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191 results on '"Shao, Jimin"'

6. Abstract B024: The thioredoxin system determines CHK1 inhibitor sensitivity via redox-mediated regulation of ribonucleotide reductase activity

Author

Prasad, Chandra Bhushan, primary, Oo, Adrian, additional, Qiu, Zhaojun, additional, Li, Na, additional, Singh, Deepika, additional, Xin, Xiwen, additional, Cho, Young-Jae, additional, Li, Zaibo, additional, Zhang, Xiaoli, additional, Yan, Chunhong, additional, Zheng, Qingfei, additional, Shao, Jimin, additional, Wang, Qi-En, additional, Kim, Baek, additional, and Zhang, Junran, additional

Published
2024
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12. The thioredoxin system determines CHK1 inhibitor sensitivity via redox-mediated regulation of ribonucleotide reductase activity

Author

Zhang, Junran, primary, Prasad, Chandra, additional, Oo, Adrian, additional, Qiu, Zhaojun, additional, Li, Na, additional, Singh, Deepika, additional, Xin, Xiwen, additional, Cho, Young-Jae, additional, Li, Zaibo, additional, Zhang, Xiaoli, additional, Yan, Chunhong, additional, Zheng, Qingfei, additional, Shao, Jimin, additional, Wang, Qi-En, additional, and Kim, Baek, additional

Published
2023
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15. Identification of Monobenzone as a Novel Potential Anti-Acute Myeloid Leukaemia Agent That Inhibits RNR and Suppresses Tumour Growth in Mouse Xenograft Model

Author

Dong, Jingwen, primary, Zhong, Tingting, additional, Xu, Zhijian, additional, Chen, Haiyi, additional, Wang, Xianjun, additional, Yang, Lili, additional, Lou, Zhiyuan, additional, Xu, Yuanling, additional, Hou, Tingjun, additional, Xu, Rongzhen, additional, Zhu, Weiliang, additional, and Shao, Jimin, additional

Published
2022
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16. Highly sensitive time‐resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco

Author

Gangling Tang, Zhang Haiyan, Hu Liwei, Zhaoyang Bian, Xiong Wei, Shao Jimin, Canhua Lu, Liu Shanshan, Yang Fei, Shili Liu, Yuan Ji, Dunhuang Fang, Shi Mowen, Huimin Deng, Fan Ziyan, and Wang Ying

Subjects
Chromatography, Chemistry, Fluoroimmunoassay, Alternaria longipes, Alternaria, Enzyme-Linked Immunosorbent Assay, General Medicine, Repeatability, Applied Microbiology and Biotechnology, Disease control, Highly sensitive, Brown spot, Tobacco, Biotechnology, Lateral flow immunoassay
Abstract

AIMS Alternaria longipes is a causal agent of brown spot of tobacco, which remains a serious threat to tobacco production. Herein, we established a detection method for A. longipes in tobacco samples based on the principle of time-resolved fluoroimmunoassay, in order to fulfil the requirement of rapid, sensitive and accurate detection in situ. METHODS AND RESULTS A monoclonal antibody against A. longipes was generated, and its purity and titration were assessed using western blot and ELISA. The size of europium (III) nanospheres was measured to confirm successful antibody conjugation. The method described here can detect A. longipes protein lysates as low as 0.78 ng ml-1 , with recovery rates ranging from 85.96% to 99.67% in spiked tobacco. The specificity was also confirmed using a panel of microorganisms. CONCLUSIONS The fluorescent strips allow rapid and sensitive onsite detection of A. longipes in tobacco samples, with high accuracy, specificity, and repeatability. SIGNIFICANCE AND IMPACT OF THE STUDY This novel detection method provides convenience of using crude samples without complex procedures, and therefore allows rapid onsite detection by end users and quick responses towards A. longipes, which is critical for disease control and elimination of phytopathogens.

Published
2021

18. Additional file 1 of Preclinical validation and phase I trial of 4-hydroxysalicylanilide, targeting ribonucleotide reductase mediated dNTP synthesis in multiple myeloma

Author

Xie, Yongsheng, Wang, Yingcong, Xu, Zhijian, Lu, Yumeng, Song, Dongliang, Gao, Lu, Yu, Dandan, Li, Bo, Chen, Gege, Zhang, Hui, Feng, Qilin, Zhang, Yong, Hu, Ke, Huang, Cheng, Peng, Yu, Wu, Xiaosong, Mao, Zhiyong, Shao, Jimin, Zhu, Weiliang, and Shi, Jumei

Abstract

Additional file 1.Supplementary Materials and Figures.

