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Background: Chronic Obstructive Pulmonary Disease (COPD) is associated with autonomic nerve dysfunction. Nonlinear methods of heart rate variability (HRV) analysis have been gaining interest as a method of detecting changes in cardiac autonomic nerve function. Regular practice of physical exercise may cause improvement of this impaired autonomic nerve function. Objectives: To assess the effect of physical exercise on nonlinear measures of HRV analysis in male patients with COPD. Methods: This prospective interventional study was carried out in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka in 2018 on 60 diagnosed male COPD patients aged 40-75 years with disease duration of at least 5 years selected from the Department of Respiratory Medicine, BSMMU, Dhaka. Thirty patients underwent physical exercise (walking for 30 minute and pursed lip and diaphragmatic breathing 10 minuts)for 3 months in addition to standard treatment. Thirty (30) patients received only standard treatment for 3 months. For comparison, 30 age matched apparently healthy males were also enrolled. HRV data of all patients were recorded before and after 3 months of follow up. HRV data of healthy controls were recorded once at baseline. For assessing autonomic dysfunction, nonlinear parameters of HRV were recorded by Power Lab 8/35. For statistical analysis, paired sample t-test and independent sample t-test were done as applicable. Results: SD1, SD2 and SD1/ SD2 were found significantly lower in COPD patients compared to healthy controls at baseline. After 3 months of physical exercise, significant increment of these parameters occurred with a trend of improvement in cardiac autonomic nerve function in these patients. Conclusion: This study concluded that physical exercise can improve cardiac autonomic nerve dysfunction function in COPD patients. J Bangladesh Soc Physiol. 2022, June; 17(1): 6-13

Background: Metabolic syndrome (MetS) is a major risk factor for cardiac mortality and morbidity. Hyperhomocysteinemia and folic acid are related with MetS. Both MetS and hyperhomocysteinemia adversely affect heart rate variability (HRV). Objectives: To assess the relationship of vitamin B12, folic acid and homocysteine levels with HRV in female MetS patients. Methods: This cross sectional study was carried out on 40 female MetS patients of 25-45 years of age. Forty apparently healthy female with similar age were control. Chemiluminescent immunoassay measured serum vitamin B12, folic acid, homocysteine level and time domain parameters of HRV were recorded by Powerlab 8/35, AD instruments, Australia. Independent sample ‘t’ test and pearson correlation coefficient test was used for statistical analysis Results: In this study, all the time domain HRV variables ( Standard deviation of all RR intervals-SDRR, Coefficient variation of RR interval-CVRR, The square root of mean squared difference of RR intervals-RMSSD and Number of R-R interval differing >50ms from adjacent intervals divided by the total number of all R-R intervals-pRR50%) were significantly lower (p

Background: Breast milk is very much essential for proper growth, development and wellbeing of children. They should be breastfed within 1 hour of birth, exclusively breastfed for first six months of life. Breastfeeding difficulties are common. Maternal, infant and social factors are involved in establishment of proper breastfeeding. Objective: To find out the difficulties that a mother face during breastfeeding. Methodology: Cross-sectional observational study was carried out Shaheed Suhrawardy Medical Collage & Hospital (Sh.S.MCH). All children upto 6 months of age and their mother attending in OPD, Breast feeding corner and pediatrics indoor of Sh.S.MCH during study period. Results: Total 19 (38%) infants had difficulty in breast feeding related to maternal factors. Majority (63%) of mother complained of not enough milk, but urine output of infants was ≥6 times within 24 hours in 7 infants of these mothers. Three mothers had stopped breast feeding due to sickness. Total 25 (50%) infants had difficulty in breast feeding related to infant factors. Majority 17 (68%) of them were crying babies, but urine output was ≥6 times in 24 hours in 12 of these infants. Refusal of breast was found in 6 (24%) infants, sickness was cause for stopping breastfeeding found in 2 (8%) infants. Total 13 (26%) infants had difficulty in breast feeding related to social factors. Majority 8 (67%) were working mother and 5 (33%) mothers said that there was lack of familial support. Conclusion: Most of the mothers initiated breast feeding 1 hour after birth. Most of the mothers had given honey as prelacteal feeding to their infants. Most of them were on partial breast feeding. Sixty five percent infants were given formula milk. Difficulties in breast feeding were related to infant factors and maternal factors mostly. More than one factor was present in some infant. EMCJ. January 2022; 7(1): 11-14

