Search

Background N6-methyladenosine (m6A) is the most abundant internal modification in mammalian mRNAs. M6A is composed of methyl-transferases, which act as ("writers"), binding proteins, which act as ("readers"), and demethylases, which act as ("erasers"). As an important part of ("readers"), YTHDF1 and YTHDF3 were shown to be associated with many cancers. This paper aimed to study the expression of YTHDF1 and YTHDF3 in oral squamous cell carcinoma (OSCC). Methods We detected the expression of YTHDF1 and YTHDF3 in 120 OSCC patients by immunohistochemical analysis. Statistical analysis was used to determine whether the high or low expression of these two genes was significantly associated with age, gender, histological type, clinical stage, or lymph node metastasis. The correlation curve and survival curve of the two genes were produced to evaluate the potential clinical significance. Results We find the expression of YTHDF1 and YTHDF3 was increased in OSCC tissues compared to adjacent normal tissues. The statistical analysis showed that the expression of YTHDF1 and YTHDF3 was significantly associated with the clinical stage and histological type in OSCC patients. There was also a significant correlation between the expression of YTHDF1 and YTHDF3. A high expression of YTHDF1 and YTHDF3 was related to poor patient prognosis. Conclusions Our findings suggest that a high expression of YTHDF1 and YTHDF3 may be related to poor patient prognosis.

Background Sebaceous lymphadenoma is a benign tumor that occurs rarely in the salivary glands, most commonly in the parotid glands or periparotid lymph nodes, and even more rarely undergoes malignant transformation into a sebaceous lymphadenocarcinoma. Case summary We report an 82-year-old woman who presented with a painless mass in the right parotid region. We performed extended surgical resection of the parotid gland mass. Intraoperative pathology revealed a sebaceous lymphadenocarcinoma with metastasis into the periparotid cervical lymph nodes, so we also performed neck dissection and lymph node resection. Postoperative pathology confirmed the diagnosis. The literature review revealed that this was the seventh reported case of sebaceous lymphadenocarcinoma and the second reported case of cervical lymph node metastasis and infiltration of the skin of the parotid gland. Conclusion Treatment of sebaceous lymphadenocarcinoma depends on the typing and clinical staging of the cancer. Extensive resection is the first choice, and adjuvant radiotherapy should be given to patients with high-grade tumors or those at an advanced clinical stage.

In this study, a detailed modeling applicable for membrane dehumidifying system is proposed. This system contains a membrane module, 2-stage vacuum pumps, a condenser, and a regulating valve. The p...

Background N6-methyladenosine (m6A) is the most abundant internal modification in mammalian mRNAs. M6A is composed of methyl-transferases, which act as ("writers"), binding proteins, which act as ("readers"), and demethylases, which act as ("erasers"). As an important part of ("readers"), YTHDF1 and YTHDF3 were shown to be associated with many cancers. This paper aimed to study the expression of YTHDF1 and YTHDF3 in oral squamous cell carcinoma (OSCC). Methods We detected the expression of YTHDF1 and YTHDF3 in 120 OSCC patients by immunohistochemical analysis. Statistical analysis was used to determine whether the high or low expression of these two genes was significantly associated with age, gender, histological type, clinical stage, or lymph node metastasis. The correlation curve and survival curve of the two genes were produced to evaluate the potential clinical significance. Results We find the expression of YTHDF1 and YTHDF3 was increased in OSCC tissues compared to adjacent normal tissues。The statistical analysis showed that the expression of YTHDF1 and YTHDF3 was significantly associated with the clinical stage and histological type in OSCC patients. There was also a significant correlation between the expression of YTHDF1 and YTHDF3. A high expression of YTHDF1 and YTHDF3 was related to poor patient prognosis. Conclusions Our findings suggest that a high expression of YTHDF1 and YTHDF3 may be related to poor patient prognosis.

