Search

Your search keyword '"Selaković, Srećko"' showing total 18 results
Start Over Author "Selaković, Srećko"
18 results on '"Selaković, Srećko"'

1. Thermal Vision Examination of Vasoconstriction and Vasodilatation of Blood Vessels under the Influence of Local Anesthetic Solutions

Author

Đukić Igor, Selaković Srećko, Bubalo Marija, Selaković Jovana, and Tatić Zoran

Subjects
las, adrenaline, noradrenaline, thermal camera, temperature, Veterinary medicine, SF600-1100
Abstract

A quality local anesthesia is one of the imperatives of performing dental interventions, and especially oral-surgical interventions, where the success of the oral-surgical intervention is seen through the success of local anesthesia. There is a high number of different local anesthetic solutions (LAS) on the market, holding different levels of vasoconstrictors which are attributed with causing numerous complications during the local anesthesia. The aim of this research is to point out the temperature changes caused by different LAS, depending on the strength and the concentration of LAS, using thermal vision camera. Testing was performed on 70 experimental Wistar rats, aged 4.5 months, with an average weight of 250 grams, male, and divided into seven groups of 10 animals each, depending on the applied anesthetic. After injecting the anesthetic solution into the haunch area, a stopwatch was turned on and control measurements were conducted for a period of two hours. All footage from the thermal camera was later transferred to a computer unit. Occurrence of multiple temperatures in the rats’ examined regions, compared to the control region, was noticed only when applying 2% pure lidocaine (without vasoconstrictors). The highest temperature drop, compared to the control region, was noticed with 2% mepivacaine with adrenaline (1 : 100 000), and 2% mepivacaine with noradrenaline (1 : 100 000), fi ve minutes after applying the local anesthetic. In conclusion: vasoconstricting effects are most expressed in the fi fth minute after applying LAS, and there are minimal differences between the effects of adrenaline and noradrenaline, in the examined combinations together with LAS.

Published
2018
Full Text
View/download PDF

2. Medico-legal expertise of pain in dental trauma

Author

Selaković Srećko, Mirković Siniša, Bajkin Branislav, Šarčev Ivan, Tadić Ana, and Selaković Jovana

Subjects
dentistry, forensic, pain measurement, trauma severity indices, therapeutics, ethics, dental, Medicine (General), R5-920
Abstract

nema

Published
2016
Full Text
View/download PDF

3. Comparison of efficacy of local hemostatic modalities in anticoagulated patients undergoing tooth extractions

Author

Bajkin Branislav V., Selaković Srećko D., Mirković Siniša M., Šarčev Ivan N., Tadić Ana J., and Milekić Bojana R.

Subjects
tooth extraction, antiacoagulants, administration+ oral, drug therapy, hemostasis, Medicine (General), R5-920
Abstract

Background/Aim. Patients receiving long-term oral anticoagulant therapy pose a clinical challenge during invasive dental procedures. The goal of this study was to compare different local hemostatic modalities after tooth extraction in patients receiving chronic Vitamin-K antagonist therapy. Methods. Totally 90 patients with International Normalized Ratio (INR) ≤ 3.0 requiring simple extraction of one or two teeth were randomized into three groups, 30 patients in each group. The patients with the mean INR value of 2.35 ± 0.37, in whom extraction wound was sutured comprised the group A. In the group B with the mean INR of 2.43 ± 0.4, local hemostasis was achieved by placing absorbable gelatin sponges into the wound without suturing. The group C consisted of the patients with the mean INR of 2.36 ± 0.34 in whom neither gelatin sponge nor suturing were used for providing local hemostasis. Bleeding was registered as an event if other than initial hemostatic measure was needed or additional oral surgeon intervention required. Results. The obtainded results show that 1 (3.3%) patient in the group A, 2 (6.7%) patients in the groups B and C manifested post-extraction bleeding. All cases of hemorrhage were easily solved with local hemostatic measures and all, except one case, were registered in the first two hours after the procedure until the dismissal. A difference between the groups was not statistically significant (χ2 = .42, p > 0.05). Conclusion. In therapeutically anticoagulated patients tooth extractions can be safely performed without altering the dose of anticoagulant medication if efficient local hemostasis is provided. In most cases, in patients with INR ≤ 3.0 after extraction of one or two teeth postoperative bleeding can be controlled with local pressure, without any additional local hemostatic measures.

Published
2014
Full Text
View/download PDF

4. Mechanical resistance of restored teeth with extensive crown damage

Author

Šarčev Ivan, Petronijević Branislava, Marković Dubravka, and Selaković Srećko

Subjects
core build-up, compressive strength test, composite resin, dental amalgam, glass ionomer cements, Dentistry, RK1-715
Abstract

Introduction. Extensively damaged teeth can be restored by different core build-up materials. The aim of this study was to examine the mechanical properties of the restored maxillary premolars with composite resin, dental amalgam and glass ionomer cement (GIC) using compressive strength test. Materials and methods. Forty extracted intact human maxillary premolars were used in this study. Artificial defect in dentin was prepared using diamond bur up to the half of the anatomic crown of the tooth. After core build-up procedure, each tooth specimen was mounted in auto polymerizing acrylic resin blocks 2 mm below cement enamel junction and they were kept in distilled water at 37°C one day before testing. Then, they were placed in specially adapted devices at the angle of 183° to the longitudinal axis and subjected to a controlled load of 1 mm per minute. Results. Results showed that the best mechanical properties had samples restored with resin composite (492.5 N), then with amalgam (341.2 N) and glass ionomer cement (171.8 N). Comparing the fracture force using ANOVA, there was statistic significance between these groups (p

Published
2009
Full Text
View/download PDF

5. Influence of different types of surgical suture material on the intensity of tissue reaction in oral cavity

Author

Mirković Siniša M., Džambas Ljubiša D., and Selaković Srećko Đ.

