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To understand the pollution characteristics, spatial distribution characteristics, potential ecological risks, and sources of heavy metals in surface sediments of Dongjiang Lake, 12 surface sediment samples were collected from Dongjiang Lake. The contents of 20 heavy metals including Li, Be, V, Cr, Mn, Co, Ni, Cu, Zn, Ga, As, Se, Rb, Sr, Ag, Cd, Cs, Ba, Pb, and U were analyzed in this study. The geo-accumulation index method and potential risk index method were used to evaluate the pollution degree and potential risks of heavy metals in sediments, and the correlation analysis and principal component analysis were used to trace the source of the primary heavy metals. The results showed that the average contents of

This study intended to investigate the role of microRNA-27b (miR-27b) in proliferation of oral squamous cell carcinoma (OSCC) cells and to explore the potential molecular mechanism. Cell proliferation was detected by MTT assay. The expression levels of miR-27b, Frizzled7 (FZD7), cyclin D1 and c-myc were detected by quantitative real time polymerase chain reaction (qRT-PCR). The protein expression level of FZD7 was detected by western blot analysis. The relationship between miR-27b and FZD7, and the activity of Wnt signaling pathway were determined using luciferase reporter assay. The miR-27b expression in OSCC cell lines was significantly decreased compared with control. Overexpression of miR-27b remarkably inhibited OSCC cell proliferation. Additionally, miR-27b could target and inhibit FZD7 expression and decrease the activity of Wnt signaling pathway.miR-27b could inhibit OSCC cell proliferation through inhibiting FZD7 and FZD7-mediated Wnt signaling pathway.

To investigate the effect of growth factor receptor-bound 7 (Grb7) on oral squamous cell carcinoma growth and tumor xenografts.Cal27 and hNOK cells were cultivated, real-time PCR and Western blotting were used to detect the expression of Grb7 in hNOK and Cal27. Cal27 was transfected with Grb7 siRNA for 48 h, cell proliferation was assayed using MTT. Flow cytometry was used to determine the cell cycle and apoptosis. Western blot was used to evaluate the expression of caspase3, Bax, bcl-2, Cyclin D1, Rb, E2F1, ERK and FOXM1. Grb7 siRNA and negative control were designed and injected subcutaneously into the mice, tumor volume and weight were measured. Statistical analysis was performed using SPSS 11.0 software package.Grb7 was highly expressed in Cal27 compared with hNOK. Depletion of Grb7 significantly inhibited cell proliferation, blocked G1/S phase transition, promoted cell apoptosis. Knockdown of Grb7 suppressed the expression of Cyclin D1 and Rb, upregulated E2F1 expression. Moreover, c-caspase 3 and Bax was also reduced after inhibition of Grb7. ERK/FOXM1 signaling pathway was also inhibited by Grb7. In addition, the volume and weight of tumor xenografts were reduced by siGrb7.Depletion of Grb7 inhibits cell proliferation, promotes apoptosis and reduces tumor xenografts through ERK/FOXM1 pathway.

In the context of south china electricity market, the concept of contract transfer and replacement deal is set forth in advance which is specialized in electricity treatment. Then, the feasibility of conducting an inter-provincial contract transfer and replacement is analyzed according to the aspects of physical characteristics, economics and policies within the china southern power grid. In addition, the advantage and disadvantage are also presented in terms of bilateral negotiation and centralized biding transaction modes. After that, the pricing mechanism and transaction mode are deliberately designed pertaining to three different situations including a party power shortage and a party "abandon water", a party power supply shortage and a party power surplus and a party power surplus and a party "abandon water". Finally, the realistic feasibility of the proposed schemes is verified through an employed numerical example.

In order to solve the need of the oil and gas pipeline defect quantification in the real-time online defecting, magnetic flux leakage inspection method was applied to oil and gas pipeline inspection. According to the basic theory of the electromagnetic field, finite element solution of electromagnetic field and ANSYS electromagnetic field calculation theory, using the function of ANSYS 's simulation and calculation for magnetic field, three-dimensional finite element model of the oil and gas pipeline defect was built up. Through simulating, the relationship between defect signal and defect size was found, the optimal distance of the hall sensor lift-off value was verified, the best magnetization of leakage magnetic field was discussed, and various factors to influence the magnetic flux leakage signal is analyzed.

