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6. Distribution and properties of fructosyl amino acid oxidase in fungi

Author

Yoshida, Nobuyuki, Sakai, Yasuyoshi, Serata, Masaki, Tani, Yoshiki, and Kato, Nobuo

Subjects
Fungi -- Research, Oxidases -- Observations, Biological sciences
Abstract

The Aspergillus, Fusarium, Gibberella, and Penicillium genera contain fructosyl amino acid oxidase. The substrate specificity of the enzyme against fructosyl valine and N(super epsilon)-fructosyl N(super alpha)-Z-lysine is different in different fungi. Penicillium janthinellum AKU3413 contains a fructosyl valine-specific monomeric enzyme. The enzyme is most active at 25 degree celsius and 7.5 pH. It contains a flavin adenine dinucleotide bound covalently as a prosthetic group. The purified enzyme shows two absorption peak at 366 and 448 nm which is typical of flavin compounds.

Published
1995

7. Suppressive effect on activation of macrophages by Lactobacillus casei strain shirota genes determining the synthesis of cell wall-associated polysaccharides

Author

Yasuda, Emi, Serata, Masaki, and Sako, Tomoyuki

Subjects
Polysaccharides -- Genetic aspects, Chemical synthesis -- Analysis, Lactobacillus -- Genetic aspects, Biological sciences
Abstract

A study was conducted to explore the function of polysaccharide moiety of the cell wall of Lactobacillus casei strain Shirota as a possible immune modulator that regulates cytokine production by macrophages. The studies identified a cluster of genes from L. casei Shirota involved in the biosynthesis of the high-molecular-mass polysaccharide, as the relevant immune modulator might function to reduce excessive immune reactions during the activation of macrophages by L. casei Shirota.

Published
2008

11. Probiotic upregulation of peripheral IL-17 responses does not exacerbate neurological symptoms in experimental autoimmune encephalomyelitis mouse models

Author

Kobayashi, Toshihide, primary, Suzuki, Tomomi, additional, Kaji, Rumi, additional, Serata, Masaki, additional, Nagata, Tsuyoshi, additional, Ando, Minoru, additional, Iizuka, Ryoko, additional, Tsujibe, Satoshi, additional, Murakami, Junta, additional, Kiyoshima-Shibata, Junko, additional, Kato, Ikuo, additional, Nanno, Masanobu, additional, and Shida, Kan, additional

Published
2011
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13. Coulomb Excitation of24Si

Author

Kanno, Shouko, primary, Gomi, Tomoko, additional, Motobayashi, Tohru, additional, Yoneda, Ken-ichiro, additional, Aoi, Nori, additional, Ando, Yoshiaki, additional, Baba, Hidetada, additional, Demichi, Kimihiko, additional, Fülöp, Zsolt, additional, Futakami, Udai, additional, Hasegawa, Hirokazu, additional, Higurashi, Yoshihide, additional, Ieki, Kazuo, additional, Imai, Nobuaki, additional, Iwasa, Naohito, additional, Iwasaki, Hironori, additional, Kubo, Toshiyuki, additional, Kubono, Shigeru, additional, Kunibu, Makoto, additional, Matsuyama, Yuuichi U., additional, Michimasa, Shin'ichiro, additional, Minemura, Toshiyuki, additional, Murakami, Hiroyuki, additional, Nakamura, Takashi, additional, Saito, Akito, additional, Sakurai, Hiroyoshi, additional, Serata, Masaki, additional, Shimoura, Susumu, additional, Sugimoto, Takashi, additional, Takeshita, Eri, additional, Takeuchi, Satoshi, additional, Ue, Koji, additional, Yamada, Kazunari, additional, Yanagisawa, Yoshiyuki, additional, Yoshida, Atsushi, additional, and Ishihara, Masayasu, additional

Published
2002
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14. Molecular States in Neutron-Rich Beryllium Isotopes

Author

Saito, Akito, primary, Shimoura, Susumu, additional, Takeuchi, Satoshi, additional, Motobayashi, Tohru, additional, Akiyoshi, Hiromichi, additional, Ando, Yoshiaki, additional, Aoi, Nori, additional, Fülöp, Zsolt, additional, Gomi, Tomoko, additional, Higurashi, Yoshihide, additional, Hirai, Masaaki, additional, Iwasa, Naohito, additional, Iwasaki, Hironori, additional, Iwata, Yoshiyuki, additional, Kobayashi, Hiroshi, additional, Kurokawa, Meiko, additional, Liu, Zhong, additional, Minemura, Toshiyuki, additional, Ozawa, Shuichi, additional, Sakurai, Hiroyoshi, additional, Serata, Masaki, additional, Teranishi, Takashi, additional, Yamada, Kazunari, additional, Yanagisawa, Yoshiyuki, additional, and Ishihara, Masayasu, additional

Published
2002
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15. Inelastic Scattering of12Be with4He

