Search

Your search keyword '"Sham, Kendig"' showing total 13 results
Start Over Author "Sham, Kendig"
13 results on '"Sham, Kendig"'

1. Adaptation to ex vivo culture reduces human hematopoietic stem cell activity independently of the cell cycle

Author

Johnson, Carys S., Williams, Matthew, Sham, Kendig, Belluschi, Serena, Ma, Wenjuan, Wang, Xiaonan, Lau, Winnie W. Y., Kaufmann, Kerstin B., Krivdova, Gabriela, Calderbank, Emily F., Mende, Nicole, McLeod, Jessica, Mantica, Giovanna, Li, Juan, Grey-Wilson, Charlotte, Drakopoulos, Michael, Basheer, Shaaezmeen, Sinha, Shubhankar, Diamanti, Evangelia, Basford, Christina, Wilson, Nicola K., Howe, Steven J., Dick, John E., Göttgens, Berthold, Green, Anthony R., Francis, Natalie, and Laurenti, Elisa

Published
2024
Full Text
View/download PDF

2. STAT1 is essential for HSC function and maintains MHCIIhi stem cells that resist myeloablation and neoplastic expansion

Author

Li, Juan, Williams, Matthew J., Park, Hyun Jung, Bastos, Hugo P., Wang, Xiaonan, Prins, Daniel, Wilson, Nicola K., Johnson, Carys, Sham, Kendig, Wantoch, Michelle, Watcham, Sam, Kinston, Sarah J., Pask, Dean C., Hamilton, Tina L., Sneade, Rachel, Waller, Amie K., Ghevaert, Cedric, Vassiliou, George S., Laurenti, Elisa, Kent, David G., Göttgens, Berthold, and Green, Anthony R.

Published
2022
Full Text
View/download PDF

3. Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Author

Mende, Nicole, Bastos, Hugo P., Santoro, Antonella, Mahbubani, Krishnaa T., Ciaurro, Valerio, Calderbank, Emily F., Quiroga Londoño, Mariana, Sham, Kendig, Mantica, Giovanna, Morishima, Tatsuya, Mitchell, Emily, Lidonnici, Maria Rosa, Meier-Abt, Fabienne, Hayler, Daniel, Jardine, Laura, Curd, Abbie, Haniffa, Muzlifah, Ferrari, Giuliana, Takizawa, Hitoshi, Wilson, Nicola K., Göttgens, Berthold, Saeb-Parsy, Kourosh, Frontini, Mattia, and Laurenti, Elisa

Published
2022
Full Text
View/download PDF

6. Adaptation toex vivoculture drives human haematopoietic stem cell loss of repopulation capacity in a cell cycle independent manner

Author

Johnson, Carys S., primary, Sham, Kendig, additional, Belluschi, Serena, additional, Wang, Xiaonan, additional, Lau, Winnie, additional, Kaufmann, Kerstin B., additional, Krivdova, Gabriela, additional, Calderbank, Emily F., additional, Mende, Nicole, additional, McLeod, Jessica, additional, Mantica, Giovanna, additional, Williams, Matthew J., additional, Grey-Wilson, Charlotte, additional, Drakopoulos, Michael, additional, Sinha, Shubhankar, additional, Diamanti, Evangelia, additional, Basford, Christina, additional, Green, Anthony R., additional, Wilson, Nicola K., additional, Howe, Steven J., additional, Dick, John E., additional, Göttgens, Bertie, additional, Francis, Natalie, additional, and Laurenti, Elisa, additional

Published
2022
Full Text
View/download PDF

8. Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Author

Mende, Nicole; https://orcid.org/0000-0002-5078-2333, Bastos, Hugo P; https://orcid.org/0000-0002-8072-4070, Santoro, Antonella; https://orcid.org/0000-0002-1012-888X, Mahbubani, Krishnaa T; https://orcid.org/0000-0002-1327-2334, Ciaurro, Valerio; https://orcid.org/0000-0003-0150-1037, Calderbank, Emily Francesca, Quiroga Londoño, Mariana; https://orcid.org/0000-0003-2352-0773, Sham, Kendig, Mantica, Giovanna; https://orcid.org/0000-0002-7429-7236, Morishima, Tatsuya, Mitchell, Emily, Lidonnici, Maria Rosa, Meier-Abt, Fabienne; https://orcid.org/0000-0001-5337-6210, Hayler, Daniel, Jardine, Laura, Curd, Abbie, Haniffa, Muzlifah, Ferrari, Giuliana; https://orcid.org/0000-0003-0790-3133, Takizawa, Hitoshi; https://orcid.org/0000-0002-5276-5430, Wilson, Nicola K; https://orcid.org/0000-0003-0865-7333, Gottgens, Bertie, Saeb-Parsy, Kourosh; https://orcid.org/0000-0002-0633-3696, Frontini, Mattia; https://orcid.org/0000-0001-8074-6299, Laurenti, Elisa; https://orcid.org/0000-0002-9917-9092, Mende, Nicole; https://orcid.org/0000-0002-5078-2333, Bastos, Hugo P; https://orcid.org/0000-0002-8072-4070, Santoro, Antonella; https://orcid.org/0000-0002-1012-888X, Mahbubani, Krishnaa T; https://orcid.org/0000-0002-1327-2334, Ciaurro, Valerio; https://orcid.org/0000-0003-0150-1037, Calderbank, Emily Francesca, Quiroga Londoño, Mariana; https://orcid.org/0000-0003-2352-0773, Sham, Kendig, Mantica, Giovanna; https://orcid.org/0000-0002-7429-7236, Morishima, Tatsuya, Mitchell, Emily, Lidonnici, Maria Rosa, Meier-Abt, Fabienne; https://orcid.org/0000-0001-5337-6210, Hayler, Daniel, Jardine, Laura, Curd, Abbie, Haniffa, Muzlifah, Ferrari, Giuliana; https://orcid.org/0000-0003-0790-3133, Takizawa, Hitoshi; https://orcid.org/0000-0002-5276-5430, Wilson, Nicola K; https://orcid.org/0000-0003-0865-7333, Gottgens, Bertie, Saeb-Parsy, Kourosh; https://orcid.org/0000-0002-0633-3696, Frontini, Mattia; https://orcid.org/0000-0001-8074-6299, and Laurenti, Elisa; https://orcid.org/0000-0002-9917-9092