In this study, a quantitative method for classifying the frost geometry is first proposed to substantiate a numerical model in predicting frost properties like density, thickness, and thermal conductivity. This method can recognize the crystal shape via linear programming of the existing map for frost morphology. By using this method, the frost conditions can be taken into account in a model to obtain the corresponding frost properties like thermal conductivity, frost thickness, and density for specific frost crystal. It is found that the developed model can predict the frost properties more accurately than the existing correlations. Specifically, the proposed model can identify the corresponding frost shape by a dimensionless temperature and the surface temperature. Moreover, by adopting the frost identification into the numerical model, the frost thickness can also be predicted satisfactorily. The proposed calculation method not only shows better predictive ability with thermal conductivities, but also gives good predictions for density and is especially accurate when the frost density is lower than 125 kg/m3. Yet, the predictive ability for frost density is improved by 24% when compared to the most accurate correlation available.

To explore the effects of MTA, iRoot SP and AH Plus on periodontal ligament stem cells.The periodontal ligament stem cells were cloned by limiting dilution culture method. The effects of MTA, iRoot SP and AH Plus on proliferation and apoptosis of periodontal ligament stem cells were detected by MTT and Annexin-V-FITC/PI double staining. Alizarin and qRT-PCR were used to evaluate the effect of MTA, iRoot SP and AH plus on osteogenesis of periodontal ligament stem cells. SPSS 21.0 software package was used for statistical analysis.MTA showed mild toxicity at 24 and 48 hours, AH Plus showed mild toxicity at 24 h. iRoot SP was the least (P0.05) compared to MTA and AH Plus. The effect of three kinds of materials on apoptosis of periodontal ligament stem cells gradually decreased with the prolongation of time. Compared with the control group, the three kinds of materials were toxic at 3 d, the toxicity of MTA was the strongest and the toxicity of iRoot SP was the lowest(P0.05). Mineralization nodules in MTA and iRoot SP group were significantly higher than those in AH Plus and control group. The expression of OC, RUNX2, COL1A and ALP gene was higher at 7, 14, 21 d than in the control group and the expression of iRoot SP mineralization was the greatest(P0.05).The hardened iRoot SP is non-toxic to human periodontal ligament stem cells. Osteogenic ability and mineralization capacity of hardened iRoot SP on human periodontal ligament stem cells are better than MTA.

In this study, a review of the mathematical models and the CFD models to predict the frost formation on the flat plate are conducted and compared. The mathematical model is able to predict the critical frost properties like density, thickness, and thermal conductivity with less computational loading, but it cannot offer comprehensive information in the frost layer for each time step. The assumption of the absorption rate significantly influences the mass transfers and casts an impact on the calculated results accordingly. The supersaturation condition at the frost surface can mimic the actual situation, but it usually cannot be combined with the existing heat transfer correlations. The source term related to Gibbs free energy is depicted by the supersaturation degree, this perspective can be potentially expressed by the supersaturation degree proposed by the classical nucleation theory. Moreover, some CFD simulation models show good agreement with the experimental data, but the mass transfer calculation should be modified to yield better predictions as compared to the mathematical models and the coupling models. Besides, the ratio of the total mass flux between the mathematical model and the coupling model is in line with the ratio of the total heat transfer between the frosting condition and the non-frosting condition.

Autophagy, as an important non-selective degradation mechanism, could promote tumor initiation and progression by maintaining cellular homeostasis and the cell metabolism as well as cell viability. CircCDR1as has been shown to function as an oncogene in cancer progression, however, it remains largely unknown as to how autophagy is regulated by circCDR1as in oral squamous cell carcinoma (OSCC). In this study, we validated the functional roles of circCDR1as in regulation of autophagy in OSCC cells and further investigated how circCDR1as contributed to cell survival via up-regulating autophagy under a hypoxic microenvironment by using combination of human tissue model, in vitro cell experiments and in vivo mice model. We found that hypoxia promoted the expression level of circCDR1as in OSCC cells and elevated autophagy. In addition, circCDR1as further increased hypoxia-mediated autophagy by targeting multiple key regulators of autophagy. We revealed that circCDR1as enhanced autophagy in OSCC cells via inhibition of rapamycin (mTOR) activity and upregulation of AKT and ERK½ pathways. Overexpression of circCDR1as enhanced OSCC cells viability, endoplasmic reticulum (ER) stress, and inhibited cell apoptosis under a hypoxic microenvironment. Moreover, circCDR1as promoted autophagy in OSCC cells by sponging miR-671-5p. Collectively, these results revealed that high expression of circCDR1as enhanced the viability of OSCC cells under a hypoxic microenvironment by promoting autophagy, suggesting a novel treatment strategy involving circCDR1as and the inhibition of autophagy in OSCC cells.

