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3 results on '"Shahla Asgharzadeh Kangachar"'

1. Comparative Investigation of Bachelor’s Degree Curriculum of Clinical Laboratory Sciences in Iranian Universities of Medical Sciences and World\'s Top Universities

Author

Shahla Asgharzadeh kangachar, Ali Mojtahedi, Rasoul Tabari, and Iraj Nikokar

Subjects
Clinical Laboratory Sciences, Pedagogical Curriculum, Iran and world Universities, Microbiology, QR1-502
Abstract

Background and Aim: Nowadays, clinical laboratories play important roles in health care systems and well-trained staff are in need more than ever before. Thus, educating students in this field is one of the best approaches in saving energy and expenditure of health-care systems in any community. The aim of this descriptive study was to assess the different aspects of the pedagogical curriculum for Iranian laboratory science students compared with that of world's leading universities. Materials and Methods: In 2014, pedagogical curriculum of this major in Iran was compared to the curriculum of 17 prestigious universities in the world. Also, a questionnaire was filled out by the faculty members of medical universities who were involved in training clinical laboratory science's students. Results: The results showed that the Iranian curriculum has various drawbacks in clinical apprenticeship for students, and is short of specific credits such as Microbiology and Immunohematology. In addition, lack of practical or applied credits like Urine Analysis, Body Fluids, Microscopy and Medical Ethics was noticed. It was discovered that little attention was paid to preparing students for research. Conclusions: Considering the findings of the current survey, decreasing the credits offered in each semester with proper training course scheduling and monitoring the students, will not only help the students to proper learning, but also lead to the delivery of other goals. Also, due to the significant role of these students in the healthcare system, it is necessary to hold practical and theoretical exams of this course before entrance of students to the labor market in order to prevent the probable detrimental impacts on the healthcare system.

Published
2016

2. A bacterial genome assembly and annotation laboratory using a virtual machine

Author

Ellina Trofimova, Shahla Asgharzadeh Kangachar, Karen D. Weynberg, Robert Willows, and Paul Jaschke

Subjects
Molecular Biology, Biochemistry
Abstract

With the global increase of infections caused by antibiotic-resistant bacterial strains, there is an urgent need for new methods of tackling the issue. Genomic analysis of bacterial strains can help to understand their virulence and antibiotic resistance profile. Bioinformatic skills are in great demand across the biological sciences. We designed a workshop that allows university students to learn the process of genome assembly using command line tools within a virtual machine on a Linux operating system. We use Illumina and Nanopore short and long-read raw sequences to reveal the advantages and disadvantages of short, long, and hybrid assembly methods. The workshop teaches how to assess read and assembly quality, perform genome annotation, and analyse pathogenicity, antibiotic, and phage resistance. The workshop is intended for a five-week teaching period and is concluded by a student poster presentation assessment.

Published
2023

3. Evaluation of integrons classes 1–3 in extended spectrum beta-lactamases and multi drug resistant Escherichia coli isolates in the North of Iran

Author

Shahla Asgharzadeh kangachar and Ali Mojtahedi

Subjects
0301 basic medicine, Transposable element, biology, medicine.drug_class, 030106 microbiology, Antibiotics, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, medicine.disease_cause, law.invention, Microbiology, 03 medical and health sciences, 030104 developmental biology, Antibiotic resistance, law, Genetics, medicine, bacteria, Agar diffusion test, Escherichia coli, Prophage, Bacteria, Polymerase chain reaction
Abstract

Antimicrobial resistance of Escherichia coli is an increasing problem that makes treatment complicated. Integrons may help bacteria to acquire novel combinations of resistance genes which can play an important role in antibiotic resistance. This study aimed to determine the prevalence of class I-III integrons in clinical isolates of E. coli and their probable relation to multi-drug resistant (MDR) and extended-spectrum beta-lactamases (ESBLs). During the eight months from November 2014 to July 2015, plasmid DNA of 193 E. coli isolates was extracted after determining their resistance pattern and ESBL production by disk diffusion method. To detect the presence of class I–III integrons polymerase chain reaction (PCR) was performed for all isolates. Among 193 isolates, 37.3% of isolates were ESBL-producing. Also, 79.3% and 13% were positive for intl I and intl II genes, respectively; while intl III gene was not detected. Overall, 65.9% and 81.8% of isolates were MDR which isolated from urinary and non-urinary samples respectively. Our results indicated a negative association between the presence of class I and II integrons and resistance to some of the studied antibiotics. The contrary results may be due to empty or nonfunctional integrons or presence of gene cassettes on prophages or transposons instead of integrons.

Published
2018

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