18 results on '"Sheng-Ying Li"'
Search Results
2. Identification and characterization of a novel hydroxylamine oxidase, DnfA, that catalyzes the oxidation of hydroxylamine to N2
- Author
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Meng-Ru Wu, Li-Li Miao, Ying Liu, Xin-Xin Qian, Ting-Ting Hou, Guo-Min Ai, Lu Yu, Lan Ma, Xi-Yan Gao, Ya-Ling Qin, Hai-Zhen Zhu, Lei Du, Sheng-Ying Li, Chang-Lin Tian, De-Feng Li, Zhi-Pei Liu, and Shuang-Jiang Liu
- Subjects
Cell Biology ,Molecular Biology ,Biochemistry - Published
- 2022
3. Fluorescence emission properties of rhodamine B encapsulated organic-inorganic hybrid mesoporous silica host
- Author
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Xinzhen Du, Sheng-Ying Li, Zhonghua Xue, Guohu Zhao, and Honghong Rao
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Materials science ,Absorption spectroscopy ,02 engineering and technology ,Mesoporous silica ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Mesoporous organosilica ,chemistry.chemical_compound ,chemistry ,MCM-41 ,Materials Chemistry ,Ceramics and Composites ,Rhodamine B ,Absorption (chemistry) ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Mesoporous material - Abstract
Mesoporous silica material can be viewed as an ideal solid host for constructing desirable dye laser with tunable wavelength range. In this investigation, Rhodamine B molecules were successfully encapsulated into the pore of highly ordered MCM-41 and Phenyl-functionalized MCM-41 mesoporous silica by using a simple and facile postgrafting method. The as-gained Rhodamine B encapsulated mesoporous silica materials were successfully characterized by Fourier transform infrared spectroscopy, nitrogen adsorption-desorption and small angle X-ray diffraction. Particularly, the optical absorption and electrochemical behaviors investigated by UV-vis absorption spectra and electrochemical cyclic voltammogram indicate that the difference on the hydrophillic nature of different mesoporous silica hosts has great influence on the existence state of Rh B molecules, resulting lead to strong fluorescence and obvious blue-shift of the encapsulated Rhodamine B molecules in the proposed organic-inorganic hybrid mesoporous. Furthermore, it was pointed out that the significant host-guest interactions of mesoporous silica and Rhodamine B are related not only to the structure, but also to the environmental and surrounding polarity of the materials.
- Published
- 2016
4. The extracts of Lobaria pindarensis, an edible lichen from Himalayas, suppress inflammatory response and fibrillation of amyloid β-protein in cultured microglial cells: Active depsidones, norstictic acid and stictic acid, are responsible for the functions
- Author
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Mei-Xia Yang, Edwin Hok-Chi Cheng, Dusadee Ospondpant, Ka-Ki Tung, Li-Song Wang, Sheng-Ying Lin, Queenie Wing-Sze Lai, Qi-Yun Wu, Ying-Jie Xia, Ka-Wing Leung, Ran Duan, Tina Ting-Xia Dong, and Karl Wah-Keung Tsim
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Alzheimer’s disease ,Inflammation ,Fibrillar Aβ ,Lichen ,Lobaria pindarensis ,Depsidone ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Lobaria pindarensis Räsänen is an edible and medicinal lichen found in the Himalayas that has been used as ethnomedicine for years to combat inflammation. The study aimed to evaluate the ability of L. pindarensis extracts and its main depsidones to suppress inflammation and Aβ fibril formation in cultured cells induced by LPS or Aβ42. The mRNA and protein expressions of inflammatory cytokines were declined significantly by the applied herbal extracts and depsidones in the LPS/Aβ-treated immune cells (p
- Published
- 2023
- Full Text
- View/download PDF
5. [Detection and analysis of bocavirus in hospitalized children with respiratory infection]
- Author
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Wei, Wang, Shu-Xiang, Lin, Sheng-Ying, Li, Xiao-Ju, Hou, Jing-Fu, Huang, Yi-Min, Zhu, and Hong-Jiang, Yang