The experiment was conducted to assess and compare the effect of micronutrient enrich commercial inorganic fertilizer and macronutrient enrich traditional inorganic fertilizer on plankton abundance in the manmade earthen ponds of Khulna University pond complex II for a period of three months from August to October 2017. There were three treatments each with three replications. Macro fertilizers (Urea 2.45 g m-1 week-1, TSP 2.45 g m-1 week-1 and molasses–yeast mixture 3.09 g m-1 week-1) were applied in treatment-1 (T1) while commercial inorganic fertilizer (4.5 g m-1 week-1) was applied in treatment-2 (T2) while no fertilizer was applied in treatment-3 (T3). Four different classes of phytoplankton namely Chlorophyceae, Cyanophyceae, Bacillariophyceae, and Euglenophycaea were found in this experiment in which Chlorophyceae (5304.25±223.78 cells L-1) was the dominant class in respect of abundance and number of genera. Additionally, four classes of zooplankton such as Copepoda, Cladocera, Rotifera, Ostracoda were observed in which Copepoda (1559.84±70.48 cells L-1) was the most dominant class in treatment T2 than other treatments. Mean abundance of different phytoplanktons were 8864.77±52.78, 11489.04±295.76, 5983.04±272.88 (cells L-1) in T1, T2 and T3 and mean abundance of zooplankton under treatments T1, T2 and T3 were 3997.65±126.78, 4621.64±45.67, 3017.66 ±137.67 (cells L-1) respectively. The results indicated that mean abundance of phytoplankton and zooplankton were significantly higher (P

The present study deciphers the extent of the activity of heterospecific mixing of gametes and production of hybrids that are available as food fishes in the markets of Khulna, Jessore and Satkhira districts during March to July, 2009. The taxonomic characters of two putative hybrids from 150 samples namely Catla catla × Labeo rohita and Labeo rohita × Labeo calbasu were studied and compared with their original species and significant differences were found in most of the meristic characters and morphometric proportionsat 5% level of significance. The two hybrids were not found to be intermediate between their original species for most of the characters. Catla catla × Labeo rohita deflected more toward catla; while Labeo rohita × Labeo calbasu toward calbasu, however, the hybrid does not have barbel as with calbasu. The results of the study revealed a grave situation of fishes’ gene pool mixing in the hatcheries.

Carotid cavernous fistulas (CCF) are abnormal communications inducing shunting of blood between the carotid arterial system and the cavernous sinus (CS), resulting in flow reversal in the veins draining through the CS.1 CCFs can be classified as spontaneous or traumatic according to the etiology and direct or indirect (dural) according to the anatomical features2. The authors have dealt a case of a spontaneous indirect Carotid-Cavernous Fistula in a young male of 30 on the right side. The patient presented with headache and bulging of right eye. On examination there was pulsatile proptosis on the right side with conjunctival congestion. CT angiography revealed CCF on the right side with dilated, tortuous right ophthalmic and common facial vein. DSA revealed indirect Carotid-Cavernous Fistula fed by right middle meningeal artery (MMA), accessory meningeal artery and branch of right ophthalmic artery(OA) drained by right ophthalmic vein with abnormal communication via right common facial vein. Patient underwent transarterial embolization with ONYXR . DSA revealed total obliteration of fistula. Headache subsided and proptosis reduced immediately after embolization on procedure table with intact neurology. Almost two months down the follow up revealed complete recovery of proptosis and conjunctival congestion with no further headache. Bang. J Neurosurgery 2022; 11(2): 128-133

