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1,349 results on '"Sekiguchi H"'

1. Possible Regulation of bFGF Expression by Mast Cells in Osteoarthritis Patients with Obesity: A Cross-Sectional Study

Author

Takata K, Uchida K, Takano S, Mukai M, Inoue G, Sekiguchi H, Aikawa J, Miyagi M, Iwase D, and Takaso M

Subjects
obesity, mast cell, osteoarthritis, bfgf, il13, synovial tissues, Specialties of internal medicine, RC581-951
Abstract

Ken Takata, Kentaro Uchida, Shotaro Takano, Manabu Mukai, Gen Inoue, Hiroyuki Sekiguchi, Jun Aikawa, Masayuki Miyagi, Dai Iwase, Masashi Takaso Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, 252-0374, JapanCorrespondence: Kentaro UchidaDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-Ku Kitasato, Sagamihara City, Kanagawa, 252-0374, JapanTel/Fax +81-042-778-9217Email kuchida@med.kitasato-u.ac.jpPurpose: Obesity is associated with the risk of developing knee osteoarthritis (KOA). Furthermore, synovial basic fibroblast growth factor (bFGF) is linked to the severity of KOA. We previously demonstrated that bFGF and mast cell (MC) marker expression were elevated in the synovial tissues (ST) of KOA patients with obesity. However, it remains unclear whether MCs contribute to bFGF expression and regulation.Patients and Methods: Radiographically diagnosed KOA patients (n=249) were assigned to groups based on the body mass index (BMI) classifications used by the World Health Organization: normal-weight (NW, BMI < 25 kg/m2, n=95), overweight (OW, BMI ≥ 25 and < 30, n=109) and obese (OB, ≥ 30 kg/m2, n=45). BFGF expression in the ST was examined using quantitative polymerase chain reaction and compared across the BMI groups. Additionally, BFGF and interleukin (IL) 13 expression were examined in freshly extracted MC-rich (THY-1-, CD3-, CD14-, and CD19-) and MC-poor (THY-1+, CD3+, CD14+, or CD19+) fractions from ST. Moreover, regulation of BFGF expression by IL-13 was studied in CD14-negative (fibroblast-rich) and CD14-positive (M&phis;-rich) and cells in culture.Results: BFGF expression was significantly higher in OB than in NW patients. Furthermore, although IL13 was significantly higher in the MC-rich than the MC-poor fraction, BFGF expression was comparable. Recombinant human IL-13 stimulated expression of BFGF in synovial fibroblast cells.Conclusion: BFGF expression is higher in the ST of KOA patients with obesity. Increased numbers of MCs may contribute to the elevated BFGF expression through IL-13 in KOA patients with obesity.Keywords: obesity, mast cell, osteoarthritis, BFGF, IL13, synovial tissues

Published
2021

2. Increase in Tryptase and Its Role in the Synovial Membrane of Overweight and Obese Patients with Osteoarthritis of the Knee

Author

Takata K, Uchida K, Mukai M, Takano S, Aikawa J, Iwase D, Sekiguchi H, Miyagi M, Inoue G, and Takaso M

Subjects
obese, mast cell, osteoarthritis, tryptase, synovial membrane, Specialties of internal medicine, RC581-951
Abstract

Ken Takata,1 Kentaro Uchida,1,2 Manabu Mukai,1 Shotaro Takano,1 Jun Aikawa,1 Dai Iwase,1 Hiroyuki Sekiguchi,2 Masayuki Miyagi,1 Gen Inoue,1 Masashi Takaso1 1Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa 252-0374, Japan; 2Shonan University of Medical Sciences Research Institute, Chigasaki City, Kanagawa 253-0083, JapanCorrespondence: Kentaro Uchida Tel/Fax +81-42-778-9217Email kuchida@med.kitasato-u.ac.jpPurpose: The mechanisms governing evidence that obesity is a risk factor for osteoarthritis (OA) are not well understood. We previously reported an increase in mast cell (MC) marker expression in the osteoarthritic synovial membrane (SM) of patients with obesity. We hypothesized that an enzyme produced by MC, β-tryptase, may be increased in the SM of obese patients with knee OA and contribute to synovial inflammation. This study investigated the expression of the β-tryptase encoding gene, TPSB2, in the SM of obese patients with knee OA and β-tryptase-mediated regulation of IL-1β in synovial cells.Patients and Methods: A total of 216 patients radiographically diagnosed with knee OA were grouped according to the World Health Organization’s body mass index classifications: normal weight (NW; < 25 kg/m2), overweight (OW; 25– 29.99 kg/m2) and obese (OB; ≥ 30 kg/m2). Quantitative polymerase chain reaction was conducted to examine TPSB2 expression in the SM among the three groups. We also examined TPSB2 and IL1B expression in MC-rich (CD3-CD14-CD19-CD90-) and MC-poor (CD3+, CD14+, CD19+, or CD90+) fractions freshly isolated from synovial tissue. Further, the effect of β-tryptase on IL1B expression was investigated in cultured CD14-positive (macrophage-rich fraction) and CD14-negative (fibroblast-rich fraction) cells.Results: There was significantly elevated TPSB2 expression in the OW and OB groups compared to the NW group. The MC-rich fraction had significantly higher levels of TPSB2, CD117 and CD203c than the MC-poor fraction. Recombinant human β-tryptase stimulated IL1B expression in both the synovial fibroblast and macrophage fractions.Conclusion: Obese patients with knee OA showed elevated TPSB2 expression in the SM. Tryptase may play a role in synovial inflammation in obese patients with OA.Keywords: obese, mast cell, osteoarthritis, tryptase, synovial membrane

