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Myocardial ischemia/reperfusion (I/R) injury can stimulate mitochondrial reactive oxygen species production. Optic atrophy 1- (OPA1-) induced mitochondrial fusion is an endogenous antioxidative mechanism that preserves the mitochondrial function. In our study, we investigated whether melatonin augments OPA1-dependent mitochondrial fusion and thus maintains redox balance during myocardial I/R injury. In hypoxia/reoxygenation- (H/R-) treated H9C2 cardiomyocytes, melatonin treatment upregulated OPA1 mRNA and protein expression, thereby enhancing mitochondrial fusion. Melatonin also suppressed apoptosis in H/R-treated cardiomyocytes, as evidenced by increased cell viability, diminished caspase-3 activity, and reduced Troponin T secretion; however, silencing OPA1 abolished these effects. H/R treatment augmented mitochondrial ROS production and repressed antioxidative molecule levels, while melatonin reversed these changes in an OPA1-dependent manner. Melatonin also inhibited mitochondrial permeability transition pore opening and maintained the mitochondrial membrane potential, but OPA1 silencing prevented these outcomes. These results illustrate that melatonin administration alleviates cardiomyocyte I/R injury by activating OPA1-induced mitochondrial fusion and inhibiting mitochondrial oxidative stress.

With the establishment of China (Shanghai) Pilot Free Trade Zone (CSPFTZ). For remanufacture industry, Free Trade Zone (FTZ) has improved in some policies, such as import tariff, customs performance and timeliness of shipments. Regarding the remanufacturing network design considering FTZ as research subject, the maximum operating profit and the highest Logistics Performance Index (LPI) as objectives, an optimization model involving recovery ratio reflected by dynamic pricing will be build to study the collection strategies and specific logistics network layout of remanufacturing enterprise. Our conclusions show that, under the premise of just considering economic efficiency, the enterprise will still set up remanufacturing center outside the FTZ in spite of taking off the restriction of double-overseas industrial pattern. Meanwhile, along with the increase of the goal weight of LPI, lifting double-overseas industrial pattern restriction will cause the remanufacturing center transfer to FTZ and the bigger scale of the center.