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Combinational therapy such as taking tranexamic acid while using laser treatment has been proved potential efficacy by many experiments. However, there is few research which contains large samples and consistent observations.We evaluated clinical efficacy and safety of a new systemic treatment of drug-laser-photon therapy.Retrospective and randomized investigator-blinded study of 75 patients with mixed type melasma was analyzed. At each visit, standardized photographs were taken using VISIA. Modified melasma area and severity index (mMASI) scores were marked using photographs by two dermatologists.The mMASI score decreased significantly from 6.92 to 3.84 after the treatment. The VISIA analyze right cheek data shows: Spots (from 49.67 ± 3.43 to 56.09 ± 3.31), UV spots (from 41.39 ± 24.45 to 44.56 ± 25.86), and Brown spots (from 23.97 ± 17.89 to 28.16 ± 21.28) are statistically increased (p = 0.035, p = 0.018, p = 0.07). All patients feel varying degrees of improvement, about 10.17% felt very much improved, 30.51% felt much improved (51%-75%), 45.76% felt moderately improved (26%-50%), and 13.56% felt little improved (1%-25%).This study was no control group.The efficacy and safety profile of the combination of drug-laser-photon therapy systemic treatment in melasma patients has been proved. It has potential possibility to become a new, reliable, widely suitable therapy strategy.

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Tanghe wastewater reservoir(TWR) is located on the west side of Baiyangdian Lake in Xiong'an New Area, where sewage infiltration and irrigation has been taking place for 40 years, and a large number of contaminants have accumulated in the unsaturated zone. Identifying the mechanisms by which this combined system contributes to groundwater hydrochemical dynamics is important for the protection of the water environment in the area. Hydrogeochemical methods such as tracing and improved chlor-alkali index are used to analyze the spatial and temporal characteristics and evolution mechanisms of shallow groundwater. The study shows that the groundwater chemistry in the sewage reservoir area is SO

The variations of hydrogen and oxygen isotopes in rainfall are critical for understanding the sources of rainfall and the influence of local evaporation. Satellite precipitation products with high time resolution (for instance 1 h) could be helpful for testifying the accuracy of water sources, as it can clearly illustrate the route of cloud movement. In this study, we analyzed the composition of hydrogen and oxygen isotopes in different rainfall events in three stations from 2015 to 2018 along the transection of 38° N latitude from Taihang Mountains to the coastal region in North China, Taihang Mountain Station (mountainous area), Luancheng Station (pre-mountain plain) and Nanpi Station (coastal low plain). By selecting typical rainfall events, water vapor sources and its influence rainfall on hydrogen and oxygen isotopes were analyzed with hourly available CMORPH satellite precipitation products. Results showed that the hydrogen and oxygen isotopes of precipitation were cha-racterized by enrichment in the rainy season and depletion in the dry season. The hydrogen and oxygen isotopes in the rainy season showed a tendency of depletion with the increases of precipitation. The slope and intercept of the fitted relationship of hydrogen and oxygen isotopes in the piedmont region of the mountains were the lowest, indicating that precipitation in the piedmont plain was significantly affected by secondary evaporation fractionation. The effect of evaporation resulted in the largest variations of isotope ratio in the dry year. In the mountainous station, due to the heavy rainfall, large isotopic variation was found in rich precipitation year. Based on the route analysis of sate-llite precipitation products, dominant water vapor in the region was inland and northwest-oriented water vapor, while water vapor in the rainy season was from southwest and from the Pacific Ocean. There was a significant difference in the hydrogen and oxygen isotopes of precipitation in the mountainous and plain stations in 2016, owing to water vapor sources and effects of rainfall for the mountainous and evaporation for plain. The results from HYSPLIT model showed that during the rainstorm on 19th July in 2016, water vapor at the mountainous station was mainly from the southwest, while that in the coastal plain was a mixture of southwest and southeast sources. Overall, our results showed that spatial and temporal variations of hydrogen and oxygen isotopes were controlled by both water sources and evaporation processes along the transection of 38° north latitude in North China.降水氢氧同位素的变化程度对反演降水水汽来源和认识蒸发作用的强弱有重要作用;结合高时间分辨率的卫星降水产品能够提高反演水汽来源的准确性,更清晰地说明水汽团的运移路径。本研究以位于华北北纬38°带的中国科学院太行山站(山区)、栾城站(山前平原)和南皮站(滨海低平原)2015—2018年降水氢氧同位素为对象,分析该区域降水水汽来源和极端降水对氢氧同位素的影响。结果表明: 华北北纬38°带降水氢氧同位素在年内呈雨季富集、旱季贫化的特征,雨季降水氢氧同位素表现出随降水量增加而贫化的趋势;华北山前平原大气降水氢氧同位素拟合关系斜率和截距最小,说明山前平原降水受二次蒸发作用的影响最显著,蒸发作用也使得平原区枯水年同位素变幅最大,而山区雨量效应造成丰水年同位素变幅最大。利用卫星产品揭示降水的水汽来源,华北北纬38°带全年主导水汽为西北和内陆方向水汽,而雨季为西南和近缘海洋性水汽。由于水汽来源的变化,同时,山区和平原区分别受雨量效应和二次蒸发的影响,使得降水氢氧同位素在丰水年(2016)存在显著差异。用HYSPLIT模型模拟2016年“7·19”极端降水的路径,西南水汽是山区和平原区极端降水的主要水汽来源,而低平原区混合了东南向水汽。说明水汽源的差异及蒸发过程主导华北北纬38°带极端降水氢氧同位素的时空分布特征。.