Author

Matsuyama, Yuuichi U., primary, Motobayashi, Tohru, additional, Shimoura, Susumu, additional, Minemura, Toshiyuki, additional, Saito, Akito, additional, Baba, Hidetada, additional, Akiyoshi, Hiromichi, additional, Aoi, Nori, additional, Ando, Yoshiaki, additional, Gomi, Tomoko, additional, Higurashi, Yoshihide, additional, Ieki, Kazuo, additional, Imai, Nobuaki, additional, Iwasa, Naohito, additional, Iwasaki, Hironori, additional, Kanno, Shouko, additional, Kubono, Shigeru, additional, Kunibu, Makoto, additional, Michimasa, Shin'ichiro, additional, Murakami, Hiroyuki, additional, Nakamura, Takashi, additional, Sakurai, Hiroyoshi, additional, Serata, Masaki, additional, Takeshita, Eri, additional, Takeuchi, Satoshi, additional, Teranishi, Takashi, additional, Ue, Koji, additional, Yamada, Kazunari, additional, and Yanagisawa, Yoshiyuki, additional

Published
2002
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16. Coulomb Dissociation of23Al

Author

Gomi, Tomoko, primary, Motobayashi, Tohru, additional, Yoneda, Ken-ichiro, additional, Kanno, Shouko, additional, Aoi, Nori, additional, Ando, Yoshiaki, additional, Baba, Hidetada, additional, Demichi, Kimihiko, additional, Fülöp, Zsolt, additional, Futakami, Udai, additional, Hasegawa, Hirokazu, additional, Higurashi, Yoshihide, additional, Ieki, Kazuo, additional, Imai, Nobuaki, additional, Iwasa, Naohito, additional, Iwasaki, Hironori, additional, Kubo, Toshiyuki, additional, Kubono, Shigeru, additional, Kunibu, Makoto, additional, Matsuyama, Yuuichi U., additional, Michimasa, Shin'ichiro, additional, Minemura, Toshiyuki, additional, Murakami, Hiroyuki, additional, Nakamura, Takashi, additional, Saito, Akito, additional, Sakurai, Hiroyoshi, additional, Serata, Masaki, additional, Shimoura, Susumu, additional, Sugimoto, Takashi, additional, Takeshita, Eri, additional, Takeuchi, Satoshi, additional, Ue, Koji, additional, Yamada, Kazunari, additional, Yanagisawa, Yoshiyuki, additional, Yoshida, Atsushi, additional, and Ishihara, Masayasu, additional

Published
2002
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17. Probiotic upregulation of peripheral IL-17 responses does not exacerbate neurological symptoms in experimental autoimmune encephalomyelitis mouse models.

Author

Kobayashi, Toshihide, Suzuki, Tomomi, Kaji, Rumi, Serata, Masaki, Nagata, Tsuyoshi, Ando, Minoru, Iizuka, Ryoko, Tsujibe, Satoshi, Murakami, Junta, Kiyoshima-Shibata, Junko, Kato, Ikuo, Nanno, Masanobu, and Shida, Kan

Subjects
ENCEPHALOMYELITIS, INTERLEUKIN-17, LACTOBACILLUS casei, AUTOIMMUNITY, CENTRAL nervous system, HISTOPATHOLOGY, LABORATORY mice
Abstract

Context: It is of great importance to evaluate the safety of probiotics in dysregulated immune conditions, as probiotics can possibly modulate immune functions in the host. Objective: We tried to confirm the safety of using Lactobacillus casei strain Shirota (LcS) to help prevent autoimmunity in the central nervous system. Methods: We used two chronic experimental autoimmune encephalomyelitis (EAE) models, a relapse and remission type EAE model in SJL/J mice and a durable type model in C57BL/6 mice. LcS was administered from 1 week before antigen sensitization until the end of the experiments, and neurological symptoms and histopathological changes of the spinal cord were observed. Immunological parameters were also examined in the SJL/J mouse model. Results: LcS administration did not exacerbate neurological symptoms or histopathological changes of the spinal cord in either model but instead tended to improve neurological symptoms in the SJL/J mouse EAE model. LcS administration transiently upregulated IL-17 production by antigen-stimulated lymphocytes of draining lymph nodes 7 days after sensitization. Enhanced production of IL-10 and an increase in the percentage of CD4+CD25+ T regulatory cells were also observed at the same sites. Strong expression of IL-17 mRNA was detected in the spinal cord of mice that displayed severe neurological symptoms on day 12, but this expression was not enhanced by LcS administration. Conclusion: These results demonstrate that LcS does not exacerbate, but instead may improve EAE depending on the immunization conditions, and that IL-17 responses at peripheral sites may not always result in a worsening of autoimmune diseases. [ABSTRACT FROM AUTHOR]

Published
2012
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18. Inelastic Scattering of 12Be with 4He