Subjects
suture material, tissue reaction, oral cavity, Science (General), Q1-390
Abstract

Throughout the history the most diverse suture material have been used for closing and suturing surgical wounds. The four basic features of suture material are described: knot safety, stretch capacity, tissue reactivity and wound safety. Tissue reaction, even the minimum one, which develops during the first to seven days after applying the suture in the tissue. The aim of this study was to investigate influence of a monofilament suture material (nylon) on the intensity of local tissue reaction in experimental conditions, and to compare it with the multifilament suture used in the routine practice of oral surgery (silk). This investigation is a prospective experimental study carried out on Wistar rats. The experiment included 30 animals, in which Black Silk (thickness 4-1) and Nylon (thickness 4-0) were applied in the upper and lower jaw, respectively. To monitor tissue reaction on different suture materials the following parameters were used: coagulum formation, presence of polymorphonuclear leukocytes, presence of macrophages and granuloma, formation of epithelial bridge and connective tissue, collagen synthesis, granulomatous tissue formation and presence of fibrous tissue. After comparing parameters for the intensity of tissue reaction to the investigate suture materials by suturing the oral mucosa, certain advantage could be given to the monofilament suture materials.

Published
2008
Full Text
View/download PDF

6. Uticaj preparata koncentrovanih faktora rasta na regeneratorne i reparatorne procese u postekstrakcionim ranama

Author

Mirković, Siniša, Selaković, Srećko, Čolić, Snježana, Puškar, Tatjana, Šarčev, Ivan, Bajkin, Branislav, Tadić, Ana, Mirković, Siniša, Selaković, Srećko, Čolić, Snježana, Puškar, Tatjana, Šarčev, Ivan, Bajkin, Branislav, and Tadić, Ana

Abstract

Ekstrakcija zuba je intervencija prilikom koje se zub vadi iz svog ležišta u zubnoj alveoli. Rana koja nastaje kao posledica ove intervncije je takvog oblika da njene ivice nije moguće približiti jednu drugoj , te zarasta per secundam intentionem. Ekstrakcija zuba ima za posledicu mnogobrojne promene na tvrdim i mekim tkivima alveolarnog nastavka u periodu od nekoliko meseci do godinu dana nakon intervencije. Savremena stomatologija se i dalje intenzivno bavi proučavanjem procesa zarastanja ekstrakcione rane iz potrebe da se što bolje razumeju promene u tkivu koje nastaju po gubitku zuba da bi se mogle prevenirati i/ili usmeriti tako da se omogući kasnija lakša protetska rehabilitacija pacijenata. Mnoge studije su pokazale efikasnost faktora rasta u tokom procesa zaceljivanja tkiva.Opisan je veliki broj tehnika za pripremu autolognih krvnih preparata koji sadrže faktore rasta, ali su njihova praktična primena i efikasnost su dalje nejasni zato što svaka od ovih metoda dovodi do izrade različitog produkta sa različitom biologijom i potencijalnim indikacijama za upotrebu. Ekstrakcije mandibularnog trećeg molara spadaju u jednu od najčešćih intervencija sa kojom se u svom radu svakodnevno sreću oralni i maksilofacijalni hirurzi. Ova hirurška procedura je povezana za postoperativnim efektima koji u velikoj meri utiču na kvalitet života pacijenta kao što su bol, trizmus, edem, infekcija i alveolitis. U literaturi postoje dokazi da aplikacija nekog od autolognog krvnog preparata sa visokim sadržajem faktora rasta u određenoj meri može da poboljša proces zarastanja tkiva i da umanji neželjene propratne pojave hirurške intervencije nakon ekstrakcije mandibularnog trećeg molara. Cilj ovog rada je bio da utvrdimo da li primena autolognih krvnih preparata sa koncentrovanim faktorima rasta ubrzava stvaranje koštanog tkiva u ekstrakcionoj rani, kao i da li utiče na učestalost pojave alveolitisa i pojavu aproksimalinih parodontalnih džepova na susednim zubima nakon hirurške ekstr, Tooth extraction is an intervention during which a tooth is removed from its socket. A wound that remains after this is of specific size and shape and it heals per secundam intentionem. Where once was a tooth, in following months and years, a large number of changes in composition of hard and soft tissues occure. Haeling of extraction wound in still in focus of contemporary dentistry, since it is imperative to understand all tissue changes in order to prevent and/or gide them and enable prosthodontic rehabilitation of the patient. Many studies confirm a benefitial effect of growth factors douring wound healing. A large number of techniques is developed to prepare autologous blood concentrates containing growth factors, like platelet-rich fibrin (PRF) , but their aplicability and efficancy are still unclear because each of these methods results in product with different biology and physical characteristics, as well as different potential indications. Third mandibular molar extraction is one of the most frequent interventions that oral and maxillofacial surgeon face in their everyday clinical practice. This procedure is usually followed by postoperative effects affecting such as pain, trismus, edema, infection and alveolitis. In contemporary literature there is enough evidence to suport beneficial role of autologous blood preparations in wound healing, and some authors even sugest that they can reduce incidence of postextraction complications afther third molar surgery. The aim of this study was to determin weather concentrated growth factors have beneficial effect on bone healing after tooth extraction, as well as their effect on the incidence of alveolitis and do they reduce pocket depth on distal side of adjacent tooth. This study was conducted as prospective clinical split-mouth designed study. 30 patients with both mandibular third molars indicated for the extraction, in similar position, were included in the study. On the same day surgical removal of both mandib

Published
2019

7. Biokompatibilnost i marginalna adaptacija mineral-trioksid agregata, trikalcijum-silikatnog cementa i amalgama kao materijala za retrogradno zatvaranje kanala korena zuba

Author

Bajkin, Branislav, Mirković, Siniša, Petrović, Ljubomir, Brković, Božidar, Petrović, Bojan, Selaković, Srećko, Jovanović, Lena, Bajkin, Branislav, Mirković, Siniša, Petrović, Ljubomir, Brković, Božidar, Petrović, Bojan, Selaković, Srećko, and Jovanović, Lena

