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Onion is one of the most popular spice crop, which is used for seasoning wide range of dishes as well as folk medicine. Assessment of genetic diversity helps to use the onion germplasm as breeding materials for its improvement. Molecular marker based diversity assessment is more powerful, rapid and reliable compared to morphology based genetic diversity assessment. Thirty eight onion germplasm were characterized by using 10 random amplied polymorphic DNA (RAPD) markers for estimating genetic diversity within the germplasm. The germplasm exhibited wide range of variability which could be used in cross breeding for getting superior F1 generation. Out of 10 primers, four gave polymorphism among onion germplasm. Genetic diversity of 38 germplasm was analyzed using variation at 34 RAPD loci, of which 91.89% were polymorphic. The highest pair wise genetic distance was 1.046 and the lowest was 0.056. The mean gene diversity among all the germplasm was 1.54 and Shannon's information index was 0.3188. The UPGMA dendrogram, based on Nei`s genetic diversity showed 6 clusters. The frequency of polymorphic loci ranged from 0.0263 to 1.0000, indicates that there exists a wide genetic diversity among the germplasm and corroborated the usefulness of RAPD markers for estimating genetic diversity in onion.

This file contains: Supplementary material & methods Supplementary Figure S1 Globoside GSL synthesizing glycosyltransferases show elevated gene expression in epithelial tissue samples. Supplementary Figure S2 EMT marker gene expression in 47 ovarian cancer cell lines. Supplementary Figure S3 Single cell sorting strategy after transient transfection of sgRNA CRISPR-Cas9 constructs. Supplementary Figure S4 Quantification of glycans released from glycosphingolipids in wildtype and Î"A4GALT ovarian cancer cells using LC-ESI-MS/MS. Supplementary Figure S5 Dissemination of Î"A4GALT compared to IGROV1 wildtype cells. Supplementary Figure S6 The aspartate-any residue-aspartate motif is required for enzymatic activity of A4GALT. Supplementary Figure S7 Bicistronic expression of E-cadherinEGFP and A4GALT DXA shows enhanced extravasation in vivo. Supplementary Figure S8 Genetic depletion of A4GALT correlates with the absence of globosides in ovarian cancer cells representing the four different states of EMT. Supplementary Figure S9 Fluorescently labelled cells were transplanted into the Duct of Cuvier of 48 hpf zebrafish embryos and cells followed for 2-3 days. Supplementary Figure S10 Increased E-cadherin in SKOV3ip coincides with SLUG expression and histone modifications. Supplementary Table S1 Quantification of glycans released from glycosphingolipids in wildtype and Î"A4GALT ovarian cancer cells using LC-ESI-MS/MS. Supplementary Table S2 Cell lines used in this study. Supplementary Table S3 Table providing details of oligonucleotides used in this study. Supplementary references required for supplementary information

Introduction: Coronary angiography is traditionally performed through transfemoral access. Transradial access is increasingly being used for this purpose for its various advantages. However, its use in Bangladesh is less well studied. The aim of this study was to find out the safety and efficacy of transradial access as compared to transfemoral access. Methods: This was a single-center, cross-sectional study. 100 randomly selected elective coronary angiography from August 2017 to September 2018 by the same operator using either transradial access or transfemoral access were analyzed. Results: Among 100 coronary angiography, transradial access were 50 and transfemoral access were 50. Fluoroscopy time was 3.08[1-9] vs 1.47 [1-17] minutes (p=.014), dose area product was 4807 [1947-11489] vs 3202 [1130-12826] ìGy.m2 (P

Background: Atherosclerotic coronary artery disease is rare in young adults aged 40 years or less. However, South Asia is among the top 3 of the countries with highest proportion of cases of first acute coronary syndrome events occurring at age 40 years or less. This study was to explore the extent and pattern of angiographic coronary artery disease among patients of ischemic heart disease aged 40 years or less. Methods: This cross-sectional retrospective descriptive study included 140 patients who had coronary angiography for ischemic heart disease at the Department of Cardiology of Enam Medical College between July 2012 and December 2020. Coronary angiography was analyzed with particular attention to the extent of involvement of major epicardial coronary arteries including significant branch involvement. Results: Of the 140 patients included in the study - aged 18-40 years (mean 35.90 and SD 4.05) and 59.28% in the 36-40 years age group – 85% were male. Coronary angiogram revealed Single Vessel Disease in 39.29%, Double Vessel Disease in 25.72%, Triple Vessel disease in 23.57%, normal Coronaries in 9.28%. Left Anterior descending territory was most involved - 94 (67.14%) patients having significant stenosis in the main trunk and/or one or more major branches. Overall, 23(16.43%) of the whole cohort of patients had multiple branch involvements in the three coronary artery territories. Conclusion: In patients aged up to 40 years with ischemic heart disease, significant coronary artery disease burden was seen, including a significant proportion with multi-vessel disease and a high level of branch involvement in all the principal epicardial coronary territories. This indicates a high burden of disease in a relatively young age group, with significant implications for long term management over and beyond reperfusion/revascularization efforts. Cardiovasc j 2022; 14(2): 103-110