Abstract

Rare hematopoietic stem and progenitor cell (HSPC) pools outside the bone marrow (BM) contribute to blood production in stress and disease but remain ill-defined. Although non-mobilized peripheral blood (PB) is routinely sampled for clinical management, the diagnosis and monitoring potential of PB HSPCs remains untapped, as no healthy PB HSPC baseline has been reported. Here we comprehensively delineate human extramedullary HSPC compartments comparing spleen, PB and mobilized PB (mPB) to BM using single-cell RNA-seq and/or functional assays. We uncover HSPC features shared by extramedullary tissues and others unique to PB. First, in contrast to actively dividing BM HSPCs, we find no evidence of substantial ongoing hematopoiesis in extramedullary tissues at steady state, but report increased splenic HSPC proliferative output during stress erythropoiesis. Second, extramedullary stem cells/multipotent progenitors (HSC/MPPs) from spleen, PB and mPB share a common transcriptional signature and increased abundance of lineage-primed subsets compared to BM. Third, healthy PB HSPCs display a unique bias towards erythroid-megakaryocytic differentiation. At HSC/MPP level, this is functionally imparted by a subset of phenotypic CD71+ HSC/MPPs, exclusively producing erythrocytes and megakaryocytes, highly abundant in PB but rare in other adult tissues. Finally, the unique erythroid-megakaryocytic-skewing of PB is perturbed with age, in essential thrombocythemia and in beta-thalassemia. Collectively, we identify extramedullary lineage-primed HSPC reservoirs that are non-proliferative in situ and report involvement of splenic HSPCs during demand-adapted hematopoiesis. Our data also establish aberrant composition and function of circulating HSPCs as potential clinical indicators of BM dysfunction.

Published
2022

9. 3214 – DNMT3A R882 MUTATION IN HUMAN HAEMATOPOIETIC STEM CELLS ALTERS DIFFERENTIATION TOWARDS NEUTROPHILS AND MONOCYTES

Author

Vedi, Aditi, primary, Hayler, Daniel, additional, Biezuner, Tamir, additional, Mitchell, Emily, additional, Sham, Kendig, additional, Krzywon, Aleksandra, additional, Bastos, Hugo, additional, Lee, Joe, additional, Tuval, Amos, additional, Danin, Adi, additional, Chapal, Noa, additional, Santoro, Antonella, additional, Moskovitz, Yoni, additional, Minden, Mark, additional, Arruda, Andrea, additional, Nangalia, Jyoti, additional, Campbell, Peter, additional, Shlush, Liran, additional, and Laurenti, Elisa, additional

Published
2022
Full Text
View/download PDF

11. STAT1 is essential for HSC function and maintains MHCIIhistem cells that resist myeloablation and neoplastic expansion

Author

Li, Juan, Williams, Matthew J., Park, Hyun Jung, Bastos, Hugo P., Wang, Xiaonan, Prins, Daniel, Wilson, Nicola K., Johnson, Carys, Sham, Kendig, Wantoch, Michelle, Watcham, Sam, Kinston, Sarah J., Pask, Dean C., Hamilton, Tina L., Sneade, Rachel, Waller, Amie K., Ghevaert, Cedric, Vassiliou, George S., Laurenti, Elisa, Kent, David G., Göttgens, Berthold, and Green, Anthony R.

Abstract

•STAT1 is essential for normal HSC function and maintenance of a MHCIIhiHSC subset that is less responsive to stress-induced proliferation.•MHCIIhiand MHCIIlosubsets both contain functional HSCs, but MHCIIloHSCs show increased Mk potential and are expanded in mutant CALR mice.