Abstract

Već izvesno vreme u stručnoj literaturi vodi se rasprava o biokompatibilnosti materijala koji se koriste u periapikalnoj hirurgiji. Pored biokompatibilnosti, od materijala za retrogradnu opturaciju kanala korena zuba se očekuje da spreči prodor bakterija iz kanalnog sistema u okolna tkiva. Kvalitetno rubno zaptivanje, odnosno adekvatna marginalna adaptacija su među najznačajnijim faktorima za dugoročan uspeh tretmana. Cilj ovog istraživanja je bio ispitati biokompatibilnost i utvrditi da li postoji razlika u biokompatibilnosti između mineral-trioksid agregata, trikalcijum-silikatnog cementa i amalgama na osnovu tri standadna testa citotoksičnosti, kao i utvrditi marginalnu adaptaciju ispitivanih materijala za retrogradno zatvaranje kanala korena zuba. Materijali i metode: Eksperimentalni deo istraživanja je podeljen na dva dela. U prvom delu istraživanja je vršeno ispitivanje biokompatibilnosti materijala, dok je u drugom delu vršeno ispitivanje marginalne adaptacije na osnovu mikrofotografija dobijenih skening elektronskim mikroskopom. Ispitivanje biokompatibilnosti je izvršeno na dve ćelijske linije, liniji mišjih fibroblasta (L929) i liniji humanih fibroblasta (MRC-5). U eksperimentima su korišćene samo žive (vijabilne) ćelije. Broj ćelija i njihova vijabilnost je određena testom odbacivanja boje sa 0,1% tripan plavim. Vijabilnost ćelija korišćenih u eksperimentu je bila veća od 90%. Biokompatibilnost sve tri vrste materijala je ispitana na osnovu standardnih testova biokompatibilnosti: DET test, MTT test, Agar difuzioni test. Ispitivanje marginalne adaptacije je sprovedeno na 90 ekstrahovanih jednokorenih zuba interkaninog sektora gornje vilice sa intaktnom pulpom, završenim rastom korena, bez frakture i resorpcije korena zuba. Izvršena je endodontska obrada svih zuba i nakon toga su zubi ostavljeni u vlažnoj sredini na 48h, da bi se sprečile frakture prilikom sečenja. Nakon mehaničko medikamentozne obrade i opturacije kanala korena zuba je vršena resekcija vrha, Biocompatibility of materials, deployed in periapical surgery, has been a subject of debate in referential literature for some time now. Apart from biocompabillity, root end filling materials are expected to prevent bacteria from entering the surrounding tissue from canal system. The most important factors for successful long term treatment include marginal seal, i.e. adequate marginal adaptation. The aim of this research was to examine biocompatibility and establish the potential difference in biocompatibility between mineral trioxide aggregate, tricalcium silicate cement and dental amalgam according to three cytotoxicity tests, but also to corroborate marginal adaptation of the materials in question for retrograde seal of a root canal. Materials and methods: The experimental part of the research is divided in two parts. In the first part of the research biocompatibility of the materials was examined, while the examination of the marginal adaptation based on the micro images from scanning electron microscope was conducted in the second part. The examination of biocompatibility was executed on two cell lines, mouse fibroblast cell line (L929) and human cell line (MRC-5).Biocompatibility of all three types of material was examined based on three standard biocompatibility tests: DET test, MTT test, Agar diffusion test.The examination of marginal adaptation was carried out on 90 single-rooted tooth extractied human teeth of the intercanine sector of maxilla with intact pulp, mature apices, without root fractures or resorption. All teeth were endodontically treated. After the extirpation, irrigation and opturation, the resection of 3mm of root apex and retrograde preparation with ultrasonic instruments up to 3 mm depth inside the canal was done. Teeth were divided in three groups alternately. The First group include teeth which apical cavitation was filled with amalgam, in the Second group apical cavitation was filled with MTA, and in the Third group with tricalcium sil

Published
2019

8. Hyaluronic acid treatment outcome on the post-extraction wound healing in patients with poorly controlled type 2 diabetes: A randomized controlled split-mouth study.

Author

Marin, Saša, Popović-Pejičić, Snježana, Radošević-Carić, Bojana, Trtić, Nataša, Tatić, Zoran, and Selaković, Srećko

Subjects
WOUND healing, HYALURONIC acid, TYPE 2 diabetes, TREATMENT effectiveness, MANDIBLE
Abstract

Background: Hyaluronic acid is widely used in the medical field. However, there is a lack of research about its effect on patients with certain risks, such as compromised wound healing commonly found in patients with poorly controlled type 2 diabetes. The aim of this study is to investigate the efficacy of hyaluronic acid on the post-extraction wound healing and pain in patients with poorly controlled type 2 diabetes. Material and Methods: The randomized controlled split-mouth study was designed, which included 30 patients with poorly controlled type 2 diabetes with a bilaterally same teeth in the lower jaw for extraction. The sockets treated with 0.8% hyaluronic acid represented the study group, while the sockets where hyaluronic acid was not applied represented the control group. Wound closure rate (WCR), clinical scores in wound healing scale (WHS) and pain intensity in Visual analogue scale (VAS) were recorded. Patients were followed up on 5th, 10th, 15th, 20th, 25th day after tooth extraction. Results: The results showed a higher WCR at the extraction site where hyaluronic acid was applied. Also, statistically significant difference was found (p < 0.001). In regards to WHS, the sockets treated with hyaluronic acid showed better healing, especially on day 10 (p=0.006) and day 15 (p=0.021). However, there were no statistically significant differences in VAS scores between groups. Conclusions: Hyaluronic acid placed in post-extraction socket in patients with poorly controlled diabetes may improve wound healing, especially in the first days after application. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

15. Biokompatibilnost, citotoksičnost i osteoinduktivnost različitih koštanih zamenika na matičnim ćelijama pulpe mlečnih zuba

Author

Bajkin, Branislav, Petrović, Bojan, Čapo, Ivan, Savić, Slavica, Selaković, Srećko, and Gušić, Ivana

Subjects
Tissue Engineering, Mesenchymal Stem Cells, Tissue Scaffolds, Biocompatible Materials, Dental Pulp, Tooth, Deciduous, Bone Substitutes, Osteoblasts, Dentin, tkivni inženjering, mezenhimalne stem ćelije, nosači tkiva, biokompatibilni materijali, zubna pulpa, mlečni zubi, zamene za kosti, osteoblasti, dentin
Abstract