To optimize reconstruction of maxillary unilateral defect by free fibula flap rapidly through 3D printing technique.3D models of reconstruction of unilateral maxillary defect by free fibula flap was established via CAD/CAM, metal customized plates and resin osteotomy guides were designed and manufactured via 3D printing technique, simulating operation was performed on models in vitro, and reconstructive accuracy after surgical simulation was analyzed using SPSS 18.0 software package.Digital models involving reconstructive bone and affiliated devices (plates and guides) were established and fabricated rapidly. There was no significant difference between simulating point on computer and surgical simulating point in vitro.The optimized design and customized plate can increase the accuracy of reconstruction of maxillary defects with free fibula in vitro, which is helpful for accurate surgical reconstruction.

Corn kernel moisture content is the most frequently used indicator for kernel maturity prediction and determination of the appropriate mechanized harvesting time. Corn varieties with lower moisture contents and thus faster dehydration of the corn kernels after physiological maturity are suitable for high plant density and mechanized harvesting. However, nondestructive measurement of the dynamic moisture content of the husk-covered corn kernel layer before physiological maturity in the field remains challenging at present. Therefore, in this study, a nondestructive measurement method based on the vector reflection coefficient measurement technique is proposed and developed for in situ monitoring of the husk-covered corn kernel layer moisture in the field. Based on the one-port calibration procedure used for the handheld moisture detection device, quadratic regression models were established for the corn kernel layer moisture using impedance parameters from 51 husk-covered corn samples. Results showed that this method enabled nondestructive estimation of the husk-covered corn kernel layer moisture ( R 2 = 0.62 ) with a 3.53% root mean square error ( RMSE ) using a serial capacitor C s and the husk moisture ( R 2 = 0.85 ) with a R M S E of 7.12%. Additionally, intact corn ears were measured in the field from the dent stage to the maturity stage using this device to investigate the dynamic moisture contents of the kernels and husks. Results showed that the measured moisture content of kernels before physiological maturity followed exponential decay functions with increasing numbers of days, and the mean difference between the measured kernel moisture contents of the intact corn ears and the oven-drying moisture contents of these kernels is 3.72%. In conclusion, the handheld moisture detection device allows us to measure the moisture of husk-covered corn kernel layers nondestructively before maturity in the field. This study thus provides a new way to obtain corn kernel dehydration curves in situ before maturity and estimate the corn maturity time.

To evaluate the clinical effects of functional neck dissection(FND) and radical neck dissection(RND) on early oral squamous cell carcinoma (OSCC).A total of 63 OSCC patients were divides into 2 groups. Patients in FND group (n=30) underwent functional neck dissection with preservation of sternocleidomastoid muscle, accessory nerve, internal jugular vein and great auricular nerve, while patients in RND group (n=33) underwent radical neck dissection. The follow-up data included movement of shoulder joint, great auricular nerve function, neck shape and local recurrent rate. SPSS 18.0 software package was used for data.There was no significant difference in age, gender, tumor location, T stage, N stage, histologic grades, pathological type, preoperative chemotherapy and postoperative chemotherapy between the two groups(P0.05). Cervical lymph nodes was not palpable(N0) or the diameter was less than or equal to 3cm(N1) for all patients. Compared to RND, the activities of shoulder joint point, earlobe numbness and neck concave deformity were significantly improved with FND(P0.05). Patients were followed up for 2 years, no significant difference was observed for neck recurrence between the two groups(P=1.000).FND can be safely performed in cN0 or cN1 OSCC patients, which avoids major complications with RND, and improves postoperative quality of life for OSCC patients with NO or N1 stage.