Background: Cervical cancer-a preventable disease, is the second most commonly diagnosed cancer among women of developing countries. Paps smear is the gold standard screening method worldwide. But a low-cost test, visual inspection of cervix with acetic acid (VIA) has been emerged as an alternative for use in lowresource settings. VIA is simple and easy to perform by auxiliary health professionals. Colposcopy is an essential procedure in the evaluation of screen positive cervix though it requires considerable training and experience. Objective: The objective of this study was to evaluate the VIA positive cases by colposcopy, colposcopy guided biopsy and histopathological examination. Methods: This was a cross sectional observational study conducted among 100 VIA positive cases attended at GOPD of Dhaka Medical College Hospital from July 2014 to December 2014. Colposcopy was performed in all VIA positive cases. Subsequent biopsy and histopathological examinations were done for those with colposcopic abnormal findings. Results: The mean age of the study population was 37.9±9.3 years. Among VIA positive cases 66.0% had complaints of whitish vaginal discharge. Among the study population, colposcopically 46.0% had healthy cervix (normal) and 54.0% had abnormal findings. Colposcopy guided biopsy was taken from those with abnormal findings. Histopathological findings were normal in 38.9% cases, chronic cervicitis in 20.37% cases, squamous cell metaplasia in 3.7% cases, CIN I in 16.7%, CIN II in11.11%, CIN III in 5.6% and 3.7% cases were carcinoma cervix. True positive value was 20, false positive value 34 and positive predictive value of colposcopy was 37.03%. Conclusion: This study concluded that VIA is effective in detection of CIN and invasive carcinoma of cervix. It also suggests the role of colposcopy in the evaluation of CIN and other cervical diseases in screen positive cases. VIA can be used as a screening tool in low and middle income countries like Bangladesh, not only in rural areas and small health centres, but also in hospitals, cancer institutes, and other health facilities with better resources. Bangladesh Med Res Counc Bull 2022; 48(1): 27-32

Background: Vulvar cancer is one of the rare malignancies of the female genital tract. Vulvar cancer is predominantly a disease of post-menopausal multiparous women. Objective: The aim of this study was to analyse and report the clinico-pathological features and treatment modalities of patients with vulvar cancer treated at National Institute of Cancer Research and Hospital (NICR&H), Dhaka, Bangladesh. Methods: This was a cross- sectional observational study conducted among 96 vulvar cancer cases treated at National Institute of Cancer Research and Hospital (NICR&H), Dhaka, Bangladesh from 2015 to 2020. The medical records of patients with vulvar cancer were retrospectively reviewed after obtaining approval from the Institutional Review Board (IRB). Clinical details of all vulvar cancer patients and treatment given were collected from the hospital records and were reviewed retrospectively. Results: A total of 96 patients with vulvar cancer were included for this retrospective analysis. The median age was 50 years (range, 12- 85 years) with a mean (±SD) of 50.50±15.12 years. Stages of the disease were: stage I: 27.1% patients; stage II: 22.9% patients; stage III: 30.3% patients; stage IV: 16.7% patients; and unknown stage: 3.1% patient as per International Federation of Gynecology and Obstetrics (FIGO) staging system. Histopathologically, squamous cell carcinoma (SCC) was the most common histologic type (84.4%) followed by adenocarcinoma (5.2%), melanoma (4.2%), basal cell carcinoma (3.1%), and others (3.1%). Most of the patients (45.8%) were treated by surgery with or without adjuvant radiotherapy. About 24.0% of patients were treated primarily by radiotherapy and/or chemo-radiotherapy. Conclusion: Management of vulvar cancer requires multidisciplinary team approach, which is available only in tertiary care centres. The treatment of vulvar cancer is surgery, radiotherapy, chemo- radiotherapy or a combination therapy. Treatment should be individualised based on lesions location and histological type. Bangladesh Med Res Counc Bull 2022; 48(1): 64-70