Uvod: Tkivni inženjering je multidisciplinarno polje koje ima za cilj da obnovi, održi ili poboljša funkcije tkiva koja su oštećena ili su izgubljena usled različitih patoloških stanja, bilo razvojem adekvatnih bioloških zamena ili rekonstrukcijom tkiva. Za uspeh u inženjeringu tkiva veoma su važni sledeći faktori: izbor izvora matičnih ćelija, strategije koje se koriste za izolovanje i pasažiranje specifičnih ćelija, izbor biomaterijala koji će se koristiti kao skafold (nosač) i povezanost među njima. Skafoldi mogu biti različitog porekla, pa tako imamo autografte, alografte, ksenografte i aloplastične materijale. Biomehanički sistem kosti je složen tako da idealan skafold treba da ispunjava sledeće uslove: biokompatibilnost, osteokonduktivnost, osteoinduktivnost, sposobnost angiogeneze. Jedan od najperspektivnijih izvora matičnih ćelija za rekonstrukciju koštanih oštećenja jeste zubna pulpa. Matične ćelije zubne pulpe se jednostavno izoluju kako iz mlečnih (SHED) tako i stalnih zuba (DPSC), u in vitro uslovima se lako umožavaju i za nadoknadu oštećenja kostiju se mogu koristiti u kombinaciji sa ili bez skafolda. Ove ćelije su izuzetno proliferativne i sposobne za osteogeno, dentinogeno, adipogeno, hondrogeno, i neurogeno diferenciranje. Cilj ove disertacije je bila izolacija i karakterizacija mezenhimalnih matičnih ćelija zubne pulpe poreklom iz humanih mlečnih zuba koji se nalaze u svojoj fiziološkoj smeni, i potom određivanje biokompatibilnosti ispitivanih biomaterijala (Bio-oss® (Geistlich, Switzerland, dentinskih opiljaka i SBA-16/HA ), i evaluacija ćelijske vijabilnosti nakon kontakta sa njima. Nakon toga je cilj bio diferencirati i imunohistološki potvrditi osteoblastičnu transformaciju mezenhimalnih matičnih ćelija zubne pulpe gajenih u osteogenom medijumu, koje su kasnije korišćene u ispitivanju osteoinduktivnog potencijala različitih koštanih zamenika. Materijal i metode: Eksperimentalni deo istraživanja je podeljen na pet delova. U prvom delu istraživanja je vršena izolacija i karakterizacija SHED na protočnom citometru, u drugom delu je vršena sinteza i karakterizacija SBA-16/HA materijala, u trećem delu eksperimenta je vršeno ispitivanje biokompatibilnosti tri materijala na osnovu standardnih testova biokompatibilnosti: DET testa, MTT testa. U četvrtom delu vršena je diferencijacija i ispitivanje osteogenog kapaciteta SHED. Potvrda dobijenih osteoblasta je vršena alizarin red testom i imunofluorescentnim bojenjem sledećih primatnih antitela: osteopontin, SOX2, Ki-67 i Coll-1. U poslednjem, petom delu eksperimentalne studije određivana je ekspresija osteogenih markera u SHED tretiranim ispitivanim materijalima. Najpre je izolovana iRNK iz ćelija, potom je reakcijom reverzne transkripcije prevedena u iDNK i na kraju je kvanitifikovana u Real time PCR analizi. U analizi su korišeni RUNX2 i ALPL geni. Rezultati: Izolovanjem SHED dobili smo ćelije koje imaju visok proliferativni potencijal i pozitivno eksprimiraju markere matičnih ćelija na protočnom citometru, čime ispunjavaju neophodne kriterijume da budu definisane kao humane mezenhimalne stem ćelije. Na testovima biokompatibilnosti, MTT i DET, novosintetisani SBA-16/HA materijal i kontrolni Bio-oss® su pokazali biokompatibilni i proliferišući efekat na SHED; dok su dentinski opiljci pokazali blagi citotoksični efekat. Nakon perioda inkubacije od tri nedelje u osteogenom medijumu, SHED su se diferencirale u osteoblaste. To smo dokazali prisustvom obojenih kompleksa u kulturi ćelija nakon bojenja alizarin crvenim i pozitivnim imunofluorecentnim bojanjem osteogenim markerima; ćelije su pokazale visoku ekspresiju anti-Coll1 i Osteopontina, dok je ekspresija Ki-67 i anti-SOX2 antitela bila nešto slabija. Od svih ispitivanih materijala, samo su dentinski opiljci pokazali statistički značajna osteoinduktivna svojstva, eksprimirajući ALPL i RUNX2 gene. Materijal Bio-oss® je u sve tri grupe pokazao ekspresiju oba gena koja je niža od kontrolne grupe, dok je SBA-16/HA samo u grupi ektrakta starog 1. dan imao blago povišenu ekspresiju ALPL gena, gena rane ostoblastične diferencijacije. Zaključak: Iz zubne pulpe mlečnih zuba dece izoluju se ćelije koje imaju visok proliferativni potencijal i potencijal diferencijacije u osteoblaste nakon perioda inkubacije od tri nedelje u osteogenom medijumu. Na osnovu ovog istraživanja, SBA-16/HA i Bio-oss® su pokazali pokazali zadovoljavajuć nivo biokompatibilnosti. Sa malom prednošću SBA-16/HA je bio bolji od primenjivanog zlatnog standarda Bio-oss®. Dentinski opiljci koji se već koriste u kliničkoj praksi su pokazali niži nivo biokompatibilnosti, ali visok osteoinduktivni potencijal. Introduction: Tissue engineering is a multidisciplinary field with a goal to restore, maintain or improve the functions of tissues that are damaged or lost due to various pathological conditions, either by developing adequate biological substitutes or tissue reconstruction. The following factors are very important for success in tissue engineering: choice of stem cell source, strategies used to isolate and passage specific cells, choice of biomaterial to be used as scaffold and the bond between them. Scaffolds can be of different origins, so we have autographs, allografts, xenografts and alloplastic materials. The biomechanical system of bone is complex so that the ideal scaffold should have the following properties: biocompatibility, osteoconductivity, osteoinductivity, ability of angiogenesis. One of the most promising sources of stem cells for the reconstruction of bone damage is dental pulp. Dental pulp stem cells are easily isolated from both deciduous (SHED) and permanent teeth (DPSC), are easily hardened in vitro, and can be used in combination with or without scafold to compensate for bone damage. These cells are extremely proliferative and capable of osteogenic, dentinogenic, adipogenic, chondrogenic, and neurogenic differentiation. The aim of this dissertation was to isolate and characterize mesenchymal stem cells of dental pulp originating from human deciduous teeth that are in their physiological shift, and then determine the biocompatibility of the tested biomaterials (Bio-oss® (Geistlich, Switzerland), dentin particules, and SBA/16-HA), and evaluation of cell viability after contact with them. After that, the goal was to differentiate and immunohistologically confirm the osteblastic transformation of mesenchymal stem cells of dental pulp grown in osteogenic medium, which were later used in the study of the osteonductive potential of different bone substitutes. Material and methods: The experimental part of the research is divided into five parts. In the first part of the research the isolation and characterization of SHED on a flow cytometer was performed, in the second part the synthesis and characterization of SBA-16/HA materials was performed, in the third part of the experiment the biocompatibility of three materials was tested based on standard biocompatibility tests: DET and MTT test. The following materials were tested: Bio-oss® (Geistlich AG, Wolhusen, Switzerland), SBA-16/HA and dentin shavings prepared according to the manufacturer's instructions (BonMaker, KDS, Korea). In the fourth part, differentiation and examination of osteogenic capacity of SHED were performed. Confirmation of the obtained osteoblasts was performed by alizarin red test and immunofluorescent staining of the following primary antibodies: osteopontin, SOX2, Ki-67 and Coll-1. In the last, fifth part of the experimental study, the expression of osteogenic markers in SHED-treated test materials was determined. First, the mRNA was isolated from the cells, then converted to cDNA by reverse transcription, and finally quantified in Real time PCR analysis. The RUNX2 and ALPL genes were used in the analysis. Results: By isolating SHED, we obtained cells that have high proliferative potential and positively express stem cell markers on a flow cytometer, thus meeting the necessary criteria to be defined as human mesenchymal stem cells. In biocompatibility tests, MTT and DET, newly synthesized SBA-16/HA material and control Bio-oss® showed a biocompatible and proliferating effect on SHED; while dentin particules showed a mild cytotoxic effect. After a three-week incubation period in osteogenic medium, SHED differentiated into osteoblasts. We proved this by the presence of stained complexes in cell culture after alizarin staining with red and positive immunofluorescent staining with osteogenic markers; cells showed high expression of anti-Coll 1 and Osteopontin, while expression of Ki-67 and anti-SOX 2 antibodies was slightly lower. Of all the materials tested, only dentin particules showed statistically significant osteoinductive properties, expressing the ALPL and RUNX2 genes. Bio-oss® showed expression of both genes lower than the control group in all three groups, while SBA-16/HA had slightly increased expression of the ALPL gene, an early osteoblastic differentiation gene, only in the 1-day-old extract group. Conclusion: Cells with high proliferative potential and potential for differentiation into osteoblasts after an incubation period of three weeks in osteogenic medium are isolated from the dental pulp of children's deciduous teeth. Based on this study, SBA-16/HA and Bio-oss® showed a satisfactory level of biocompatibility. With a slight advantage, SBA-16/HA was better than the applied Bio-oss® gold standard. Dentin particules already used in clinical practice have shown lower levels of biocompatibility but high osteoinductive potential.