Published
2020

3. Osteoarthritis patients with high haemoglobin A1c have increased Toll-like receptor 4 and matrix metalloprotease-13 expression in the synovium

Author

Murata K, Uchida K, Takano S, Shoji S, Iwase D, Inoue G, Aikawa J, Yokozeki Y, Sekiguchi H, and Takaso M

Subjects
osteoarthritis, diabetes mellitus, TLR4, MMP13, Specialties of internal medicine, RC581-951
Abstract

Kosuke Murata,1 Kentaro Uchida,1 Shotaro Takano,1 Shintaro Shoji,1 Dai Iwase,1 Gen Inoue,1 Jun Aikawa,1 Yuji Yokozeki,1 Hiroyuki Sekiguchi,2 Masashi Takaso11Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa 252-0374, Japan; 2Shonan University of Medical Sciences Research Institute, Chigasaki City, Kanagawa 253-0083, JapanPurpose: While research has identified diabetes mellitus (DM) as a risk factor for knee osteoarthritis (KOA), the underlying mechanisms are not fully understood. Studies suggest that Toll-like receptor 4 (TLR4) expression is elevated in osteoarthritic lesions of OA patients and in target tissues of insulin resistance such as adipose tissue and skeletal muscle in patients with DM. TLR4 is associated with inflammation and catabolic response via regulation of matrix metalloproteases (MMPs). We hypothesized that TLR4 and MMP expression may be increased in the synovial tissue (SYN) of KOA patients with diabetic pathology. We therefore investigated TLR and MMP expression in the SYN of KOA patients with and without high haemoglobin A1c concentrations.Patients and methods: A total of 171 patients radiographically diagnosed with KOA were grouped based on their HbA1c concentration (HbA1c ≥6.5 and HbA1c

Published
2019

4. Increase in mast cell marker expression in the synovium of obese patients with osteoarthritis of the knee

Author

Uchida K, Takano S, Inoue G, Iwase D, Aikawa J, Takata K, Tazawa R, Kawakubo A, Sekiguchi H, and Takaso M

Subjects
Mast cells, obese, synovium, osteoarthritis, Specialties of internal medicine, RC581-951
Abstract

Kentaro Uchida,1 Shotaro Takano,1 Gen Inoue,1 Dai Iwase,1 Jun Aikawa,1 Ken Takata,1 Ryo Tazawa,1 Ayumu Kawakubo,1 Hiroyuki Sekiguchi,2 Masashi Takaso11Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa 252–0374, Japan; 2Shonan University of Medical Sciences Research Institute, Chigasaki City, Kanagawa 253–0083, JapanPurpose: While research suggests that obesity is a risk factor for knee osteoarthritis (KOA), the mechanisms are not fully understood. Mast cell (MC) numbers are increased in the osteoarthritic synovium and in the adipose tissue of obese individuals. We hypothesized that MC numbers are increased in the synovium of obese KOA patients. This study investigated MC marker and MC-generated cytokine/growth factor expression in the synovium of obese KOA patients.Patients and methods: Patients radiographically diagnosed with KOA (male: 38, female: 132) were allocated to three groups based on their body mass index (BMI): normal (

Published
2019

6. Ferroic Berry Curvature Dipole in a Topological Crystalline Insulator at Room Temperature

Author

Nishijima, T., Watanabe, T., Sekiguchi, H., Ando, Y., Shigematsu, E., Ohshima, R., Kuroda, S., and Shiraishi, M.

