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In this paper, we focus on multivariate doubly truncated first two moments of generalized skew-elliptical distributions. This class of distributions includes many useful distributions, such as skew-normal, skew Student-[Formula: see text], skew-logistic and skew-Laplace-normal distributions, as special cases. The formulas of multivariate doubly truncated covariance (MDTCov) for generalized skew-elliptical distributions are also given. Further, we compute multivariate doubly truncated expectations (MDTEs) and MDTCovs for [Formula: see text]-variate skew-normal, skew-Student-[Formula: see text], skew-logistic and skew-Laplace-normal distributions, and use Monte-Carlo method to simulate and compare with the above results. As applications, the results of multivariate tail conditional expectation (MTCE) and multivariate tail covariance (MTCov) for generalized skew-elliptical distributions are derived. In addition, an optimal problem involving MDTE and MDTCov risk measures is proposed. Finally, we use real data to fit skew-normal distribution and to discuss MTCEs and MTCovs of logarithm of adjusted prices for two portfolios consisting of three companies from S&P (Standard & Poor’s) sectors.

In this paper, we considered the condition number theory of a new generalized ridge regression model. The explicit expressions of different types of condition numbers were derived to measure the ill-conditionness of the generalized ridge regression problem with respect to different circumstances. To overcome the computational difficulty of computing the exact value of the condition number, we employed the statistical condition estimation theory to design efficient condition number estimators, and the numerical examples were also given to illustrate its efficiency.

Tilt-to-length (TTL) coupling noise arises from angular misalignments of interfering beams in optical path length (OPL) measurements and significantly impacts the accuracy of interferometry measurement systems. This paper focuses on geometric TTL coupling in a test mass (TM) interferometer and examines how an imaging system influences TTL noise suppression. First, the analytical expression of the geometric TTL coupling in a TM interferometer with alignment errors is derived and confirmed through numerical simulation. Subsequently, an imaging system is incorporated into the geometric model and the corresponding analytical expressions are obtained under two common conjugate relationships. Nevertheless, the TTL coupling remains beyond the requirement of TM interferometer, as the residual TTL coupled with alignment errors persists even with the imaging system. Therefore, an optimal position of the imaging system capable of eliminating the second-order term of the TTL coupling is determined. Meanwhile, the first-order term can be mitigated through in-orbit calibrations. These findings offer valuable guidance for the design and adjustment of imaging systems in space-borne gravitational wave detection missions, which require high-precision laser interferometry.

Abstract Background Porphyromonas gingivalis (Pg) is one of the keystone pathogens involved in periodontitis. The present study aimed to observe the relationship among different infection forms of Pg, systemic inflammation, and acute myocardial infarction (AMI). Methods A total of 382 patients diagnosed with AMI and 78 patients without coronary heart disease (CHD) were included in the study. DNA from exfoliated oral cells, circulating cell-free DNA (cfDNA), and genomic DNA (gDNA) from blood samples were extracted. The qPCR method was employed to detect Pg infection. Clinical characteristics, inflammatory parameters, and severity of coronary artery lesions of the patients were analyzed and compared. Results Both the oral colonization and distant invasion of Pg correlated positively with systemic inflammation. Multivariate logistic regression analysis suggested that Pg positivity in gDNA was correlated with the risk of AMI [Model 1 (odds ratio (OR) = 1.917, 95% confidence interval (CI) 1.108–3.315), Model 2 (OR = 1.863, 95% CI 1.064–3.262), and Model 3 (OR = 1.853, 95% CI 1.042–3.295); p

A kind of full-function two-sided optical bench interferometer (OBI) is designed to meet the practical requirements of the Taiji Program for space gravitational wave detection. The main optical paths are arranged on the A-side for transmission and interference, and other optical paths and electronic devices are placed on the B-side. According to the design scheme, we successfully constructed two OBIs by using hydrogen–oxygen catalytic stress-free bonding technology. When the OBI is installed and adjusted, the position and Angle error of the interference beam are controlled within 30 μm and 50 μrad through the self-designed precision mechanical clamping mechanism and beam position measuring device. The built OBI was placed on the vibration isolation platform in the vacuum tank for the stability test. The test results show that the noise of the OBI is less than 10 pm/√Hz in the frequency band of 0.1 Hz to 1 Hz, which meets the noise budget requirements of the Taiji Pathfinder in the middle- and high-frequency band.