We theoretically investigate single-photon polarization conversion via scattering by an atom with Λ configuration coupled to a semi-infinite waveguide and discuss the two cases in which the Λ system is non-degenerated and degenerated. By applying the hard-wall boundary condition of the semi-infinite waveguide, it is found that single-photon polarization conversion can be realized with unit probability for both cases under the ideal condition. Together with the polarization conversion, the frequency conversion of a single photon can also be realized with unit probability in the ideal case if the Λ system is not degenerated.

Extrusion and molding is one of the most important manufacturing processes of short fiber-rubber composite material. In order to be used to tire tread, the short fibers in rubber are wanted to be radial orientated. But the orientation of short fibers depends on the die structure, and the performances of short fiber-rubber composite material depend on the radial orientation degree of short fibers. So, the die structure has been studied by experiments in order to get a proper die structure for manufacturing of short fiber-rubber composite material. The experimental results indicate that the proper die structure parameters are: the molding length is 15mm, the molding height is 9mm, the channel length is 75mm, the channel height is 12mm and the channel angle is 135º.

This paper explored the relationship among genetic distances, NIR spectra distances and NIR-based identification model performance of the seeds of maize inbred lines. Using 3 groups (total 15 pairs) of maize inbred lines whose genetic distaches are different as experimental materials, we calculates the genetic distance between these seeds with SSR markers and uses Euclidean distance between distributed center points of maize NIR spectrum in the PCA space as the distances of NIR spectrum. BPR method is used to build identification model of inbred lines and the identification accuracy is used as a measure of model identification performance. The results showed that, the correlation of genetic distance and spectra distancesis 0.9868, and it has a correlation of 0.9110 with the identification accuracy, which is highly correlated. This means near-Infrared spectrum of seedscan reflect genetic relationship of maize inbred lines. The smaller the genetic distance, the smaller the distance of spectrum, the poorer ability of model to identify. In practical application, near infrared spectrum analysis technology has the potential to be used to analyze maize inbred genetic relations, contributing much to genetic breeding, identification of species, purity sorting and so on. What's more, when creating a NIR-based identification model, the impact of the maize inbred lines which have closer genetic relationship should be fully considered.

On the basis of the research on the forming conditions and distribution of inorganic CO2 gas reservoirs and organic hydrocarbon gas reservoirs in the central uplift area of the Changling fault depression, the forming time, stage and sequence of the two gas reservoirs were studied and the pool-forming patterns were established by analyzing fluid inclusions and structural evolution. The organic gas is over-mature coal-derived gas, mainly from source rocks of the Yingcheng Formation, Shahezi Formation and Huoshiling Formation. Inorganic CO2 gas is mainly from mantle, secondarily from the degassing of volcanics. The distribution of gas-source fault systems and the degassing of mantle volcanos are the two main factors controlling the enrichment, migration and accumulation of inorganic CO2 gas reservoirs. The formation of hydrocarbon gas reservoirs is earlier than that of inorganic CO2 gas reservoirs, the former is 81(86 Ma during the depositional period of the Yaojia to early Nenjiang Formations, the latter is 28 Ma in the Late Paleogene. CO2 gas reservoirs mainly exist, alone or mixed with hydrocarbon gas, in the Yingcheng Formation volcanics, and the hydrocarbon gas reservoirs are on the top of the mixed gas reservoirs. Key words: Changling fault depression, hydrocarbon gas reservoir, inorganic CO2 gas reservoir, fluid inclusion, volcanic degassing