Onion is an inevitable part of our food habits. Fungal infection is one of the major reasons of onion spoilage which not only cause economic losses but also may cause public health threat through mycoses and mycotoxicoses. A total 15 onion samples from different places in Chattagram, Bangladesh were collected to assess the fungal contaminants.Onion consumers along with retailers were interviewed to evaluate their awareness about fungal food spoilage and associated health risk. Three different fungal species were identified and isolated by analysing their macroscopic and microscopic features. These isolates were Aspergillus niger, Aspergillus flavus, and Penicillium spp. A. niger was the most prevalent and found in 14 samples out of 15. Biochemical characterization of the isolated fungi was also done to assess their ability to produce extracellular enzymes and amylase, protease, and cellulase activities were observed. Survey data showed that only around 20% of the interviewees had some idea about fungal contamination, while nearly about 80% of them believed that washing, sunburn and cooking can make the food safe. Bioresearch Commu. 8(1): 1068-1076, 2022 (January)

Farmers in the Bogura region benefit significantly from the systematic use of crop rotation. The experiment was performed at the Shibganj, Bogura, in 2015-16 and 2016-17 to create a potato-Boro-radish-T. Aman rice crop sequence that would compete with the potato-Boro-T. Aman rice crop sequence. Primary objectives of the experiment were to determine the cost of producing enhanced Potato-Boro-Radish-T. Aman rice and compare it to current potato-boro-t. aman rice. There were two patterns: Current Cropping Pattern Potato (BARI Alu-8)-Boro (BRRI dhan 28)-T. Aman (BRRI dhan-49) and Alternative Cropping Pattern Potato (BARI Alu-25)-Boro (BRRI dhan 28) -Radish (Rocky)-T. Aman (BRRI dhan-57). The research revealed that an alternative cropping pattern required average crop duration of 315 days to complete one cycle in a year, implying that a four multiple cropping sequence was economically viable to replace the current cropping pattern. In terms of mean REY, the total grain/tuber yield was 42.80 t/ha/year, which was 66% greater than the current pattern (25.76 t/ha/year). The overall production efficiency (185.14 kg/ha/day), usage of land (86.16%), and engagement in the population (660 man-days/ha/year) of the alternative cropping pattern were 45, 7, and 41% higher, respectively, than the current cropping pattern. Alternative cropping patterns had the gross benefit of 274911 Tk./ha/year, indicating that they were sparingly sustainable. The alternative crop sequence also improved crop strength, growers' real understanding, technique, profits, and engagement. Furthermore, by trying to incorporate T. aman straw into soil with an alternative cropping pattern, it promotes soil health. As a result, farmers in Bangladesh's Bogura region may employ a different planting pattern on their variable land in order to boost production and profitability while also creating jobs.

Background: Escherichia coli is a common causative of blood stream infection having potentials to produce significant morbidity and mortality. This organism also has the ability to develop resistance against antimicrobial agents. Knowing its epidemiology, risk factors and antimicrobial resistance patterns can help preventing and managing bacteremia caused by this organism. Materials and methods: This was across sectional observational study carried out from February 2017 to February 2018 on 64 blood culture positive Escherichia coli infected patients admitted in Medicine inpatient of a medical college hospital. Age, sex, mode of acquisition of infection, history of prior empiric antibiotic treatment, duration of hospital stay, development of complication were observed and noted. Antibiotic susceptibility test for all isolates was performed by Kirby-Bauer disc diffusion method. Predesigned semi-structured data collection from was used and collected data were analyzed manually and expressed in descriptive statistical terms. Results: Of the 64 enrolled patients, 47(73.43%) were female. Average age of affection was 53.48±20.65 years and increased incidence rates (51.56%) was observed at age >60 years. Infection was communityacquired in 35.84% cases and urinary tract infection was the most frequent (46.3) risk factor. More than eighty seven percent of samples showed resistance to at least one antimicrobial agent and resistance to multiple drugs was associated with complications. Conclusion: Escherichia coli bacteremia has high incidence rates for antimicrobial resistance and mortality. Continuous surveillance and antibiotic susceptibility pattern monitoring is essential to develop regional antibiotic therapy protocols.