DNA synthesis catalyzed by DNA polymerase is essential for all life forms, and phosphodiester bond formation with phosphorus center inversion is a key step in this process. Herein, by using a single-selenium-atom-modified dNTP probe, we report a novel strategy to visualize the reaction stereochemistry and catalysis. We capture the before- and after-reaction states and provide explicit evidence of the center inversion and in-line attacking SN2 mechanism of DNA polymerization, while solving the diastereomer absolute configurations. Further, our kinetic and thermodynamic studies demonstrate that in the presence of Mg2+ ions (or Mn2+), the binding affinity (Km) and reaction selectivity (kcat/Km) of dGTPαSe-Rp were 51.1-fold (or 19.5-fold) stronger and 21.8-fold (or 11.3-fold) higher than those of dGTPαSe-Sp, respectively, indicating that the diastereomeric Se-Sp atom was quite disruptive of the binding and catalysis. Our findings reveal that the third metal ion is much more critical than the other two metal ions in both substrate recognition and bond formation, providing insights into how to better design the polymerase inhibitors and discover the therapeutics.

In space gravitational wave detection missions, a drag-free system is used to keep the test mass (TM) free-falling in an ultralow-noise environment. Ground verification experiments should be carried out to clarify the shielding and compensating capabilities of the system for multiple stray force noises. A hybrid apparatus was designed and analyzed based on the traditional torsion pendulum, and a technique for enhancing the sensitivity of the torsion pendulum system by employing the differential wavefront sensing (DWS) optical readout was proposed. The readout resolution experiment was then carried out on an optical bench that was designed and established. The results indicate that the angular resolution of the DWS signal in optical readout mode can reach the level of 10 nrad/Hz1/2 over the full measurement band. Compared with the autocollimator, the sensitivity of the torsional pendulum is noticeably improved, and the background noise is expected to reach 4.5 × 10−15 Nm/Hz1/2@10 mHz. This method could also be applied to future upgrades of similar systems.

High-precision inertial sensors or accelerometers can provide references for free-falling motion in gravitational fields in space. They serve as the key payloads for gravity recovery missions such as CHAMP, the GRACE-type missions, and the planned Next-Generation Gravity Missions. In this work, a systematic method for electrostatic inertial sensor calibration of gravity recovery satellites is suggested, which is applied to and verified with the Taiji-1 mission. With this method, the complete operating parameters including the scale factors, the center of mass offset vector, and the intrinsic biased acceleration can be precisely calibrated with only two sets of short-term in-orbit experiments. This could reduce the gaps in data that are caused by necessary in-orbit calibrations during the lifetime of related missions. Taiji-1 is the first technology-demonstration satellite of the “Taiji Program in Space”, which, in its final extended phase in 2022, could be viewed as operating in the mode of a high–low satellite-to-satellite tracking gravity mission. Based on the principles of calibration, swing maneuvers with time spans of approximately 200 s and rolling maneuvers for 19 days were conducted by Taiji-1 in 2022. Given the data of the actuation voltages of the inertial sensor, satellite attitude variations, precision orbit determinations, the inertial sensor’s operating parameters are precisely re-calibrated with Kalman filters and are relayed to the Taiji-1 science team. The relative errors of the calibrations are

This study aims to identify the effect of uncertainty on the dynamic characteristics of a thin-walled hollow spherical rubber isolator. Firstly, the nonlinearity and uncertainties of the spherical rubber isolator were obtained by swept-sine experiments. The relationship between dynamic stiffness and damping against relative displacement amplitude was established. Then, a high-order polynomial function with uncertain parameters introduced was used for simulation. The uncertain parameters were quantified using the singular value decomposition and Monte Carlo simulations. Furthermore, the dynamic stiffness and damping of the experimental data were optimized using the Covariance Matrix Adaptation and Evolution Strategy. Finally, the high consistency between the simulation results and experimental results were demonstrated. Overall, the nonlinear model established according to the uncertain parameter quantization is feasible. This method can predict the nonlinear characteristics of the rubber isolator well and also may be applied to other nonlinear models.