Published
2022
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21. Proteomics profiling of colorectal cancer progression identifies PLOD2 as a potential therapeutic target

Author

Shao, Yingkuan, primary, Xu, Kailun, additional, Zheng, Xi, additional, Zhou, Biting, additional, Zhang, Xiuli, additional, Wang, Lin, additional, Sun, Yaoting, additional, Li, Dan, additional, Chen, Ting, additional, Wang, Jian, additional, Yu, Shaojun, additional, Sun, Lifeng, additional, Xu, Xiaoming, additional, Dai, Shaozhi, additional, Gao, Huanhuan, additional, Ruan, Guan, additional, Liu, Wei, additional, Cai, Xue, additional, Zhu, Tiansheng, additional, Qi, Lina, additional, Chen, Jiani, additional, Hu, Wangxiong, additional, Weng, Xingyue, additional, Zhu, Yi, additional, Xiang, Xueping, additional, Hu, Zhiyuan, additional, Li, Jinfan, additional, Chen, Lirong, additional, Shao, Jimin, additional, Zheng, Shu, additional, and Guo, Tiannan, additional

Published
2021
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24. Highly sensitive time‐resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco

Author

Ji, Yuan, primary, Hu, Liwei, additional, Xiong, Wei, additional, Wang, Ying, additional, Yang, Fei, additional, Shi, Mowen, additional, Zhang, Haiyan, additional, Shao, Jimin, additional, Lu, Canhua, additional, Fang, Dunhuang, additional, Deng, Huimin, additional, Bian, Zhaoyang, additional, Tang, Gangling, additional, Liu, Shili, additional, Fan, Ziyan, additional, and Liu, Shanshan, additional

Published
2021
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25. Pterostilbene inhibits hepatocellular carcinoma proliferation and HBV replication by targeting ribonucleotide reductase M2 protein

Author

Wang, Rui, Zhijian Xu, Tian, Jiaping, Liu, Qian, Dong, Jingwen, Guo, Lijuan, Hai, Boning, Liu, Xia, Yao, Hangping, Chen, Zhi, Xu, Junjie, Zhu, Lijun, Chen, Haiyi, Hou, Tingjun, Zhu, Weiliang, and Shao, Jimin

Subjects
Original Article, neoplasms, digestive system diseases
Abstract

Hepatocellular carcinoma (HCC), one of the most deadly diseases all around the world. HBV infection is a causative factor of HCC and closely associated with HCC development. Ribonucleotide reductase (RR) is a key enzyme for cellular DNA synthesis and RR small subunit M2 (RRM2) is highly upregulated in HCC with poor survival rates. We have previously shown that HBV can activate the expression of RRM2 and the activity of RR enzyme for the viral DNA replication in host liver cells. Thus, RRM2 may be an important therapeutic target for HCC and HBV-related HCC. Pterostilbene, a natural plant component, potently inhibited in vitro RR enzyme activity with the IC(50) of about 0.62 μM through interacting with RRM2 protein, which was much higher than current RRM2 inhibitory drugs. Pterostilbine inhibited cell proliferation with an MTT IC(50) of about 20-40 μM in various HCC cell lines, causing DNA synthesis inhibition, cell cycle arrest at S phase, and accordingly apoptosis. On the other hand, the compound significantly inhibited HBV DNA replication in HBV genome integrated and newly transfected HCC cells, and the EC(50) for inhibiting HBV replication was significantly lower than the IC(50) for inhibiting HCC proliferation. Notably, pterostilbene possessed a similar inhibitory activity in sorafenib and lamivudine resistant HCC cells. Moreover, the inhibitory effects of pterostilbine against HCC proliferation and HBV replication were significantly reversed by addition of dNTP precursors, suggesting that RR was the intracellular target of the compound. Finally, pterostilbine effectively inhibited HCC xenograft growth with a relatively low toxicity in nude mouse experiments. This study demonstrates that pterostilbene is a novel potent RR inhibitor by targeting RRM2. It can simultaneously inhibit HCC proliferation and HBV replication with a potential new use for treatment of HCC and HBV-related HCC.

Published
2021

29. Determination of Commonly Used Multiclass Pesticide Residues in Tobacco and Cigarette Smoke by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry.