A design method and primary functions of pattern preparation system of computerized flat knitting machine are introduced. The system provides a formidable mapping system based on object-oriented and multi-document, so pattern becomes simple and flexible to be edited and displayed. In addition, the compiler has a novel method of compiling pattern data. Two pattern description files (PAT and CNT) are generated after compiled, which include all control information that computerized flat knitting machine needs. The system also provides a direct and friendly interface, through which user can accomplish pattern design quickly and conveniently.

The use of custom implants has recently gained increasing importance in craniofacial surgery in order to optimize preoperative planning and reduce operative time. The design of patient-specific implants plays an important role to restore craniofacial bone. Nevertheless, the design method has become the bottleneck. In this study, a new design method of a maxillofacial prosthesis based on the topology optimization was proposed to realize lightweight custom implants. At first, parametric predesign of the optimized model was carried out to ensure that the optimized model conformed to the main physiological anatomical structure and recover basic function structure. Then, the design region is defined under given load and boundary conditions. The material interpolation technique of SIMP with the penalization factor is adopted to get the final optimized 3D model. Finally, the experimental results verify that the method of topology optimization for the maxillofacial prosthesis proposed is efficient.

Adenoid cystic carcinoma (ACC) is a common type of salivary gland cancer, characterized by slow growth but high incidence of distant metastasis. Distant metastasis occurs in 25%–50% of ACC patients and more commonly in lungs. However, the molecular mechanism underlying the aggressive growth of ACC remains obscure. Human tumor cell lines are extremely useful in studying cancer biology, under both in vitro and in vivo condition. Unfortunately, the tumor cell lines are susceptible to cross-contamination, by other cell lines, during routine culture. Recent studies have proved that most of the ACC cell lines, established for preclinical research, are either cross-contaminated or nonhuman cells. Here, we review the literature assessing the identity of ACC cell lines, dated up to September 1, 2016, using PubMed, EMBASE, and the China National Knowledge Infrastructure databases with the following keywords: “adenoid cystic carcinoma,” “cell line,” and “characterization” or “contamination.” According to the results, only salivary adenoid cystic carcinoma (SACC)-83/SACC-lung metastasis, the HPV-transformed ACC-112 and the two newly established moderately differentiated adenocarcinoma-ACC-01 and LACC-1 cell lines are authentic ACC cell lines. These results suggest that, in the future, it is crucial to authenticate the ACC cell lines before research.

To investigate the method and efficacy of reconstruction of anterior mouth floor defects with depressor labii inferioris flap.Between March 2010 and August 2011, 4 patients with cancer in the anterior part of oral floor underwent tumour resection and simultaneous reconstruction with depressor labii inferioris flap. The patients were followed up for 2 - 18 months. The survival of the flaps, mobility of tongue and esthetic outcome of lip and mental region were evaluated.All the flaps survived completely, tongues moved well, and functional and esthetic results were good.The depressor labii inferioris flaps are suitable for repairing defects of anterior oral floor.

To study the anatomy of supraclavicular artery island flap and report the clinical application of the island flap for the reconstruction of tongue defects.The branch, origination, course, length and diameter of transverse cervical artery and supraclavicular artery were observed on 20 flaps of 10 adult cadavers perfused with lead oxide-gel, and the draining veins were also observed. The supraclavicular artery island flaps were used to reconstruct the defects following tongue cancer ablation in 4 patients, and the data concerning functional impairment, aesthetic outcome and donor site morbidity were analyzed.The transverse cervical artery were originated from the thyrocervical trunk or subclavical artery, and separated into deep branch and superficial branch above the middle third of the clavicle. The supraclavicular artery were originated from superficial branch of the transverse cervical artery and extended to backward and outward, and run over surface of trapezius, acromial end of clavicle and deltoid fascia, and then penetrated the deep fascia and go into skin and subcutaneous tissue of supraclavicular and shoulder regions. The distance between the origins of the supraclavicular and transverse cervical arteries was on average 4.3 cm, and the distance between the origin of supraclavicular artery and the point where it penetrated the deep fascia was on average 3.6 cm. The external diameter of the transverse cervical artery was on average 2.7 mm, and the external diameter of supraclavicular artery was on average 1.1 mm. Two supraclavicular comitant veins ran adjacent to the supraclavicular artery and drained into the transverse cervical vein and external jugular vein respectively. In the clinical study, 3 flaps survived completely and part of the skin paddle of the flap in one case exhibited necrosis. The contour and function of tongues were restored well and there was no limitation of shoulder motion in all 4 cases.The supraclavicular artery island flap with supraclavicular artery as nutrient vessel is reliable for reconstruction of oral, maxillofacial and neck defects, and it can be used as a free flap equipped with the transverse cervical artery.