The composition and carbon isotope composition of about 150 natural gas samples reveal that natural gases of deep strata in Songliao Basin are derived from multiple sources and the coal-type gases are widely distributed in the basin. Several gas fields show features of coal-type gases or contain coal-type gases, with δ13C2 being larger than −28 ‰ and δ13CMCC6 being larger than −22‰, such as Shengping gas field in the north of the basin, Xiaohelong and Xiaochengzi gas fields in the south. Organic matter of source rocks in the deep strata is dominantly Type III, with a small portion of Type II. The average TOC of source rocks in the Shahezi (K1sh) Formation is 1.99%. Sedimentary facies studies of single wells, which penetrated coals of the Shahezi Formation, indicate that these coals are developed in peat swamps of deltaic plain, depressions between fan deltas, coastal shallow lakes, flooding plain etc, and structures are mainly distributed in the boundary of fault depressions, especially in their slope, some in the center of lakes. Key words: Songliao Basin, deep strata, coal-type gas, gas source rock, geochemical features, sedimentary environment

It is generally accepted that near infrared reflectance spectroscopy (NIRS) can be used to identify variety authenticity of bare maize seeds. In practical, maize seeds are covered with seed coating agents. Therefore it's of huge significance to investigate the feasibility of identifying coated maize seeds by NIRS. This study employed NIRS to quickly determine the variety of coated maize seeds. Influence of seed coating agent on NIR spectra was discussed. The NIR spectra of coated maize seeds were obtained using an innovative method to avoid the impact of the seed coating agent. Coated seeds were cut open, and the sections were scanned by the spectrometer, so as to acquire the information of the seed itself. Then, support vector machine (SVM), soft independent modeling of class analogy (SIMCA), and biomimetic pattern recognition (BPR) was employed to establish the identification model for four maize varieties, and yield 93%, 95.8%, 98% average correct rate respectively. BPR model showed better performance than SVM and SIMCA models. The robustness of identification model was tested by seeds harvested from four regions and model showed good performance.

An organocatalytic domino reaction between 2-arylideneindane-1,3-diones and glutaraldehyde has been devised that gives functionalized spirocyclohexane carbaldehydes with an all-carbon quaternary center. The reaction proceeds through a Michael/Aldol sequence in good-to-high chemical yields and with high levels of stereoselectivity (up to >95:5 dr and 99% ee) in the presence of the α,α-l-diphenylprolinol trimethylsilyl ether 3 (20 mol %) and DIPEA (20 mol %) in ether at 0 °C.

Oral squamous cell carcinoma (OSCC) is the most common head and neck malignancy. Here, we evaluated the expression of cold-inducible RNA-binding protein (CIRP) and toll-like receptors 4 (TLR4) in OSCC tissues with immunohistochemistry. Using biostatistical methods designed to assess the impact of the expression of CIRP and TLR4 on the prognosis of patients with OSCC and relate that expression to the clinicopathological characteristics of these patients. For the first time, we demonstrated that the expression of CIRP and TLR4 was increased in OSCC and that high levels of CIRP or TLR4 expression were associated with a short survival rate. In addition, we were surprised to find that the levels of expression of CIRP and TLR4 were very similar. The goal of this study was to evaluate whether these two genes may provide clues as to the regulatory mechanisms of OSCC, serve as prognostic markers and establish a new direction for further studies of these biological mechanisms.

An organocatalytic domino reaction between 2-arylideneindane-1,3-diones and glutaraldehyde has been devised that gives functionalized spirocyclohexane carbaldehydes with an all-carbon quaternary center. The reaction proceeds through a Michael/Aldol sequence in good-to-high chemical yields and with high levels of stereoselectivity (up to >95:5 dr and 99% ee) in the presence of the α,α-l-diphenylprolinol trimethylsilyl ether 3 (20 mol %) and DIPEA (20 mol %) in ether at 0 °C.

