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RNA viruses have been the most destructive due to their transmissibility and lack of control measures. Developments of vaccines for RNA viruses are very tough or almost impossible as viruses are highly mutable. For the last few decades, most of the epidemic and pandemic viral diseases have wreaked huge devastation with innumerable fatalities. To combat this threat to mankind, plant-derived novel antiviral products may contribute as reliable alternatives. They are assumed to be nontoxic, less hazardous, and safe compounds that have been in uses in the beginning of human civilization. In this growing COVID-19 pandemic, the present review amalgamates and depicts the role of various plant products in curing viral diseases in humans.

Titanium(IV) complexes of type[(η5-C5H5)2TiCl(L)] have been synthesized by the reactions of bis(cyclopentadienyl)titanium(IV)dichloride with Schiff bases (LH) derived by the condensation of 5-(substituted aryl)-2-hydrazino-1,3,4-oxadiazole and indoline-2,3-dione in tetrahydrofuranin the presence oftriethylamine. All these complexes are soluble in PhNO2, DMF and DMSO.The complexes were characterized by elemental analyses, electrical conductance, magnetic susceptibility, UV-Vis, IR, 1H NMR, 13C NMR, XRD and SEM spectral techniques. Low molar conductance values indicate that they are non-electrolytes. The spectral data indicate5-coordinate geometry for the complexes.XRD pattern indicate that the complexes have monoclinic crystal system and particle sizes were found 49.36 nm (nano-size). In vitro antifungal activity of synthesized compounds was evaluated against fungi Aspergillusniger, Aspergillusflavus,ColletotrichumfalcatumandIn vitro antibacterial activity was determined by screening the compounds against gram negative (P. aeruginosa, S.typhi) and gram positive (S. aureus and B.subtilis) bacterial strains using minimum inhibition concentration method (MIC) by serial dilution technique. The titanocene(IV) complexes have higher antimicrobial effect than the parent Schiff bases.

Nature‐based solutions (NbS)—solutions to societal challenges that involve working with nature—have recently gained popularity as an integrated approach that can address climate change and biodiversity loss, while supporting sustainable development. Although well‐designed NbS can deliver multiple benefits for people and nature, much of the recent limelight has been on tree planting for carbon sequestration. There are serious concerns that this is distracting from the need to rapidly phase out use of fossil fuels and protect existing intact ecosystems. There are also concerns that the expansion of forestry framed as a climate change mitigation solution is coming at the cost of carbon rich and biodiverse native ecosystems and local resource rights. Here, we discuss the promise and pitfalls of the NbS framing and its current political traction, and we present recommendations on how to get the message right. We urge policymakers, practitioners and researchers to consider the synergies and trade‐offs associated with NbS and to follow four guiding principles to enable NbS to provide sustainable benefits to society: (1) NbS are not a substitute for the rapid phase out of fossil fuels; (2) NbS involve a wide range of ecosystems on land and in the sea, not just forests; (3) NbS are implemented with the full engagement and consent of Indigenous Peoples and local communities in a way that respects their cultural and ecological rights; and (4) NbS should be explicitly designed to provide measurable benefits for biodiversity. Only by following these guidelines will we design robust and resilient NbS that address the urgent challenges of climate change and biodiversity loss, sustaining nature and people together, now and into the future.

Background: In the current scenario, the urbanization is happening at a faster pace and the economy is not able to match at par with urbanization, this In turn is causing problems of unavailability of safe and potable water along with proper sewage system, despecially in urban slums. As a result of the scenario many urban slums are still using poor quality pit latrines and even drawing water from nearby wells, water taps which are not complying with government norms. Space is also a big problem which is being faced by these slums, due to which there is a lack of enough spaces in the houses and nearby areas, therby causing overcrowding. Due to overcrowding, the space between houses, pit latrines, wells, taps and water bodies has decreased and thus the potential of contamination of water bodies by fecal microorganisms has increased drastically. Given the above knowledge, a study is much needed to estimate the presumptive and differential coliform count of water samples from the urban slum area in Lucknow. Subjects & Methods: A cross-sectional survey was conducted during the period of January to June 2019. Samples were collected from all the taps, hand pumps that were currently in use, along with potable water stored in households of Sarvodaya Nagar (an urban slum area), Lucknow and were processed in the Microbiology department of IIMS&R, Lucknow. Results: A total of 53(63%) samples were taken from Public supply, out of which 36(67. 9%) were found unsatisfactory and 17(32.0%) were intermediate, were found to be contaminated with Pseudomonas sp., E.coli, Thermotolerent E.coli, Klebsiella Spp. Citrobacter, Enterococcus and Pseudornonas Spp., E.coli, Thermotolerent E.coli, Klebsiella respectively. In the study more than half of the samples were taken from the Public water supply. Out of these about seventy percent were unsatisfactory and were found to be contaminated with Pseudomonas. Conclusion: The proper sanitation, regular treatment, supervision of water sources, arid regular bacteriological assessment of all water sources for drinking should be planned and conducted.