Subjects
Condensed Matter - Mesoscale and Nanoscale Physics, Condensed Matter - Materials Science
Abstract

The physics related to Berry curvature is now a central research topic in condensed matter physics. The Berry curvature dipole (BCD) is a significant and intriguing condensed matter phenomenon that involves inversion symmetry breaking. However, the creation and controllability of BCDs have so far been limited to far below room temperature (RT) and non-volatile (i.e., ferroic) BCDs have not yet been discovered, hindering further progress in topological physics. In this work, we demonstrate a switchable and non-volatile BCD effect at RT in a topological crystalline insulator, Pb1-xSnxTe (PST), which is attributed to ferroic distortion. Surprisingly, the magnitude of the ferroic BCD is several orders of magnitude greater than that of the non-ferroic BCDs that appear, for example, in transition metal dichalcogenides. The discovery of this ferroic and extraordinarily large BCD in PST could pave the way for further progress in topological material science and the engineering of novel topological devices., Comment: 23 pages, 3 figures

Published
2023
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10. Precise phase retrieval for propagation-based images using discrete mathematics

Author

Pollock, J. A., Morgan, K. S., Croton, L. C. P., Croughan, M. K., Ruben, G., Yagi, N., Sekiguchi, H., and Kitchen, M. J.

Subjects
Electrical Engineering and Systems Science - Image and Video Processing, Physics - Medical Physics
Abstract

The ill-posed problem of phase retrieval in optics, using one or more intensity measurements, has a multitude of applications using electromagnetic or matter waves. Many phase retrieval algorithms are computed on pixel arrays using discrete Fourier transforms due to their high computational efficiency. However, the mathematics underpinning these algorithms is typically formulated using continuous mathematics, which can result in a loss in spatial resolution in the reconstructed images. Herein we investigate how phase retrieval algorithms for propagation-based phase-contrast X-ray imaging can be rederived using discrete mathematics and result in more precise retrieval for single- and multi-material objects and for spectral image decomposition. We validate this theory through experimental measurements of spatial resolution using computed tomography (CT) reconstructions of plastic phantoms and biological tissue, using detectors with a range of imaging system point spread functions (PSFs). We demonstrate that if the PSF substantially suppresses high spatial frequencies, the potential improvement from utilising the discrete derivation is limited. However, with detectors characterised by a single pixel PSF (e.g. direct, photon-counting X-ray detectors), a significant improvement in spatial resolution can be obtained, demonstrated here at up to 17%.

Published
2021

31. The 2019 American College of Rheumatology/European League Against Rheumatism classification criteria for IgG4-related disease

Author

Wallace, Zachary S, Naden, Ray P, Chari, Suresh, Choi, Hyon K, Della-Torre, Emanuel, Dicaire, Jean-Francois, Hart, Phillip A, Inoue, Dai, Kawano, Mitsuhiro, Khosroshahi, Arezou, Lanzillotta, Marco, Okazaki, Kazuichi, Perugino, Cory A, Sharma, Amita, Saeki, Takako, Schleinitz, Nicolas, Takahashi, Naoki, Umehara, Hisanori, Zen, Yoh, Stone, Collaborators: Akamizu T, John H., Akiyama, M, Barra, L, Bateman, A, Blockmans, D, Brito-Zeron, P, Campochiaro, C, Carruthers, M, Chiba, T, Cornell, L, Culver, E, Darabian, S, Deshpande, V, Dong, L, Ebbo, M, Fernández-Codina, A, Ferry, Ja, Fragkoulis, G, Frost, F, Frulloni, Luca, Hernandez-Molina, G, Ji, H, Keat, K, Kamisawa, T, Kawa, S, Kobayashi, H, Kodama, Y, Kubo, S, Kubota, K, Leng, H, Lerch, Mm, Liu, Y, Liu, Z, Löhr, M, Martin-Nares, E, Martinez-Valle, F, Marvisi, C, Masaki, Y, Matsui, S, Mizushima, I, Nakamura, S, Nordeide, J, Notohara, K, Paira, S, Popovic, J, Ramos-Casals, M, Rosenbaum, J, Ryu, J, Sato, Y, Sekiguchi, H, Sokol, Ev, Stone, Jr, Sun, W, Takahashi, H, Takahira, M, Tanaka, Y, Vaglio, A, Villamil, A, Wada, Y, Webster, G, Yamada, K, Yamamoto, M, Yi, J, Yi, Y, Zamboni, G, Zhang, W., Wallace, Z, Naden, Rp, Chari, S, Choi, Hk, DELLA TORRE, E, Dicaire, Jf, Hart, Pa, Inoue, D, Kawano, M, Khosroshahi, A, Lanzillotta, M, Okazaki, K, Perugino, Ca, Sharma, A, Saeki, T, Schleinitz, N, Takahashi, N, Umehara, H, Zen, Y, Stone, Jh, and Members of the ACR/EULAR IgG4-RD Classification Criteria Working, Group.