Author

Xiong, Wei, Tao, Xiaoqiu, Zhang, Haiyan, Huang, Mei, and Shao, Jimin

Subjects
LIQUID chromatography-mass spectrometry, PESTICIDE residues in food, PESTICIDE pollution, TOBACCO smoke, SOLID phase extraction, SMOKING, TOBACCO use
Abstract

A novel method has been developed for the simultaneous determination of multiclass pesticide residues in tobacco and cigarette smoke, using a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). Cigarette mainstream smoke particulate was collected on a Cambridge filter pad. Pesticide residues was extracted with an aqueous solution, back extracted into acetonitrile after freezing, purified by dispersive solid phase extraction with primary-secondary amine adsorbents and analyzed by UPLC–MS/MS. The obtained mean recoveries of 16 pesticides commonly used on tobacco at three fortification levels (5.9, 94.1 and 352.9 ng g−1) ranged from 69.3 to 115.9% with relative standard deviations between 2.4 and 11.3%. The limits of detection ranged from 0.14 to 13.28 ng g−1. Finally, the proposed method was applied to study the pesticide smoke transfer ratio in 2 cigarettes with pesticide standard spiked and 51 cigarettes with one or more pesticide residues. The transfer ratio of pesticides residue in tobacco into the smoke might be much less than that from artificially spiked tobacco (<25%) with spiking levels varied from 1.88 to 9.41 μg g−1. The transfer ratio of pesticide from artificially spiked tobacco into cigarette mainstream smoke was from 0.0 to 56.5%, and pesticide residues from tobacco into cigarette smoke were from 0.0 to 26.1% using the ISO smoking method (ISO 3308 2012). [ABSTRACT FROM AUTHOR]

Published
2022
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30. A rapid method for the simultaneous stereoselective determination of the triazole fungicides in tobacco by supercritical fluid chromatography-tandem mass spectrometry combined with pass-through cleanup

Author

Yang Fei, Yuan Ji, Xiaotao Zhang, Xiong Wei, Huimin Deng, Gangling Tang, Shao Jimin, Wang Ying, and Liu Shanshan

Subjects
Myclobutanil, Triazole, 010402 general chemistry, Tandem mass spectrometry, Mass spectrometry, 01 natural sciences, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Limit of Detection, Tandem Mass Spectrometry, Nitriles, Tobacco, Solid phase extraction, Tebuconazole, Detection limit, Chromatography, 010401 analytical chemistry, Organic Chemistry, Solid Phase Extraction, Reproducibility of Results, Chromatography, Supercritical Fluid, Stereoisomerism, General Medicine, Triazoles, 0104 chemical sciences, Fungicides, Industrial, chemistry, Calibration, Supercritical fluid chromatography, Solvents
Abstract

This work presents a simple, rapid and green chiral analysis method for five triazole fungicides (penconazole, tebuconazole, triadimefon, myclobutanil, and triadimenol) in tobacco, by which the samples were cleaned up by the novel pass-through solid phase extraction and subsequently the stereoisomers were separated and determined by the supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS). Optimized separation of the stereoisomers was achieved on an ACQUITY UPC2 Trefoil AMY 1 column within 6 min. Under fortified concentration levels of 0.1, 0.5 and 2.0 mg/kg, the mean recoveries were 82.8-106.6%, the intra-day relative standard deviations (RSDs) were 1.1-6.6%, and the inter-day RSDs were 2.5-5.6%. The correlation coefficient was greater than 0.9926 for all studied analytes within the range of 10-500 ng/mL. The limits of detection (LODs) for all stereoisomers ranged from 0.26 μg/kg to 3.24 μg/kg. The established method was subsequently successfully applied to analyze authentic samples, confirming that this method is a novel, rapid and environmentally friendly method for the stereoselective separation of triazole fungicides in tobacco.

Published
2020

31. Highly sensitive time‐resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco.

Author

Ji, Yuan, Hu, Liwei, Xiong, Wei, Wang, Ying, Yang, Fei, Shi, Mowen, Zhang, Haiyan, Shao, Jimin, Lu, Canhua, Fang, Dunhuang, Deng, Huimin, Bian, Zhaoyang, Tang, Gangling, Liu, Shili, Fan, Ziyan, and Liu, Shanshan

Subjects
FLUOROIMMUNOASSAY, ALTERNARIA, TOBACCO, IMMUNOASSAY, DISEASE eradication, WESTERN immunoblotting
Abstract

Aims: Alternaria longipes is a causal agent of brown spot of tobacco, which remains a serious threat to tobacco production. Herein, we established a detection method for A. longipes in tobacco samples based on the principle of time‐resolved fluoroimmunoassay, in order to fulfil the requirement of rapid, sensitive and accurate detection in situ. Methods and Results: A monoclonal antibody against A. longipes was generated, and its purity and titration were assessed using western blot and ELISA. The size of europium (III) nanospheres was measured to confirm successful antibody conjugation. The method described here can detect A. longipes protein lysates as low as 0.78 ng ml−1, with recovery rates ranging from 85.96% to 99.67% in spiked tobacco. The specificity was also confirmed using a panel of microorganisms. Conclusions: The fluorescent strips allow rapid and sensitive onsite detection of A. longipes in tobacco samples, with high accuracy, specificity, and repeatability. Significance and Impact of the Study: This novel detection method provides convenience of using crude samples without complex procedures, and therefore allows rapid onsite detection by end users and quick responses towards A. longipes, which is critical for disease control and elimination of phytopathogens. [ABSTRACT FROM AUTHOR]