The micro capacitive sensing and electrostatic drive control system (front-end electronics, FEE) is the core component of inertial sensor in space gravitational wave detection. The FEE requires high-precision displacement detection, high-stability electrostatic drive, and stable system control to achieve an acceleration resolution of 10-15 m/s2/Hz1/2 in the low-frequency range of 0.1 mHz-1 Hz. Based on the requirements of the future Chinese space gravitational wave detection task (Taiji Program), this paper conducted key technical research of the FEE using differential capacitance detections and electrostatic drives. The structure and working principle of the FEE were also introduced. The structural parameters of the entire system, working parameters, and electrostatic control system model were provided, and the performance of the PID controller was analyzed. Finally, using the torsion pendulum to overcome the influence of gravity on the earth, the FEE multi-degree of freedom control function was verified on the vibration isolation marble platform, the measurement range and power conversion coefficient were calibrated, and the noise level under current conditions was measured. Experimental results show that the FEE developed in this paper can achieve stable control in multiple degrees of freedom, the acceleration range is larger than 10-3 m/s2, the electric force conversion factor is 4.8 × 10-3 m/s2/V, and the measured acceleration resolution is 9.6 × 10-6 m/s2/Hz1/2. After optimizing the sensitive structure parameters, the acceleration resolution can be estimated at 3.3 × 10-15 m/s2/Hz1/2. These results satisfy the Taiji Program requirements. This paper provides a solid foundation for the future exploration of space gravitational waves in China and clears the optimization direction for the next step.

Abstract The detection mission of gravitational waves in space is that the accuracy of the long-baseline intersatellite laser interferometry on the million-kilometer order needs to reach the order of $$8 \rm{pm}/\sqrt{\rm{Hz}}$$ 8 p m / H z . Among all noise sources that affect the interferometry accuracy, tilt-to-length (TTL) coupling noise is the second largest source of noise after shot noise. This paper focuses on studying the contribution of TTL coupling noise of the telescope system in the intersatellite scientific interferometer. By referring to the laser interferometer space antenna (LISA)’s noise budget, TTL coupling noise is required to be within ±25μm/rad (±300μrad). Therefore, this paper focuses on studying both the mechanism of TTL coupling noise due to the noise sources of the telescope and the method of suppressing the TTL noise, which can lay a foundation for noise distribution and the development of engineering prototypes in subsequent tasks.

In this study, we sought to investigate the potential application of γδ T cell-derived extracellular vesicles (γδTDEs) as drug delivery system (DDS) for miR-138 in the treatment of oral squamous cell carcinoma (OSCC). Our data showed that overexpression of miR-138 in γδ T cells obtained miR-138-rich γδTDEs accompanying increased expansion and cytotoxicity of γδ T cells. γδTDEs inherited the cytotoxic profile of γδ T cells and could efficiently deliver miR-138 to OSCC cells, resulting in synergetic inhibition on OSCC both in vitro and in vivo. The pre-immunization by miR-138-rich γδTDEs inhibited the growth of OSCC tumors in immunocompetent C3H mice, but not in nude mice, suggesting an immunomodulatory role by miR-13-rich γδTDEs. γδTDEs and miR-138 additively increased the proliferation, interferon-γ (IFN-γ) production, and cytotoxicity of CD8+ T cells against OSCC cells. Only delivered by γδTDEs can miR-138 efficiently target programmed cell death 1 (PD-1) and CTLA-4 in CD8+ T cells. We conclude that γδTDEs delivering miR-138 could achieve synergetic therapeutic effects on OSCC, which is benefited from the individual direct anti-tumoral effects on OSCC and immunostimulatory effects on T cells by both γδTDEs and miR-138; γδTDEs could serve as an efficient DDS for microRNAs (miRNAs) in the treatment of cancer. Keywords: extracellular vesicle, γδ T cell, miRNA, drug delivery system, oral squamous cell carcinoma

