1. Serum lncRNA ITGB2-AS1 and ICAM-1 as novel biomarkers for rheumatoid arthritis and osteoarthritis diagnosis.
- Author
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Selim AM, Elsabagh YA, El-Sawalhi MM, Ismail NA, and Senousy MA
- Subjects
- Humans, Male, Female, Middle Aged, Case-Control Studies, Osteoarthritis blood, Osteoarthritis genetics, Osteoarthritis diagnosis, Aged, Osteoarthritis, Knee blood, Osteoarthritis, Knee genetics, Osteoarthritis, Knee diagnosis, Adult, Arthritis, Rheumatoid blood, Arthritis, Rheumatoid genetics, Arthritis, Rheumatoid diagnosis, Intercellular Adhesion Molecule-1 blood, Intercellular Adhesion Molecule-1 genetics, Biomarkers blood, RNA, Long Noncoding blood, RNA, Long Noncoding genetics
- Abstract
Background: The complete circulating long non-coding RNAs (lncRNAs) signature of rheumatoid arthritis (RA) and osteoarthritis (OA) is still uncovered. The lncRNA integrin subunit beta 2 (ITGB2)-anti-sense RNA 1 (ITGB2-AS1) affects ITGB2 expression; however, there is a gap in knowledge regarding its expression and clinical usefulness in RA and OA. This study investigated the potential of serum ITGB2-AS1 as a novel diagnostic biomarker and its correlation with ITGB2 expression and its ligand intercellular adhesion molecule-1 (ICAM-1), disease activity, and severity in RA and primary knee OA patients., Subjects: Forty-three RA patients, 35 knee OA patients, and 22 healthy volunteers were included., Results: Compared with healthy controls, serum ITGB2-AS1 expression was upregulated in RA patients but wasn't significantly altered in knee OA patients, whereas serum ICAM-1 protein levels were elevated in both diseases. ITGB2-AS1 showed discriminative potential for RA versus controls (AUC = 0.772), while ICAM-1 displayed diagnostic potential for both RA and knee OA versus controls (AUC = 0.804, 0.914, respectively) in receiver-operating characteristic analysis. In the multivariate analysis, serum ITGB2-AS1 and ICAM-1 were associated with the risk of developing RA, while only ICAM-1 was associated with the risk of developing knee OA. A panel combining ITGB2-AS1 and ICAM-1 showed profound diagnostic power for RA (AUC = 0.9, sensitivity = 86.05%, and specificity = 91.67%). Interestingly, serum ITGB2-AS1 positively correlated with disease activity (DAS28) in RA patients and with ITGB2 mRNA expression in both diseases, while ICAM-1 positively correlated with ITGB2 expression in knee OA patients., Conclusion: Our study portrays serum ITGB2-AS1 as a novel potential diagnostic biomarker of RA that correlates with disease activity. A predictive panel combining ITGB2-AS1 and ICAM-1 could have clinical utility in RA diagnosis. We also spotlight the association of ICAM-1 with knee OA diagnosis. The correlation of serum ITGB2-AS1 with ITGB2 expression in both diseases may be insightful for further mechanistic studies., (© 2024. The Author(s).)
- Published
- 2024
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