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To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.cell.2010.10.033 Byline: Liat Harel-Sharvit (1), Naama Eldad (1), Gal Haimovich (1), Oren Barkai (1), Lea Duek (1), Mordechai Choder (1) Keywords: RNA; CELLBIO Abstract: Little is known about crosstalk between the eukaryotic transcription and translation machineries that operate in different cell compartments. The yeast proteins Rpb4p and Rpb7p represent one such link as they form a heterodimer that shuttles between the nucleus, where it functions in transcription, and the cytoplasm, where it functions in the major mRNA decay pathways. Here we show that the Rpb4/7 heterodimer interacts physically and functionally with components of the translation initiation factor 3 (eIF3), and is required for efficient translation initiation. Efficient translation in the cytoplasm depends on association of Rpb4/7 with RNA polymerase II (Pol II) in the nucleus, leading to a model in which Pol II remotely controls translation. Hence, like in prokaryotes, the eukaryotic translation is coupled to transcription. We propose that Rpb4/7, through its interactions at each step in the mRNA lifecycle, represents a class of factors, 'mRNA coordinators,' which integrate the various stages of gene expression into a system. Author Affiliation: (1) Department of Molecular Microbiology, Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, 31096, Israel Article History: Received 17 December 2009; Revised 28 July 2010; Accepted 25 October 2010 Article Note: (miscellaneous) Published: November 11, 2010

A subunit of RNA polymarase II, Rpb4p, is required for the decay of a class of mRNAs whose products are involved in protein synthesis. Rpb4p promotes the deadenylation process of specific mRNAs and recruits Pat1/Lsm1-7 to mRNAs, thus stimulating their decapping and further decay.