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Bocavirus ,Male ,Child, Preschool ,论著·临床研究 ,Humans ,Infant ,Female ,Seasons ,Child, Hospitalized ,Polymerase Chain Reaction ,Respiratory Tract Infections ,Phylogeny - Abstract
OBJECTIVE: To detect human bocavirus (HBoV) and investigate its genetic and evolutionary characteristics in children with acute respiratory infection in Tianjin, China. METHODS: A total of 1 259 samples of nasopharyngeal aspirates were collected from children with a confirmed diagnosis of acute respiratory infection between January and December, 2012. Viral nucleic acid was extracted, HBoV was detected by real-time quantitative PCR, and the gene segments of nucleocapsid protein of HBoV in positive samples were amplified by PCR. Several products were randomly selected and sequenced.The sequence obtained was compared with the known sequence of HBoV, and a phylogenetic analysis was performed. All the samples were examined to detect for other common respiratory tract viruses. RESULTS: Among the 1 259 samples, the positive rate of HBoV was 4.53% (57/1 259), and among the 57 samples with positive HBoV, 75% (43/57) were positive in children with an age of 6-36 months. The positive rate of HBoV in children peaked in summer (from June to August), and there was a mixed infection with other viruses. Sequence analysis was performed for the PCR products from 36 positive samples, and the presence of HBoV was confirmed, with a higher homology to the known sequence of HBoV. CONCLUSIONS: In Tianjin, acute respiratory infection in some children may be associated with HBoV infection, which is commonly seen in infants with an age of 6-36 months. The peak of HBoV infection occurs in summer. The phylogenetic analysis shows a high homology to the known sequence of HBoV, with few gene sequence variations.
- Published
- 2016
6. Evaluation on pore structures of organosilicate thin films by grazing incidence small-angle x-ray scattering
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Hong-Ji Chen, Sheng-Ying Li, Rui-Peng Li, Smilgies, Detlef-M., Zhong-Hua Wu, and Zhihong Li
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Dielectric films -- Structure ,Dielectric films -- Chemical properties ,Dielectric films -- Optical properties ,Thin films -- Structure ,Thin films -- Chemical properties ,Thin films -- Optical properties ,Organosilicon compounds -- Structure ,Organosilicon compounds -- Chemical properties ,Organosilicon compounds -- Optical properties ,Chemicals, plastics and rubber industries - Published
- 2009
7. Improving the photocatalytic performance of conducting polymer polythiophene sensitized TiO2 nanoparticles under sunlight irradiation
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Shi Hong Xu, Li Zhang, Fei Xu, Sheng Ying Li, and Yun Xia Wei
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Conductive polymer ,Nanocomposite ,Materials science ,Photochemistry ,Chloride ,Catalysis ,chemistry.chemical_compound ,chemistry ,Titanium dioxide ,Methyl orange ,Photocatalysis ,medicine ,Polythiophene ,Physical and Theoretical Chemistry ,medicine.drug - Abstract
Titanium dioxide (TiO2), polythiophene and polythiophene/TiO2 were prepared by sol–gel and solid-state reaction methods. Water-free iron(III) chloride (FeCl3) was used as an oxidant. The phase composition, morphology and the spectral properties of the products were characterized by XRD, TEM, UV–Vis and FT-IR techniques. The photocatalytic activity of the products was evaluated by the degradation of methyl orange under sunlight irradiation. TEM results showed that the polythiophene/TiO2 composite particles were well dispersed, rod-like shaped with 20 × 80 nm dimensions. UV–Vis analysis indicated that the absorption edge of polythiophene/TiO2 was 605 nm. Compared with the unmodified TiO2 and bare polythiophene, polythiophene/TiO2 exhibited largely enhanced activity for the photocatalytic degradation of methyl orange under sunlight irradiation. A degradation efficiency of methyl orange of 85.6% could be obtained within 120 min. The sensitization mechanism of polythiophene for the TiO2 photocatalyst is discussed briefly.