Objective To explore specific flora in mouse models of non-alcoholic steatohepatitis (NASH) to improve NASH diagnostic protocols. Methods Sixty mice were divided into normal diet (ND, 20 mice) and high-fat/high-sugar diet (HFSD) groups (40 mice). After 8 weeks of feeding, 10 mice in the ND group and 20 mice in the HFSD group were sacrificed to create the short-term ND and non-alcoholic fatty liver (NAFL) groups, respectively. After 16 weeks of feeding, the remaining mice were sacrificed to create the long-term ND and NASH groups, respectively. We then examined fecal flora, serum biochemical indices, and lipopolysaccharide and tumor necrosis factor-α levels and analyzed liver tissue. Results The relative abundance of Lactobacillus , Desulfovibrio , Ruminiclostridium 9, and Turicibacter differed between NASH and NAFL mice, and the areas under the receiver operating characteristic curve of the four genera for diagnosing NASH were 0.705, 0.734, 0.737, and 0.937. The non-alcoholic fatty liver disease activity score was positively correlated with the relative abundance of Desulfovibrio (r = 0.353), Ruminiclostridium 9 (r = 0.431), and Turicibacter (r = 0.688). Conclusions The relative abundance of Lactobacillus , Desulfovibrio , Ruminiclostridium , and Turicibacter may help distinguish NASH from NAFL.

This study aims to identify the fatigue lifetime of a thin-walled hollow spherical rubber isolator subjected to harmonic and random vibrations. Firstly, the nonlinear characteristics of the rubber materials are tested and analyzed through vibration experiments. The relationship among the nonlinear stiffness, nonlinear damping, and relative displacement amplitude is established. Secondly, an accelerated lifetime experiment under random excitation is carried out. The correlation between the root-mean-squared acceleration response of the random vibration system and the stiffness attenuation of the rubber isolator is established and modeled. A lifetime prediction method for rubber isolators is proposed based on the model. Finally, the influence of different loads on the lifetime of the rubber isolator is investigated. These methods provide an additional rationale for the further theoretical research and practical engineering application of rubber damping systems.

Abstract Normal cell cycle progression and proliferation of palatal mesenchymal cells are important for palatal development. As targets of miR-17-92, E2F transcription factors family has been suggested to induce the transcription of miR-17-92 in several cell types. In the present study, we sought to investigate whether this negative feedback loop exists in mouse PMCs and what the function of this negative feedback loop would be in palatal mesenchymal cells. Using GeneMANIA, we revealed that the most important function of experimentally verified targets of miR-17-92 is cell cycle regulation. E2F1 and E2F3, but not E2F2, were extensively expressed in mouse palate. Over-expression of E2F1 significantly increased the expression of all the members of miR-17-92. After increased by E2F1, miR-17 and miR-20a may negatively target E2F1, and thereby prevent the cells from excessive proliferation. We suggest that the negative feedback loop between E2F1 and miR-17-92 may contribute to palatal development by regulating the proliferation and cell cycle of palatal mesenchymal cells.

Children's language development can reflect the developmental process of children's cognition and social emotions. The present study focuses on preschool children's conversational competence and narrative competence, aiming at exploring developmental features of preschool children's pragmatic competence through analyzing data from self-built oral language corpora using the INCA-A coding system and CLAN. Results show that with respect to using pragmatic indicators, frequency increases significantly with age in the dimensions of knowledge and language. In the language dimension, frequency is highest in the middle group and in the behavior dimension, frequency gradually decreases. Results are taken to indicate that preschool children's language competence improves continually, and gradually displaces the use of behavior-assisted language. Meanwhile, children can initiate conversation or discourse narration by using knowledge and language. Based on the findings, this study analyzes the development of preschool children's pragmatic competence at different ages within the framework of perlocutionary acts. Finally, corresponding educational suggestions are suggested.

