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11 results on '"Seo, Sun Il"'

3. Biocontrol of the causal brown patch pathogen Rhizoctonia solani by Bacillus velezensis GH1-13 and development of a bacterial strain specific detection method

Author

Lee, Gahee, primary, Choi, Hyeongju, additional, Liu, Haifeng, additional, Han, Yun-Hyeong, additional, Paul, Narayan Chandra, additional, Han, Gui Hwan, additional, Kim, Hyunsook, additional, Kim, Pyoung Il, additional, Seo, Sun-Il, additional, Song, Jaekyeong, additional, and Sang, Hyunkyu, additional

Published
2023
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11. Enhanced indirubin production in recombinant Escherichia coli harboring a flavin-containing monooxygenase gene by cysteine supplementation

Author

Han, Gui Hwan, Gim, Geun Ho, Kim, Wonduck, Seo, Sun Il, and Kim, Si Wouk

Subjects
*INDIRUBIN, *RECOMBINANT proteins, *ESCHERICHIA coli, *FLAVINS, *MONOOXYGENASES, *CYSTEINE, *YEAST extract, *TRYPTOPHAN
Abstract

Abstract: In our previous study, a batch fermentation of recombinant Escherichia coli DH5α cells harboring the fmo gene from Methylophaga aminisulfidivorans MPT produced indirubin (5.0mg/L) and indigo (920mg/L) in a 5L fermenter containing tryptophan medium (2g/L tryptophan, 5g/L yeast extract, 10g/L NaCl). In this study, it was found that indirubin production greatly increased when 0.36g/L cysteine was added to the tryptophan medium, although cysteine inhibited the growth of the recombinant E. coli harboring the fmo gene. However, the addition of cysteine did not inhibit the expression level and activity of FMO in the cell. Indigo was synthesized by the dimerization of two 3-hydroxyindole molecules under the non-enzymatic reaction. Cysteine influenced the regioselectivity of FMO and enhanced the synthesis of 2-hydroxyindole instead of 3-hydroxyindole, which might function to increase indirubin production. The optimal culture conditions for indirubin production in tryptophan medium were determined from the response surface methodology analysis: 2g/L tryptophan, 5g/L yeast extract, 10g/L NaCl, 0.36g/L (3mM) cysteine, pH 8.0 at 35°C. Under these conditions, the recombinant E. coli cells were capable of producing 223.6mg/L of indirubin from 2g/L of tryptophan. The intracellular accumulation of the indirubin crystals might stress the cell, which may be a main reason for the poor growth of the recombinant E. coli pBlue 1.7. [Copyright &y& Elsevier]

Published
2012
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