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ObjectiveWe conducted a drug resistance and homology analysis of diarrheagenic Escherichia coli （DEC） in Fengxian District of Shanghai in order to provide a basis for clinical rational drug use， risk monitoring and early warning.MethodsDEC were isolated from diarrheal patients in Fengxian District， Shanghai from 2019 to 2022. The minimum inhibitory concentrations （MIC） of 21 drugs to the DEC were determined. Genotyping and homology analysis were conducted with pulsed-field gel electrophoresis （PFGE）.ResultsThe DEC detection rate of diarrhea cases was 18.99% （131/690）， including enteroaggregative E.coli （EAEC） 64.89% （85/131）， enterotoxigenic E.coli （ETEC） 22.14% （29/131）， enteropathogenic E.coli （EPEC） 12.21% （16/131）， and enterohemorrhagic E.coli （EHEC） 0.76%（1/131）. The DEC detection showed obvious seasonal characteristics with a high incidence in summer. The DEC multidrug resistance rate was 66.41% with a total of 65 drug resistance profiles. The five antimicrobial drugs with the highest resistance rate were ampicillin （60.31%）， nalidixic acid （51.91%）， cefazolin （50.38%）， tetracycline （44.27%）， and cotrimoxazole （35.11%）. The rate of DEC resistance to levofloxacin was significantly increased from 2019 to 2022. Cluster analysis showed that the similarity of 85 EAEC cluster was 58.4%‒100.0%， and 69 band patterns were obtained. The similarity of 29 ETEC cluster was 58.5%‒100.0%， and 13 band patterns were obtained， including 2 dominant band types. The similarity of 16 EAEC clusters was 53.9%‒100.0%， and 15 band patterns were obtained. Five groups of homologous strains were found， consistent with the resistance phenotypes.ConclusionAmong the diarrhea cases， the DEC epidemic intensity is high， the drug resistance situation is severe， and the risk of outbreak infection is high in Fengxian District， Shanghai. Therefore， health monitoring and prevention need to be strengthened.

ObjectiveTo determine the drug resistance， main virulence genes and molecular typing of Vibrio parahaemolyticus from multiple sources in Fengxian District， Shanghai.MethodsA total of 210 strains of Vibrio parahaemolyticus were isolated in Fengxian District， Shanghai， from 2018 to 2020. Then drug susceptibility tests of 17 antibiotics， virulence gene detection of thermolabilehemolysin （tlh）， thermostable direct hemolysin （tdh） and thermostable direct hemolysin-relatedhemolysin （trh）， and pulsed-field gel electrophoresis （PFGE） were conducted.ResultsA total of 147 drug-resistant strains were detected， with the drug resistance of 70.00%. Among them， 126 strains were resistant to single antibiotic， mainly resistant to CFZ； 15 strains were resistant to two antibiotics， mainly resistant to CFZ and AMP； and 6 strains of MDR， mainly resistant to AMP， SXT， CFZ， CTX， CAZ and FEP. The AMP resistance of food-borne strains was higher than that of human-derived strains （P

This article examines both industry-created and viewer-created paratexts of Disney’s new Mulan (2020) to shed light on the localization of industry-created paratexts for Chinese audiences, the reception of the film, and the tensions between industry-created and viewer-created paratexts in China. It reveals that Disney has localized the poster, trailers and promos of Mulan for Chinese audiences by highlighting the film’s all-star cast and creating an illusion of its faithfulness to The Ballad of Mulan, and the extolled virtues of filial piety and patriotism cherished in Chinese culture. The viewer-created paratext, danmu, of Mulan challenges the industry-created paratexts and demonstrates unfavorable reception of the film in China. It criticizes the film for its inauthenticity to The Ballad of Mulan, cultural appropriation, promotion of American values to meet the needs of English-speaking audiences for exoticness and cultural otherness. By investigating danmu as a viewer-created paratext, the present study has enriched paratextual research and reception studies in translation, media and film studies in the digital age. [ABSTRACT FROM AUTHOR]

Hybrid solid electrolytes (HSEs) have attracted much attention due to their advantages as both inorganic and organic polymer electrolytes. However, the organic/inorganic interfacial space charge layer has a great barrier to the transport of Li+ in the HSE. Here, an in situ polymerization layer is proposed on garnet‐type particles, working as the coherent region to eliminate the space charge layer at the organic/inorganic interfaces by inhibiting electron localization. The conjugate hybridization of fillers weakens the aggregation of particles, induces the dissociation of Li salt, and provides high‐throughput Li+ transport pathways at the ceramics/polymer interface. Furthermore, the continuous Li+ conduction networks are connected by the coherent region between inorganic fillers and polymer chains. The fabricated HSE exhibits a high ionic conductivity of 0.47 mS cm−1 and ion migration numbers of 0.78 at room temperature. The 3D Li//Li systematic battery assembled with the HSE delivers a high critical current density (CCD) of 2.0 mA cm−2. Meanwhile, the 4.5 V NCM811//Li batteries achieve a prolonged operation of 500 cycles at 0.5 C. The Li//LiFePO4 batteries demonstrate superior capacity retention of 96.4% at 1 C after 500 cycles. [ABSTRACT FROM AUTHOR]

The severe shuttle effect of polysulfides and sluggish redox kinetics are the main problems that hinder the practical applications of lithium–sulfur (Li–S) batteries. In this study, dual metallic single atom pairs are implanted into carbon nanospheres to address the shuttle effect and facilitate the bi‐directional redox kinetics of sulfur. Fe sites are found to be favorable for the reduction reaction of polysulfides, while Co sites facilitate the dissolution of Li2S experimentally and theoretically, promoting the overall electrochemical performance of Li−S batteries due to the synergetic effect. The sulfur cathode with the dual metallic single atom pairs exhibits an ultrahigh capacity retention of 96.4% after 100 cycles at 0.5 C. More importantly, it demonstrates excellent rate performance (618 mAh g−1 at current density of 10 C) and outstanding cycle performance at 2 C after 2800 cycles with only 0.01% decay per cycle. Impressively, the assembled pouch cell delivers a high discharge capacity of 1385 mAh g−1 and retains 869 mAh g−1 after 100 cycles at 0.2 C. These results demonstrate the potential of dual single atom catalysts for the favorable bi‐directional redox kinetics in Li−S batteries. [ABSTRACT FROM AUTHOR]