Aim: The present study aimed to evaluate the prognostic scoring system for fetal echogenic kidneys to diagnose the fetal kidney diseases in mid trimester by determining the kidney volume, laterality, bladder size, amniotic fluid and other associated anomalies. Methodology: 27 fetuses diagnosed with the “Echogenic kidneys” were enrolled in the study based on subjective assessment in sonography. The scoring was performed based on the certain parameters including renal size, laterality, bladder size, amniotic fluid and associated anomalies. The prognosis of the live births was assessed during the post natal life. Results: Out of 27 fetuses enrolled in the present study, 11 were live births, 9 were NND, IUD and miscarriage and 7 were terminations. 10 live births had the scoring of 4, 5 and 6 and 1 live birth had a scoring of 8. Conclusion: Within the limitations of the study we can conclude that the scoring system could be a good tool to identify the anomalies in the fetal kidneys during the mid-trimester. With the identification of the fetal echogenic kidneys the prognosis can be ruled out which helps in easing out the parental anxiety and renders them correct advice to opt for termination or not.

There has been a spurt in the spread of microbial resistance to antibiotics due to indiscriminate use of antimicrobial agents in human medicine, agriculture, and animal husbandry. It has been realized that conventional antibiotic therapy would be less effective in the coming decades and more emphasis should be given for the development of novel antiinfective therapies. Cysteine rich peptides (CRPs) are broad-spectrum antimicrobial agents that modulate the innate immune system of different life forms such as bacteria, protozoans, fungi, plants, insects, and animals. These are also expressed in several plant tissues in response to invasion by pathogens, and play a crucial role in the regulation of plant growth and development. The present work explores the importance of CRPs as potent antimicrobial agents, which can supplement and/or replace the conventional antibiotics. Different plant parts of diverse plant species showed the presence of antimicrobial peptides (AMPs), which had significant structural and functional diversity. The plant-derived AMPs exhibited potent activity toward a range of plant and animal pathogens, protozoans, insects, and even against cancer cells. The cysteine-rich AMPs have opened new avenues for the use of plants as biofactories for the production of antimicrobials and can be considered as promising antimicrobial drugs in biotherapeutics.

This paper examines the water–energy–food (WEF) nexus in a humanitarian context, with a specific focus on water, sanitation and hygiene (WASH). It highlights the complex and non-linear interactions that WASH has with other areas of the WEF nexus. In doing so, it blends the social dimensions (access, safety, consumption, and use) with the WEF resource dimensions (availability and resource sustainability), including a further emphasis on sanitation as a key, but often ignored, element of the WEF nexus. Drawing on the case of the Rohingya refugee camps in Bangladesh, we examine how household-level access to WASH shapes and is shaped by use, access, and availability of energy and food, and finally their effects on host–refugee interactions. We find that there are implicit and explicit links between WASH and WEF. Moreover, any small intervention in any of the WEF areas has positive knock-on effects on the other resources, especially in enhancing resource access and use. We conclude that bottom-up perspectives on these interlinkages with active participation from both host and refugee households are required to understand the implicit and explicit connections across WASH and the WEF nexus in humanitarian contexts. We also argue that sanitation is a key element of the WEF nexus and should not be ignored within the predominant resource-centric framing of the WEF.