Previous research has revealed that miR-215 might be an important miRNA regulating weaned piglets’ resistance to Escherichia coli (E. coli) F18. In this study, target genes of miR-215 were identified by RNA-seq, bioinformatics analysis and dual luciferase detection. The relationship between target genes and E. coli infection was explored by RNAi technology, combined with E. coli stimulation and enzyme linked immunosorbent assay (ELISA) detection. Molecular regulating mechanisms of target genes expression were analyzed by methylation detection of promoter regions and dual luciferase activity assay of single nucleotide polymorphisms (SNPs) in core promoter regions. The results showed that miR-215 could target EREG, NIPAL1 and PTPRU genes. Expression levels of three genes in porcine intestinal epithelial cells (IPEC-J2) in the RNAi group were significantly lower than those in the negative control pGMLV vector (pGMLV-NC) group after E. coli F18 stimulation, while cytokines levels of TNF-α and IL-1β in the RNAi group were significantly higher than in the pGMLV-NC group. Variant sites in the promoter region of three genes could affect their promoter activities. These results suggested that miR-215 could regulate weaned piglets’ resistance to E. coli F18 by targeting EREG, NIPAL1 and PTPRU genes. This study is the first to annotate new biological functions of EREG, NIPAL1 and PTPRU genes in pigs, and provides a new experimental basis and reference for the research of piglets disease-resistance breeding.

Two undescribed triterpenes, tricholimbrins A and B, three undescribed steroids, tricholimbrins C‒E, one undescribed 4-chromanone derivative, along with 27 known compounds were isolated from fruiting bodies of the mushroom Tricholoma imbricatum. Tricholimbrins A and B are two polycyclic triterpenoids with a carbon degradation, while tricholimbrin C is a ring-rearranged steroid containing an aromatic moiety that might be derived from an ergosterol. Isocyathisterol, 3β,15α-dihydroxyl-(22E,24R)-ergosta-5,8(14),22-trien-7-one, demethylincisterol A3, and volemolide showed cytotoxicities to six human cancer cell lines. 3β-Hydroxyl-(22E,24R)-ergosta-5,8,22-trien-7,15-dione and 3β-hydroxyl-(22E,24R)-ergosta-5,8,22-trien-7-one showed preferable cytotoxicities against HL-60 while chaxine C and volemolide showed preferable cytotoxicities against A-549, with IC50 values less than 10 μM.