Published
2022
Full Text
View/download PDF

12. Quantitative and molecular differences distinguish adult human medullary and extramedullary haematopoietic stem and progenitor cell landscapes

Author

Mende, Nicole, primary, Bastos, Hugo P, additional, Santoro, Antonella, additional, Sham, Kendig, additional, Mahbubani, Krishnaa T., additional, Curd, Abbie, additional, Takizawa, Hitoshi, additional, Wilson, Nicola K, additional, Göttgens, Bertie, additional, Saeb-Parsy, Kourosh, additional, and Laurenti, Elisa, additional

Published
2020
Full Text
View/download PDF

13. Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Author

Nicole Mende, Hugo P. Bastos, Antonella Santoro, Krishnaa T. Mahbubani, Valerio Ciaurro, Emily F. Calderbank, Mariana Quiroga Londoño, Kendig Sham, Giovanna Mantica, Tatsuya Morishima, Emily Mitchell, Maria Rosa Lidonnici, Fabienne Meier-Abt, Daniel Hayler, Laura Jardine, Abbie Curd, Muzlifah Haniffa, Giuliana Ferrari, Hitoshi Takizawa, Nicola K. Wilson, Berthold Göttgens, Kourosh Saeb-Parsy, Mattia Frontini, Elisa Laurenti, University of Zurich, Mende, Nicole [0000-0002-5078-2333], Bastos, Hugo P [0000-0002-8072-4070], Santoro, Antonella [0000-0002-1012-888X], Mahbubani, Krishnaa T [0000-0002-1327-2334], Ciaurro, Valerio [0000-0003-0150-1037], Quiroga Londoño, Mariana [0000-0003-2352-0773], Mantica, Giovanna [0000-0002-7429-7236], Meier-Abt, Fabienne [0000-0001-5337-6210], Ferrari, Giuliana [0000-0003-0790-3133], Takizawa, Hitoshi [0000-0002-5276-5430], Wilson, Nicola K [0000-0003-0865-7333], Saeb-Parsy, Kourosh [0000-0002-0633-3696], Frontini, Mattia [0000-0001-8074-6299], Laurenti, Elisa [0000-0002-9917-9092], Apollo - University of Cambridge Repository, Mende, Nicole, Bastos, Hugo P, Santoro, Antonella, Mahbubani, Krishnaa T, Ciaurro, Valerio, Calderbank, Emily F, Quiroga Londoño, Mariana, Sham, Kendig, Mantica, Giovanna, Morishima, Tatsuya, Mitchell, Emily, Lidonnici, Maria Rosa, Meier-Abt, Fabienne, Hayler, Daniel, Jardine, Laura, Curd, Abbie, Haniffa, Muzlifah, Ferrari, Giuliana, Takizawa, Hitoshi, Wilson, Nicola K, Göttgens, Berthold, Saeb-Parsy, Kourosh, Frontini, Mattia, and Laurenti, Elisa

Subjects
Adult, 10039 Institute of Medical Genetics, Stem Cells, Immunology, 610 Medicine & health, Bone Marrow Cells, Cell Biology, Hematology, Hematopoietic Stem Cells, Biochemistry, Hematopoiesis, Bone Marrow, 10032 Clinic for Oncology and Hematology, 570 Life sciences, biology, Humans, Erythropoiesis, Megakaryocytes
Abstract

Rare hematopoietic stem and progenitor cell (HSPC) pools outside the bone marrow (BM) contribute to blood production in stress and disease but remain ill-defined. Although nonmobilized peripheral blood (PB) is routinely sampled for clinical management, the diagnosis and monitoring potential of PB HSPCs remain untapped, as no healthy PB HSPC baseline has been reported. Here we comprehensively delineate human extramedullary HSPC compartments comparing spleen, PB, and mobilized PB to BM using single-cell RNA-sequencing and/or functional assays. We uncovered HSPC features shared by extramedullary tissues and others unique to PB. First, in contrast to actively dividing BM HSPCs, we found no evidence of substantial ongoing hematopoiesis in extramedullary tissues at steady state but report increased splenic HSPC proliferative output during stress erythropoiesis. Second, extramedullary hematopoietic stem cells/multipotent progenitors (HSCs/MPPs) from spleen, PB, and mobilized PB share a common transcriptional signature and increased abundance of lineage-primed subsets compared with BM. Third, healthy PB HSPCs display a unique bias toward erythroid-megakaryocytic differentiation. At the HSC/MPP level, this is functionally imparted by a subset of phenotypic CD71+ HSCs/MPPs, exclusively producing erythrocytes and megakaryocytes, highly abundant in PB but rare in other adult tissues. Finally, the unique erythroid-megakaryocytic–skewing of PB is perturbed with age in essential thrombocythemia and β-thalassemia. Collectively, we identify extramedullary lineage-primed HSPC reservoirs that are nonproliferative in situ and report involvement of splenic HSPCs during demand-adapted hematopoiesis. Our data also establish aberrant composition and function of circulating HSPCs as potential clinical indicators of BM dysfunction.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results