In modern society, more and more companies have introduced different systems in corporate business sectors, furthermore, an employee may work in various systems. As a result, the employees need to record multiple usernames and passwords, which brings discomfort and lots of likelihood of errors, and posts security risks for the different systems that have their own security user authentications. Meanwhile, most of management systems are based on B/S model, where one of the foremost requirements is to provide low-latency access to frequent and large amounts of requests. To address this needs, in this paper, we firstly demonstrate the design of a web unified user management system which based on the Role-based access control (RBAC) model and Memcached. Secondly we conducted an experiment on two groups of integrated prototype systems to validate our results. Finally we draw a conclusion than it can not only monitor users in real time and configure the permissions safely and flexibly, but also reduce the response time significantly and improve the system performance.

To assess the value of cerebral spinal fluid (CSF) and serum myelin basic protein (MBP) levels in the diagnosis of multiple sclerosis (MS).Enzyme-linked immunosorbent assay was used to detect the CSF and serum levels of MBP in patients with MS (n=45), patients with Guillain-Barre syndrome (GBS) (n=36) and control subjects (control) (n=33). The sensitivity and specificity of MBP in CSF and serum in the diagnosis of MS were evaluated using the receiver-operating characteristic (ROC) curves.The MBP levels in CSF and serum both increased significantly in MS group as compared with those in GBS (P0.01) and control groups (P0.01). The area under the curve (AUC) of the ROC curve of MBP in CSF was 0.853-/+0.037 for MS diagnosis, and with the optimal cut-off value of 0.87 pg/ml, CSF MBP showed a diagnostic sensitivity of 83.7% and specificity of 78.3%. The AUC of the ROC curve of serum MBP was 0.761-/+0.046, and the optimal cut-off value of 0.25 pg/ml resulted in a diagnostic sensitivity of 62.8% and specificity of 73.9%. No statistically significant difference was found between the two AUCs (P0.05).Evaluation of CSF and serum MBP levels allows accurate diagnosis of MS, and MBP level in the CSF has greater diagnostic sensitivity than serum MBP. The combination of both CSF and serum MBP levels may serve as a sensitive index for the diagnosis of MS.

This paper studied the effects of microbial agents Faby (MF) and Rongfeng (MR) on the dynamic changes of temperature, C/N ratio, NH(4)+ -N and NO(3)- -N contents and compost quality in the composting process of passion fruit marc. The results showed that both MF and MR could accelerate the composting process. Compared with the control, these two agents extended the sustaining time of high temperature from 4 days to 11 and 12 days, promoted the decrease of C/N ratio, and the NO(3)- -N concentration increased by 58.0% and 64.2%, respectively. After the amendment of MF or MR, the total N, P and K contents, total porosity and water-holding porosity in the compost increased significantly, while the bulk density decreased. No significant difference was observed in the effects of MR and MF on the high temperature composting process of passionflower fruit marc, but MF was more beneficial to the improvement of compost quality.

Objective: To explore the efficacy of He-Ne laser intravascular irradiation on infarction and hemorrheology. To observe the effects of intravascular low level He-Ne laser irradiation (ILLLI) of blood on cell proliferation, apoptosis and chromosome in lymphocyte from cerebral infarction. Methods: Seventy cases with cerebral infarction were randomly divided into groups control group (35 cases) treated only with common drugs and therapeutic group (35 cases) treated besides common drugs also by He-Ne laser intravascular irradiation. Their hemorrheology index and treatment results were observed and compared. The blood lymphocytes of cerebral infarction were cultured before and after treatment. After that, the mitosis index (MI), cell kinetics index (CKI), sister-chromatid exchanges (SCE) frequencies and apoptosis were determined. Results: The therapeutic group was better than the control one. The effective rate in the therapeutic group was 88.6%, in the control one was 65.7%. The viscosity and fibrinogen, etc. were better than that in the control group with significant difference (P 0.05) in cerebral infarction patients after treatment; The CKI of lymphocytes had no obvious difference among groups (P>0.05); SCE frequencies of lymphocytes had no statistic significance between control group and ILLLI on (P>0.05). It showed the apoptosis rate of lymphocytes in cerebral infarction patients after ILLLI treatment increased significantly compared with the control group, (P

Research of application with pressure controllable simple enemator for colon diseases in the course of diagnoses and treatment