The inertial sensor is the key measurement payload of the technology verification satellite of China’s space gravitational wave detection mission-Taiji Project, which uses capacitive sensors to sense the acceleration disturbance of the test mass under the influence of non-conservative forces in the frequency range of 10 mHz~1 Hz. It is necessary to perform a ground performance evaluation and estimate the working state of the payload in orbit. However, due to the influence of the earth’s gravity and seismic noise, it is impossible to directly evaluate the resolution level of the non-sensitive axis when testing with high-voltage levitation, which leads to incomplete evaluation of the performance of the inertial sensor. In order to implement this part of the test, the sensitive structure is designed and a torsion pendulum facility for performance testing is developed. The experimental results show that the measurement resolution of the non-sensitive axis of the inertial sensor can reach 9.5 × 10−7 m/s2/Hz1/2 under the existing ground environmental conditions and is mainly influenced by the seismic noise during the system measurement. If the inertial sensor enters orbit, the measurement resolution can achieve 3.96 × 10−9 m/s2/Hz1/2, which meets the requirements of the technology verification satellite for a non-sensitive axis. This proposed system also provides a reasonable method for the comprehensive evaluation of inertial sensors in the future.

Background: Long-term outcomes of drug-eluting stents (DES) versus bare-metal stents (BMS) in patients with ST-segment elevation myocardial infarction (STEMI) remain uncertain. Objective: To investigate long-term outcomes of drug-eluting stents (DES) versus bare-metal stents (BMS) in patients with ST-segment elevation myocardial infarction (STEMI). Methods: We performed search of MEDLINE, EMBASE, the Cochrane library, and ISI Web of Science (until February 2013) for randomized trials comparing more than 12-month efficacy or safety of DES with BMS in patients with STEMI. Pooled estimate was presented with risk ratio (RR) and its 95% confidence interval (CI) using random-effects model. Results: Ten trials with 7,592 participants with STEMI were included. The overall results showed that there was no significant difference in the incidence of all-cause death and definite/probable stent thrombosis between DES and BMS at long-term follow-up. Patients receiving DES implantation appeared to have a lower 1-year incidence of recurrent myocardial infarction than those receiving BMS (RR = 0.75, 95% CI 0.56 to 1.00, p= 0.05). Moreover, the risk of target vessel revascularization (TVR) after receiving DES was consistently lowered during long-term observation (all p< 0.01). In subgroup analysis, the use of everolimus-eluting stents (EES) was associated with reduced risk of stent thrombosis in STEMI patients (RR = 0.37, p=0.02). Conclusions: DES did not increase the risk of stent thrombosis in patients with STEMI compared with BMS. Moreover, the use of DES did lower long-term risk of repeat revascularization and might decrease the occurrence of reinfarction.

A series of CeOx catalysts supported by commercial porous cordierite ceramics (CPCC) and synthesized porous cordierite ceramics (SPCC) from fly ash were prepared for selective catalytic reduction of NOx with ammonia (NH3-SCR). A greater than 90% NOx conversion rate was achieved by the SPCC supported catalyst at 250−300 °C when the concentration of loading precursor was 0.6 mol/L (denoted as 0.6Ce/SPCC), which is more advantageous than the CPCC supported ones. The EDS mapping results reveal the existence of evenly distributed impurities on the surface of SPCC, which hence might be able to provide more attachment sites for CeOx particles. Further measurements with temperature programmed reduction by hydrogen (H2-TPR) demonstrate more reducible species on the surface of 0.6Ce/SPCC, thus giving rise to better NH3-SCR performance at a low-temperature range. The X-ray photoelectron spectroscopy (XPS) analyses reveal that the Ce atom ratio is higher in 0.6Ce/SPCC, indicating that a higher concentration of catalytic active sites could be found on the surface of 0.6Ce/SPCC. The in situ diffused reflectance infrared fourier transform spectroscopy (DRIFTS) results indicate that the SCR reactions over 0.6Ce/SPCC follow both Eley-Rideal (E-R) and Langmuir-Hinshelwood (L-H) mechanisms. Hence, the SPCC might be a promising candidate to provide support for NH3-SCR catalysts, which also provide a valuable approach to recycling the fly ash.