, Hermann, Brian P, additional, and Sasaki, Kotaro, additional

Published
2022
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15. Reconstitution of human adrenocortical specification and steroidogenesis using induced pluripotent stem cells

Author

Sakata, Yuka, primary, Cheng, Keren, additional, Mayama, Michinori, additional, Seita, Yasunari, additional, Detlefsen, Andrea J., additional, Mesaros, Clementina A., additional, Penning, Trevor M., additional, Shishikura, Kyosuke, additional, Yang, Wenli, additional, Auchus, Richard J., additional, Strauss, Jerome F., additional, and Sasaki, Kotaro, additional

Published
2022
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16. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells

Author

Seita, Yasunari, primary, Cheng, Keren, additional, McCarrey, John R., additional, Yadu, Nomesh, additional, Cheeseman, Ian, additional, Bagwell, Alec, additional, Ross, Corinna N., additional, Santana-Toro, Isamar, additional, Yen, Li-Hua, additional, Vargas, Sean, additional, Navara, Christopher S., additional, Hermann, Brian P., additional, and Sasaki, Kotaro, additional

Published
2022
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19. The developmental origin and the specification of the adrenal cortex in humans and cynomolgus monkeys

Author

Cheng, Keren, primary, Seita, Yasunari, additional, Moriwaki, Taku, additional, Noshiro, Kiwamu, additional, Sakata, Yuka, additional, Hwang, Young Sun, additional, Torigoe, Toshihiko, additional, Saitou, Mitinori, additional, Tsuchiya, Hideaki, additional, Iwatani, Chizuru, additional, Hosaka, Masayoshi, additional, Ohkouchi, Toshihiro, additional, Watari, Hidemichi, additional, Umazume, Takeshi, additional, and Sasaki, Kotaro, additional

Published
2022
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20. The embryonic ontogeny of the gonadal somatic cells in mice and monkeys

Author

50765469, 40648424, 50391892, 60546993, Sasaki, Kotaro, Oguchi, Akiko, Cheng, Keren, Murakawa, Yasuhiro, Okamoto, Ikuhiro, Ohta, Hiroshi, Yabuta, Yukihiro, Iwatani, Chizuru, Tsuchiya, Hideaki, Yamamoto, Takuya, Seita, Yasunari, Saitou, Mitinori, 50765469, 40648424, 50391892, 60546993, Sasaki, Kotaro, Oguchi, Akiko, Cheng, Keren, Murakawa, Yasuhiro, Okamoto, Ikuhiro, Ohta, Hiroshi, Yabuta, Yukihiro, Iwatani, Chizuru, Tsuchiya, Hideaki, Yamamoto, Takuya, Seita, Yasunari, and Saitou, Mitinori

Abstract

In the early fetal stage, the gonads are bipotent and only later become the ovary or testis, depending on the genetic sex. Despite many studies examining how sex determination occurs from biopotential gonads, the spatial and temporal organization of bipotential gonads and their progenitors is poorly understood. Here, using lineage tracing in mice, we find that the gonads originate from a T⁺ primitive streak through WT1⁺ posterior intermediate mesoderm and appear to share origins anteriorly with the adrenal glands and posteriorly with the metanephric mesenchyme. Comparative single-cell transcriptomic analyses in mouse and cynomolgus monkey embryos reveal the convergence of the lineage trajectory and genetic programs accompanying the specification of biopotential gonadal progenitor cells. This process involves sustained expression of epithelial genes and upregulation of mesenchymal genes, thereby conferring an epithelial-mesenchymal hybrid state. Our study provides key resources for understanding early gonadogenesis in mice and primates.

Published
2021

23. Chromosomal-scale De novo Genome Assemblies of Cynomolgus Macaque and Common Marmoset

Author

Jayakumar, Vasanthan, primary, Nishimura, Osamu, additional, Kadota, Mitsutaka, additional, Hirose, Naoki, additional, Sano, Hiromi, additional, Murakawa, Yasuhiro, additional, Yamamoto, Yumiko, additional, Nakaya, Masataka, additional, Tsukiyama, Tomoyuki, additional, Seita, Yasunari, additional, Nakamura, Shinichiro, additional, Kawai, Jun, additional, Sasaki, Erika, additional, Ema, Masatsugu, additional, Kuraku, Shigehiro, additional, Kawaji, Hideya, additional, and Sakakibara, Yasubumi, additional

Published
2020
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25. Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein

