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Astaxanthin (AST) is a xanthophyll carotenoid with strong oxidation resistance, which can effectively scavenge various free radicals and protect organisms from oxidative damage. AST is also known to have prominent anti‐aging effects, but the underlying mechanism of AST in anti‐radiation aging is largely unknown. In this work, we applied ultraviolet (UV) irradiation to accelerate the aging of Caenorhabditis elegans (C. elegans) and treated the nematodes with AST to explore whether and how AST could attenuate the radiation‐induced aging effect. Our results showed that AST improved the survival rate of C. elegans, reduced the aging biomarkers, and alleviated the mitochondrial dysfunction caused by the irradiation. Based on the transcriptome sequencing analysis, we identified that the key genes regulated by AST were involved in JNK‐MAPK and DAF‐16 longevity signaling pathways. Furthermore, we employed jnk‐1 and daf‐16 mutants and verified the role of the JNK‐1/DAF‐16 signaling pathway in the anti‐aging effect. As such, this study has not only demonstrated that AST can resist the aging process caused by UV‐irradiation but also revealed the anti‐aging mechanism of AST through JNK‐1/DAF‐16 activation in C. elegans. [ABSTRACT FROM AUTHOR]

In order to determine Cd isotopic composition accurately, the key is to guarantee Cd recovery and reduce Sn interference.The recoveries of Cd with various separation and purification methods reported by previous studies show a large range variation from 42.6% to 99.8%, and the removal efficiency of Sn interference are various(87.8%-97.4%).Both the Cd recovery and Sn removal are not ideal.Reported in this study, 0.1 mol/L HBr-0.5 mol/L HNO3 mixture was determined to be a more effective solution to separate Cd from Sn.By increasing the amount of this mixture during leaching, more Sn can be removed from the samples without Cd loss.When 30 mL of mixture is used during leaching, more than 99.8% Sn can be leached out from the sample and the Cd recovery is 99.0%±0.5%.This method can be utilized for Cd isotope analyses of soil samples, offering new insight for high-precision Cd isotope measurement and serving as a technical method for Cd pollution tracing in the study of environmental soil.

Highlights: [•] The absorption spectra of [NiF6]4− clusters are theoretically calculated and assigned. [•] The calculation is based on complete diagonalization (of energy matrix) method. [•] The EPR spectra of [NiF6]4− clusters are studied by use of high-order perturbation formulas. [•] The local structure of [NiF6]4− clusters with rhombic symmetry (D 2h) are determined. [Copyright &y& Elsevier]