82 results on '"Sho Nishida"'
Search Results
2. Metabolomic Profiling of Mice with Tacrolimus-Induced Nephrotoxicity: Carnitine Deficiency in Renal Tissue
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Sho Nishida, Tamaki Ishima, Natsuka Kimura, Daiki Iwami, Ryozo Nagai, Yasushi Imai, and Kenichi Aizawa
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metabolome ,tacrolimus ,chronic kidney disease ,metabolite ,histamine ,tacrolimus induced nephrotoxicity ,Biology (General) ,QH301-705.5 - Abstract
Tacrolimus (TAC)-induced chronic nephrotoxicity (TAC nephrotoxicity) has a detrimental effect on long-term kidney graft survival. However, the pathogenesis of TAC nephrotoxicity remains largely unknown. We explored it by focusing on metabolic changes in renal tissues. In this study, mice were separated into TAC and control groups (n = 5/group). TAC was administered to the TAC group (1 mg/kg/d for 28 days) subcutaneously. The control group was similarly treated with normal saline. Renal tissue metabolomes were evaluated. Renal fibrosis was observed only in the TAC group. Metabolomic analysis showed that carnitine and related metabolites were substantially lower in the TAC group than in the control group, presumably due to impaired biosynthesis and reabsorption. Low carnitine levels impair antioxidation in renal tissues and β-oxidation in mitochondria, which may lead to renal tissue damage. This metabolomic analysis revealed that carnitine deficiency in renal tissue appears to explain TAC nephrotoxicity.
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- 2024
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3. Long-term impact of baseline serum uric acid levels on living kidney donors: a retrospective study
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Kosuke Tanaka, Shigeyoshi Yamanaga, Yuji Hidaka, Sho Nishida, Kohei Kinoshita, Akari Kaba, Toshinori Ishizuka, Satoshi Hamanoue, Kenji Okumura, Chiaki Kawabata, Mariko Toyoda, Akira Miyata, Masayuki Kashima, and Hiroshi Yokomizo
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Serum uric acid ,Living kidney donors ,Adverse events ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Background Preoperative characteristics of living kidney donors are commonly considered during donor selection and postoperative follow-up. However, the impact of preoperative uric acid (UA) levels is poorly documented. The aim of this study was to evaluate the association between preoperative serum UA levels and post-donation long-term events and renal function. Methods This was a single-center retrospective analysis of 183 living kidney donors. The donors were divided into high (≥5.5 mg/dl) and low (
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- 2021
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4. Development of strangulation ileus associated with the ureter of the transplanted kidney 18 years after kidney transplantation
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Toshihiro Shimizu, Saki Katano, Sho Nishida, Yoshitaka Kinoshita, Takahiro Shinzato, Yasunaru Sakuma, and Daiki Iwami
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kidney transplantation ,strangulation ileus ,surgical complication ,ureteroneocystostomy ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Introduction After kidney transplantation, patients should be treated with caution and monitored for surgical complications. Among the possible surgical complications, strangulation ileus after kidney transplantation is rare. Case presentation A 59‐year‐old woman who had undergone kidney transplantation at 41 years of age presented to our hospital with lower abdominal pain. She was diagnosed with strangulation ileus and underwent emergency surgery. In the lower right abdomen, the small intestine was compressed by cord‐like tissue running from the intraperitoneal space to the retroperitoneal space. We confirmed that the cord‐like tissue was the ureter of the transplanted kidney. The necrotic small intestine was resected, and ureter‐ureteral anastomosis of the ureter of the transplanted kidney was performed. Conclusion All surgical procedures, including ureteroneocystostomy, require careful attention. The occurrence of some postoperative surgical complications can be prevented by carefully performing the kidney transplantation procedure.