The physico-chemical interaction, surface, and electrical properties of hole transport layers (HTLs) significantly affect the wettability and film crystallization of the deposited perovskite and the corresponding performance of inverted perovskite solar cells (PSCs). One of the most frequently used HTLs for inverted PSCs is poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS). Various commercial PEDOT:PSS formulations have already been tested as HTLs. Until now mixtures of these remained rather unexplored. In this study, three commercially available PEDOT:PSS formulations (PH, PH1000, and AI4083), as well as three mixtures (PH:PH1000, PH:AI4083, and PH:PH1000:AI4083; in a 1 : 1 and 1 : 1 : 1 ratios) were used as HTLs to investigate the crystallization of perovskite films and the performance of associated PSCs. Of the three formulations, PEDOT:PSS PH showed better perovskite crystallization, resulting in better solar cell performance followed by both PH:AI4083 and PH:PH1000:AI4083 layers. The pioneering work on mixing PEDOT:PSS resulted in new combinations of PEDOT:PSS, with new properties (work function, surface wettability, and roughness) which are very important parameters for perovskite crystallization and corresponding device efficiencies and stabilities. All PSCs that use the mixed PEDOT:PSS as HTLs revealed higher fill factor and open-circuit voltage values above 900 mV, which is not the same except for PEDOT:PSS PH. As a result, we believe that such a mixing strategy could aid in the creation of various PEDOT:PSS combinations with tuneable properties for certain applications.

Poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) is up to date the most popular and commercially most successful conductive polymer. It is being used not only for antistatic, anti...

It is common knowledge that molecular energy level offsets of a type II heterojunction formed at the donor–acceptor interface are considered to be the driving force for photoinduced charge transfer in organic solar cells. Usually, these offsets – present between molecular energy levels of the donor and acceptor – are obtained via cyclic voltammetry (CV) measurements of organic semiconductors cast in a film or dissolved in solution. Simply transferring such determined energy levels from solution or film of single materials to blend films may be obviously limited and not be possible in full generality. Herein, we report various cases of material combinations in which novel non-fullerene acceptors did not yield successful charge transfer, although energy levels obtained by CV on constituting single materials indicate a type II heterojunction. Whilst the integer charge transfer (ICT) model provides one explanation for a relative rise of molecular energy levels of acceptors, further details and other cases have not been studied so far in great detail. By applying energy-resolved electrochemical impedance spectroscopy (ER-EIS) on several donor–acceptor combinations, a Fano-like resonance feature associated with a distinctive molecular energy level of the acceptor as well as various relative molecular energy level shifts of different kinds could be observed. By analyzing ER-EIS and absorption spectra, not only the exciton binding energy within single materials could be determined, but also the commonly unknown binding energy of the CT state with regard to the joint density of states (jDOS) of the effective semiconductor. The latter is defined by transitions between the highest occupied molecular orbitals (HOMO) of the donor and the lowest unoccupied molecular orbitals (LUMO) of the acceptor. Using this technique among others, we identified cases in which charge generation may occur either via uphill or by downhill processes between the charge transfer exciton and the electronic gap of the effective semiconductor. Exceptionally high CT-exciton binding energies and thus low charge generation yields were obtained for a case in which the donor and acceptor yielded a too intimate blend morphology, indicating π–π stacking as a potential cause for unfavorable molecular energy level alignment.