Background & objective: Pregnant women with a previous caesarian section may be offered either vaginal birth or elective repeat caesarian section (ERCS). Detailed antenatal counseling and methodical intrapartum management of women are the key factors for success of planned vaginal birth after caesarean (VBAC). The present study was undertaken to determine the incidence of successful vaginal birth and the maternal and perinatal complications in patients undergoing planned vaginal birth after one previous caesarean section. Methods: This prospective observational study was carried out in the Department of Obstetrics and Gynaecology, Sir Salimullah Medical College & Mitford Hospital (SSMC & MH), Dhaka over a period of one year between January to December 2016. A total of 96 term pregnant women, aged 20 – 35 years, having previous experience of one lower uterine caesarean section with spontaneous onset of labour, vertex presentation of the fetus and adequate pelvis were consecutively included in the study. The main primary outcome variable was fate of trial VBAC (successful vaginal delivery either spontaneous or assisted or ERCS). The secondary outcome variables were maternal and perinatal complications (morbidity and mortality). Result: Over 55% of the women under trial VBAC were 25 – 30 years old with mean age of the women being 26.8 ± 3.7 years. Nearly three-fifths (58.3%) of the women were of normal BMI, 40.6% were overweight. None of the women was obese. Only 15% women had gestational age 40 weeks. The mean interval between the current and the previous births was almost 3 years. In terms of primary outcome, over 60% of the planed VBAC were successfully delivered (52.2% spontaneously and 9.3% with the aid of forceps and vacuum extraction). While maternal complications were uterine rupture (6.2%), hysterectomy (6.2%) and haemorrhage needing transfusion (14.6%), neonatal complications were respiratory distress syndrome (17.7%), perinatal death (10.4%), hypoxic ischemic encephalopathy (4.2%) and sepsis (8.3%). Conclusion: The study concluded that planned VBAC is appropriate for majority of women with a singleton pregnancy of cephalic presentation who have had a single previous lower segment caesarean section (LSCS). However, as the maternal complications (like hysterectomy, haemorrhage etc.) and the perinatal complications including death were much high, utmost caution is advised in selecting the right candidate for VBAC so that the incidence of successful VB could be maximized and complications minimized. Ibrahim Card Med J 2021; 11 (1): 54-61

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease affecting multiple small symmetrical joints with extra-articular involvement. Altered blood mineral status is associated with the immune-inflammatory rheumatoid process. Reduced heart rate variability (HRV) is a predictor of sudden cardiac death in RA patients. So, the role of minerals for altered HRV needs investigation in RA. Objectives: To assess the relationship between HRV and serum zinc (Zn), magnesium and (Mg) levels in RA patients. Methods: This cross sectional study enrolled 60 female participants of 30-50 years of age. Among them 30 female RA patients were included in the study group and 30 age matched apparently healthy women constituted control group. Serum Zn, Mg levels were estimated by autoanalyzer and frequency domain parameters of HRV were recorded by a data acquisition device, Powerlab 8/35, AD Instruments, Australia followed by analysis with software, Lab chart. Statistical analyses were done by independent sample ‘t’ test and Pearson’s correlation coefficient test. Results: In this study, among frequency domain parameters of HRV, total power (TP), low frequency power(LF), high frequency power(HF), HF normalized unit(HF nu) were significantly lower (p

Background: Polycystic ovary syndrome (PCOS) is a well recognized reproductive hormone disorder. It is often associated with dysautonomia and increased oxidative stress Objective: To assess the relationship between cardiac autonomic nerve function (CANF) and oxidative stress in patients with PCOS. Methods: This cross sectional study enrolled 30 newly diagnosed PCOS patients 20-35 years of age and 30 apparently healthy, regularly menstruating women of similar age and BMI. The patients were enrolled from outpatient Department of Endocrinology, BSMMU, Dhaka. CANF was assessed by poincare analysis of RR interval, a non linear method of Heart Rate Variability (HRV) analysis. HRV data of all subjects were recorded by a digital data acquisition device, Power lab 8/35, AD instruments, Australia, with lab chart software analysis. Oxidative stress was assessed by plasma catalase and plasma Malondialdehyde (MDA) levels. For statistical analysis unpaired “t” test and Pearson’s correlation test were used. Results: In this study, resting pulse rate, systolic blood pressure(SBP), diastolic blood pressure(DBP) were significantly higher (p0.05) in PCOS patients. In addition SD1/SD2 ratio showed positive correlation with plasma catalase and negative correlation with plasma MDA levels (p>0.05) in these group of patients. But all these correlations were statistically not significant. Conclusion: The present study reveals association of autonomic dysfunction and higher oxidative stress with PCOS but it failed to show evidence for any interrelationship between them. J Bngladesh Soc Physiol 2021;16(1): 88-94