Published
2022

16. Biocompatibility, cytotoxicity and osteoinductivity of different bone substitutes on deciduous tooth pulp stem cells

Author

Bulajić, Dragica, Bajkin, Branislav, Petrović, Bojan, Čapo, Ivan, Savić, Slavica, Selaković, Srećko, and Gušić, Ivana

Subjects
Tissue Engineering, Mesenchymal Stem Cells, Tissue Scaffolds, Biocompatible Materials, Dental Pulp, Tooth, Deciduous, Bone Substitutes, Osteoblasts, Dentin, tkivni inženjering, mezenhimalne stem ćelije, nosači tkiva, biokompatibilni materijali, zubna pulpa, mlečni zubi, zamene za kosti, osteoblasti, dentin
Abstract

Uvod: Tkivni inženjering je multidisciplinarno polje koje ima za cilj da obnovi, održi ili poboljša funkcije tkiva koja su oštećena ili su izgubljena usled različitih patoloških stanja, bilo razvojem adekvatnih bioloških zamena ili rekonstrukcijom tkiva. Za uspeh u inženjeringu tkiva veoma su važni sledeći faktori: izbor izvora matičnih ćelija, strategije koje se koriste za izolovanje i pasažiranje specifičnih ćelija, izbor biomaterijala koji će se koristiti kao skafold (nosač) i povezanost među njima. Skafoldi mogu biti različitog porekla, pa tako imamo autografte, alografte, ksenografte i aloplastične materijale. Biomehanički sistem kosti je složen tako da idealan skafold treba da ispunjava sledeće uslove: biokompatibilnost, osteokonduktivnost, osteoinduktivnost, sposobnost angiogeneze. Jedan od najperspektivnijih izvora matičnih ćelija za rekonstrukciju koštanih oštećenja jeste zubna pulpa. Matične ćelije zubne pulpe se jednostavno izoluju kako iz mlečnih (SHED) tako i stalnih zuba (DPSC), u in vitro uslovima se lako umožavaju i za nadoknadu oštećenja kostiju se mogu koristiti u kombinaciji sa ili bez skafolda. Ove ćelije su izuzetno proliferativne i sposobne za osteogeno, dentinogeno, adipogeno, hondrogeno, i neurogeno diferenciranje. Cilj ove disertacije je bila izolacija i karakterizacija mezenhimalnih matičnih ćelija zubne pulpe poreklom iz humanih mlečnih zuba koji se nalaze u svojoj fiziološkoj smeni, i potom određivanje biokompatibilnosti ispitivanih biomaterijala (Bio-oss® (Geistlich, Switzerland, dentinskih opiljaka i SBA-16/HA ), i evaluacija ćelijske vijabilnosti nakon kontakta sa njima. Nakon toga je cilj bio diferencirati i imunohistološki potvrditi osteoblastičnu transformaciju mezenhimalnih matičnih ćelija zubne pulpe gajenih u osteogenom medijumu, koje su kasnije korišćene u ispitivanju osteoinduktivnog potencijala različitih koštanih zamenika. Materijal i metode: Eksperimentalni deo istraživanja je podeljen na pet delova. U prvom delu istraživanja je vršena izolacija i karakterizacija SHED na protočnom citometru, u drugom delu je vršena sinteza i karakterizacija SBA-16/HA materijala, u trećem delu eksperimenta je vršeno ispitivanje biokompatibilnosti tri materijala na osnovu standardnih testova biokompatibilnosti: DET testa, MTT testa. U četvrtom delu vršena je diferencijacija i ispitivanje osteogenog kapaciteta SHED. Potvrda dobijenih osteoblasta je vršena alizarin red testom i imunofluorescentnim bojenjem sledećih primatnih antitela: osteopontin, SOX2, Ki-67 i Coll-1. U poslednjem, petom delu eksperimentalne studije određivana je ekspresija osteogenih markera u SHED tretiranim ispitivanim materijalima. Najpre je izolovana iRNK iz ćelija, potom je reakcijom reverzne transkripcije prevedena u iDNK i na kraju je kvanitifikovana u Real time PCR analizi. U analizi su korišeni RUNX2 i ALPL geni. Rezultati: Izolovanjem SHED dobili smo ćelije koje imaju visok proliferativni potencijal i pozitivno eksprimiraju markere matičnih ćelija na protočnom citometru, čime ispunjavaju neophodne kriterijume da budu definisane kao humane mezenhimalne stem ćelije. Na testovima biokompatibilnosti, MTT i DET, novosintetisani SBA-16/HA materijal i kontrolni Bio-oss® su pokazali biokompatibilni i proliferišući efekat na SHED; dok su dentinski opiljci pokazali blagi citotoksični efekat. Nakon perioda inkubacije od tri nedelje u osteogenom medijumu, SHED su se diferencirale u osteoblaste. To smo dokazali prisustvom obojenih kompleksa u kulturi ćelija nakon bojenja alizarin crvenim i pozitivnim imunofluorecentnim bojanjem osteogenim markerima; ćelije su pokazale visoku ekspresiju anti-Coll1 i Osteopontina, dok je ekspresija Ki-67 i anti-SOX2 antitela bila nešto slabija. Od svih ispitivanih materijala, samo su dentinski opiljci pokazali statistički značajna osteoinduktivna svojstva, eksprimirajući ALPL i RUNX2 gene. Materijal Bio-oss® je u sve tri grupe pokazao ekspresiju oba gena koja je niža od kontrolne grupe, dok je SBA-16/HA samo u grupi ektrakta starog 1. dan imao blago povišenu ekspresiju ALPL gena, gena rane ostoblastične diferencijacije. Zaključak: Iz zubne pulpe mlečnih zuba dece izoluju se ćelije koje imaju visok proliferativni potencijal i potencijal diferencijacije u osteoblaste nakon perioda inkubacije od tri nedelje u osteogenommedijumu. Na osnovu ovog istraživanja, SBA-16/HA i Bio-oss® su pokazali pokazali zadovoljavajuć nivo biokompatibilnosti. Sa malom prednošću SBA-16/HA je bio bolji od primenjivanog