Published
2022
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34. Integrative Analysis Reveals Across-Cancer Expression Patterns and Clinical Relevance of Ribonucleotide Reductase in Human Cancers

Author

Ding, Yongfeng, primary, Zhong, Tingting, additional, Wang, Min, additional, Xiang, Xueping, additional, Ren, Guoping, additional, Jia, Zhongjuan, additional, Lin, Qinghui, additional, Liu, Qian, additional, Dong, Jingwen, additional, Li, Linrong, additional, Li, Xiawei, additional, Jiang, Haiping, additional, Zhu, Lijun, additional, Li, Haoran, additional, Shen, Dejun, additional, Teng, Lisong, additional, Li, Chen, additional, and Shao, Jimin, additional

Published
2019
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37. Colorectal cancer-derived small extracellular vesicles establish an inflammatory premetastatic niche in liver metastasis

Author

Shao, Yingkuan, primary, Chen, Ting, additional, Zheng, Xi, additional, Yang, Sheng, additional, Xu, Kailun, additional, Chen, Xuewen, additional, Xu, Fei, additional, Wang, Lantian, additional, Shen, Yanwei, additional, Wang, Tingyang, additional, Zhang, Mengwen, additional, Hu, Wangxiong, additional, Ye, Chenyang, additional, Yu, XiaoFang, additional, Shao, Jimin, additional, and Zheng, Shu, additional

Published
2018
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41. JMJD5 cleaves monomethylated histone H3 N‐tail under DNA damaging stress

Author

Shen, Jing, primary, Xiang, Xueping, additional, Chen, Lihan, additional, Wang, Haiyi, additional, Wu, Li, additional, Sun, Yanyun, additional, Ma, Li, additional, Gu, Xiuting, additional, Liu, Hong, additional, Wang, Lishun, additional, Yu, Ying‐nian, additional, Shao, Jimin, additional, Huang, Chao, additional, and Chin, Y Eugene, additional

Published
2017
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42. Physical interaction between human ribonucleotide reductase large subunit and thioredoxin increases colorectal cancer malignancy

Author

Lou, Meng, primary, Liu, Qian, additional, Ren, Guoping, additional, Zeng, Jiling, additional, Xiang, Xueping, additional, Ding, Yongfeng, additional, Lin, Qinghui, additional, Zhong, Tingting, additional, Liu, Xia, additional, Zhu, Lijun, additional, Qi, Hongyan, additional, Shen, Jing, additional, Li, Haoran, additional, and Shao, Jimin, additional

Published
2017
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45. CREB1 directly activates the transcription of ribonucleotide reductase small subunit M2 and promotes the aggressiveness of human colorectal cancer

Author

Fang, Zejun, primary, Lin, Aifen, additional, Chen, Jiaoe, additional, Zhang, Xiaomin, additional, Liu, Hong, additional, Li, Hongzhang, additional, Hu, Yanyan, additional, Zhang, Xia, additional, Zhang, Jiangang, additional, Qiu, Lanlan, additional, Mei, Lingming, additional, Shao, Jimin, additional, and Chen, Xiang, additional

Published
2016
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48. IGF/STAT3/NANOG/Slug Signaling Axis Simultaneously Controls Epithelial-Mesenchymal Transition and Stemness Maintenance in Colorectal Cancer

Author

Yao, Chao, primary, Su, Li, additional, Shan, Juanjuan, additional, Zhu, Chuanlin, additional, Liu, Limei, additional, Liu, Chungang, additional, Xu, Yanmin, additional, Yang, Zhi, additional, Bian, Xiuwu, additional, Shao, Jimin, additional, Li, Jianming, additional, Lai, Maode, additional, Shen, Junjie, additional, and Qian, Cheng, additional

Published
2016
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49. Ribonucleotide reductase large subunit M1 plays a different role in the invasion and metastasis of papillary thyroid carcinoma and undifferentiated thyroid carcinoma

Author

Fang, Zejun, primary, Song, Rui, additional, Gong, Chaoju, additional, Zhang, Xiaomin, additional, Ren, Guoping, additional, Li, Jinfan, additional, Chen, Yuexia, additional, Qiu, Lanlan, additional, Mei, Lingming, additional, Zhang, Ronghui, additional, Xiang, Xueping, additional, Chen, Xiang, additional, and Shao, Jimin, additional

Published
2015
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