Background: Groin hernias are the most common surgical conditions all over the world. Laparoscopic (TAPP) approach has been achieved as the best technique of inguinal hernia repair due to less postoperative pain, earlier return to activity and fewer wound complications. Objectives: To share our experience with Transabdominal preperitoneal (TAPP) hernia repair and its outcome. Methods: This prospective study was performed in Shaheed Suhrawardy Medical College Hospital between January 2016 to December 2017. Forty male patients with inguinal hernia were included in this study. Data was collected in a proforma regarding demographic and baseline characteristics, operative time, outcome of patients and were analyzed in tabulated form. Results: Total 40 patients were included in our study. Age ranged from 20-60 years (mean age ± SD 38.88±10.92 years). The maximum duration of TAPP hernia repair ranged from 100-120 minutes s(mean ± SD 109±16.3 minutes). Post-operative complications were severe pain (10%), infection (5%), scrotal oedema (5%). Conclusion: Laparoscopic TAPP hernia repair is associated with satisfactory short- and long-term results. Journal of Surgical Sciences (2020) Vol. 24 (1) : 11-14

The Constitutions of democratic countries list, and elaborately specify the makeup and functions of, three branches of government: the Executive, Legislature, and Judiciary. The Media, dubbed the Fourth Estate by Edmund Burke, cannot be a part of the government. In fact, to contemplate a free media being a part of a government would be an oxymoron. After all, the Media is there to keep a check on the abuse of power by the government and other power structures. This paper looks at the media’s role in a country’s governance, security, and development, and these are encapsulated, indicating also to their complementarity, in the quality traditional media’s glamour best: political journalism. Today, in the early twenty-first century, journalism is still, for good and ill, at the heart of politics. But political journalism is also changing and reinventing itself as a craft and a profession in the face of harsh competition, a rapidly changing business environment, and a political world undergoing its own profound changes. Furthermore, in the Internet Age, the new media, because of the possibilities for good or mischief that it can create, is often a target for manipulation towards one’s own benefit, and at the expense of other political entities, in a number of countries.

Background: Inguinal hernia repair is a commonly performed general surgical procedure that constitute more than 95% of all groin hernia repairs. However, in developing countries, quite a considerable percentage of it is not repaired or delayed repaired and that lead to a higher incidence of morbidity and mortality. so, we planned to conduct this study to understand the clinic-epidemiologic profile of inguinal hernia in a tertiary care hospital of Bangladesh. Objective: To evaluate the clinical, epidemiological profile and associated risk factors for inguinal hernia. Methodology: This observational study was conducted among 100 patients during January 2018 to January 2019 who admitted in the surgery department of a tertiary care center for inguinal hernia surgery . All the study subjects were examined and their clinical and epidemiological profiles studied, tabulated and analyzed. Result: Among the 100 patients, most of them (94%) were men with a age of 57.02 ± 12.87, farmer (36%) by occupation.61% patients were in low saocio-ecenomic status. Most of the patients (60%) were in the age group of 40-60 years followed by less than 30 years (21%). On query regarding symptoms of inguinal hernia, all of them (100%) complaints of groin swelling. More than half of the patients complaints of groin pain and sensation of heaviness in groin. On clinical examination, right sided, left sided, bilateral hernias were found in 49%,45% and 6% study patients respectively. Direct hernias, indirect, pantaloons hernias were seen in57%,30%,7% study patient respectly. Most of hernias (81%) were reducible and were incomplete (88%). Most of the patients (75%) presented late to the health care center due to the lack of awareness of the disease and were initially treated by homeopath medicine. During query about risk factors for inguinal hernia,46% patients were more than 50 years of age and 26% patients were smoker. In this study, most common hernia repair procedure was Lichtenstein’s procedure (63%) followed by modified Bassini’s procedure (11%). Total open procedure were 93% and laparoscopic hernia repairs were done only in 7% patients.

Background: Fistula in ano of complex variety has been a common surgical problem. Varieties of surgical procedures are encountered for management of this disease but recurrence is a notorious complication of this disease. Objective: The purpose of the present study was to assess outcomes of complex fistula in ano after fistulotomy with seton procedure. Methodology: This prospective study was performed in Dhaka Dental College, Dhaka, Bangladesh from January 2017 to December 2018 for a period of two years. Patients were included in this study. Data collected in data collection sheet regarding demographic data, types of operative procedures, post-operative complications and outcome of patients which were then analyzed. Result: Total 31 patients were included in this study. Age ranged from 20 to 60 years. Male was 28(90.32%) cases and female was in 3(9.68%) cases. High trans-sphincteric fistula were 28(90.32%) cases, extra-sphincteric fistula were 3(9.68%) cases. Fistulotomy with cutting seton by rubber bands were 12(38.71%) cases, fistulotomy with cutting seton by silk 19(61.29%) cases. Seton fell on its own 16(51.61%) cases, not fell 15(48.39%). Post-operative complications were incontinence of gas 5(16.13%) cases, incontinence of stool 1(3.23%) cases, recurrence 1(3.23%) cases. Conclusion: Outcomes of fistulotomy with cutting seton were satisfactory in most patients. Early recovery, low complication rate were encountered in treatment of complex fistula in ano by partial fistulotomy with cutting seton procedure. Journal of Current and Advance Medical Research 2020;7(1): 40-43