zlatnog standarda Bio-oss®. Dentinski opiljci koji se već koriste u kliničkoj praksi su pokazali niži nivo biokompatibilnosti, ali visok osteoinduktivni potencijal., Introduction: Tissue engineering is a multidisciplinary field with a goal to restore, maintain or improve the functions of tissues that are damaged or lost due to various pathological conditions, either by developing adequate biological substitutes or tissue reconstruction. The following factors are very important for success in tissue engineering: choice of stem cell source, strategies used to isolate and passage specific cells, choice of biomaterial to be used as scaffold and the bond between them. Scaffolds can be of different origins, so we have autographs, allografts, xenografts and alloplastic materials. The biomechanical system of bone is complex so that the ideal scaffold should have the following properties: biocompatibility, osteoconductivity, osteoinductivity, ability of angiogenesis. One of the most promising sources of stem cells for the reconstruction of bone damage is dental pulp. Dental pulp stem cells are easily isolated from both deciduous (SHED) and permanent teeth (DPSC), are easily hardened in vitro, and can be used in combination with or without scafold to compensate for bone damage. These cells are extremely proliferative and capable of osteogenic, dentinogenic, adipogenic, chondrogenic, and neurogenic differentiation. The aim of this dissertation was to isolate and characterize mesenchymal stem cells of dental pulp originating from human deciduous teeth that are in their physiological shift, and then determine the biocompatibility of the tested biomaterials (Bio-oss® (Geistlich, Switzerland), dentin particules, and SBA/16-HA), and evaluation of cell viability after contact with them. After that, the goal was to differentiate and immunohistologically confirm the osteblastic transformation of mesenchymal stem cells of dental pulp grown in osteogenic medium, which were later used in the study of the osteonductive potential of different bone substitutes. Material and methods: The experimental part of the research is divided into five parts. In the first part of the research the isolation and characterization of SHED on a flow cytometer was performed, in the second part the synthesis and characterization of SBA-16/HA materials was performed, in the third part of the experiment the biocompatibility of three materials was tested based on standard biocompatibility tests: DET and MTT test. The following materials were tested: Bio-oss® (Geistlich AG, Wolhusen, Switzerland), SBA-16/HA and dentin shavings prepared according to the manufacturer's instructions (BonMaker, KDS, Korea). In the fourth part, differentiation and examination of osteogenic capacity of SHED were performed. Confirmation of the obtained osteoblasts was performed by alizarin red test and immunofluorescent staining of the following primary antibodies: osteopontin, SOX2, Ki-67 and Coll-1. In the last, fifth part of the experimental study, the expression of osteogenic markers in SHED-treated test materials was determined. First, the mRNA was isolated from the cells, then converted to cDNA by reverse transcription, and finally quantified in Real time PCR analysis. The RUNX2 and ALPL genes were used in the analysis. Results: By isolating SHED, we obtained cells that have high proliferative potential and positively express stem cell markers on a flow cytometer, thus meeting the necessary criteria to be defined as human mesenchymal stem cells. In biocompatibility tests, MTT and DET, newly synthesized SBA-16/HA material and control Bio-oss® showed a biocompatible and proliferating effect on SHED; while dentin particules showed a mild cytotoxic effect. After a three-week incubation period in osteogenic medium, SHED differentiated into osteoblasts. We proved this by the presence of stained complexes in cell culture after alizarin staining with red and positive immunofluorescent staining with osteogenic markers; cells showed high expression of anti-Coll 1 and Osteopontin, while expression of Ki-67 and anti-SOX 2 antibodies was slightly lower. Of all the materials tested, only dentin particules showed statistically significant osteoinductive properties, expressing the ALPL and RUNX2 genes. Bio-oss® showed expression of both genes lower than the control group in all three groups, while SBA-16/HA had slightly increased expression of the ALPL gene, an early osteoblastic differentiation gene, only in the 1-day-old extract group. Conclusion: Cells with high proliferative potential and potential for differentiation into osteoblasts after an incubation period of three weeks in osteogenic medium are isolated from the dental pulp of children's deciduous teeth. Based on this study, SBA-16/HA and Bio-oss® showed a satisfactorylevel of biocompatibility. With a slight advantage, SBA-16/HA was better than the applied Bio-oss® gold standard. Dentin particules already used in clinical practice have shown lower levels of biocompatibility but high osteoinductive potential.