Author

Matsuyama, Yuuichi U., Motobayashi, Tohru, Shimoura, Susumu, Minemura, Toshiyuki, Saito, Akito, Baba, Hidetada, Akiyoshi, Hiromichi, Aoi, Nori, Ando, Yoshiaki, Gomi, Tomoko, Higurashi, Yoshihide, Ieki, Kazuo, Imai, Nobuaki, Iwasa, Naohito, Iwasaki, Hironori, Kanno, Shouko, Kubono, Shigeru, Kunibu, Makoto, Michimasa, Shin'ichiro, Murakami, Hiroyuki, Nakamura, Takashi, Sakurai, Hiroyoshi, Serata, Masaki, Takeshita, Eri, Takeuchi, Satoshi, Teranishi, Takashi, Ue, Koji, Yamada, Kazunari, and Yanagisawa, Yoshiyuki

Abstract

Inelastic scattering of the neutron-rich nucleus 12Be on a liquid-He target has been studied by measuring de-excitation γ rays in coincidence with scattered 12Be. In the angular distribution, a difference between the 2+1 and the 1- state in 12Be was cleary seen.

Published
2013
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19. Coulomb Excitation of 24Si

Author

Kanno, Shouko, Gomi, Tomoko, Motobayashi, Tohru, Yoneda, Ken-ichiro, Aoi, Nori, Ando, Yoshiaki, Baba, Hidetada, Demichi, Kimihiko, Fülöp, Zsolt, Futakami, Udai, Hasegawa, Hirokazu, Higurashi, Yoshihide, Ieki, Kazuo, Imai, Nobuaki, Iwasa, Naohito, Iwasaki, Hironori, Kubo, Toshiyuki, Kubono, Shigeru, Kunibu, Makoto, Matsuyama, Yuuichi U., Michimasa, Shin'ichiro, Minemura, Toshiyuki, Murakami, Hiroyuki, Nakamura, Takashi, Saito, Akito, Sakurai, Hiroyoshi, Serata, Masaki, Shimoura, Susumu, Sugimoto, Takashi, Takeshita, Eri, Takeuchi, Satoshi, Ue, Koji, Yamada, Kazunari, Yanagisawa, Yoshiyuki, Yoshida, Atsushi, and Ishihara, Masayasu

Abstract

We have measured Coulomb excitation of 24Si to its first excited state to obtain the E2 transition probability. This is the first measurement of B(E2) for a TZ = −2 nucleus, which enables us to compare their transition probabilities between the mirror pair of T = 2, namely 24Si and 24Ne.

Published
2013
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20. Molecular States in Neutron-Rich Beryllium Isotopes

Author

Saito, Akito, Shimoura, Susumu, Takeuchi, Satoshi, Motobayashi, Tohru, Akiyoshi, Hiromichi, Ando, Yoshiaki, Aoi, Nori, Fülöp, Zsolt, Gomi, Tomoko, Higurashi, Yoshihide, Hirai, Masaaki, Iwasa, Naohito, Iwasaki, Hironori, Iwata, Yoshiyuki, Kobayashi, Hiroshi, Kurokawa, Meiko, Liu, Zhong, Minemura, Toshiyuki, Ozawa, Shuichi, Sakurai, Hiroyoshi, Serata, Masaki, Teranishi, Takashi, Yamada, Kazunari, Yanagisawa, Yoshiyuki, and Ishihara, Masayasu

Abstract

Excited states in 12Be and 14Be were studied by using an exotic beam of 14Be at 75 A MeV. A new excited state in 12Be was observed at 11.8 MeV. The angular-correlation analysis showed that the spin for the new level is tentatively assigned to be 0. According to the energy-spin systematics, the observed state may be a member of the rotational band of the 6He-6He cluster structure. Three excited states in 14Be were also found at 10.8 MeV, 11.6 MeV, and 15.5 MeV.

Published
2013
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21. Coulomb Dissociation of 23Al

Author

Gomi, Tomoko, Motobayashi, Tohru, Yoneda, Ken-ichiro, Kanno, Shouko, Aoi, Nori, Ando, Yoshiaki, Baba, Hidetada, Demichi, Kimihiko, Fülöp, Zsolt, Futakami, Udai, Hasegawa, Hirokazu, Higurashi, Yoshihide, Ieki, Kazuo, Imai, Nobuaki, Iwasa, Naohito, Iwasaki, Hironori, Kubo, Toshiyuki, Kubono, Shigeru, Kunibu, Makoto, Matsuyama, Yuuichi U., Michimasa, Shin'ichiro, Minemura, Toshiyuki, Murakami, Hiroyuki, Nakamura, Takashi, Saito, Akito, Sakurai, Hiroyoshi, Serata, Masaki, Shimoura, Susumu, Sugimoto, Takashi, Takeshita, Eri, Takeuchi, Satoshi, Ue, Koji, Yamada, Kazunari, Yanagisawa, Yoshiyuki, Yoshida, Atsushi, and Ishihara, Masayasu

Published
2013
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