Background: Inguinal hernia repair is very common in day to day general surgical practice. Result of surgical repair is often satisfactory but recurrences following surgery are troublesome both for the surgeon and the patient. Lichtenstein technique is now the most widely performed technique in groin hernia repair. Objective: The aim of this study was to assess short term outcome of complete variety of inguinal hernia repair by Lichtenstein technique. Methodology: This was a prospective observational study in which 30 complete variety of inguinal hernia repairs were performed by Lichtenstein technique between January 2014 and December 2017 by same surgical team in surgery department, Holy Family Red Crescent Medical College Hospital (HFRCMCH). Patients were scheduled for follow up visits at the end of first week, third month and sixth month after operation in out-patient department. The main outcome measures were postoperative complications, early recurrence, groin pain. Result: In this study age of the patients ranged from 30 years to 78 years, the mean age was 51.93 (±SD 10.12) years. Most of the hernia were of indirect type 18(60%) followed by direct type 9(30%). In indirect type (55.6%) of the hernias was right sided and the rest (44.4%) were left sided. On an average each operation lasted for 1.18 hours and oral feeding started 12.69 hours after the operation, Postoperative mean hospital stay that was 2.27 days. Postoperative complications were 2(6.7%) had a prolonged recovery and presented with abdominal distention after operation and 3(10%) patients developed postoperative urinary retention. Scrotal haematoma formation was observed in 2(6.7%) cases and other post-operative complications were local haematoma or seroma formation in incision site, periincisional skin echymosis, postoperative pyrexia, superficial wound infection and post-operative pain were found in 1 (3.3%) cases. Regarding outcome no recurrence or mesh rejection or mesh infection were observed within short time postoperative follow up period. Conclusion: In this study no recurrence or mesh rejection or mesh infection were observed within short time postoperative follow up period and patient's compliance was good with minimum morbidity. Therefore, Lichtenstein's technique of inguinal hernia repair considered as a safe and effective procedure. Northern International Medical College Journal Vol.10 (2) Jan 2019: 389-392

The present study investigated the length-weight relationship, population parameters and the instantaneous natural mortality rate of Arius thalassinus from two major landing center of Bangladesh using the FAO-ICLARM Stock Assessment Tools (FiSAT II). A total of 1789 specimens were collected between January 2016 and December 2017. The estimated values of the exponent “b” was ranged from 3.019 (pre-monsoon) to 3.293 (monsoon), indicating an isometric growth pattern of the species with high correlation coefficients (0.904 to 0.927). The equation of length–weight relationship was: W=0.005L3.181. Model parameters of the von Bertalanffy growth equation were: L∞ = 97.60 cm and K = 0.33-1 year. The estimated growth performance index (Ø) was 3.497. The instantaneous natural mortality rate (M) was 0.62-1 year and a high exploitation rate (E) 0.62 (>0.50) showed that this fish is over-exploited in the Bay of Bengal coast of Bangladesh. Res. Agric., Livest. Fish.6(3): 439-444, December 2019

The molecular repertoire promoting cancer cell plasticity is not fully elucidated. Here, we propose that glycosphingolipids (GSLs), specifically the globo and ganglio series, correlate and promote the transition between epithelial and mesenchymal cells. The epithelial character of ovarian cancer remains stable throughout disease progression, and spatial glycosphingolipidomics reveals elevated globosides in the tumor compartment compared with the ganglioside-rich stroma. CRISPR-Cas9 knockin mediated truncation of endogenous E-cadherin induces epithelial-to-mesenchymal transition (EMT) and decreases globosides. The transcriptomics analysis identifies the ganglioside-synthesizing enzyme ST8SIA1 to be consistently elevated in mesenchymal-like samples, predicting poor outcome. Subsequent deletion of ST8SIA1 induces epithelial cell features through mTOR