Published
2022

17. The influence of bone mineral density and bisphosphonate therapy on the determinants of oral health and changes on dental panoramic radiographs in postmenopausal women

Author

Grgić, Olja, Bajkin, Branislav, Selaković, Srećko, Đurić, Milanko, Ičin, Tijana, Veljović, Tanja, and Brković, Božidar

Subjects
postmenopauzalna osteoporoza, koštana gustina, oralno zdravlje, parodontopatije, bifosfonati, ortopantomografija, Osteoporosis, Postmenopausal, Bone Density, Oral Health, Periodontal Diseases, Diphosponates, Radiography, Panoramic
Abstract

Prezentovana doktorska disertacija imala je za cilj da utvrdi da li kod pacijentkinja sa osteoporozom postoji veza između mineralne koštane gustine skeleta i pokazatelja oralnog zdravlja, kao i da li su mentalni indeks i mandibularni koštani indeks validni parametri za procenu mineralne koštane gustine skeleta. Materijali i metode: Ispitanice u postmenopauzalnom periodu (N=120) bile su uključene u studiju i podeljene u tri grupe: (I) pacijentkinje obolele od osteoporoze (n=45); (Ib) pacijentkinje obolele od osteoporoze koje uzimaju bisfosfonatnu terapiju (n=45); (K) kontrolna grupa, koju su činile ispitanice sa normalnom koštanom gustinom (n=30). Kod svake ispitanice bili su evaluirani plak indeks, gingivalni i indeks krvarnja, nivo pripojnog epitela (NPE), dubina parodontalnih džepova,kao i KEP indeks (broj karioznih, ekstrahovanih i plombiranih zuba). Mentalni indeks (Mental Index - MI) i Mandibularni kortikalni indeks (Mandibular Cortical Index - MCI) bili su određeni na ortopantomografskim snimcima. Rezultati: Ib grupa je prikazala statistički značajno (p 0,05). Zaključak: Terapija bisfosfonatima bi mogla negativno da utiče na parodontalno zdravlje, ali su potrebna dalja istraživanja kako bi se doneli čvršći zaključci. MI i MCI se nisu pokazali kao dovoljno precizna dijagnostička sredstva u proceni koštane gustine osoba ženskog pola u postmenopauzi., The present PhD thesis aimed to assess the determinants of oral health, in women in the postmenopausal period suffering from osteoporosis (with an emphasis on differences between the patients receiving bisphosphonate therapy), and to examine the correlation between dental panoramic indices (Mental Index - MI, Mandibular Cortical Index - MCI), and bone mineral density in these three groups of patients. Postmenopausal women (N=120) were included in this cross-sectional study and divided in the following groups: (I) women with osteoporosis (n=45), (Ib) women with osteoporosis treated with bisphosphonates (n=45), and (K) control group (n=30). gingival, plaque, and papilla bleeding index, pocket depth, clinical attachment loss (AL), the presence of periodontitis, as well as number of decayed, missing, and filled teeth (DMFT), were evaluated for each patient. The investigated mandibular indices (MI and MCI) were assessed on a dental panoramic radiograph. Group Ib showed significantly higher (p

Published
2021

18. Uticaj preparata koncentrovanih faktora rasta na regeneratorne i reparatorne procese u postekstrakcionim ranama

Author

Tadić, Ana, Mirković, Siniša, Selaković, Srećko, Čolić, Snježana, Puškar, Tatjana, Šarčev, Ivan, and Bajkin, Branislav

Subjects
Third, Wound Healing, Bone Regeneration, treći molar, ekstrakcija zuba, Cone-Beam Computed Tomography, Molar, zarastanje rana, fibrin obogaćen trombocitima, kompjuterska tomografija konusnog zraka, regeneracija kosti, Ne MeSH: koncentrovani faktori rasta, Tooth Extraction, Molar, Third, Platelet-Rich Fibrin, Non MeSH: Concentrated Growth Factors, koncentrovani faktori rasta [Ne MeSH], Concentrated Growth Factors [Non MeSH]
Abstract