- Published
- 2010
8. Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression
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Lei Wang, Sheng‑Ying Li, Ming‑Li Ji, Zhi‑Bing Qian, and Yu‑Xia Wang
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Cancer Research ,Pathology ,medicine.medical_specialty ,Thymoma ,Oncogene ,medicine.drug_class ,Estrogen receptor ,Cancer ,Articles ,Biology ,Cell cycle ,medicine.disease ,Oncology ,Estrogen ,medicine ,Immunohistochemistry ,Thymic carcinoma - Abstract
A number of previous studies have reported that sex steroid hormones, including estrogens, are involved in the regulation of the thymic function. The aim of the present study was to investigate the expression of estrogen receptor β5 (ERβ5) in thymic tumors and the correlation between ERβ5 expression and thymoma biological characteristics. The expression levels of ERβ5 in thymic epithelial tumors was evaluated in 103 patents using immunohistochemical staining and reverse transcription-quantitative polymerase chain reaction. In addition, an indirect immunofluorescence assay was performed to evaluate the ERβ5 expression levels in the TC1889 and T1682 cell lines. The survival outcome was estimated using Kaplan-Meier plots. The results indicated that ERβ5 expression was mainly located in the thymic tumor cell cytoplasm (87.37%; 90/103 cases) and overexpression was observed in thymic tumors compared with normal thymic tissues (P=0.001). Using the Kruskal-Wallis test, a statistically significant association was observed between cytoplasmic ERβ5 (cERβ5) expression and thymic tumor subtypes (P=0.024) and stages (P=0.003 and R=-0.376). The Kaplan-Meier plots revealed that cERβ5 expression was significantly associated with improved overall and progression-free survival (P=0.008 and P=0.004, respectively). The present study suggested that overexpression of cERβ5 may indicate an improved prognosis and may be involved in the underlying mechanism through which estrogen inhibits thymoma and thymic carcinoma development.
- Published
- 2015
9. Synthesis and Characterization of Mn(II) and Zn(II) Complexes with Tetrahydroxyl Substituted Salamo-type Compound
- Author
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Sheng-Ying Li, Zheng-Kun Wang, Gang Li, Meng-Meng Zhao, Li-Sha Zhang, Xin-Ying Zhang, and Jiao-Long Meng
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General Chemistry - Published
- 2013
10. Synthesis and Characterization of Co(II), Ni(II) and Cd(II) Complexes with Salamo-Type Compound Bearing Multihydroxyl Substituents
- Author
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Sheng-Ying Li, Zheng-Kun Wang, Meng-Meng Zhao, Xiu-Yan Dong, Jiao-Long Meng, Xin-Ying Zhang, and Li-Sha Zhang
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General Chemistry - Published
- 2013
11. [Effect of lactuside B on the expression of bcl-2 and bax mRNA and their protein in rats' cerebral cortex after cerebral ischemia-reperfusion injury]
- Author
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Sheng-ying, Li, Juan, Sun, Bing-xuan, Niu, Fu-lin, Yan, and He-qin, Zhan
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Cerebral Cortex ,Male ,Neurons ,Plants, Medicinal ,Dose-Response Relationship, Drug ,Vasodilator Agents ,Apoptosis ,Asteraceae ,Brain Ischemia ,Rats ,Rats, Sprague-Dawley ,Random Allocation ,Glucosides ,Proto-Oncogene Proteins c-bcl-2 ,Reperfusion Injury ,Animals ,RNA, Messenger ,Rhizome ,bcl-2-Associated X Protein - Abstract
This study is to investigate the effect of the major chemical composition in rhizome of Pterocypsela elata, lactuside B, on expression of bcl-2, bax mRNA and their protein in rats' cerebral cortex after cerebral ischemia-reperfusion injury. First, middle cerebral artery ischemia-reperfusion injury model was established, and each group was treated with the corresponding medicines. Animals were separately sacrificed at 24 h and 72 h. The brain infarct volumes were detected by TTC dye, bcl-2 and bax mRNA expression was checked by RT-PCR, and the proteins of bcl-2 and bax were explored by two-step immunohistochemistry in cerebral cortex of rats. Lactuside B can reduce brain infarct volume of cerebral cortex of rats, increase the expression of bcl-2 mRNA and decrease that of bax mRNA. Moreover, the ratio of bcl-2 to bax mRNA is higher in 12.5 and 25 mg kg(-1) dose group, respectively, which is significantly different from that of model group (P0.05 or P0.01). Generally, either 12.5 or 25 mg kg(-1) dose group is better than positive control medicine nimodipine (P0.05 or P0.01). In addition, the expression of bcl-2 and bax protein is consistent with their gene expression. Infarct volume and the ratio of bcl-2 to bax mRNA expression are significantly different (P0.05 or P0.01) between 72 h and 24 h group. The results demonstrated that lactuside B could play a good role in resisting cerebral ischemia by upregulating the expression of bcl-2 mRNA and protein and downregulating that of bax mRNA and protein.
