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253 results on '"Shiloni, Eitan"'

26. Interleukin-6 secretion in mice is associated with reduced glucose-6-phosphatase and liver glycogen levels

Author

Metzger, Shula, Goldschmiit, Neta, Barash, Varda, Peretz, Tamar, Drize, Olga, Shilyansky, Joel, Shiloni, Eitan, and Chajek-Shaul, Tova

Subjects
Interleukin-6 -- Research, Mice -- Research, Glucose-6-phosphate dehydrogenase deficiency -- Research, Liver -- Research, Biological sciences
Abstract

Research was undertaken to study the chronic effect of interleukin-6 (IL-6) on carbohydrate metabolism using mice bearing IL-6-secreting tumor. Mice were injected with allogeneic tumor cells transduced with the murine IL-6 gene. Secretion of IL-6 from the developed tumors was associated with decreased food consumption, reduced body weight and reduced blood glucose levels. Insulin levels did not change and 2-deoxyglucose uptake was not affected in most tissues examined.

Published
1997

34. Pancreaticojejunostomy versus controlled pancreaticocutaneous fistula in pancreaticoduodenectomy for periampullary carcinoma

Author

Reissman, Petachia, Perry, Yaron, Cuenca, Abigo, Bloom, Allan, Eid, Ahmed, Shiloni, Eitan, Rivkind, Abraham, and Durst, Arieh

Subjects
Pancreatectomy -- Complications, Gastrointestinal cancer, Surgery -- Methods, Health
Abstract

BACKGROUND: Anastomotic leak of the pancreaticojejunostomy is a major cause of morbidity and mortality following pancreaticoduodenectomy. Reports have described a large variety of techniques for performing this anastomosis and managing the pancreatic stump. In an attempt to obviate the pancreaticojejunostomy, we prospectively studied the technique of ligating the pancreatic duct and using external drains to create a temporary controlled pancreaticocutaneous fistula. PATIENTS AND METHODS: Thirty-five consecutive patients who were to undergo pancreaticoduodenectomy for periampullary carcinoma were prospectively randomized to one of two groups: pancreaticojejunostomy (PJ) (n = 18) or controlled pancreaticocutaneous fistula (CPF) (n 17). The groups were well matched for age, sex, coexisting medical illnesses, type of tumor, and preoperative condition. Except for the management of the pancreatic remnant, all patients in both groups underwent an identical procedure. Major morbidity, length of hospitalization, duration of the controlled pancreatic fistula, and mortality were analyzed over a mean follow-up interval of 26 months (range 5 months to 7.5 years). RESULTS: The CPF group experienced lower overall operative morbidity rates than the PJ group (24% versus 56%, P

Published
1995

37. Adjuvant Autologous Melanoma Vaccine For Macroscopic Stage Iii Disease: Survival, Biomarkers, And Improved Response To Ctla-4 Blockade

Author

Lotem, Michal, Merims, Sharon, Frank, Stephen, Hamburger, Tamar, Nissan, Aviram, Kadouri, Luna, Cohen, Jonathan, Straussman, Ravid, Eisenberg, Galit, Frankenburg, Shoshana, Carmon, Einat, Alaiyan, Bilal, Shneibaum, Shlomo, Ayyildiz, Zeynep Ozge, Isbilen, Murat, Senses, Kerem Mert, Ron, Ilan, Steinberg, Hanna, Smith, Yoav, and Shiloni, Eitan

Abstract

Background. There is not yet an agreed adjuvant treatment for melanoma patients with American Joint Committee on Cancer stages III B and C. We report administration of an autologous melanoma vaccine to prevent disease recurrence. Patients and Methods. 126 patients received eight doses of irradiated autologous melanoma cells conjugated to dinitrophenyl and mixed with BCG. Delayed type hypersensitivity (DTH) response to unmodified melanoma cells was determined on the vaccine days 5 and 8. Gene expression analysis was performed on 35 tumors from patients with good or poor survival. Results. Median overall survival was 88 months with a 5-year survival of 54%. Patients attaining a strong DTH response had a significantly better (p = 0.0001) 5-year overall survival of 75% compared with 44% in patients without a strong response. Gene expression array linked a 50-gene signature to prognosis, including a cluster of four cancer testis antigens: CTAG2 (NY-ESO-2), MAGEA1, SSX1, and SSX4. Thirty-five patients, who received an autologous vaccine, followed by ipilimumab for progressive disease, had a significantly improved 3-year survival of 46% compared with 19% in nonvaccinated patients treated with ipilimumab alone (p = 0.007). Conclusion. Improved survival in patients attaining a strong DTH and increased response rate with subsequent ipilimumab suggests that the autologous vaccine confers protective immunity.