Background: Polycystic ovary syndrome (PCOS) is a very common reproductive endocrine disorder. Altered cardiovascular autonomic modulation and oxidative stress may predispose PCOS patients to cardiac events in the long term. Objective: To assess the relationship between sympathetic autonomic reactivity and oxidative stress in patients with polycystic ovary syndrome. Methods: This cross-sectional study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka from September 2018 to August 2019 on 35 newly diagnosed obese (BMI ≥25kg/m2) PCOS patients aged 20-35 years. Age and BMI matched 35 apparently healthy subjects were also enrolled. Two noninvasive conventional autonomic function tests, such as systolic blood pressure (SBP) fall during active standing and diastolic blood pressure (DBP) rise during sustained handgrip were conducted on all subjects for evaluation of sympathetic reactivity. For assessment of oxidative stress, plasma malondialdehyde level as oxidant and plasma catalase level as antioxidant were measured in all subjects by spectrophotometry. For statistical analysis Independent sample “t” test, Pearson’s correlation test and multiple regression analysis were done. Results: In this study PCOS patients had significantly higher resting heart rate, SBP and DBP than healthy control (p

Background: Metabolic syndrome (MeS) is a well-recognized risk factor for cardiovascular diseases. It is associated with hyperhomocysteinemia resulting from deficiency of vitamin B12 and folic acid. Both the MeS and hyperhomocysteinemia adversely affect heart rate variability. Objectives: To assess vitamin B12, folic acid and homocysteine levels in female metabolic syndrome patients and their relationship with heart rate variability. Methods: After taking ethical clearance from Institutional Review Board (IRB) of Bangabandhu Sheikh Mujib Medical University (BSMMU) this cross sectional study was conducted on 80 female subjects of 25-45 years of age. Among them 40 metabolic syndrome patients were included in the study group and 40 age matched apparently healthy female constituted control group. All the patients were enrolled from Outpatient Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh according to selection criteria. Serum vitamin B12, folic acid, homocysteine level was estimated by chemiluminescent immunoassay and frequency domain parameters of Heart Rate Variability (HRV) was recorded by a data acquisition device, Powerlab 8/35, AD instruments, Australia. Statistical aalysis was done by independent sample ‘t’ test and pearson correlation test. Results: In this study, among frequency domain HRV variables Total power(TP), low frequency power(LF), high frequency power (HF), HF normalized unit(nu) were significantly lower (p0.05) were found in serum vitamin B12, folic acid and homocysteine levels between these two groups. But on correlation analysis only the folic acid level was significantly negatively correlated (p

Aim: Landmark-based truss network system was applied to examine the morphometric and meristic differences among four different populaces of Macrognathus aculeatus namely; Dhakuriabeel, Jashore (DBJ); Arial Kha River, Madaripur (AKRM); Nabaganga River, Jhenaidah (NRJ); Kopotakkha River, Jashore (KRJ) in Bangladesh. Methodology: Completely 80 samples of M. aculeatus were collected (20 individuals from each population) for this study. Six meristic characters, 6 morphometric characters and 15 truss-based morphometric characters were measured for one-way analysis of variance (ANOVA) and Tukey-HSD post hoc test. Discriminant function analyses were also performed by using morphometric and truss-based morphometric characters to detect potential characters responsible for population separation. Results: Completely 80 samples of M. aculeatus were collected (20 individuals from each population) for this study. Six meristic characters, 6 morphometric characters and 15 truss-based morphometric characters were measured for one-way analysis of variance (ANOVA) and Tukey-HSD post hoc test. Discriminant function analyses were also performed by using morphometric and truss-based morphometric characters to detect potential characters responsible for population separation. Interpretation: The baseline information derived from the present study would be useful for appropriate conservation of M. aculeatus populations as well as for the researchers for further investigation.

HPV infection is estimated to be responsible for about 5% of human cancers worldwide. Among all HPV-associated malignancies, cervical cancer is the most important cause of morbidity and mortality worldwide. Cervical cancer is the fourth most common cancer in women worldwide and leading cause of cancer death among females in less developed countries. Persistent HPV infection is a necessary cause of invasive cervical cancer with a prevalence of 99.7% in cervical cancer worldwide.At least 70% of cervical cancers are caused by HPV 16 and HPV 18. HPV vaccination in combination with regular screening offers the most effective way for women to be protected against cervical cancer.The global burden of cervical cancer falls heaviest on the developing countries which haven’t introduced the HPV vaccine as part of their national public health strategy to prevent and control cervical cancer. Different studies showed a drop in the prevalence of HPV associated diseases in vaccinated populations. Sustained efficacy, immunogenicity, and safety of the bHPV vaccine were observed in the final analysis of a follow-up study up to 9.4 years postvaccination. Cervical cancer is a major public health problem in Bangladesh. There has been little success with screening program against cervical cancer in Bangladesh. Therefore, a National HPV Vaccination Program seems to be the window of opportunity to reduce the mortality and morbidity of cervical cancer in Bangladesh. J Bangladesh Coll Phys Surg 2021; 39(2): 123-131

