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124 results on '"Shin, Yeun-Kyung"'

1. Neurologic Effects of SARS-CoV-2 Transmitted among Dogs

Author

Kim, Dong-Hwi, Kim, Da-Yoon, Kim, Kyu-Sung, Han, Sang-Hoon, Go, Hyeon-Jeong, Kim, Jae-Hyeong, Lim, Kyu-Beom, Lee, Dong- Hun, Lee, Joong-Bok, Park, Seung-Yong, Song, Chang-Seon, Lee, Sang-Won, Choi, Yang-Kyu, Shin, Yeun-Kyung, Kwon, Oh-Kyu, Kim, Do-Geun, and Choi, In-Soo

Subjects
Brain research -- Research -- Health aspects, Medical research -- Health aspects, Medicine, Experimental -- Health aspects, Nervous system diseases -- Development and progression -- Research, Dogs -- Health aspects -- Research, Infection -- Development and progression -- Research, Proteins -- Health aspects -- Research, Health
Abstract

Since SARS-CoV-2 was first reported in late 2019, infection has been observed primarily in humans; however, animals of various species have also been infected, partially because their angiotensin-converting enzyme 2 [...]

Published
2023
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3. Genomic Epidemiology of African Swine Fever Virus Identified in Domestic Pig Farms in South Korea during 2019–2021

Author

Kwon, Oh-Kyu, primary, Kim, Da-Won, additional, Heo, Jin-Hwa, additional, Kim, Ji-Yun, additional, Nah, Jin-Ju, additional, Choi, Ji-Da, additional, Lee, Dong-Wook, additional, Cho, Ki-Hyun, additional, Hong, Seong-Keun, additional, Kim, Yeon-Hee, additional, Kang, Hae-Eun, additional, Kwon, Jung-Hoon, additional, and Shin, Yeun-Kyung, additional

Published
2024
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4. Development and evaluation of a triplex real-time quantitative reverse transcription-polymerase chain reaction for rapid and differential detection of three feline respiratory viral pathogens

Author

Baek, Ji-Su, primary, Kim, Jong-Min, additional, Kim, Hye-Ryung, additional, Park, Ji-Hoon, additional, Shin, Yeun-Kyung, additional, Kang, Hae-Eun, additional, Kwon, Jung-Hoon, additional, Lee, Won-Jae, additional, Jang, Min, additional, Lee, Sang-Kwon, additional, Cho, Ho-Seong, additional, Oh, Yeonsu, additional, Kwon, Oh-Deog, additional, and Park, Choi-Kyu, additional

Published
2023
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5. Complete Genomic Characterization of Lumpy Skin Disease Virus Isolates from Beef Cattle in Lopburi Province, Central Thailand, during 2021–2022

Author

Suwankitwat, Nutthakarn, primary, Deemagarn, Taweewat, additional, Bhakha, Kultyarat, additional, Songkasupa, Tapanut, additional, Vitoonpong, Ratchaneekorn, additional, Trakunjaroonkit, Pannaporn, additional, Rodphol, Sureenipa, additional, Nuansrichay, Bandit, additional, Chintapitaksakul, Lerdchai, additional, Wongsarattanasin, Khanin, additional, Kwon, Oh-Kyu, additional, Kang, Hae-Eun, additional, and Shin, Yeun-Kyung, additional

Published
2023
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7. Epidemiological and Molecular Approaches for a Fatal Feline Panleukopenia Virus Infection of Captive Siberian Tigers (Panthera tigris altaica) in the Republic of Korea

Author

Yeo, Yong-Gu, primary, Kim, Hye-Ryung, additional, Park, Jonghyun, additional, Kim, Jong-Min, additional, Shin, Yeun-Kyung, additional, Lee, Kyoung-Ki, additional, Kwon, Oh-Kyu, additional, Jeoung, Hye-Young, additional, Kang, Hae-Eun, additional, Ku, Bok-Kyung, additional, Park, Seung-Chun, additional, Kwon, Oh-Deog, additional, and Park, Choi-Kyu, additional

Published
2023
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8. Complete Genomic Characterization of Lumpy Skin Disease Virus Isolates from Beef Cattle in Lopburi Province, Central Thailand, during 2021–2022.

