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Objective: Due to the incidence of resistance, a series of sulfonamide-derived 1,2,4- triazoles were synthesized and evaluated. Method: The novel sulfonamide-derived 1,2,4-triazoles were prepared starting from commercial acetaniline and chlorosulfonic acid by sulfonylation, aminolysis, N-alkylation and so on. The antimicrobial activity of the synthesized compounds were evaluated in vitro by two-fold serial dilution technique. Results: In vitro antimicrobial evaluation found that 2-chlorobenzyl sulfonamide 1,2,4-triazole 7c exhibited excellent antibacterial activities against MRSA, B. subtilis, B. typhi and E. coli with MIC values of 0.02−0.16 μmol/mL, which were comparable or even better than Chloromycin. The preliminary mechanism suggested that compound 7c could effectively bind with DNA, and also it could bind with human microsomal heme through hydrogen bonds in molecular docking. Computational chemical studies were performed on compound 7c to understand the structural features that are essential for activity. Additionally, compound 7c could generate a small amount of reactive oxygen species (ROS). Conclusion: Compound 7c could serve as a potential clinical antimicrobial candidate.

A novel series of benzimidazole-incorporated sulfonamide analogues were designed and synthesized with an effort to overcome the increasing antibiotic resistance. Compound 5c gave potent activities against Gram-positive bacteria and fungi, and 2,4-dichlorobenzyl derivative 5g showed good activities against Gram-negative bacteria. Both of these two active molecules 5c and 5g could effectively intercalate into calf thymus DNA to form compound-DNA complex respectively, which might block DNA replication to exert their powerful antimicrobial activity. Molecular docking experiments suggested that compounds 5c and 5g could insert into base-pairs of DNA hexamer duplex by the formation of hydrogen bonds with guanine of DNA. The transportation behavior of these highly active compounds by human serum albumin (HSA) demonstrated that the electrostatic interactions played major roles in the strong association of active compounds with HSA, and which was also confirmed by the full geometry calculation optimizations.

Objective Starting from the social scene under the background of mobile Internet, this paper explores a new information input and output scheme by connecting the human-computer interaction accompanied by the development of science and technology with the emotional needs of users. Methods Firstly, qualitative analysis and quantitative verification were conducted on the problems encountered by users using existing chat tools through user interviews and questionnaires. Then the interface design and interaction design were carried out based on ViP product design principles. Finally, interview, questionnaire and eye movement test were used to further improve the design scheme. Conclusion With the further development and improvement of science and technology, more diversified, efficient and interesting ways of information interaction will become possible.

At present，for the application of the fuzzy PID controller is analyzed in electronic load，this paper according to the shortcomings of the controller improved fuzzy neural PID controller, through the BP neural network to realize fuzzy function, the results shows that this method is feasible and efficient. Finally the method was simulated on MATLAB, the control effect of overshoot and time delay on the application of improved fuzzy neural PID control was confirmed better than the former methods.