Subjects
rheumatoid arthritis, Adult, Male, 0301 basic medicine, medicine.medical_specialty, Immunology, Sensitivity and Specificity, General Biochemistry, Genetics and Molecular Biology, Diagnosis, Differential, 03 medical and health sciences, 0302 clinical medicine, Rheumatology, Internal medicine, parasitic diseases, Epidemiology, Humans, Immunology and Allergy, Medicine, Pathological, Sjøgren's syndrome, Aged, 030203 arthritis & rheumatology, business.industry, Middle Aged, inflammation, medicine.disease, Test (assessment), 030104 developmental biology, Rheumatoid arthritis, Radiological weapon, Female, Immunoglobulin G4-Related Disease, business, Rheumatism, Decision analysis
Abstract

IgG4-related disease (IgG4-RD) can cause fibroinflammatory lesions in nearly any organ. Correlation among clinical, serological, radiological and pathological data is required for diagnosis. This work was undertaken to develop and validate an international set of classification criteria for IgG4-RD. An international multispecialty group of 86 physicians was assembled by the American College of Rheumatology (ACR) and the European League Against Rheumatism (EULAR). Investigators used consensus exercises; existing literature; derivation and validation cohorts of 1879 subjects (1086 cases, 793 mimickers); and multicriterion decision analysis to identify, weight and test potential classification criteria. Two independent validation cohorts were included. A three-step classification process was developed. First, it must be demonstrated that a potential IgG4-RD case has involvement of at least one of 11 possible organs in a manner consistent with IgG4-RD. Second, exclusion criteria consisting of a total of 32 clinical, serological, radiological and pathological items must be applied; the presence of any of these criteria eliminates the patient from IgG4-RD classification. Third, eight weighted inclusion criteria domains, addressing clinical findings, serological results, radiological assessments and pathological interpretations, are applied. In the first validation cohort, a threshold of 20 points had a specificity of 99.2% (95% CI 97.2% to 99.8%) and a sensitivity of 85.5% (95% CI 81.9% to 88.5%). In the second, the specificity was 97.8% (95% CI 93.7% to 99.2%) and the sensitivity was 82.0% (95% CI 77.0% to 86.1%). The criteria were shown to have robust test characteristics over a wide range of thresholds. ACR/EULAR classification criteria for IgG4-RD have been developed and validated in a large cohort of patients. These criteria demonstrate excellent test performance and should contribute substantially to future clinical, epidemiological and basic science investigations.

Published
2019

45. Increase of operating current of the LHD helical coils by upgrading the cooling system

Author

Imagawa, S., Obana, T., Hamaguchi, S., Yanagi, N., Mito, T., Moriuchi, S., Sekiguchi, H., Ooba, K., Okamura, T., and Komori, A.

Subjects
Electric coils -- Design and construction, Liquid helium -- Usage, Superconducting magnets -- Heating, cooling and ventilation, Business, Electronics, Electronics and electrical industries
Published
2010

47. Performance tests of the subcooling system for the LHD helical coils

Author

Obana, T., Imagawa, S., Hamaguchi, S., Yanagi, N., Moriuchi, S., Sekiguchi, H., Oba, K., and Mito, T.

Subjects
Electric coils -- Heating, cooling and ventilation, Helium -- Thermal properties, Superconductivity -- Analysis, Business, Electronics, Electronics and electrical industries
Abstract

Performance tests are carried out on the subcooling system for the Large Helical Device (LHD) helical coils. The studies have shown that the coil temperature is around 3.6 K under a steady heat load of 100 W from outside of the coil case in the system, which has maintained the above performance.

Published
2008

48. Results of the excitation test of the LHD helical coils cooled by subcooled helium

Author

Imagawa, S., Obana, T., Hamaguchi, S., Yanagi, N., Mito, T., Moriuchi, S., Sekiguchi, H., Ooba, K., Okamura, T., Komori, A., and Motojima, O.

Subjects
Helium -- Thermal properties, Superconductors -- Usage, Business, Electronics, Electronics and electrical industries
Abstract

The cryogenic stability of the Large Helical Device (LHD) helical coils is improved by installing an additional cooler like subcooled helium at 3.0 K. The temperature rise by excitation is analyzed as it is required for maintaining low temperatures for better heat transfer.

Published
2008

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