Author

Suwankitwat, Nutthakarn, Deemagarn, Taweewat, Bhakha, Kultyarat, Songkasupa, Tapanut, Vitoonpong, Ratchaneekorn, Trakunjaroonkit, Pannaporn, Rodphol, Sureenipa, Nuansrichay, Bandit, Chintapitaksakul, Lerdchai, Wongsarattanasin, Khanin, Kwon, Oh-Kyu, Kang, Hae-Eun, and Shin, Yeun-Kyung

Subjects
LUMPY skin disease, VIRUS diseases, BEEF cattle, WHOLE genome sequencing, SINGLE nucleotide polymorphisms
Abstract

Simple Summary: The study focuses on the genetic characteristics of the lumpy skin disease virus (LSDV) from non-vaccinated beef cattle with LSD symptoms that lived in areas that had received live-attenuated vaccines in Lopburi, Thailand. Four skin positive samples through real-time PCR underwent virus isolation on cell cultures, followed by whole genome sequencing. The phylogenetic and mutation analyses were used to compare sequences between four LSDV samples from Lopburi with the LSDV/Thailand/YST/2021 strain (original strain in Thailand). In conclusion, four LSDVs in Lopburi during 2021–2022 were recombinant strains, clade 2.5, same as the LSDV/Thailand/YST/2021 strain. However, the viruses had mutations of open reading frame (ORF) 023 with significant amino acid deletion in two samples from Chai Badan district and of the untranslated region between ORF022 and ORF023 in two samples from Lam Sothi district. Lumpy skin disease (LSD) is a viral infection that impacts the cattle industry. The most efficient approach to prevent disease involves the utilization of live-attenuated LSD vaccines (LAVs), which stands out as the most successful method. However, LAVs might be subjected to changes to their genomes during replication that increase viral infectivity or virulence. The objective of this study was to monitor alterations in the genetic characteristics of the lumpy skin disease virus (LSDV) in beef cattle following the administration of LAVs in Lopburi Province of Central Thailand. A total of four skin samples from LSD cases were collected from non-vaccinated animals that exhibited LSD clinical symptoms from two distinct districts, spanning three subdistricts within the region. The samples of cattle were analyzed using real-time PCR targeting the LSDV074 p32 gene, the virus was isolated, and the entire genome sequences were evaluated through a single nucleotide polymorphisms (SNPs) analysis, and phylogenetic trees were assembled. The investigations revealed that LSDVs from two isolates from Chai Badan district exhibited significant mutations in the open reading frame (ORF) 023 putative protein, while another two isolates from Lam Sonthi district had a change in the untranslated region (UTR). For a result, the most proficient disease diagnosis and control should be evaluated on viral genetics on a regular basis. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Prevalence of Bordetella bronchiseptica, Mycoplasma felis, and Chlamydia felis using a newly developed triplex real-time polymerase chain reaction assay in Korean cat population

Author

Kim, Hye-Ryung, primary, Jeon, Gyu-Tae, additional, Kim, Jong-Min, additional, Baek, Ji-Su, additional, Shin, Yeun-Kyung, additional, Kwon, Oh-Kyu, additional, Kang, Hae-Eun, additional, Cho, Ho-Seong, additional, Cheon, Doo-Sung, additional, and Park, Choi-Kyu, additional

Published
2022
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15. Simple and Rapid Colorimetric Detection of Canine Parainfluenza Virus 5 (Orthorubulavirus mammalis) Using a Reverse-Transcription Loop-Mediated Isothermal Amplification Assay.

Author

Kim, Jong-Min, Kim, Hye-Ryung, Baek, Ji-Su, Kwon, Oh-Kyu, Kang, Hae-Eun, Shin, Yeun-Kyung, and Park, Choi-Kyu

Subjects
PARAINFLUENZA viruses, AMPLIFICATION reactions, GENE amplification, POLYMERASE chain reaction, DETECTION limit
Abstract

Despite its many advantages, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay has yet to be developed for canine parainfluenza virus 5 (CPIV5). In this study, a visual RT-LAMP (vRT-LAMP) assay was developed for the rapid detection of CPIV5 in clinical samples. At a constant reaction temperature of 62 °C, the assay was completed within 40 min, and the results could be directly detected with the naked eye using a hydroxynaphthol blue (HNB) metal indicator without any additional detection apparatuses. The assay specifically amplified CPIV5 RNA with a limit of detection of 10 RNA copies/reaction, which was 10-fold more sensitive than the previously reported conventional reverse-transcription polymerase chain reaction (cRT-PCR) assay and was comparable to the previously reported real-time RT-PCR (qRT-PCR) assay. In a clinical evaluation using 267 nasopharyngeal swab samples collected from hospitalized dogs with respiratory symptoms, the CPIV5 detection rate using the vRT-LAMP assay was 5.24% (14/267), which was higher than that of the cRT-PCR assay (4.49%, 12/267) and consistent with that of the qRT-PCR assay, demonstrating 100% concordance with a kappa coefficient value (95% confidence interval) of 1 (1.00–1.00). The discrepancies in the results of the assays were confirmed to be attributed to the low sensitivity of the cRT-PCR assay. Owing to the advantages of a high specificity, rapidity, and simplicity, the developed vRT-LAMP assay using an HNB metal indicator will be a valuable diagnostic tool for the detection of CPIV5 in canine clinical samples, even in resource-limited laboratories. [ABSTRACT FROM AUTHOR]

Published
2023
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19. Whole Genome Sequencing of SARS-CoV-2 in Cats and Dogs in South Korea in 2021.

