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14 results on '"Shi, Wanrui"'

3. Lysine aggregates‐based nanostructured antimicrobial peptides for cariogenic biofilm microenvironment‐activated caries treatment.

Author

Li, Siyuan, Wang, Feng, Chen, Yang, Shi, Wanrui, Liu, Dashuai, Lv, Mingyang, Zhao, Bin, Liu, Yi, and Zhang, Hao

Subjects
ANTIMICROBIAL peptides, BACTERIAL cell surfaces, DENTAL caries, OXIDATION of glucose, REACTIVE oxygen species
Abstract

Dental caries is one of the most prevalent and costly biofilm‐induced oral diseases that causes the deterioration of the mineralized tooth tissue. Traditional antimicrobial agents like antibiotics and antimicrobial peptides (AMPs) struggle to effectively eradicate bacteria in biofilms without eliciting resistance. Herein, we demonstrate the construction of FeOOH@Fe‐Lysine@Au nanostructured AMPs (nAMPs) distinguished by their AMP‐like antibacterial activity and self‐producing reactive oxygen species (ROS) capacity for caries treatment. On the one hand, FeOOH@Fe‐Lysine@Au nAMPs can catalyze glucose oxidation to generate ROS within the cariogenic biofilm microenvironment, resulting in the disintegration of the extracellular polymeric substance matrix and the exposure of bacteria. On the other hand, FeOOH@Fe‐Lysine@Au nAMPs can attach to bacterial surfaces via electrostatic attractions, proceeding to damage membranes, disrupt metabolic pathways, and inhibit protein synthesis through the aggregated lysine and the generated ROS. Based on this antibacterial mechanism, FeOOH@Fe‐Lysine@Au nAMPs can effectively eradicate Streptococcus mutans and its associated biofilm, significantly impeding the progression of dental caries. Given the straightforward and cost‐efficient preparation of FeOOH@Fe‐Lysine@Au nAMPs compared to AMPs that require specific sequences, and their minimal adverse impacts on gingival/palatal tissues, major organs, and oral/gut microbiomes, our research may promote the development of novel therapeutic agents in dental health maintenance. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Ferroptosis and Necroptosis Produced Autologous Tumor Cell Lysates Co-Delivering with Combined Immnoadjuvants as Personalized In SituNanovaccines for Antitumor Immunity

Author

Shi, Wanrui, Feng, Wenjie, Li, Siyuan, Cui, Yanqi, Liu, Shuwei, Jiang, Huan, Liu, Yi, and Zhang, Hao

Abstract

Nanovaccine-based immunotherapy has been considered as a major pillar to stimulate the host immune system to recognize and eradicate tumor cells as well as establish a long-term immune memory to prevent tumor relapse and metastasis. However, the weak specificity and low cross-presentation of antigens, as well as the immunosuppressive microenvironments of tumor tissues, are still the major obstacles on exerting the therapeutic performance of tumor nanovaccines sufficiently. Herein, we design and construct cytosine guanine dinucleotide (CpG) oligodeoxynucleotide (ODN)-loaded aluminum hydroxyphosphate nanoparticles covered by Fe-Shikonin metal-phenolic networks (MPNs) (Alum-CpG@Fe-Shikonin NPs) as personalized in situnanovaccines for antitumor immunity. Upon internalization by tumor cells, the shell of Fe-Shikonin MPNs will disassemble into Fe2+and Shikonin to elicit the immunogenic cell death of tumor cells through ferroptosis and necroptosis. Then, dying tumor cell-released autologous tumor cell lysates will be absorbed by Alum NPs and codelivered with CpG ODN to professional antigen-presenting cells temporally and spatially to activate multistep cascade antitumor immune responses, including dendritic cell maturation, antigen cross-presentation, natural killer cell and cytotoxic T lymphocyte infiltrations, and tumor-associated macrophage repolarization. Benefiting from the synergistic effects of Alum NPs, CpG ODN, and Fe-Shikonin MPNs, our Alum-CpG@Fe-Shikonin NPs exhibit drastic cytotoxicity and accurate selectivity on eradicating primary tumor, strong abscopal effect on inhibiting distant tumor, and a long-term immune memory effect on preventing tumor metastasis and recurrence. Because our report provides a feasible strategy to in situmake full use of autologous tumor cell lysates, which present an entire spectrum of the patient’s personal epitopes without complicated ex vivoprocesses, such as extraction, purification, and sequencing, it may promote the development of personalized nanovaccines for antitumor immunity.