et al., Large datasets are now ubiquitous as technology enables higher-throughput experiments, but rarely can a research field truly benefit from the research data generated due to inconsistent formatting, undocumented storage or improper dissemination. Here we extract all the meaningful device data from peer-reviewed papers on metal-halide perovskite solar cells published so far and make them available in a database. We collect data from over 42,400 photovoltaic devices with up to 100 parameters per device. We then develop open-source and accessible procedures to analyse the data, providing examples of insights that can be gleaned from the analysis of a large dataset. The database, graphics and analysis tools are made available to the community and will continue to evolve as an open-source initiative. This approach of extensively capturing the progress of an entire field, including sorting, interactive exploration and graphical representation of the data, will be applicable to many fields in materials science, engineering and biosciences., Open access funding provided by Helmholtz-Zentrum Berlin für Materialien und Energie GmbH., The core funding of the project has been received from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 787289. We acknowledge the following sources for individual funding. Cambridge India Ramanujan Scholarship, China Scholarship Council, Deutscher Akademischer Austauschdienst (DAAD), EPSRC (grant no. EP/S009213/1), European Union’s Horizon 2020 research and innovation programme (grant no. 764787, EU Project ‘MAESTRO’), (grant no. 756962, ERC Project ‘HYPERION’), (grant no. 764047, EU Project ‘ESPResSo’ and grant no. 850937), GCRF/EPSRC SUNRISE (EP/P032591/1), German Federal Ministry for Education and Research (BMBF), HyPerFORME, NanoMatFutur (grant no. 03XP0091). PEROSEED (ZT-0024), Helmholtz Energy Materials Foundry, The Helmholtz Innovation Laboratory HySPRINT. BMBF (grant nos. 03SF0540, 03SF0557A), HyPerCells graduate school, Helmholtz Association, Helmholtz International Research School (HI-SCORE), the Erasmus programme (CDT-PV, grant no. EP/L01551X/1), the European Union’s Horizon 2020 research and innovation programme (Marie Skłodowska-Curie grant agreement nos. 841386, 795079 and 840751), Royal Society University Research Fellowship (grant no. UF150033). SNaPSHoTs (BMBF), SPARC II, German Research Foundation (DFG, grant no. SPP2196), The National Natural Science Foundation of China (grant no. 51872014), the Recruitment Programme of Global Experts, Fundamental Research Funds for the Central Universities and the ‘111’ project (grant no. B17002), the US Department of Energy’s Office of Energy Efficiency and Renewable Energy under Solar Energy Technologies Office (SETO) agreement no. DE-EE0008551, the Colombia Scientific Programme in the framework of the call Ecosistema Cientifíco (Contract no. FP44842-218-2018), the committee for the development of research (CODI) of the Universidad de Antioquia (grant no. 2017-16000), Spanish MINECO (Severo Ochoa programme, grant no. SEV‐2015‐0522), the Swedish research council (VR, grant no. 2019-05591) and the Swedish Energy Agency (grant no. 2020-005194).

Atomic force microscopy (AFM) is the only technique that allows label-free imaging of nanoscale biomolecular dynamics, playing a crucial role in solving biological questions that cannot be addressed by other major bioimaging tools (fluorescence or electron microscopy). However, such imaging is possible only for systems either extracted from cells or reconstructed on solid substrates. Thus, nanodynamics inside living cells largely remain inaccessible with the current nanoimaging techniques. Here, we overcome this limitation by nanoendoscopy-AFM, where a needle-like nanoprobe is inserted into a living cell, presenting actin fiber three-dimensional (3D) maps, and 2D nanodynamics of the membrane inner scaffold, resulting in undetectable changes in cell viability. Unlike previous AFM methods, the nanoprobe directly accesses the target intracellular components, exploiting all the AFM capabilities, such as high-resolution imaging, nanomechanical mapping, and molecular recognition. These features should greatly expand the range of intracellular structures observable in living cells.

The black pomfret (Parastromateus niger) is one of the high-income-generating carangid fish species and the only known member of its genus. This study aims to identify existing gaps in stock status and population demography of this species and provide viable management recommendations to enhance the sustainability of this fishery. Therefore, three methodological approaches have been used in this study; TropFishR to present the current exploitation status, the length-based spawning potential ratio (LB-SPR) to quantify stock spawning biomass, and Froese’s sustainability indicators (LBI) to establish a basic standard for sustainable management of the fishery. In the length-weight relationship of black pomfret, an allometric growth pattern (b = 2.19) was found. The VBGF life history parameters for black pomfret were L∞ = 55.25 cm, K = 0.54 year−1, and based on LCCC analysis, the total mortality (Z = 1.61 year−1), natural mortality (M = 0.69 year−1) and fishing mortality (F = 0.91 year−1) are calculated. The result reveals that this fishery is currently suffering from overexploitation and the stock’s spawning biomass (SPR = 13%) is below the limit reference point because most of the catch (82%) was found to be under the maturity level. Based on the results, this study recommended strictly maintaining the mesh size of the net to ensure not to catch immature fish with a length smaller than 30.63 cm, recommended the length to catch be between 29 and 35 cm, and reducing fishing pressure by one-third to ensure the sustainability of the black pomfret fishery.

