1. Enhanced osteogenic differentiation of human mesenchymal stem cells on Ti surfaces with electrochemical nanopattern formation.
- Author
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Shin, Yong Cheol, Pang, Kang-Mi, Han, Dong-Wook, Lee, Kyeong-Hee, Ha, Yoon-Cheol, Park, Jun-Woo, Kim, Bongju, Kim, Doohun, and Lee, Jong-Ho
- Subjects
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DENTAL metallurgy , *NANOTUBES , *MESENCHYMAL stem cell differentiation - Abstract
Abstract Titanium (Ti) and its alloys are mainly used for dental and orthopedic applications due to their excellent biocompatibility and mechanical properties. However, their intrinsic bioinertness often quotes as a common complaint for biomedical applications. Herein, we produced nanopattern Ti surfaces with 10 nm nanopores in 120 nm dimples by electrochemical nanopattern formation (ENF), and evaluated the osteogenic differentiation of human mesenchymal stem cells (hMSCs) on the nanopattern Ti surfaces. The ENF surfaces were obtained by removing the TiO 2 nanotube (NT) layers prepared by an anodization process. To determine the in vitro effects of the ENF surface, cell proliferation assay, alkaline phosphatase activity assay, alizarin red staining, western blotting, and immunocytochemistry were performed. Atomic force microscopy and scanning electron microscopy analysis show that the ENF surface has an ultrafine surface roughness with highly aligned nanoporous morphology. hMSCs on ENF surfaces exhibit increased proliferation and enhanced osteogenic differentiation as compared to the ordered TiO 2 nanotubular and compact TiO 2 surfaces. Surface modification with the ENF process is a promising technique for fabricating osteointegrative implant materials with a highly bioactive, rigid and purified nano surfaces. Highlights • Ultrafine nano-roughened structure is formed by ENF process. • ENF process leads to the formation of multi-nanoscale structures on Ti sheets. • ENF surfaces can simultaneously promote proliferation and osteodifferentiation. • ENF process is a promising technique for fabricating medical implant devices. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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