- Published
- 2012
12. Evaluation on pore structures of organosilicate thin films by grazing incidence small-angle X-ray scattering
- Author
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Sheng-Ying Li, Detlef-M. Smilgies, Hong-Ji Chen, Xiao-jun Liu, Zhonghua Wu, Ruipeng Li, and Zhihong Li
- Subjects
Nanostructure ,Materials science ,Nanoporous ,Small-angle X-ray scattering ,Scattering ,Surface Properties ,Silicates ,X-Rays ,Membranes, Artificial ,Silsesquioxane ,Surfaces, Coatings and Films ,Nanostructures ,Crystallography ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Scattering, Small Angle ,Materials Chemistry ,Grazing-incidence small-angle scattering ,Physical and Theoretical Chemistry ,Thin film ,Porosity - Abstract
Nanoporous thin films fabricated by both a core-shell-shaped organic-inorganic hybrid sphere (octa(2,4-dinitrophenyl)silsesquioxane, ODNPSQ) and a four-leg-numbered surfactant (polyoxyethylene sorbitan monolaurate, Tween-20) for porogens in a higher molecular weight precursor (polyphenylsilsesquioxane, PPSQ) were characterized, respectively, by grazing incidence small-angle X-ray scattering (GISAXS), and the measured 2D GISAXS profiles were analyzed quantitatively by using a GISAXS formula based on the distorted wave Born approximation (DWBA). The fitted 2D GISAXS data show that the PPSQ porous thin films imprinted with ODNPSQ porogen exhibit sphere-shaped closed pores with the average pore size within a range of 1.18-3.12 nm and pore size distribution widths about 3.0 nm when the porogen loadings increase from 10 to 40 wt % and those imprinted with Tween-20 porogen give out an average pore size of 1.07-1.29 nm and pore size distribution widths about 2.0 nm with the porogen loading varying from 5 to 30 wt %. The nanoporous dielectric thin films imprinted with ODNPSQ porogen show a reducing to the molecular aggregation of porogens and significant antiphase separation behavior in the cross-linked matrix.
- Published
- 2009
13. Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
- Author
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Yi-Teng Xia, Edwin Hok-Chi Cheng, Ying-Jie Xia, Qi-Yun Wu, Lynn Hoi-Lam Zhang, Sheng-Ying Lin, Tina Ting-Xia Dong, Qi-Wei Qin, Wen-Xiong Wang, and Karl Wah-Keung Tsim
- Subjects
Cell line establishment ,Siganus fuscescens ,Anti-inflammation ,Traditional Chinese medicine ,Scutellaria baicalensis ,Zoology ,QL1-991 - Abstract
A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in different temperatures and serum concentrations: the best growing condition was optimized at 20% serum under 28 °C. In cultured RFM cells, sequencing of 18S rRNA, as well as immunostaining of cytokeratin and CD 68, confirmed the identity as macrophagic cell of S. fuscescens. Cultured RFM cells were exposed to challenge of inflammation, as triggered by LPS, showing highly sensitive responses to inflammation, including release of nitric oxide, expression of cytokine, and activation of phagocytosis. The water extract of aerial part of Scutellaria baicalensis, named as SBA, has been shown anti-inflammatory property in S. fuscescens fish. In order to extend the application of SBA in aquaculture, the extract and its effective flavonoids, i.e. baicalin and scutellarin, were applied in LPS-treated RFM cells. Application of SBA extract, baicalin or scutellarin, inhibited the expressions of LPS-induced inflammatory cytokines, i.e. IL-1β, TNF-α, as well as the signaling of transcription factor NF-κB. The results support the established RFM cell line could be an ideal in vitro model in drug screening relating to inflammation. Additionally, the notion of SBA herbal extract in fish aquaculture is supported by its efficacy against inflammation.