G protein-coupled receptors (GPCRs) are one of the most important drug-target classes in pharmaceutical industry. Their diversity in signaling, which can be modulated with drugs, permits the design of more effective and better-tolerated therapeutics. In this work, we have used rigid oligoproline backbones to generate bivalent ligands for the gastrin-releasing peptide receptor (GRPR) with a fixed distance between their recognition motifs. This allows the stabilization of GPCR dimers irrespective of their physiological occurrence and relevance, thus expanding the space for medicinal chemistry. Specifically, we observed that compounds presenting agonists or antagonists at 20- and 30-A distance induce GRPR dimerization. Furthermore, we found that 1) compounds with two agonists at 20- and 30-angstrom distance that induce dimer formation show bias toward Gq efficacy, 2) dimers with 20- and 30-angstrom distance have different potencies toward beta-arrestin-1 and beta-arrestin-2, and 3) the divalent agonistic ligand with 10-angstrom distance specifically reduces Gq potency without affecting beta-arrestin recruitment, pointing toward an allosteric effect. In summary, we show that rigid oligoproline backbones represent a tool to develop ligands with biased GPCR signaling., Proceedings of the National Academy of Sciences of the United States of America, 118 (48), ISSN:0027-8424, ISSN:1091-6490

Background: Sacrococcygeal pilonidal sinus is a common morbid disease in young adult patients with high recurrence rate. There are several surgical procedures for managing pilonidal sinus. The present study aims to evaluate the efficacy of limberg flap reconstruction surgery for the management of sacrococcygeal pilonidal sinus. Methods: A prospective study was conducted over a period of more than two years from January 2017 to June 2019. A total of 21 patients were included in this study. All patients underwent Limberg flap reconstruction. Data collected in data collection sheet regarding demographic data, post operative complications and analyzed in tabulated form. Results: Total 21 patients were included in our study. Age ranged from 20-50 years. Male were 18(85.71%) and female were 3(14.29%). Post operative complications were seroma 2(9.52%), wound infection 1(4.76%). But no flap necrosis and recurrence had occurred. Conclusion: Limberg flap for reconstruction of the defect after excision of sacroccoccygeal pilonidal sinus is an effective and safe technique with low complication rate. J Dhaka Medical College, Vol. 29, No.1, April, 2020, Page 38-40

Background: A breast lump is the most common symptom associated with both benign and malignant breast diseases. Therefore, a distinction of benign from malignant lump is of importance for proper management. Though a definitive diagnosis is possible with imaging for all the lesions, histopathological study is proven essential for confirming the diagnosis. Objective: The objective of this study was to evaluate the role of USG and histopathological findings of different breast lump in diagnosis and their comparison. Methods: A prospective study was conducted over a period of one year from January 2017 to December 2017. A total of 116 patients were included in this study. All breast lumps underwent surgery and the ultrasound findings of these lumps were compared with the histopathological findings. Data were collected from these patients by a preformed questionnaire and finally the data were analyzed. Results: Out of 116 patients only 21 cases were reported as malignant in ultrasound report but histopathology revealed 31 malignant patients. On histopathological examination 10 benign cases turned out to be malignant. Conclusion: The present study was undertaken to evaluate in diagnosing breast mass lesions individually by ultrasound and compared with histopathology for definitive management of a patient. Journal of Surgical Sciences (2019) Vol. 23 (1) : 10-12

A compact microstrip-fed antenna consisting of radiating rings and parasitic strips is proposed for frequency- and pattern-reconfigurability. The 24.7% frequency tuning range is achieved by controlling the input impedance at the rings using two varactor diodes. By regulating the radiation from the rings and changing the role of the strips from reflector to director (vice versa), nearly 360° azimuth beam-scanning is realized. A prototype is built and tested to validate the concept. Despite having a compact size and thin profile ( ${\text{0.27}}\lambda _{0}\times {\text{0.34}}\lambda _{0}\times {\text{0.005}}\lambda _{0}$ , $\lambda _{0}$ at the lowest frequency), the antenna with its simplified mechanism possesses multiple functions that are comparable with array antennas for similar purposes.