Sjögren's syndrome (SS) is a systemic autoimmune disease that is characterized by focal lymphocytic infiltration into exocrine organs such as salivary and lacrimal glands, resulting in dry mouth and eyes, and other systemic injuries. There is no curative clinical therapy for SS, and stem cell therapy has shown great potential in this area. The mesenchymal stem cells (MSCs) in the salivary glands of healthy individuals and in patients with SS have not been extensively studied. The aim of this study was to elucidate the characteristics of MSCs from the labial glands of healthy controls and of those from patients with SS to elucidate the related pathogenesis and to uncover potential avenues for novel clinical interventions. Labial glands from patients with SS and healthy subjects were obtained, and MSCs were isolated and cultured by using the tissue adherent method. The MSC characteristics of the cultured cells were confirmed by using morphology, proliferation, colony forming-unit (CFU) efficiency, and multipotentiality, including osteogenic, adipogenic, and salivary gland differentiation. The MSCs from the healthy controls and SS patients expressed characteristic MSC markers, including CD29, CD44, CD73, CD90, and CD105; they were negative for CD34, CD45, and CD106, and also negative for the salivary gland epithelium markers (CD49f and CD117). Labial gland MSCs from both groups were capable of osteogenic and adipogenic differentiation. The CFU efficiency and adipogenic differentiation potential of MSCs were significantly lower in the SS group compared with the healthy controls. Cells from both groups could also be induced into salivary gland-like cells. Real-time polymerase chain reaction and immunofluorescence staining showed that the gene and protein expression of AMY1, AQP5, and ZO-1 in cells from the SS group was lower than that in cells from the healthy group. Thus, MSCs from the labial glands in patients with SS could lack certain characteristics and functions, especially related to salivary secretion. These preliminary data provided insights that could lead to the development of novel therapeutic strategies for the treatment of SS.

Taihang Mountain region is the recharge area for groundwater in the North China Plain (NCP). In recent years, the elevated nitrate concentration in the groundwater in the Taihang Mountain has often been associated with the increased area of farmland and the excessive application of nitrogen. Thus, it is significant to study the soil nitrogen leaching process in typical farmland. In this study, the root zone water quality model (RZWQM) was used to simulate the nitrate nitrogen leaching of winter wheat/summer maize rotation systems in the Hilly Ecosystem Experimental Station in Taihang Mountain. The results showed that during the 2015-2016 winter wheat/summer maize season, the nitrate nitrogen from the soil leaching occurred mainly in the summer maize season (rainy season), with the nitrate nitrogen leaching amount reaching 59.9 kg·hm

In order to study the source of nitrate in surface water and groundwater near a domestic sewage discharge river in the Baiyangdian watershed, including the Fuhe River and Baiyangdian Lake, shallow groundwater and deep groundwater samples were collected in July 2014.Water chemistry and stable isotopes (δ

We explored the applicability of Facescan three-dimensional (3D) facial reconstruction technology for adjunctive diagnosis and therapeutic evaluation of cheilitis granulomatosa (CG) in 33 patients with CG and 29 healthy controls at the Dept. of Oral Medicine, Peking University, School and Hospital of Stomatology (PKUSS), from January 2015 to May 2016. The Facescan structured-light 3D facial reconstruction scanner was used to scan the scope of lips in both groups, in order to acquire 3D morphological data of the lips. The lengths of six characteristic line segments were measured from the 3D lip model of the two groups, and the acquired data were compared. The results showed that the distance between the labiale superius and labiale inferius, and the lengths of the upper and lower vermilion borders showed significant differences between the CG and control groups, by using the 3D lip model. Thus, Facescan 3D facial reconstruction technology showed good reproducibility in the evaluation of lip swelling in CG patients, and it can be used to analyse the degree of lip swelling and evaluate the therapeutic efficacy of different treatments for CG.

(Gd,La)2-x O2CO3:Eux3+(0.01 ≤x≤0.04) were synthesized via a flux method at 400°C, and their photoluminescence properties under vacuum ultraviolet (VUV) excitation were examined. The excitation spectra showed two bands in the region from 125 nm to 300 nm, the first band centered at 190 nm was ascribed to absorption of related CO32- complex, and the second broad band centered at 246nm and 278nm in Gd2O2CO3:Eu3+ was ascribed to the charge transfer band of O2-→ Eu3+. Series samples exhibited red emission at around 611 nm under vacuum ultraviolet excitation corresponding to the 5D0→ 7F2.transition of Eu3+.