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- 2021
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14. Synthesis of a new aromatic dianhydride monomer and related polyimide
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Yun Xia Wei, Ming Guang Ma, Guo Hu Zhao, Ming Kai Chen, and Sheng Ying Li
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chemistry.chemical_compound ,Monomer ,chemistry ,Polymer chemistry ,Phenol ,General Chemistry ,Solubility ,Polyimide - Abstract
A novel aromatic dianhydride monomer, 3,3′-oxybis[(3,4-dicarboxyphenoxy)phenol] dianhydride, was successfully synthesized in three steps using 3,3′-oxybis(phenol) as starting material, which was reacted with 4,4′-oxydianiline (ODA) via a conventional thermal or chemical imidization method to produce a new polyimide. The resulting polyimide exhibited excellent solubility, and film-forming capability.
- Published
- 2009
15. Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression.
- Author
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SHENG-YING LI, YU-XIA WANG, LEI WANG, ZHI-BING QIAN, and MING-LI JI
- Subjects
- *
CYTOPLASM , *ESTROGEN receptors , *THYMOMA , *STEROID hormones , *TUMORS , *IMMUNOSTAINING - Abstract
A number of previous studies have reported that sex steroid hormones, including estrogens, are involved in the regulation of the thymic function. The aim of the present study was to investigate the expression of estrogen receptor β5 (ERβ5) in thymic tumors and the correlation between ERβ5 expression and thymoma biological characteristics. The expression levels of ERβ5 in thymic epithelial tumors was evaluated in 103 patents using immunohistochemical staining and reverse transcription-quantitative polymerase chain reaction. In addition, an indirect immunofluorescence assay was performed to evaluate the ERβ5 expression levels in the TC1889 and T1682 cell lines. The survival outcome was estimated using Kaplan-Meier plots. The results indicated that ERβ5 expression was mainly located in the thymic tumor cell cytoplasm (87.37%; 90/103 cases) and overexpression was observed in thymic tumors compared with normal thymic tissues (P=0.001). Using the Kruskal-Wallis test, a statistically significant association was observed between cytoplasmic ERβ5 (cERβ5) expression and thymic tumor subtypes (P=0.024) and stages (P=0.003 and R=-0.376). The Kaplan-Meier plots revealed that cERβ5 expression was significantly associated with improved overall and progression-free survival (P=0.008 and P=0.004, respectively). The present study suggested that overexpression of cERβ5 may indicate an improved prognosis and may be involved in the underlying mechanism through which estrogen inhibits thymoma and thymic carcinoma development. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
16. Lactuside B decreases aquaporin-4 and caspase-3 mRNA expression in the hippocampus and striatum following cerebral ischaemia-reperfusion injury in rats.
- Author
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PING-FA LI, HE-QIN ZHAN, SHENG-YING LI, RUI-LI LIU, FU-LIN YAN, TAI-ZHEN CUI, YU-PING YANG, PENG LI, and XIN-YAO WANG
- Subjects
LACTONES ,CEREBRAL ischemia treatment ,TREATMENT of reperfusion injuries ,CEREBRAL edema ,ANIMAL models in research ,THERAPEUTICS - Abstract
This study aimed to investigate the effects of lactuside B (LB) on aquaporin-4 (AQP4) and caspase-3 mRNA expression in the hippocampus and the striatum following cerebral ischaemia-reperfusion (I/R) injury in rats. Cerebral I/R injury was established in Sprague-Dawley rats by occluding the middle cerebral artery for 2 h and then inducing reperfusion. Rats in the I/R + LB groups were treated with various doses of LB following reperfusion. Neurological deficit scores and brain water content were obtained to determine the pharmacodynamics of LB. Reverse transcription polymerase chain reaction was performed to determine the expression levels of AQP4 and caspase-3 mRNA in the hippocampus and the striatum. The results of the present study indicate that LB decreased the neurological deficit scores and the brain water content. In the hippocampus, AQP4 and caspase-3 mRNA expression levels were significantly downregulated in the I/R + LB groups at 24 and 72 h following drug