The aim of this study was to determine whether histone deacetylase inhibitors (HDACi), including entinostat (MS-275), valproic acid (VPA), trichostatin A (TSA), and sodium butyrate (NaB), promoted the odontogenic differentiation of the odontoblast-like cell line, MDPC-23 in the absence of an osteoblast mineralization medium. The cells were cultured in basal medium (Dulbecco's modified Eagle medium) with and without (controls) the inhibitors. The cell viability and migration were assessed using the cell proliferation reagent WST-1 and a scratch wound healing assay, respectively. The mRNA expression levels of bone morphogenetic protein (Bmp)-2 and -4, collagen 1 alpha 1 (Col1α1), osteocalcin (Oc), dentin matrix protein 1 (Dmp1), dentin sialophosphoprotein (Dspp), runt-related transcription factor 2 (Runx2), Krueppel-like factor 5 (Klf5), and Msh homeobox 1 (Msx1) were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Alizarin red and alkaline phosphatase assays were performed to determine the extent of mineralization in the culture systems. No significant differences in cell numbers were observed between the controls and the MS-275-, VPA-, and NaB-treated cells; however, a significant difference was observed with TSA (concentration, 1000 nM). The scratch wound healing assay showed no effect of cell migration in the MS-275 (1.0 µM)-treated cells when compared with the controls at 24 h. Furthermore, MS-275, VPA, and NaB increased the mRNA expression levels of Bmp-2 and -4, Oc, and Runx2 followed by the mineralization of the cells. Only MS-275 significantly increased the expression levels of Dmp1, Dspp, Klf5, and Msx1 in the cells. These findings indicated that MS-275 may be considered as a reliable candidate for the odontogenic differentiation of dental pulp cells.

Background:Vitamin D3 deficiency is common in Asthma COPD overlap syndrome (ACO) and administration of vitamin D3 increased heart rate variability in healthy subjects. Objective: This randomized clinical trial aimed to investigate the therapeutic effect of vitamin D3administration on time domain measures of heart rate variability in 51 male vitamin D3(

Background: Diabetes mellitus (DM) is a disorder with a debilitating effects on cardiac autonomic control. Multiple major cardiovascular risk factors associated with DM led diabetic patients at high risk of Cardiovascular Disease. Objective: To assess cardiovascular responses to tilting in Type 2 Diabetic patients (T2DM) with normal and abnormal autonomic function test. Methods: This experimental study was conducted on 60 patients of T2DM. Among them, 30 patients were with normal cardiovascular reflex test (group DN) and 30 patients were with abnormal test (group DA). Thirty(30) apparently healthy subjects with similar age and sex without any physical illness were enrolled as control. Tilt table test of all subjects was done by tilting at 60° for 10 min by using a motorized tilt table. Cardiovascular response to tilt test was assessed by calculating D Heart rate (Acceleration index and Brake index); DSBP (SBP 30s-0 and SBP1 min -0), DDBP (DBP 30s-0 and DBP1 min -0) after tilting. For statistical analysis, one-way ANOVA followed by Bonferroni post hoc was used. Results: In this study, the Acceleration index was significantly higher in patient group DN compared to control and DA(p

The gut microbiome has an important role in infant health and development. We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life. A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates. The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology. We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp. infantis, indolelactate and N-acetylglutamate. Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.

Background: Motor bike is a popular two wheeler vehicle in Bangladesh especially among young generation, newly married couple and service holders. It is also popular vehicle among female NGO workers. Female like to travel on motor bike along with life partner or with guardian hanging their both legs on one side of seat increasing the tendency to fall down from motor cycle due to imbalance body weight during turning the road or crossing the uneven road or sudden increasing the speed of the motor bike or crossing on a speed breaker. Aims: Encourage female to travel on motor bike hanging their legs on both sides of the seat to maintain the body weight balance for reducing the mortality and morbidity of RTA. Methods: All the female patients admitted in neurosurgery ward having motor cycle accident from January/2019- December/2019 were included in this study. Results: There is a strong association between fall from motor cycle (RTA) and hanging two legs on one side in female motor cycle traveler. Conclusions: female should travel on motor cycle hanging their legs on both side of seat to avoid avoidable head injury due to RTA. Bang. J Neurosurgery 2020; 10(1): 33-38