Published
2016

41. Metachronous Colon Metastasis to the Thyroid: A Case Report and Literature Review

Author

Froylich, Dvir, Shiloni, Eitan, and Hazzan, David

Subjects
Article Subject
Abstract

The thyroid gland is a known site for metastatic tumors from various primary sites. Thyroid metastases are not an exceptional finding at autopsy, and they are encountered in 2% to 9% of the patients with malignant neoplasm. The most frequent tumors to metastasize are breast, lung, melanoma, and kidney carcinomas. Despite the fact that it is one of the largest vascular organs in the body, clinical and surgical cases have given an incidence of 3% of secondary malignances of this organ. Metastatic colon carcinoma to the thyroid gland has been reported, and it is not as rare as one might think. We present a very unique case of colon carcinoma metastasis to the right thyroid lobe and lung five years after colon resection, with reoccurrence two years later in the contralateral thyroid lobe. The literature regarding colon cancer metastasizing to the thyroid gland was reviewed with an attempt to disclose features of this presentation regarding patient’s prognosis.

Published
2013
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42. Adjuvant Autologous Melanoma Vaccine for Macroscopic Stage III Disease: Survival, Biomarkers, and Improved Response to CTLA-4 Blockade

Author

Lotem, Michal, primary, Merims, Sharon, additional, Frank, Stephen, additional, Hamburger, Tamar, additional, Nissan, Aviram, additional, Kadouri, Luna, additional, Cohen, Jonathan, additional, Straussman, Ravid, additional, Eisenberg, Galit, additional, Frankenburg, Shoshana, additional, Carmon, Einat, additional, Alaiyan, Bilal, additional, Shneibaum, Shlomo, additional, Ozge Ayyildiz, Zeynep, additional, Isbilen, Murat, additional, Mert Senses, Kerem, additional, Ron, Ilan, additional, Steinberg, Hanna, additional, Smith, Yoav, additional, Shiloni, Eitan, additional, Gure, Ali Osmay, additional, and Peretz, Tamar, additional

Published
2016
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47. Parenteral arginine impairs intestinal adaptation following massive small bowel resection in a rat model

Author

Section of Pediatric Surgery, Mott Children???s Hospital, University of Michigan, Ann Arbor, MI, USA, Department of Pathology, Rappaport Faculty of Medicine, Bnai Zion Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Department of Pediatrics, Rappaport Faculty of Medicine, Carmel Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Department of Pediatric Surgery, Rappaport Faculty of Medicine, Bnai Zion Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Department of Surgery B, Rappaport Faculty of Medicine, Carmel Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Ann Arbor, Sukhotnik, Igor, Lerner, Aaron, Mogilner, Jorge G., Coran, Arnold G., Miselevich, Iness, Shiloni, Eitan, Lurie, Michael, Section of Pediatric Surgery, Mott Children???s Hospital, University of Michigan, Ann Arbor, MI, USA, Department of Pathology, Rappaport Faculty of Medicine, Bnai Zion Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Department of Pediatrics, Rappaport Faculty of Medicine, Carmel Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Department of Pediatric Surgery, Rappaport Faculty of Medicine, Bnai Zion Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Department of Surgery B, Rappaport Faculty of Medicine, Carmel Medical Center, Technion-Israel Institute of Technology, 47 Golomb St, P.O. Box 4940, Haifa, 31048, Israel, Ann Arbor, Sukhotnik, Igor, Lerner, Aaron, Mogilner, Jorge G., Coran, Arnold G., Miselevich, Iness, Shiloni, Eitan, and Lurie, Michael

Abstract

The nitric oxide precursor L-arginine (ARG) has been shown to influence intestinal structure and absorptive function. It is also well known that the route of administration modulates the effects of ARG. The present study evaluated the effects of parenteral ARG on structural intestinal adaptation, cell proliferation, and apoptosis in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats were divided into three experimental groups: Sham rats underwent bowel transection and reanastomosis, SBS rats underwent a 75% small bowel resection, and SBS-ARG rats underwent a 75% small bowel resection and were treated with ARG given subcutaneously at a dose of 300????g/kg, once daily, from days 3 to 14. Parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined on day 15 following operation. The SBS rats demonstrated a significant increase in jejunal and ileal bowel and mucosal weight, villus height and crypt depth, and cell proliferation index compared with the sham group. The SBS-ARG animals demonstrated lower ileal bowel and mucosal weights, jejunal mucosal DNA and ileal mucosal protein, and jejunal and ileal villus height and crypt depth compared with SBS animals. The SBS-ARG rats also had a lower cell proliferation index in both jejunum and ileum and a greater enterocyte apoptotic index in ileum compared with the SBS-untreated group. In conclusion, in a rat model of SBS, parenteral arginine inhibits structural intestinal adaptation. Decreased cell proliferation and increased apoptosis are the main mechanisms responsible for decreased cell mass.