administration, compared with those in the I/R group (P<0.05). In the striatum, LB was also shown to significantly reduce AQP4 and caspase3 mRNA expression levels at 24 and 72 h following drug administration, compared with those in the I/R group (P<0.05). The effects became stronger as the LB dose was increased. The most significant reductions in AQP4 and caspase-3 mRNA expression were noted in the I/R + LB 25 mg/kg and I/R + LB 50 mg/kg groups at 72 h following drug administration. The results of the present study show that LB is capable of significantly downregulating AQP4 and caspase-3 mRNA expression in the hippocampus and striatum following cerebral I/R injury in rats. The mechanism by which LB improved ischaemic brain injury may be associated with changes in AQP4 and caspase-3 mRNA expression in the hippocampus and the striatum. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
17. Processing of ultrahigh molecular weight polyethylene
- Author
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Sheng-Ying Li, Xi-You Wang, and Ronald Salovey
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Materials science ,Polymers and Plastics ,Melting temperature ,Compression molding ,General Chemistry ,Calorimetry ,Recrystallization (chemistry) ,Surfaces, Coatings and Films ,Crystallization temperature ,Ultrahigh molecular weight polyethylene ,Crystallinity ,Chemical engineering ,Polymer chemistry ,Materials Chemistry ,Single crystal - Abstract
The extent of recrystallization of nascent UHMWPE powder is easily measured by calorimetry. Melting and recrystallization of nascent UHMWPE at 140°C can be suppressed by compression molding. Crystals of UHMWPE prepared from dilute solution show a peak melting temperature of 140°C and exhibit crystallinity up to 75.5% depending on crystallization temperature. Large changes in crystallinity result from drawing single crystal mats or compression-molded films.
- Published
- 1988
18. Identification and characterization of a novel hydroxylamine oxidase, DnfA, that catalyzes the oxidation of hydroxylamine to N2.
- Author
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Meng-Ru Wu, Li-Li Miao, Ying Liu, Xin-Xin Qian, Ting-Ting Hou, Guo-Min Ai, Lu Yu, Lan Ma, Xi-Yan Gao, Ya-Ling Qin, Hai-Zhen Zhu, Lei Du, Sheng-Ying Li, Chang-Lin Tian, De-Feng Li, Zhi-Pei Liu, and Shuang-Jiang Liu
- Subjects
- *
HYDROXYLAMINE , *ELECTRON paramagnetic resonance , *OXIDATION-reduction reaction , *NITROGEN , *ENZYME kinetics , *OXIDATION , *NITROGEN removal (Water purification) - Abstract
Nitrogen (N2) gas in the atmosphere is partially replenished by microbial denitrification of ammonia. Recent study has shown that Alcaligenes ammonioxydans oxidizes ammonia to dinitrogen via a process featuring the intermediate hydroxylamine, termed "Dirammox" (direct ammonia oxidation). However, the unique biochemistry of this process remains unknown. Here, we report an enzyme involved in Dirammox that catalyzes the conversion of hydroxylamine to N2. We tested previously annotated proteins involved in redox reactions, DnfA, DnfB, and DnfC, to determine their ability to catalyze the oxidation of ammonia or hydroxylamine. Our results showed that none of these proteins bound to ammonia or catalyzed its oxidation; however, we did find DnfA bound to hydroxylamine. Further experiments demonstrated that, in the presence of NADH and FAD, DnfA catalyzed the conversion of 15N-labeled hydroxylamine to 15N2. This conversion did not happen under oxygen (O2)-free conditions. Thus, we concluded that DnfA encodes a hydroxylamine oxidase. We demonstrate that DnfA is not homologous to any known hydroxylamine oxidoreductases and contains a diiron center, which was shown to be involved in catalysis via electron paramagnetic resonance experiments. Furthermore, enzyme kinetics of DnfA were assayed, revealing a Km of 92.9 ± 3.0 μM for hydroxylamine and a kcat of 0.028 ± 0.001 s-1. Finally, we show that DnfA was localized in the cytoplasm and periplasm as well as in tubular membrane invaginations in HO-1 cells. To the best of our knowledge, we conclude that DnfA is the first enzyme discovered that catalyzes oxidation of hydroxylamine to N2. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
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