Background: Poincare plot analysis of heart rate variability (HRV) is a complex nonlinear method for assessing cardiac autonomic nerve function (CANF) in generalized anxiety disorder (GAD). Objective: This study aimed to observe the effect of music therapy on HRV by poincaré plot analysis in GAD patients. Methods: This prospective Interventional study was done in 2019 on 60 newly diagnosed GAD patients aged 20- 40 years, both male and female. Among them, 30 patients were under music therapy with Raga Bhairabi based Rabindra Sangeet for 3 months and 30 patients were without music therapy and both patient groups were studied over three months. Healthy controls, who were enrolled in this study, were Age, sex and BMI matched. Poincaré plot (non linear measure) of HRV of all GAD patients and control were recorded at baseline and after three months of follow up. HRV were recorded by Power Lab 8/ 35. For statistical analysis Bonferroni’s Post Hoc test and paired sample ‘t’ test were done. Results:Standard deviation of the points perpendicular to the axis of line of identity(SD1), standard deviation of the points along the axis of line of identity (SD2) SD and SD1/SD2 were significantly lower in all GAD patients compared to healthy control at baseline. After 3 months of music therapy, significant increment of these parameters occurred in GAD patients, whereas no changes of these except SD1 were noted in patients without music therapy after 3 months. Moreover, these parameters were not significantly different in patients under music therapy compared to control at the end of three months. Conclusion: This study concluded that music therapy can improve cardiac autonomic dysfunction in GAD patients. J Bangladesh Soc Physiol. 2020, June; 15(1): 39-45

Background: Tilt table test is used for the last few decades to detect cause in unexplained syncope. The response to tilting may vary physiologically with obesity. Objective: To assess the relationship of BMI to cardiovascular response to tilting. Methods: This experimental study was conducted from March 2019 to Feb 2020 on 90 healthy subjects with different BMI. Fifty one subjects of both gender with BMI 18.5-24.9 kg/m2 were included in the non-obese group and 39 subjects of both gender with BMI of 25-29.9 kg/m2 were included in overweight group and they were further subdivided into male and female. Head up tilting was done for 10 minutes at 600 by using a motorized tilt table. Systolic blood pressure (SBP), diastolic blood pressure (DBP) were recorded by an automatic sphygmomanometer. Heart rate (HR) and peripheral capillary oxygen saturation (SpO2) were measured by a pulse oximeter. For statistical analysis, Independent sample ‘t’ test, Pearson’s correlation test and Chi square tests were applied. Results: Significantly smaller rise of heart rate was observed in overweight males and greater fall of systolic blood pressure was observed in overweight females. Conclusion: This study concluded that over weight is associated with reduced orthostatic tolerance to head up tilt test in both genders. J Bangladesh Soc Physiol. 2020, June; 15(1): 6-10

The research was aimed to determine the present status of probiotics (Lactobacillus spp.) and pathogenic bacteria (Vibrio spp.) of culture pond as well as to determine whether the isolated probiotic Lactobacillus spp. can act as a controlling agent on experimentally pathogenic Vibrio spp. infected Penaeus monodon. In In-vitro test of gills and intestinal tracts, the potential antagonistic activity of Lactobacillus spp. toward Vibrio spp. was gradually observed at 4th hour of probiotic treatment. At 12th hour the viable count of Vibrio spp. was drastically reduced in gill from 2.33×105 to 1.03×103 (CFUg-1) and in intestinal tract 2.35×105 to 6.43×102 (CFUg-1). While, in In-vivo test, in case of muscle, gills and intestinal tract antagonistic activity of Lactobacillus spp. toward Vibrio spp. was noticed after 9 hours, 21 hours and 27 hours respectably of probiotic injected shrimps. However, only the result of in-vitro challenge test revealed that, Lactobacillus spp. significantly reduced the Vibrio spp. viable count of the gills of the selected samples (P=0.037). The investigation showed antagonistic effect of probiotic (Lactobacillus spp.) on experimentally Vibrio spp. infected shrimp. Asian J. Med. Biol. Res. June 2020, 6(2): 311-315