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3 pages.-- 2nd Caparica Christmas Conference on Sample Treatment, Caparica – Portugal, 5th – 7th December 2016, A new strategy for the fast monitoring of peptide biomarkers is described. It is based on the use of accelerated in-solution trypsin digestions under an ultrasonic field provided by high-intensity focused ultrasound (HIFU) and the monitoring of several peptides by selected MS/MS ion monitoring in a linear ion trap mass spectrometer. The performance of the method was established for the unequivocal identification of all commercial fish species belonging to the Merlucciidae family. Using a particular combination of only 11 peptides, resulting from the HIFU-assisted tryptic digestion of the thermostable proteins parvalbumins, the workflow allowed the unequivocal identification of these closely related fish species in any seafood product, including processed and precooked products, in less than 2 h. The present strategy constitutes the fastest method for peptide biomarker monitoring. Its application for food quality control provides to the authorities an effective and rapid method of food authentication and traceability to guarantee the quality and safety to the consumers

10 páginas, 5 figuras, 2 tablas, Parvalbumins beta (β-PRVBs) are considered the majorfishallergens. A new strategy for the rapid and directdetection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the directdetection of the presence of fish β-PRVBs in any food product in less than 2 hours. This new affordable assay would be very useful for the sanitary and inspect authorities to protect sensitive consumers from allergic reactions and to guarantee their safety, This work was supported by the Comisión Interministerial de Ciencia y Tecnología (CICyT) (Project AGL2000-0440-P4-02). Dr Mónica Carrera is supported by the Barrié Fundation