Current–voltage ( IV) characterization is the most fundamental measurement performed on solar cells. This measurement is commonly used to extract basic solar cell parameters, such as open circuit voltage, short circuit current density, fill factor, and power conversion efficiency. We were able to obtain a fast tool to find defective behavior using Simulation Program with Integrated Circuit Emphasis simulations and generate an understanding of which device property can create such defective behaviors by analyzing the second derivative of IV curves.

Fixed performing antennas are ineffective in providing optimum performance in a changing multipath environment, such as underground mines where channel conditions quickly change as the antenna is moved. Hence, we investigated the performance of a pattern reconfigurable antenna (RA) in improving the wireless communications link in a hard rock underground mine. An RA and a dipole were characterized and compared in line-of-sight (LOS) and nonline-of-sight (NLOS) propagation scenarios. As a proof of concept, experiments were performed at the University of Queensland Experimental Mine using a 1 GHz frequency band centered at 2.45 GHz. Analysis of complex channel impulses captured through the frequency-domain channel sounding is presented. Also, the results show that reconfigurability in the rich multipath environment achieves a 20% and 34% improvement in path loss and delay spread, respectively, compared to a fixed-beam antenna in NLOS propagation. Furthermore, more than 34% improvement in coherence bandwidth is observed in LOS and NLOS. Therefore, reliable high-data-rate communication is achievable in underground mines by the effective management of multipath effects using the RA.

The study aimed to select high-yielding, farmers-preferred quality sweet potato varieties that are suitable to grow in various environmental conditions in Bangladesh. In this context, four popular sweet potato varieties (viz., ‘BARI Mistialu-8′, ‘BARI Mistialu-12′, ‘BARI Mistialu-14′, and ‘BARI Mistialu-15′) were used in the study. These varieties were released by Bangladesh Agricultural Research Institute (BARI). In the first season (2018–2019), these varieties were evaluated at nine locations, and in the second season (2019–2020), the same varieties were tested further, but only in three locations. The trial was set up in a randomized complete block design and repeated three times. After two years of observation, it was found that the fresh root yield was varied significantly due to the environment (E), genotypes (G), and their (G × E) interaction (p ≤ 0.01) by using genotype and genotype x environment (GGE) biplot analysis. The E and G × E interaction effects were found to the greater than the genotypes effect solely. In the first year, three varieties, namely ‘BARI Mistialu-8′, ‘BARI Mistialu-12′ and ‘BARI Mistialu-14′, were identified as balanced and comparatively higher in yield in nine locations. These three varieties also showed a similar trend with respect to root yield in tested three locations in the second year. Among the four varieties, ‘BARI Mistialu-12′ was found to be the highest root yielder, followed by ‘BARI Mistialu-8′ and ‘BARI Mistialu-14′. Across the locations, these varieties showed 57.89%, 61.50% and 44.30% higher yield than the local check cultivar. Therefore, these three varieties may be recommended as the best varieties of sweet potato throughout the country.

The anadromous tropical Hilsa shad formed the largest single-species fishery in Bangladesh, making the highest contribution to the country’s total fish production (14%) and nearly 83% of the global Hilsa catch in 2018. However, increased fishing pressure made the fishery vulnerable, and hence, information on the stock condition and its response to the current degree of removal is essential to explore the future potential for sustainable exploitation. This study carried out a rigorous assessment based on three different methodological approaches (traditional length-frequency based stock assessment method for fishing mortality and exploitation, Froese’s length-based indicators for fishing sustainability, and a surplus production-based Monte Carlo method-CMSY, for fisheries reference points estimation) for the best possible estimates of the Hilsa stock status in the water of Bangladesh. The present findings revealed that the stock is likely to be overfished due to over-exploitation. Depending on the outputs, this study recommended a lower length limit for the catch (&gt, 33 cm), distinguished a selectivity pattern (mesh size limit ≥ 8 cm), and proposed a yearly landing limit (within the range of 263,000–315,000 tons) for the sustainable management of the Hilsa fishery in Bangladesh.