Author

Shin, Yeun-Kyung, Kwon, Oh-Kyu, Heo, Jinhwa, Nah, Jinju, Kang, Hae-Eun, Kang, Yunhee, Song, In Jun, and Sung, Ho-Kyung

Subjects
WHOLE genome sequencing, SARS-CoV-2 Delta variant, SARS-CoV-2, PETS, DOGS, CATS
Abstract

Simple Summary: Forty SARS-CoV-2-positive swab samples from 40 animals owned by confirmed SARS-CoV-2-infected owners in Korea in 2021 were analyzed using next-generation sequencing (NGS) to identify the genomic lineage of the viruses. Among the 40 animal samples, eight Pango lineages (B.1.1.7) (Alpha variant), B.1.429 (Epsilon variant), B.1.470, B.1.497, B.1.619.1, B.1.620, AY.69 (Delta variant), and AY.122.5 (Delta variant) were identified. This study provides the first reported cases of six lineages (B.1.470, B.1.497, B.1.620, B.1.619.1, AY.69 (Delta variant), and AY.122.5 (Delta variant)) in cats and dogs. Our results emphasize the importance of monitoring SARS-CoV-2 in pets because they are dynamic hosts of variant Pango lineages of SARS-CoV-2. SARS-CoV-2 infections have caused unprecedented damage worldwide by affecting humans and various animals. The first reported animal infection was observed in a pet dog in Hong Kong in March 2020. 36 countries reported 692 SARS-CoV-2 infections in 25 different animal species by 31 August 2022. Most outbreaks were caused by contact with SARS-CoV-2 infected humans. In South Korea, the first SARS-CoV-2 infection in an animal was reported in a cat in February 2021. As of 31 December 2021, 74 dogs and 42 cats have been confirmed to have SARS-CoV-2 in South Korea. Here, we identified various SARS-CoV-2 genomic lineages in SARS-CoV-2 confirmed cats and dogs. Among the 40 animal samples sequenced for lineage identification, a total of eight Pango lineages (B.1.1.7 (Alpha variant), B.1.429 (Epsilon variant), B.1.470, B.1.497, B.1.619.1, B.1.620, AY.69 (Delta variant), and AY.122.5 (Delta variant)) were identified. The dominant lineages were AY.69 (Delta variant; 37.5%), B.1.497 (35.0%), and B.1.619.1 (12.5%). This study provides the first reported cases of six lineages (B.1.470, B.1.497, B.1.620, B.1.619.1, AY.69 (Delta variant)), and AY.122.5 (Delta variant) in cats and dogs. Our results emphasize the importance of monitoring SARS-CoV-2 in pets because they are dynamic hosts of variant Pango lineages of SARS-CoV-2. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Reemergence of foot-and-mouth disease, South Korea, 2000-2011

Author

Park, Jong-Hyeon, Lee, Kwang-Nyeong, Kim, Su-Mi, Lee, Hyang-Sim, Ko, Young- Joon, Tark, Dong-Seob, Shin, Yeun-Kyung, Seo, Min-Goo, and Kim, Byounghan

Subjects
Foot-and-mouth disease -- Analysis, International trade -- Analysis, International trade, Health
Abstract

Foot-and-mouth disease is an infectious viral disease that occurs in animals and is easily transmissible. Outbreaks of this disease affect international trade (1). Since 2000, five outbreaks (in March 2000, [...]

Published
2014
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22. Control of foot-and-mouth disease during 2010-2011 epidemic, South Korea

Author

Park, Jong-Hyeon, Lee, Kwang-Nyeong, Ko, Young-Joon, Kim, Su-Mi, Lee, Hyang-Sim, Shin, Yeun-Kyung, Sohn, Hyun-Joo, Park, Jee-Yong, Yeh, Jung-Yong, Lee, Yoon-Hee, Kim, Min-Jeong, Joo, Yi-Seok, Yoon, Hachung, Yoon, Soon-Seek, Cho, In-Soo, and Kim, Byounghan

Subjects
Vaccination -- Health aspects, Foot-and-mouth disease -- Development and progression -- Health aspects -- Control, Infection -- Development and progression -- Health aspects, Health
Abstract

Foot-and-mouth disease (FMD) is a highly contagious disease caused by foot-and-mouth disease virus (FMDV; family Picornaviridae, genus Aphthovirus). FMDV serotypes O, A, and Asia 1 are widespread in Southeast Asia [...]

Published
2013
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