Flight restraint is important for zoos, safaris, and breeding centers for large birds. Currently used techniques for flight restraint include both surgical and non-surgical approaches. Surgical approaches usually cause permanent change to or removal of tendon, patagial membrane, or wing bones, and can cause pain and inflammation. Non-surgical approaches such as clipping or trimming feathers often alter the bird's appearance, and can damage growing blood feathers in fledglings or cause joint stiffness. We observed microstructure of primary feathers of the red-crowned crane (Grus japonensis) and found that the width of barbs is a determinative factor influencing vane stiffness and geometric parameters. We hypothesized that partial longitudinal excision of barbs on the ventral surface of the primary feathers would reduce the stiffness of the vane and render the feathers unable to support the crane's body weight during flight. Furthermore, we hypothesized that this modification of barbs would also change the aerodynamic performance of feathers such that they could not generate sufficient lift and thrust during flapping to enable the bird to fly. We tested this hypothesis on a red-crowned crane that had normal flight capability by excising the ventral margin of barbs on all 10 primaries on the left wing. The bird was unable to take off until the modified feathers were replaced by new ones. Removal of barbs proved to be a simple, non-invasive, low-cost and reversible method for flight restraint. It is potentially applicable to other large birds with similar structural characteristics of primary feathers.

To evaluate the effect of intralesional injection of Pingyangmycin (PYM) for treatment of venous-venular malformation in the maxillofacial regions of children.The medical records of 306 patients with venous-venular malformation in the maxillofacial regions between January 1997 and June 2005 were reviewed. One hundred and twenty-three of the patients were men, and one hundred and eighty-three were woman, the sex ratio was 1:1.49. The patients aged from one month to 12 years, 172 were less than 6 months (56.21%). Pingyangmycin was injected into the center or in the vicinity of the venous-venular malformations, and repeated every 7 to 10 days. This cycle were repeated 3 to 5 times when necessary.Three hundred and six patients were followed up for 6 to 102 months. Complete response was obtained in 156 cases (50.98%), partial response in 116 cases (37.91%), and improvement in 34 cases (11.11%). The overall response rate was 100%, and the curative rate was 88.89%.This method is safe, simple and effective for venous-venular malformation in the maxillofacial regions of children.

The effect of Pinyangmycin, Dexamethasonum and Sodium Morrhuate injected concomitantly to treat cavernous hemangioma in oral and maxillofacial regions was evaluated.The medical records of 350 patients with cavernous hemangioma in oral and maxillofacial regions between January 1998 and January 2002 were reviewed. One hundred and sixty-five of the patients were men, and 185 were women, the sex ratio was 1:1.12. The patients' ages were between 4 months and 55 years. Two hundred and Twenty-six hemangiomas were located in the maxillofacial regions, and 124 located in the oral cavity. The size of the lesions varied from 1 cm x 1 cm to 6 cm x 9 cm. Pinyangmycin, Dexamethasonum and Sodium Morrhuate were injected simultaneously into the lesions or in the vicinity of the cavernous hemangiomas, and the injection might be repeated every 5 to 7 days when necessary, and this process could be repeated 3 to 5 times.Three hundred and fifty patients were followed up for 6-48 months. The cure and essentially cured rates were 95.48%, the total efficiency rate was 100%.This method was a safe, simple and effective therapy to manage cavernous hemangioma in the oral and maxillofacial regions.