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8 results on '"Shao, Jia‐Le"'

4. Retropharyngeal Reduction Plate for Atlantoaxial Dislocation: A Cadaveric Test and Morphometric Trajectory Analysis

Author

Li, Jian‐yi, primary, Du, Yu‐kun, additional, Meng, Zhao, additional, Zhao, Zheng, additional, Hu, Hui‐qiang, additional, Shao, Jia‐le, additional, Tang, Xiao‐jie, additional, Kong, Wei‐qing, additional, Xu, Tong‐shuai, additional, Shao, Cheng, additional, Zhang, Yi‐xin, additional, and Xi, Yong‐ming, additional

Published
2022
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6. Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S

Author

LEI, Bin, SHAO, Jia-le, ZHANG, Feng, WANG, Jian, XIAO, Yun-hua, CHENG, Zhi-jun, TANG, Wen-bang, and WAN, Jian-min

Abstract

Development and application of the small-grain rice sterile line Zhuo201S (Z201S) has demonstrated its potential for mechanized hybrid rice seed production, leading to significant cost reductions. However, the molecular mechanism responsible for the small-grain size characteristic of Z201S remains unclear. In this study, we conducted a genetic analysis using near-isogenic lines constructed from Z210S, a small-grain rice sterile line, and R2115, a normal-grain variety. The results revealed that the small-grain trait in Z201S is governed by a single partially dominant gene, which also enhances grain number. Through mapping, we localized the causal gene to the short arm of chromosome 2, within a 113-kb physical region delimited by the molecular markers S2-4-1 and LB63. Transgenic analysis and gene expression assays highlighted LOC_Os02g14760as the most likely candidate gene, suggesting that the small-grain size trait of Z201S is controlled by a novel locus that has not been previously identified.

Published
2024
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7. Sinusoidal electromagnetic fields increase peak bone mass in rats by activating Wnt10b/β-Catenin in primary cilia of osteoblasts

Author

Hui-Ping Ma, Jian Zhou, Ke-Ming Chen, Cory J. Xian, Yu-Hai Gao, Bao-Ying Zhu, Jia-le Shao, Zhou, Jian, Gao, Yu-Hai, Zhu, Bao-Ying, Shao, Jia-Le, Ma, Hui-Ping, Xian, Cory J, and Chen, Ke-Ming

Subjects
0301 basic medicine, Peak bone mass, Small interfering RNA, Endocrinology, Diabetes and Metabolism, 030209 endocrinology & metabolism, Bone and Bones, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Calcification, Physiologic, Electromagnetic Fields, RNA interference, Osteogenesis, Animals, Orthopedics and Sports Medicine, Cilia, Femur, Cells, Cultured, beta Catenin, Gene knockdown, Osteoblasts, Chemistry, Cilium, Wnt signaling pathway, osteoblasts, Cell Differentiation, Organ Size, Wnt10b/β‐catenin, In vitro, Cell biology, Wnt Proteins, 030104 developmental biology, Animals, Newborn, Gene Expression Regulation, Catenin, Female, sinusoidal electromagnetic fields, Signal Transduction, primary cilium, Wnt10b
Abstract

Extremely low-frequency electromagnetic fields have been considered a potential candidate for the prevention and treatment of osteoporosis; however, their action mechanism and optimal magnetic flux density (intensity) parameter are still elusive. The present study found that 50-Hz sinusoidal electromagnetic fields (SEMFs) at 1.8 mT increased the peak bone mass of young rats by increasing bone formation. Gene array expression studies with femoral bone samples showed that SEMFs increased the expression levels of collagen-1α1 and Wnt10b, a critical ligand of the osteogenic Wnt/β-catenin pathway. Consistently, SEMFs promoted osteogenic differentiation and maturation of rat calvarial osteoblasts (ROBs) in vitro through activating the Wnt10b/β-catenin pathway. This osteogenesis-promoting effect of SEMFs via Wnt10b/β-catenin signaling was found to depend on the functional integrity of primary cilia in osteoblasts. When the primary cilia were abrogated by small interfering RNA (siRNA) targeting IFT88, the ability of SEMFs to promote the osteogenic differentiation of ROBs through activating Wnt10b/β-catenin signaling was blocked. Although the knockdown of Wnt10b expression with RNA interference had no effect on primary cilia, it significantly suppressed the promoting effect of SEMFs on osteoblastic differentiation/maturation. Wnt10b was normally localized at the bases of primary cilia, but it disappeared (or was released) from the cilia upon SEMF treatment. Interestingly, primary cilia were elongated to different degrees by different intensities of 50-Hz SEMFs, with the window effect observed at 1.8 mT, and the expression level of Wnt10b increased in accord with the lengths of primary cilia. These results indicate that 50-Hz 1.8-mT SEMFs increase the peak bone mass of growing rats by promoting osteogenic differentiation/maturation of osteoblasts, which is mediated, at least in part, by Wnt10b at the primary cilia and the subsequent activation of Wnt/β-catenin signaling. © 2019 American Society for Bone and Mineral Research.

Published
2019

8. [Preventive effect and mechanism of puerarin on rat models of disuse osteoporosis].

Author

Li K, Qin R, Shao JL, Gao YH, Zhou J, and Chen KM

Subjects
Animals, Female, Osteocalcin metabolism, Rats, Rats, Wistar, Tartrate-Resistant Acid Phosphatase metabolism, Bone Density, Isoflavones pharmacology, Osteoporosis drug therapy
Abstract

To investigate the preventive effect and possible mechanism of puerarin(Pur) in rat model of disuse osteoporosis(DOP),thirty healthy Wistar female rats of 2 months old were randomly divided into control group(Control), hindlimb suspension group(HLS), and puerarin group(HLS+Pur) in hindlimb suspension, with 10 rats in each group. A disuse osteoporosis model was established by tail suspension method, and 15.4 mg·kg~(-1) puerarin suspension was administered to HLS+Pur group every day, and the same volume of distilled water was administered to Control group and HLS group respectively. After 28 days, the rats were sacrificed by abdominal aorta blood collection, the main organs of the rats were removed, and the bone tissues of the rats were dissected. The organ index of the rats was calculated and the histopathology of the organs was observed under microscope. Bone mineral density test and bone biomechanical experiment were performed. Bone histomorphometry results were observed after bone tissue sectioning, and serum biochemical markers of bone metabolism were determined. There was no significant difference in organ index between the groups. There was no obvious abnormality in the pathological examination of the organs. The results of bone mineral density showed that puerarin could significantly increase the bone density of the tibia and vertebrae caused by hindlimb suspension. The mechanical parameters experiments showed that puerarin could effectively increase the maximum load and elastic modulus of the tibia and vertebrae. Fluorescence labeling showed that the fluorosis interval increased and the bone formation increased during puerarin treatment. The VG staining results showed that compared with the HLS group, in the puerarin group, the number of trabecular bone increased, the thickness of the trabecular bone became thicker, and the bone separation became smaller, which greatly improved the bone microstructure after hindlinb suspension. In addition, serum biochemical indicators showed that puerarin could promote bone formation index bone calcium. The content of osteocalcin(OC) increased and inhibited the formation of tartrate-resistant acid phosphatase 5 b(TRACP 5 b). Puerarin has a preventive effect in the rat model of disuse osteoporosis and its effect is good, and its mechanism may be related to promoting bone formation and inhibiting bone resorption.

Published
2019
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