The photoaging of coating based on toluene diisocyanate (TDI) and N75 was investigated though artificial light exposure. The properties of studied polyurethane coatings, such as film gloss loss and discoloration were examined at some aging interval. In addition, scanning electronic microscopy (SEM) and Fourier transform infra-red spectroscopy (FTIR) were used to measure the morphology and chemical change of coatings before and after exposed under UV light. It was found that the addition of N75 had a significant effect on the discoloration of polyurethane coating. Furthermore, SEM images showed that greater degradation occurred in the coating without N75. These findings were also supported by the results obtained from FTIR analysis and gloss loss. The above results suggested that a mixed curing agent system with a mass ratio of TDI to N75 between 5:5 and 3:7 be a possible substitute of pure N75 for weather resistance PU coatings.

A protocol for successful callus induction and plant regeneration from Primula forbesii Franch. anthers is described. We first examined morphological characteristics of flower buds at different microspore developmental stages, then utilized anthers at the appropriate developmental stage for callus induction. Cultures were initiated on Murashige and Skoog (MS) medium supplemented with 1.0–2.0 mg/l 6-benzylaminopurine (BAP) and 0.5–2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The highest callus induction ratio (2.4%) was produced on MS + 1.0 mg/l BAP + 0.5 mg/l 2,4-D. Nine regeneration media were tested for callus shoot regeneration, with BAP from 0.1 to 2.0 mg/l and α-naphthaleneacetic acid (NAA) from 0.01 to 0.5 mg/l. The highest induction and proliferation of indefinite buds (55.2%) was produced on MS + 0.2 mg/l BAP + 0.01 mg/l NAA. Plant regulator free MS was also found to be the best rooting medium. Among a total of 516 anther-derived plantlets flow cytometry and cytological analysis identified 2% to be haploid, 65% diploid, 9% triploid, 5% tetraploid, 2% hexaploid, and 17% mixoploid. This protocol provides a useful foundation for further research toward the development of homozygous P. forbesii or other Primula species.

To investigate the corrosion state of subsea pipeline under cathodic protection, the surface potential distribution of the pipeline has to be measured exactly, but it’s very difficult to measure directly in actually. On base of boundary element methods (BEM), a 2D model about pipe’s cathodic protection under sea water was established, and then software to calculate the electric field distribution on pipe surface in even media (seawater) was developed by way of Matlab. The developed software was verified through an experiment in lab. The result shows that numerical calculation based on BEM by Matlab is feasible for cathodic protection electric field of pipe in marine environment.

There is an obvious regional contradiction between water resources and agricultural produce in lower plain area of North China, however, excessive fluorine in deep groundwater further limits the use of regional water resources. In order to understand the spatial distribution characteristics and source of F(-) in groundwater, study was carried out in Nanpi County by field survey and sampling, hydrogeochemical analysis and stable isotopes methods. The results showed that the center of low fluoride concentrations of shallow groundwater was located around reservoir of Dalang Lake, and centers of high fluoride concentrations were located in southeast and southwest of the study area. The region with high fluoride concentration was consistent with the over-exploitation region of deep groundwater. Point source pollution of subsurface drainage and non-point source of irrigation with deep groundwater in some regions were the main causes for the increasing F(-) concentrations of shallow groundwater in parts of the sampling sites. Rock deposition and hydrogeology conditions were the main causes for the high F(-) concentrations (1.00 mg x L(-1), threshold of drinking water quality standard in China) in deep groundwater. F(-) released from clay minerals into the water increased the F(-) concentrations in deep groundwater because of over-exploitation. With the increasing exploitation and utilization of brackish shallow groundwater and the compressing and restricting of deep groundwater exploitation, the water environment in the middle and east lower plain area of North China will undergo significant change, and it is important to identify the distribution and source of F(-) in surface water and groundwater for reasonable development and use of water resources in future.

Background Invasive aspergillosis (IA) is an important cause of mortality in critically ill patients, but the diagnosis is difficult as clinical and radiological signs are neither sensitive nor specific. Serum galactomannan (GM) is a useful marker for IA, but exhibits low sensitivity in non-neutropenic patients. In our previous work, strong antibody reactivity to thioredoxin reductase of Aspergillus fumigatus was found in non-neutropenic IA patients. Methods and results Using recombinant thioredoxin reductase GliT (TR), an antigenic protein secreted by A. fumigatus , as the coating antigen, an enzyme-linked immunosorbent assay (ELISA) for detecting anti-TR antibodies was developed. The antibody response to TR in IA animal models and 42 non-neutropenic patients with culture- and/or histology-documented IA was investigated. The results showed that anti-TR antibody was detectable in rabbit serum 7–9 days after exposure to the fungus. The sensitivity and specificity of the anti-TR antibody assay in patients were 80.9% and 96%, respectively, while the sensitivity of GM in this group of patients was only 52.3%. The specificity of the assay was confirmed by testing the sera from patients infected with other pathogenic fungal species and bacteria.