Published
2006

48. Effect of leptin on intestinal re-growth following massive small bowel resection in rat

Author

Section of Pediatric Surgery, Mott Children???s Hospital, University of Michigan, Ann Arbor, MI, USA, Department of Pediatric Surgery B, Rappaport Faculty of Medicine, Technion, Bnai Zion Medical Center, 47 Golomb Street, P.O.B. 4940, 31048, Haifa, Israel, Carmel Medical Center, Haifa, Israel, Ann Arbor, Shiloni, Eitan, Lurie, Michael, Vadasz, Zahava, Coran, Arnold G., Hatoum, Ossama A., Mogilner, Jorge G., Sukhotnik, Igor, Section of Pediatric Surgery, Mott Children???s Hospital, University of Michigan, Ann Arbor, MI, USA, Department of Pediatric Surgery B, Rappaport Faculty of Medicine, Technion, Bnai Zion Medical Center, 47 Golomb Street, P.O.B. 4940, 31048, Haifa, Israel, Carmel Medical Center, Haifa, Israel, Ann Arbor, Shiloni, Eitan, Lurie, Michael, Vadasz, Zahava, Coran, Arnold G., Hatoum, Ossama A., Mogilner, Jorge G., and Sukhotnik, Igor

Abstract

Recent evidence suggests that the adipose tissue-derived cytokine leptin (LEP) is involved in modulation of growth and differentiation of normal small intestine. The purpose of the present study was to evaluate the effects of parenteral LEP on structural intestinal adaptation, cell proliferation and apoptosis in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats were divided into three experimental groups: Sham rats underwent bowel transection and re-anastomosis, SBS-rats underwent a 75% small bowel resection, and SBS-LEP-rats underwent bowel resection and were treated with LEP given subcutaneously at a dose of 20????g/kg, once daily, from day 3 through 14. Parameters of intestinal adaptation (bowel and mucosal weights, mucosal DNA and protein, villus height and crypt depth in jejunum and ileum), enterocyte proliferation and enterocyte apoptosis were determined on day 15 following operation. Ileal tissue samples were taken for detection of bax and bcl-2 gene expression using RT-PCR technique. Statistical analysis was performed using the non-parametric Kruskal???Wallis ANOVA test, with P<0.05 considered statistically significant. Treatment with subcutaneous LEP resulted in a significant increase in jejunal (17%, P<0.05) and ileal (13%, P<0.05) bowel weight, jejunal (10%, P<0.05) and ileal (25%, P<0.05) mucosal weight, jejunal (26%, P<0.05) and ileal (38%, P<0.05) mucosal DNA, ileal (25%, P<0.05) mucosal protein, jejunal (41%, P<0.05) and ileal (21%, P<0.05) villus height, jejunal (37%, P<0.05) crypt depth, and jejunal (24%, P<0.05) and ileal (21%, P<0.05) enterocyte proliferation compared to SBS-animals. Enterocyte apoptosis increased significantly after bowel resection in jejunum and ileum compared to sham animals and was accompanied by an increased bax gene expression and a decreased bcl-2 gene expression in ileal samples. SBS-LEP rats showed a trend toward a decrease in enterocyte apoptosis in ileum and a mild decrease in bax gene exp

Published
2006

49. Oral arginine improves intestinal recovery following ischemia-reperfusion injury in rat

Author

Section of Pediatric Surgery, C.S. Mott Children???s Hospital and University of Michigan Medical School, Ann Arbor, MI, USA, Department of Pediatric Surgery B, Rappaport Faculty of Medicine, Technion, Bnai Zion Medical Center, 47 Golomb St., 4940, Haifa, 31048, Israel, Carmel Medical Center, Haifa, Israel, Ann Arbor, Sukhotnik, Igor, Shiloni, Eitan, Mogilner, Jorge G., Helou, Habib, Coran, Arnold G., Lurie, Michael, Bernsteyn, Aleksander, Section of Pediatric Surgery, C.S. Mott Children???s Hospital and University of Michigan Medical School, Ann Arbor, MI, USA, Department of Pediatric Surgery B, Rappaport Faculty of Medicine, Technion, Bnai Zion Medical Center, 47 Golomb St., 4940, Haifa, 31048, Israel, Carmel Medical Center, Haifa, Israel, Ann Arbor, Sukhotnik, Igor, Shiloni, Eitan, Mogilner, Jorge G., Helou, Habib, Coran, Arnold G., Lurie, Michael, and Bernsteyn, Aleksander

