Search

Your search keyword '"Selber, F."' showing total 3 results
Start Over Author "Selber, F."
3 results on '"Selber, F."'

1. Presence of the Gpr179(nob5) allele in a C3H-derived transgenic mouse

Author

Balmer J, Ji R, Ta, Ray, Selber F, Gassmann M, Ns, Peachey, Ronald Gregg, Enzmann V, University of Zurich, and Enzmann, Volker

Subjects
Male, Mice, Inbred C3H, Retinal Bipolar Cells, Gene Expression, Mice, Transgenic, 10081 Institute of Veterinary Physiology, 2731 Ophthalmology, Founder Effect, Receptors, G-Protein-Coupled, Disease Models, Animal, Mice, Mutagenesis, Insertional, Night Blindness, Electroretinography, Animals, Humans, 570 Life sciences, biology, Female, Transgenes, Erythropoietin, Alleles, Crosses, Genetic, Research Article, Signal Transduction
Abstract

PURPOSE: To identify the mutation responsible for an abnormal electroretinogram (ERG) in a transgenic mouse line (tg21) overexpressing erythropoietin (Epo). The tg21 line was generated on a mixed (C3H; C57BL/6) background and lacked the b-wave component of the ERG. This no-b-wave (nob) ERG is seen in other mouse models with depolarizing bipolar cell (DBC) dysfunction and in patients with the complete form of congenital stationary night blindness (cCSNB). We determined the basis for the nob ERG phenotype and screened C3H mice for the mutation to evaluate whether this finding is important for the vision research community. METHODS: ERGs were used to examine retinal function. The retinal structure of the transgenic mice was investigated using histology and immunohistochemistry. Inverse PCR was performed to identify the insertion site of the Epo transgene in the mouse genome. Affected mice were backcrossed to follow the inheritance pattern of the nob ERG phenotype. Quantitative real-time PCR (qRT PCR), Sanger sequencing, and immunohistochemistry were used to identify the mutation causing the defect. Additional C3H sublines were screened for the detected mutation. RESULTS: Retinal histology and blood vessel structure were not disturbed, and no loss of DBCs was observed in the tg21 nob mice. The mutation causing the nob ERG phenotype is inherited independently of the tg21 transgene. The qRT PCR experiments revealed that the nob ERG phenotype reflected a mutation in Gpr179, a gene involved in DBC signal transduction. PCR analysis confirmed the presence of the Gpr179(nob5) insertional mutation in intron 1 of Gpr179. Screening for mutations in other C3H-derived lines revealed that C3H.Pde6b(+) mice carry the Gpr179 (nob5) allele whereas C3H/HeH mice do not. CONCLUSIONS: We identified the presence of the Gpr179(nob5) mutation causing DBC dysfunction in a C3H-derived transgenic mouse line. The nob phenotype is not related to the presence of the transgene. The Gpr179(nob5) allele can be added to the list of background alleles that impact retinal function in commonly used mouse lines. By providing primers to distinguish between Gpr179 mutant and wild-type alleles, this study allows investigators to monitor for the presence of the Gpr179(nob5) mutation in other mouse lines derived from C3H.

Published
2016

3. Presence of the Gpr179(nob5) allele in a C3H-derived transgenic mouse.

Author

Balmer J, Ji R, Ray TA, Selber F, Gassmann M, Peachey NS, Gregg RG, and Enzmann V

Subjects
Animals, Crosses, Genetic, Disease Models, Animal, Electroretinography, Erythropoietin metabolism, Female, Founder Effect, Gene Expression, Humans, Male, Mice, Mice, Inbred C3H, Mice, Transgenic, Night Blindness metabolism, Night Blindness pathology, Receptors, G-Protein-Coupled metabolism, Retinal Bipolar Cells pathology, Signal Transduction, Transgenes, Alleles, Erythropoietin genetics, Mutagenesis, Insertional, Night Blindness genetics, Receptors, G-Protein-Coupled genetics, Retinal Bipolar Cells metabolism
Abstract

Purpose: To identify the mutation responsible for an abnormal electroretinogram (ERG) in a transgenic mouse line (tg21) overexpressing erythropoietin (Epo). The tg21 line was generated on a mixed (C3H; C57BL/6) background and lacked the b-wave component of the ERG. This no-b-wave (nob) ERG is seen in other mouse models with depolarizing bipolar cell (DBC) dysfunction and in patients with the complete form of congenital stationary night blindness (cCSNB). We determined the basis for the nob ERG phenotype and screened C3H mice for the mutation to evaluate whether this finding is important for the vision research community., Methods: ERGs were used to examine retinal function. The retinal structure of the transgenic mice was investigated using histology and immunohistochemistry. Inverse PCR was performed to identify the insertion site of the Epo transgene in the mouse genome. Affected mice were backcrossed to follow the inheritance pattern of the nob ERG phenotype. Quantitative real-time PCR (qRT PCR), Sanger sequencing, and immunohistochemistry were used to identify the mutation causing the defect. Additional C3H sublines were screened for the detected mutation., Results: Retinal histology and blood vessel structure were not disturbed, and no loss of DBCs was observed in the tg21 nob mice. The mutation causing the nob ERG phenotype is inherited independently of the tg21 transgene. The qRT PCR experiments revealed that the nob ERG phenotype reflected a mutation in Gpr179, a gene involved in DBC signal transduction. PCR analysis confirmed the presence of the Gpr179(nob5) insertional mutation in intron 1 of Gpr179. Screening for mutations in other C3H-derived lines revealed that C3H.Pde6b(+) mice carry the Gpr179 (nob5) allele whereas C3H/HeH mice do not., Conclusions: We identified the presence of the Gpr179(nob5) mutation causing DBC dysfunction in a C3H-derived transgenic mouse line. The nob phenotype is not related to the presence of the transgene. The Gpr179(nob5) allele can be added to the list of background alleles that impact retinal function in commonly used mouse lines. By providing primers to distinguish between Gpr179 mutant and wild-type alleles, this study allows investigators to monitor for the presence of the Gpr179(nob5) mutation in other mouse lines derived from C3H.

Published
2013

Catalog

Books, media, physical & digital resources
See catalog results