This report uses the intermediate disturbance hypothesis to assess the influence of constructing a check dam on river environment. HEC-HMS and HEC-RAS programs were used to generate hydraulic parameters such as flow discharge, water depth, velocity, water surface width and sediment discharge. Indicators of hydrologic alteration (IHA) and indicator of habitat alteration (IHabA) were used to evaluate the flow and habitat conditions before and after check dam construction. The range of variability approach was used to calculate the degree of hydrologic alteration for each IHA, degree of habitat alteration and overall alteration for IHabA. Variability of river habitats before and after check dam construction was contrasted. Alteration became larger the closer to the dam body. An assessment method for check dam construction is offered which does not require ecological investigation data and combines ecology concepts and hydraulics.

To explore the electroencephalogram (EEG) changes after occlusal trauma in rats.Health Wistar rats were divided into 2 groups: traumatic occlusion group (1-day, 3-day, 7-day, 14-day, 28-day group) and control group. The electrodes were inserted in the hippocampus of anesthetized rats guided by stereotaxic apparatus of brain and then the hippocampal EEG were recorded during sleep. Data were analysed by SPSS 17.0 software package for ANOVA.The δ waves were increased and the frequency and amplitude of EEG were significantly reduced in 1-day traumatic group(P0.05); The waves were decreased and the frequency and amplitude of EEG were significantly increased in 3-day traumatic group(P0.05). There was no significant difference compared with the control group. The δ waves were decreased in 7-day and 14-day traumatic group and the frequency and amplitude of EEG were significantly increased (P0.05). The indexes in the 28-day traumatic group returned to normal.Occlusal trauma can change the EEG of the rat during sleep. It mainly affects the deep sleep, and reduces sleep quality. Supported by Innovation Fund of Shandong University (2010GN048).

Background There has been a rising incidence of invasive aspergillosis (IA) in critically ill patients, even in the absence of an apparent predisposing immunodeficiency. The diagnosis of IA is difficult because clinical signs are not sensitive and specific, and serum galactomannan has relatively low sensitivity in this group of patients. Therefore, more prompt and accurate disease markers for early diagnosis are needed. To establish disease markers demands a thorough knowledge of fungal antigens which may be detected in the serum or other body fluids of patients. Herein we report novel immunodominant antigens identified from extracellular proteins of Aspergillus fumigatus. Results Extracellular proteins of A. fumigatus were separated by two-dimensional electrophoresis (2-DE) and probed with the sera from critically ill patients with proven IA. The immunoreactive protein spots were identified by MALDI-TOF mass spectrometry (MALDI-TOF -MS). Forty spots from 2DE gels were detected and 17 different proteins were identified as immunogenic in humans. Function annotation revealed that most of these proteins were metabolic enzymes involved in carbohydrate, fatty acid, amino acid, and energy metabolism. One of the proteins, thioredoxin reductase GliT (TR), which showed the best immunoactivity, was analyzed further for secretory signals, protein localization, and homology. The results indicated that TR is a secretory protein with a signal sequence exhibiting a high probability for secretion. Furthermore, TR did not match any human proteins, and had low homology with most other fungi. The recombinant TR was recognized by the sera of all proven IA patients with different underlying diseases in this study. Conclusions The immunoreactive proteins identified in this study may be helpful for the diagnosis of IA in critically ill patients. Our results indicate that TR and other immunodominant antigens have potential as biomarkers for the serologic diagnosis of invasive aspergillosis.

The behaviour, failure types, torsional strength and the relationship between shear and torsional strength, have been studied in this paper by means of experiments on 28 reinforced keramzite concrete members. Based on these experiments and theoretical analysis, practical methods to calculate the strength of members under combined bending, shear and torsion are advanced.