Author

Seita, Yasunari, primary, Morimura, Toshifumi, additional, Watanabe, Naoki, additional, Iwatani, Chizuru, additional, Tsuchiya, Hideaki, additional, Nakamura, Shinichiro, additional, Suzuki, Toshiharu, additional, Yanagisawa, Daijiro, additional, Tsukiyama, Tomoyuki, additional, Nakaya, Masataka, additional, Okamura, Eiichi, additional, Muto, Masanaga, additional, Ema, Masatsugu, additional, Nishimura, Masaki, additional, and Tooyama, Ikuo, additional

Published
2020
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27. A developmental coordinate of pluripotency among mice, monkeys and humans

Author

Nakamura, Tomonori, Okamoto, Ikuhiro, Sasaki, Kotaro, Yabuta, Yukihiro, Iwatani, Chizuru, Tsuchiya, Hideaki, Seita, Yasunari, Nakamura, Shinichiro, Yamamoto, Takuya, and Saitou, Mitinori

Subjects
Research, Genetic aspects, Embryonic development -- Research, Mammals -- Genetic aspects, Cytological research
Abstract

Author(s): Tomonori Nakamura [1, 2]; Ikuhiro Okamoto [1, 2]; Kotaro Sasaki [1, 2]; Yukihiro Yabuta [1, 2]; Chizuru Iwatani [3]; Hideaki Tsuchiya [3]; Yasunari Seita [3]; Shinichiro Nakamura [3]; Takuya [...], The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of pluripotent stem cells in vitro. To explore the ontogeny of human and primate pluripotency, here we perform comprehensive single-cell RNA sequencing for pre- and post-implantation EPI development in cynomolgus monkeys (Macaca fascicularis). We show that after specification in the blastocysts, EPI from cynomolgus monkeys (cyEPI) undergoes major transcriptome changes on implantation. Thereafter, while generating gastrulating cells, cyEPI stably maintains its transcriptome over a week, retains a unique set of pluripotency genes and acquires properties for neuron differentiation. Human and monkey pluripotent stem cells show the highest similarity to post-implantation late cyEPI, which, despite co-existing with gastrulating cells, bears characteristics of pre-gastrulating mouse EPI and epiblast-like cells in vitro. These findings not only reveal the divergence and coherence of EPI development, but also identify a developmental coordinate of the spectrum of pluripotency among key species, providing a basis for better regulation of human pluripotency in vitro.

Published
2016
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28. Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts

Author

40648424, Matsumoto, Shoma, Porter, Christopher J., Ogasawara, Naomi, Iwatani, Chizuru, Tsuchiya, Hideaki, Seita, Yasunari, Chang, Yu-Wei, Okamoto, Ikuhiro, Saitou, Mitinori, Ema, Masatsugu, Perkins, Theodore J., Stanford, William L., Tanaka, Satoshi, 40648424, Matsumoto, Shoma, Porter, Christopher J., Ogasawara, Naomi, Iwatani, Chizuru, Tsuchiya, Hideaki, Seita, Yasunari, Chang, Yu-Wei, Okamoto, Ikuhiro, Saitou, Mitinori, Ema, Masatsugu, Perkins, Theodore J., Stanford, William L., and Tanaka, Satoshi

Abstract

The placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental development and trophoblast differentiation. In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. Fibroblast growth factor 4 (FGF4) enhanced proliferation of macTSCs, while other exogenous factors were not required to maintain their undifferentiated state. macTSCs showed a trophoblastic gene expression profile and trophoblast-like DNA methylation status and also exhibited differentiation capacity towards invasive trophoblast cells and multinucleated syncytia. In a xenogeneic chimera assay, these stem cells contributed to trophectoderm (TE) development in the chimeric blastocysts. macTSC are the first primate trophoblast cell lines whose proliferation is promoted by FGF4. These cell lines provide a valuable in vitro culture model to analyze the similarities and differences in placental development between human and non-human primates.

Published
2020

30. Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†

Author

Seita, Yasunari, primary, Tsukiyama, Tomoyuki, additional, Azami, Takuya, additional, Kobayashi, Kenichi, additional, Iwatani, Chizuru, additional, Tsuchiya, Hideaki, additional, Nakaya, Masataka, additional, Tanabe, Hideyuki, additional, Hitoshi, Seiji, additional, Miyoshi, Hiroyuki, additional, Nakamura, Shinichiro, additional, Kawauchi, Akihiro, additional, and Ema, Masatsugu, additional

Published
2019
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32. Generation of rat offspring derived from sperm cryopreserved/banked in the National BioResource Project for the rat followed by transportation to another institution