Published
2023
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11. Back Cover: Lysine aggregates‐based nanostructured antimicrobial peptides for cariogenic biofilm microenvironment‐activated caries treatment.

Author

Li, Siyuan, Wang, Feng, Chen, Yang, Shi, Wanrui, Liu, Dashuai, Lv, Mingyang, Zhao, Bin, Liu, Yi, and Zhang, Hao

Subjects
GOLD nanoparticles, ANTIMICROBIAL peptides, BACTERIAL cell walls, REACTIVE oxygen species, PROTEIN synthesis
Abstract

Aggregated lysine mimics antimicrobial peptides (AMPs) by disrupting bacterial membranes, interfering with metabolism, and inhibiting protein synthesis. With Fe3+ hydrolysis, lysine coordination, and ultrasmall Au nanoparticle deposition, FeOOH@Fe‐Lysine@Au shows AMP‐like antibacterial activity and reactive oxygen species generation, providing an effective approach for cariogenic biofilm microenvironment‐activated caries treatment (e578)..By Siyuan Li; Feng Wang; Yang Chen; Wanrui Shi; Dashuai Liu; Mingyang Lv; Bin Zhao; Yi Liu and Hao ZhangReported by Author; Author; Author; Author; Author; Author; Author; Author; Author [Extracted from the article]

Published
2024
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13. Fe(III)‐Shikonin Supramolecular Nanomedicine for Combined Therapy of Tumor via Ferroptosis and Necroptosis

Author

Hao Zhang, Pengfei Ge, Wenjie Feng, Shuwei Liu, Shi Wanrui, Yi Liu, and Huiwen Liu

Subjects
Chemotherapy, Programmed cell death, biology, Necroptosis, medicine.medical_treatment, Integrin, Biomedical Engineering, Supramolecular chemistry, Pharmaceutical Science, Glutathione, Ferric Compounds, Biomaterials, chemistry.chemical_compound, Nanomedicine, chemistry, In vivo, Cell Line, Tumor, biology.protein, Cancer research, medicine, Ferroptosis, Humans, Naphthoquinones
Abstract

Most of the antitumor chemotherapeutic drugs execute the therapeutic performance upon eliciting tumor cell apoptosis, which may cause chemoresistance of tumors. Design of novel drugs to eradicate apoptosis-resistant tumors via non-apoptotic cell death pathways is promising for improving the long-term chemotherapeutic efficacy. Herein, a Fe(III)-Shikonin metal-polyphenol-coordinated supramolecular nanomedicine for combined therapy of tumor via ferroptosis and necroptosis is designed. The construction of the nanomedicine based on the coordinated self-assembly between Fe3+ and Shikonin not only overcomes the shortcomings of Shikonin including its low bioavailability and high toxicity toward normal tissues, but also integrates the theranostics functions of Fe ions. Under the exposure of the high concentration of glutathione (GSH) in tumor cells, the as-prepared nanomedicine will disassemble into Fe2+ and Shikonin, followed by stimulating the tumor cell death through ferroptosis and necroptosis. In addition, benefiting from the stealth effect of polyethylene glycol (PEG) and the targeting ability of cyclo(Arg-Gly-Asp-d-Phe-Lys) (cRGD) to αv β3 -integrin, NH2 -PEG-cRGD-modified nanomedicine exhibits a GSH-responsive therapy toward 4T1 tumor in vivo and self-enhanced longitudinal relaxation (T1 )-weighted imaging property. Since the self-assembly of natural Shikonin and human body-necessary Fe element is facile and feasible, the work may provide a promising supramolecular nanomedicine for next-generation chemotherapeutic applications.

Published
2021

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