Lipases are important biocatalysts having the third largest global demand after amylases and proteases. In the present study, we have screened 56 potential lipolytic Pseudomonas strains for their lipolytic activity. Pseudomonas plecoglossicida S7 showed highest lipase production with specific activity of 70 U/mg. Statistical optimizations using Plackett Burman design and response surface methodology evaluated fourteen different media supplements including various oilcakes, carbon sources, nitrogen sources, and metal ions which led to a 2.23-fold (156.23 U/mg) increase in lipase activity. Further, inoculum size optimization increased the overall lipase activity by 2.81-folds. The lipase was active over a range of 30-50° C with a pH range (7-10). The enzyme was tolerant to various solvents like chloroform, methanol, 1-butanol, acetonitrile, and dichloromethane and retained 60% of its activity in the presence of sodium dodecyl sulfate (0.5% w/v). The enzyme was immobilized onto Ca-alginate beads which increased thermal (20-60 °C) and pH stability (5-10). The purified enzyme could successfully remove sesame oil stains and degraded upto 25.2% of diesel contaminated soil. These properties of the lipase will help in its applicability in detergent formulations, wastewater treatments, and biodegradation of oil in the environment.

Lignocellulosic materials form the building block of 50% of plant biomass comprising non-chewable agri-components like wheat straw, rice stubbles, wood shavings and other crop residues. The degradation of lignin, cellulose and hemicellulose is complicated and presently being done by chemical process for industrial application through a very energy intensive process. Lignin degradation is primarily an oxidative process where the enzyme lignin peroxidase digests the polymer into smaller fragments. Being a recalcitrant component, higher lignin content poses a challenge of lower recovery of product for industrial use. Globally, the scientists are working on leveraging fungal biotechnology for using the lignocellulose degrading enzymes secreted by actinomycetes and basidiomycetes fungal groups. Enzymes contributing to degradation of lignin are mainly performing the function of modifying the lignin and degrading the lignin. Ligninolytic enzymes do not act as an independent reaction but are vital to complete the degradation process. Microbial enzyme technology is an emerging green tool in industrial biotechnology for commercial application. Bioprocessing of lignocellulosic biomass is challenged by limitations in enzymatic and conversion process where pretreatment and separation steps are done to remove lignin and hydrolyze carbohydrate into fermentable sugars. This review highlights recent advances in molecular biotechnology, lignin valorization, sequencing, decipher microbial membership, and characterize enzyme diversity through 'omics' techniques. Emerging techniques to characterize the interwoven metabolism and spatial interactions between anaerobes are also reviewed, which will prove critical to developing a predictive understanding of anaerobic communities to guide in microbiome engineering This requires more synergistic collaborations from microbial biotechnologists, bioprocess engineers, enzymologists, and other biotechnological fields.

Introduction Control of infections in the operation theater (OT) is of utmost importance. Microbiological surveillance is an effective tool for identifying and controlling infections. The purpose of this study was to investigate the prevalence rate of microorganisms in OTs, to identify the type of microorganisms, and to detect contamination of various surfaces and air of OT. Methods OTs were properly cleaned with soap and water. All surfaces were disinfected, followed by fumigation with quaternary ammonium compounds. OTs were kept closed overnight. In the morning, they were opened, and samples were collected, taking all aseptic precautions. The settle plate method was used for air sampling, and the swab method was used for surface sampling. Samples were collected from four surfaces of OTs, i.e., floor, wall, table, and light, and samples of the OT air were also collected and immediately transported to the microbiology laboratory of the institution in sterile conditions. Result A total of 1640 swab samples were taken from eight OTs, out of which 487 (29.7%) were found positive for bacterial growth. Most of them were non-pathological microorganisms such as aerobic spore-forming Bacilli and Micrococcus. Among various OTs, septic OT showed the highest bacterial growth (82 positive cultures out of 200). In the surface sampling of various OTs, aerobic spore-forming Bacilli (221/487) was the most common isolate, followed by coagulase-negative Staphylococci (74/487), and Micrococcus (67/487). General surgery, septic, and emergency OTs had maximum air bioload (97, 93, and 91 colony-forming unit (CFU)/M3, respectively). Conclusion In surface sampling of OTs, it was found that septic OT and general surgery OT were most contaminated where the patient load was high. Among all the surfaces, OT walls and tables were most contaminated with pathogenic microorganisms. The average air bioload of all OTs was ranged between 79 and 97 CFU/M3.