Ekstrakcija zuba je intervencija prilikom koje se zub vadi iz svog ležišta u zubnoj alveoli. Rana koja nastaje kao posledica ove intervncije je takvog oblika da njene ivice nije moguće približiti jednu drugoj , te zarasta per secundam intentionem. Ekstrakcija zuba ima za posledicu mnogobrojne promene na tvrdim i mekim tkivima alveolarnog nastavka u periodu od nekoliko meseci do godinu dana nakon intervencije. Savremena stomatologija se i dalje intenzivno bavi proučavanjem procesa zarastanja ekstrakcione rane iz potrebe da se što bolje razumeju promene u tkivu koje nastaju po gubitku zuba da bi se mogle prevenirati i/ili usmeriti tako da se omogući kasnija lakša protetska rehabilitacija pacijenata. Mnoge studije su pokazale efikasnost faktora rasta u tokom procesa zaceljivanja tkiva.Opisan je veliki broj tehnika za pripremu autolognih krvnih preparata koji sadrže faktore rasta, ali su njihova praktična primena i efikasnost su dalje nejasni zato što svaka od ovih metoda dovodi do izrade različitog produkta sa različitom biologijom i potencijalnim indikacijama za upotrebu. Ekstrakcije mandibularnog trećeg molara spadaju u jednu od najčešćih intervencija sa kojom se u svom radu svakodnevno sreću oralni i maksilofacijalni hirurzi. Ova hirurška procedura je povezana za postoperativnim efektima koji u velikoj meri utiču na kvalitet života pacijenta kao što su bol, trizmus, edem, infekcija i alveolitis. U literaturi postoje dokazi da aplikacija nekog od autolognog krvnog preparata sa visokim sadržajem faktora rasta u određenoj meri može da poboljša proces zarastanja tkiva i da umanji neželjene propratne pojave hirurške intervencije nakon ekstrakcije mandibularnog trećeg molara. Cilj ovog rada je bio da utvrdimo da li primena autolognih krvnih preparata sa koncentrovanim faktorima rasta ubrzava stvaranje koštanog tkiva u ekstrakcionoj rani, kao i da li utiče na učestalost pojave alveolitisa i pojavu aproksimalinih parodontalnih džepova na susednim zubima nakon hirurške ekstrakcije donjeg trećeg molara. Studija je sprovedena kao prospektivna klinička studija split-mouth dizajna. U studiju je bilo uključeno 30 pacijenata kod kojih je indikovana ekstrakcija oba mandibularna treća molara i kod kojih su ovi zubi bilateralno u približno istom položaju u odnosu na drugi donji molar. Nakon hirurške ekstrakcije u jednu alveolu je aplikovan preparat koncentrovanih faktora rasta. Kontrolnu grupu u istraživanju činilo je 30 zubnih alveola u koje nije aplikovan preparat koncentrovanih faktora rasta. U eksperimentalnu grupu spadalo je 30 alveola u koje su aplikovani preparati koncentrovanih faktora rasta nakon ekstrakcije zuba. Pacijentima je prvi obavezan kontrolni pregled zakazivan za 7 dana nakon intervencije, tokom koga su uklanjane suture, a vršen je i klinički pregled rane i parodontološkom sondom je proveravana dubina parodontalnog džepa na distalnoj površini drugog molara. Druga postoperativna kontrola je zakazivana 4 nedelje nakon intervencije, a treća nakon 8 nedelja i na ovim kontrolama je merena dubina parodontalnog džepa na distalnoj površini drugog molara. Ispitanicima su napravljena tri CBCT snimka operisanih regija i to po sledećoj dinamici- prvi snimak neposredno nakon ekstrakcije zuba, drugi 4 nedelje i treći 8 nedelja po intervenciji. Svaki CBCT snimak je analiziran da bi se prikupili željeni podaci: zapremina šupljine alveole koja je ostala nakon ekstrakcije zuba i gustina novostvorenog koštanog tkiva, što su parametri na osnovu kojih procenjujemo proces zarastanja koštanog tkiva nakon intervencije. Ova metodologija je originalna , obzirom da su do sada korišćene dvodimenzionalne radiografske metode snimanja sa ciljem praćenja koštanog zarastanja nakon ekstrakcije zuba manje precizne i pouzdane. Dobijenu podaci su obrađeni odgovarajućim matematičko-statističkim postupcima. Najznačajniji rezultati istraživanja su zatim tabelarno i grafički prikazani. Na osnovu dobijenih rezultata došli smo do zaključka da iako primena koncentrovanih faktora rasta dovodi do intenziviranja procesa koštanog zarastanja i smanjenja dubine parodontalnog džepa na distalnoj površini susednih zuba, ta razlika nije statistički značajna. Obzirom da ni u jednom slučaju nije došlo do pojave alveolitisa, nismo mogli zaključiti na koji način primena koncentrovanih faktora rasta utiče na učestalost ove komplikacije., Tooth extraction is an intervention during which a tooth is removed from its socket. A wound that remains after this is of specific size and shape and it heals per secundam intentionem. Where once was a tooth, in following months and years, a large number of changes in composition of hard and soft tissues occure. Haeling of extraction wound in still in focus of contemporary dentistry, since it is imperative to understand all tissue changes in order to prevent and/or gide them and enable prosthodontic rehabilitation of the patient. Many studies confirm a benefitial effect of growth factors douring wound healing. A large number of techniques is developed to prepare autologous blood concentrates containing growth factors, like platelet-rich fibrin (PRF) , but their aplicability and efficancy are still unclear because each of these methods results in product with different biology and physical characteristics, as well as different potential indications. Third mandibular molar extraction is one of the most frequent interventions that oral and maxillofacial surgeon face in their everyday clinical practice. This procedure is usually followed by postoperative effects affecting such as pain, trismus, edema, infection and alveolitis. In contemporary literature there is enough evidence to suport beneficial role of autologous blood preparations in wound healing, and some authors even sugest that they can reduce incidence of postextraction complications afther third molar surgery. The aim of this study was to determin weather concentrated growth factors have beneficial effect on bone healing after tooth extraction, as well as their effect on the incidence of alveolitis and do they reduce pocket depth on distal side of adjacent tooth. This study was conducted as prospective clinical split-mouth designed study. 30 patients with both mandibular third molars indicated for the extraction, in similar position, were included in the study. On the same day surgical removal of both mandibular molars was performed, and in one socket PRF was placed. Patients were scheduled for a check-up and suture removals on the 7th postoperative day. During this visit, as well as after 4 and after 8 weeks, depth of distal pocket of the second molar was measured. CBCT was made on the day of surgery, 4 and 8 weeks afther surgery. On these radiographs volume of the bone defect was measured as well as density of newly formed bone tissue in the socket. This is original methodology, while previous studies used two-dimensional radiography methods in order to evaluate bone healing after tooth extractions, with less precision and liability. We processed and analyzed gained data using appropriate mathematical-statistical methods. According to our data we concluded that application of PRF in the extraction socket improves bone healing and reduces depth of pocket on the adjacent teeth, although this effects are not statistically significant. In our study, alveolitis did not occur neither in control nor in the experimental group, so we could not conclude if the application of PRF has any effect on prevention of this complication.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results