Abstract

Arginine and nitric oxide are critical to the normal physiology of the gastrointestinal tract and maintain the mucosal integrity of the intestine in various intestinal disorders. In the present study, we evaluate the effects of oral arginine (ARG) supplementation on intestinal structural changes, enterocyte proliferation, and apoptosis following intestinal ischemia???reperfusion (IR) in the rat. Male Sprague???Dawley rats were divided into three experimental groups: sham rats underwent laparotomy and superior mesenteric artery mobilization, IR rats underwent superior mesenteric artery occlusion for 30??min following by 24??h of reperfusion, and IR-ARG rats were treated with enteral arginine given in drinking water (2%) 48??h before and following IR. Intestinal structural changes, enterocyte proliferation, and enterocyte apoptosis were determined 24??h following IR. A nonparametric Kruskal???Wallis ANOVA test was used for statistical analysis with p <0.05 considered statistically significant. IR rats demonstrated a significant decrease in bowel weight in duodenum and jejunum, mucosal weight in jejunum and ileum, and villus height in jejunum and ileum compared with control animals. IR rats also had a significantly lower cell proliferation index in jejunum and ileum and a higher apoptotic index in ileum compared with control rats. IR-ARG animals demonstrated greater duodenal and jejunal bowel weight; duodenal, jejunal, and ileal mucosal weight; and jejunal and ileal cell proliferation index compared with IR animals. In conclusion, oral ARG administration improves mucosal recovery following IR injury in the rat.

Published
2006

50. Lipopolysaccharide endotoxemia reduces cell proliferation and decreases enterocyte apopotosis during intestinal adaptation in a rat model of short-bowel syndrome

Author

Section of Pediatric Surgery, C.S. Mott Children's Hospital and University of Michigan Medical School, Ann Arbor, MI, USA, Rappaport Faculty of Medicine, Technion, Carmel Medical Center, HaEmek, Medical Center and Rambam Medical Center, Haifa, Israel, Ann Arbor, Sukhotnik, Igor, Yakirevich, E., Shiloni, Eitan, Krausz, M., Kramer, Alexander, Siplovich, L., Coran, Arnold G., Sabo, E., Section of Pediatric Surgery, C.S. Mott Children's Hospital and University of Michigan Medical School, Ann Arbor, MI, USA, Rappaport Faculty of Medicine, Technion, Carmel Medical Center, HaEmek, Medical Center and Rambam Medical Center, Haifa, Israel, Ann Arbor, Sukhotnik, Igor, Yakirevich, E., Shiloni, Eitan, Krausz, M., Kramer, Alexander, Siplovich, L., Coran, Arnold G., and Sabo, E.

Abstract

Sepsis is frequently associated with or complicates short-bowel syndrome (SBS). To investigate the effects of lipopolysaccharide (LPS) endotoxemia on enterocyte proliferation and death via apoptosis in a rat model of SBS, adult male Sprague-Dawley rats were divided into three experimental groups: sham rats underwent bowel transection and reanastomosis; SBS rats underwent 75% small-bowel resection; and SBS-LPS rats underwent 75% bowel resection and were given intraperitoneal injections of LPS 10??mg/kg. Parameters of intestinal adaptation (bowel and mucosal weights, mucosal DNA and protein, villus height, and crypt depth), enterocyte proliferation, and death via apoptosis were determined on day 15 after the operation. Statistical analysis was determined by Student's and ANOVA tests with a P less than 0.05 considered significant. SBS-LPS animals demonstrated a significant decrease (vs SBS rats) in duodenal (20%), jejunal (30%), and ileal (15%) overall weight, duodenal (20%), jejunal (27%), and ileal (18%) mucosal weight, jejunal (20%) and ileal (30%) mucosal DNA, jejunal (29%) and ileal (31%) villus height, and jejunal (14%) and ileal (29%) crypt depth. LPS endotoxemia led to reduced cell proliferation and enterocyte apoptosis compared to untreated SBS animals. Thus, in a rat model of SBS, LPS endotoxemia inhibits intestinal adaptation. A possible mechanism may be decreased cell proliferation. Decreased enterocyte loss via apoptosis may reflect a reduced number of enterocytes. Other mechanisms (necrosis) may be mainly responsible for cell death following LPS injection.

Published
2006

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