Author

Kashiwazaki, Naomi, Seita, Yasunari, Naoi, Kuniko, Takizawa, Akiko, Kuramoto, Takashi, Ito, Junya, Inomata, Tomo, Serikawa, Tadao, Naomi, Kashiwazaki, Yasunari, Seita, Kuniko, Naoi, Akiko, Takizawa, Takashi, Kuramoto, Junya, Ito, Tomo, Inomata, and Tadao, Serikawa

Subjects
endocrine system, sperm cryopreservation, urogenital system, rat sperm, reproductive and urinary physiology, intrauterine insemination
Abstract

To preserve genetic resources efficiently in rats, sperm cryopreservation is essential. This study aimed to confirm the ability of cryopreserved and transported rat spermatozoa to fertilize through intrauterine insemination. The Komeda miniature rat Ishikawa is a mutant caused by the autosomal recessive mutation mri. The epididymal sperm was frozen with egg yolk medium and banked at the National BioResource Project (NBRP), Kyoto University. The sperm was transported to Azabu University, then thawed at 37℃. The thawed semen was inseminated into the uterine horns of recipients; its motility was around 10%. Seven of 15 inseminated female rats became pregnant and 13 live pups were born. The results indicate that rat spermatozoa cryopreserved at NBRP are capable of restoring genetic resources through intrauterine insemination. We also confirmed the usefulness of assisted reproductive technologies for the rat including sperm cryopreservation and intrauterine insemination.

Published
2009

34. Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body

Author

Seita, Yasunari, primary, Tsukiyama, Tomoyuki, additional, Iwatani, Chizuru, additional, Tsuchiya, Hideaki, additional, Matsushita, Jun, additional, Azami, Takuya, additional, Okahara, Junko, additional, Nakamura, Shinichiro, additional, Hayashi, Yoshitaka, additional, Hitoshi, Seiji, additional, Itoh, Yasushi, additional, Imamura, Takeshi, additional, Nishimura, Masaki, additional, Tooyama, Ikuo, additional, Miyoshi, Hiroyuki, additional, Saitou, Mitinori, additional, Ogasawara, Kazumasa, additional, Sasaki, Erika, additional, and Ema, Masatsugu, additional

Published
2016
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48. Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells.

Author

Dai-tze Wu, Seita, Yasunari, Xia Zhang, Chi-Wei Lu, and Roth, Monica J.

Subjects
*LENTIVIRUS diseases, *INDUCED pluripotent stem cells, *HUMAN embryonic stem cells, *CYTOLOGY, *FIBROBLASTS, *PUROMYCIN, *CELL membranes
Abstract

The identification of stem cells within a mixed population of cells is a major hurdle for stem cell biology-in particular, in the identification of induced pluripotent stem (iPS) cells during the reprogramming process. Based on the selective expression of stem cell surface markers, a method to specifically infect stem cells through antibody-conjugated lentiviral particles has been developed that can deliver both visual markers for live-cell imaging as well as selectable markers to enrich for iPS cells. Antibodies recognizing SSEA4 and CD24 mediated the selective infection of the iPS cells over the parental human fibroblasts, allowing for rapid expansion of these cells by puromycin selection. Adaptation of the vector allows for the selective marking of human embryonic stem (hES) cells for their removal from a population of differentiated cells. This method has the benefit that it not only identifies stem cells, but that specific genes, including positive and negative selection markers, regulatory genes or miRNA can be delivered to the targeted stem cells. The ability to specifically target gene delivery to human pluripotent stem cells has broad applications in tissue engineering and stem cell therapies. [ABSTRACT FROM AUTHOR]

Published
2012
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50. Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells.

Author

Seita Y, Hwang YS, and Sasaki K

Subjects
Male, Humans, Germ Cells, Cell Differentiation, Testis, Induced Pluripotent Stem Cells, Pluripotent Stem Cells
Abstract

There is a scarcity of information regarding the molecular mechanisms underlying human germ cell development due to limitations in obtaining the relevant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would provide critical insight into the etiology of various reproductive conditions and disorders, including infertility.Recently, we reported the in vitro reconstitution of human prospermatogonial development from human-induced pluripotent stem cells through human primordial germ cell (PGC)-like cells (hPGCLCs) using long-term cultured xenogeneic reconstituted testes. Here, we describe a method to generate M-prospermatogonia-like cells (MLCs) and T1-prospermatogonia-like cells (T1LCs), which closely resemble M- and T1-prospermatogonia present in second-trimester human fetal testes in vivo., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2023
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