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- 2021
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5. Factors related to suboptimal recovery of renal function after living donor nephrectomy: a retrospective study
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Sho Nishida, Yuji Hidaka, Mariko Toyoda, Kohei Kinoshita, Kosuke Tanaka, Chiaki Kawabata, Satoshi Hamanoue, Akito Inadome, Hiroshi Yokomizo, Asami Takeda, Soichi Uekihara, and Shigeyoshi Yamanaga
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Renal function ,Living donor ,Kidney transplant ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Background The renal function of the remaining kidney in living donors recovers up to 60~70% of pre-donation estimated-glomerular filtration rate (eGFR) by compensatory hypertrophy. However, the degree of this hypertrophy varies from donor to donor and the factors related to it are scarcely known. Methods We analyzed 103 living renal transplantations in our institution and divided them into two groups: compensatory hypertrophy group [optimal group, 1-year eGFR ≥60% of pre-donation, n = 63] and suboptimal compensatory hypertrophy group (suboptimal group, 1-year eGFR
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- 2019
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6. Hypophysitis induced by ipilimumab and nivolumab combination therapy for advanced renal cell carcinoma: A case report
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Aoi Motonaga, Shotaro Nakanishi, Kei Tanaka, Sho Nishida, Keiichiro Izumi, and Seiichi Saito
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Hypophysitis ,Ipilimumab-Nivolumab ,Renal cell carcinoma ,Headache ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Ipilimumab and nivolumab combination therapy is effective against unresectable or metastatic renal cell carcinoma. However, it is associated with many immune-related adverse events, including hypophysitis that is difficult to diagnose early because of non-specific initial symptoms. Herein, we report the case of a 54-year-old man with metastatic renal cell carcinoma who developed hypophysitis after receiving ipilimumab and nivolumab combination therapy. The initial symptom was headache. However, endocrine tests showed decreased levels of cortisol, free thyroxine and thyroid-stimulating hormone. Moreover, magnetic resonance imaging revealed pituitary enlargement. Accordingly, we diagnosed hypophysitis and immediately started hydrocortisone replacement therapy, which improved the symptoms.
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- 2021
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7. Elevated Expression of Vacuolar Nickel Transporter Gene IREG2 Is Associated With Reduced Root-to-Shoot Nickel Translocation in Noccaea japonica
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Sho Nishida, Ryoji Tanikawa, Shota Ishida, Junko Yoshida, Takafumi Mizuno, Hiromi Nakanishi, and Naoki Furuta
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iron regulated 2 ,metal hyperaccumulator ,nickel ,nicotianamine ,Noccaea ,phytoremediation ,Plant culture ,SB1-1110 - Abstract
A number of metal hyperaccumulator plants, including nickel (Ni) hyperaccumulators, have been identified in the genus Noccaea. The ability to accumulate Ni in shoots varies widely among species and ecotypes in this genus; however, little is known about the molecular mechanisms underlying this intra- and inter-specific variation. Here, in hydroponic culture, we compared Ni accumulation patterns between Noccaea japonica, which originated in Ni-enriched serpentine soils in Mt. Yubari (Hokkaido, Japan), and Noccaea caerulescens ecotype Ganges, which originated in zinc/lead-mine soils in Southern France. Both Noccaea species showed extremely high Ni tolerance compared with that of the non-accumulator Arabidopsis thaliana. But, following treatment with 200 μM Ni, N. caerulescens showed leaf chlorosis, whereas N. japonica did not show any stress symptoms. Shoot Ni concentration was higher in N. caerulescens than in N. japonica; this difference was due to higher efficiency of root-to-shoot Ni translocation in N. caerulescens than N. japonica. It is known that the vacuole Ni transporter IREG2 suppresses Ni translocation from roots to shoots by sequestering Ni in the root vacuoles. The expression level of the IREG2 gene in the roots of N. japonica was 10-fold that in the roots of N. caerulescens. Moreover, the copy number of IREG2 per genome was higher in N. japonica than in N. caerulescens, suggesting that IREG2 expression is elevated by gene multiplication in N. japonica. The heterologous expression of IREG2 of N. japonica and N. caerulescens in yeast and A. thaliana confirmed that both IREG2 genes encode functional vacuole Ni transporters. Taking these results together, we hypothesize that the elevation of IREG2 expression by gene multiplication causes the lower root-to-shoot Ni translocation in N. japonica.
