1. Simultaneous determination of polysaccharides and 21 nucleosides and amino acids in different tissues of <italic>Salvia miltiorrhiza</italic> from different areas by UV–visible spectrophotometry and UHPLC with triple quadrupole MS/MS.
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Xiang, Xiang, Sha, Xiuxiu, Su, Shulan, Zhu, Zhenhua, Guo, Sheng, Yan, Hui, Qian, Dawei, and Duan, Jin‐ao
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POLYSACCHARIDES , *NUCLEOSIDES , *AMINO acids , *TISSUE engineering , *SALVIA miltiorrhiza , *ULTRAVIOLET spectrophotometry , *HIGH performance liquid chromatography , *TANDEM mass spectrometry - Abstract
Abstract:
Salvia miltiorrhiza , a traditional Chinese medicine, is a widely used herbal medicine to treat cardiovascular and cerebrovascular diseases. In this study, ultraviolet (UV)–visible spectrophotometry and ultra‐high performance liquid chromatography with triple quadrupole tandem mass spectrometry analytical methods were used for rapid quantification of polysaccharides and 21 nucleosides and amino acids inS. miltiorrhiza to determine 17 samples of different tissues from different areas. Based on the total contents, hierarchical clustering analysis and principal components analysis were performed to classify these samples. The established methods were validated with good linearity, precision, repeatability, stability, and recovery. Chemical analysis revealed a higher content of total analytes in the sample of inflorescence from Nanjing (34.17 mg/g), sample of root and rhizome from Shaanxi (34.13 mg/g) and sample of stem and leaf from Nanjing (31.14 mg/g), respectively, indicating that root and rhizome from Shaanxi and the aerial parts from Nanjing exhibited the highest quality due to their highest content. In addition, contents of nucleosides and amino acids in the aerial parts (14.67 mg/g) were much higher than that in roots and rhizomes (9.17 mg/g). This study suggested that UV–visible spectrophotometry and ultra‐high performance liquid chromatography with triple quadrupole tandem mass spectrometry are effective techniques to analyze polysaccharides, nucleosides, and amino acids in plants, and they provided valuable information for the development and utilization value of the aerial parts ofS. miltiorrhiza . This analysis would also provide useful information for the quality control ofS. miltiorrhiza . [ABSTRACT FROM AUTHOR]- Published
- 2018
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