1. Rapid Analysis of the Chemical Compositions in Semiliquidambar cathayensis Roots by Ultra High-Performance Liquid Chromatography and Quadrupole Time-of-Flight Tandem Mass Spectrometry
- Author
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Junwei He, Li Yang, and Ronghua Liu
- Subjects
chemical compositions ,Flavonoid ,Phytochemicals ,Pharmaceutical Science ,Tandem mass spectrometry ,01 natural sciences ,Semiliquidambar cathayensis roots ,UHPLC-Q-TOF-MS/MS ,Plant Roots ,Article ,Analytical Chemistry ,Drug Discovery ,Physical and Theoretical Chemistry ,Quadrupole time of flight ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Semiliquidambar cathayensis ,Chromatography ,Phenylpropanoid ,biology ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Plant Extracts ,010401 analytical chemistry ,Organic Chemistry ,biology.organism_classification ,Terpenoid ,Cyclic peptide ,chemical profiling ,0104 chemical sciences ,Hamamelidaceae ,Chemistry (miscellaneous) ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Molecular Medicine ,Ultra high performance ,rapid identification - Abstract
Semiliquidambar cathayensis Chang was a traditional medicinal plant and used to treat rheumatism arthritis and rheumatic arthritis for centuries in China with no scientific validation, while only 15 components were reported. Thus, a rapid, efficient, and precise method based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied in both positive- and negative-ion modes to rapidly analysis the main chemical compositions in S. cathayensis for the first time. Finally, a total of 85 chemical compositions, including 35 alkaloids, 12 flavonoids, 7 terpenoids, 5 phenylpropanoids, 9 fatty acids, 7 cyclic peptides, and 10 others were identified or tentatively characterized in the roots of S. cathayensis based on the accurate mass within 5 ppm error. Moreover, alkaloid, flavonoid, phenylpropanoid, and cyclic peptide were reported from S. cathayensis for the first time. This rapid and sensitive method was highly useful to comprehend the chemical compositions and will provide scientific basis for further study on the material basis, mechanism and clinical application of S. cathayensis roots.
- Published
- 2019