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- 2020
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8. A case report of nivolumab-induced myasthenia gravis and myositis in a metastatic renal cell carcinoma patient
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Shotaro Nakanishi, Sho Nishida, Minoru Miyazato, Masato Goya, and Seiichi Saito
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Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
We present a 78-year-old male with renal cell carcinoma who developed myasthenia gravis complicated by myositis after nivolumab administration, which was verified by the presence of antibodies against the acetylcholine receptor. The initial symptom was posterior neck pain, and biochemical examination of blood showed elevated levels of hepatic enzymes and creatine phosphokinase. The level of antibody against the acetylcholine receptor increased 4.1-fold. His condition progressed rapidly resulting in respiratory failure 15 days after conservative therapy.
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- 2020
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9. Potential Risks and Limited Indications of the Supraorbital Keyhole Approach for Clipping Internal Carotid Artery Aneurysms
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Terushige Toyooka, Kojiro Wada, Naoki Otani, Arata Tomiyama, Satoru Takeuchi, Satoshi Tomura, Sho Nishida, Hideaki Ueno, Yasuaki Nakao, Takuji Yamamoto, and Kentaro Mori
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Surgery ,RD1-811 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Background: Internal carotid artery (ICA) aneurysm may be a good target for supraorbital keyhole clipping. We discuss the surgical indications and risks of keyhole clipping for ICA aneurysms based on long-term clinical and radiologic results. Methods: This was a retrospective analysis of 51 patients (aged 35–75 years, mean 62 years) with ICA aneurysms (mean 5.8 ± 1.8 mm) who underwent clipping via the supraorbital keyhole approach between 2005 and 2017. Neurologic and cognitive functions were examined by several methods, including the modified Rankin Scale and Mini-Mental Status Examination. The state of clipping was assessed 1 year and then every few years after the operation. Results: Complete clipping was confirmed in 45 patients (88.2%), dog-ear remnants behind the clip persisted in 4 patients, and wrapping was performed in 2 patients. Mean duration of postoperative hospitalization was 3.4 ± 6.9 days. The mean clinical follow-up period was 6.6 ± 3.2 years. The overall mortality was 0, and overall morbidity (modified Rankin Scale score ≥2 or Mini-Mental Status Examination
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- 2019
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10. Roles of Non-Coding RNAs in Response to Nitrogen Availability in Plants
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Makiha Fukuda, Toru Fujiwara, and Sho Nishida
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long non-coding RNA ,microRNA ,nitrogen ,plant nutrition ,small interfering RNA ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Nitrogen (N) is an essential nutrient for plant growth and development; therefore, N deficiency is a major limiting factor in crop production. Plants have evolved mechanisms to cope with N deficiency, and the role of protein-coding genes in these mechanisms has been well studied. In the last decades, regulatory non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), small interfering RNAs (siRNAs), and long ncRNAs (lncRNAs), have emerged as important regulators of gene expression in diverse biological processes. Recent advances in technologies for transcriptome analysis have enabled identification of N-responsive ncRNAs on a genome-wide scale. Characterization of these ncRNAs is expected to improve our understanding of the gene regulatory mechanisms of N response. In this review, we highlight recent progress in identification and characterization of N-responsive ncRNAs in Arabidopsis thaliana and several other plant species including maize, rice, and Populus.
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- 2020
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11. Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds
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Yusuke Tagashira, Tomoe Shimizu, Masanobu Miyamoto, Sho Nishida, and Kaoru T. Yoshida
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ectopic expression ,mineral element ,molecular breeding ,Oryza sativa L. ,phosphorus ,phytic acid ,seed ,translocation ,Botany ,QK1-989 - Abstract
The manipulation of seed phosphorus is important for seedling growth and environmental P sustainability in agriculture. The mechanism of regulating P content in seed, however, is poorly understood. To study regulation of total P, we focused on phytic acid (inositol hexakisphosphate; InsP6) biosynthesis-related genes, as InsP6 is a major storage form of P in seeds. The rice (Oryza sativa L.) low phytic acid mutant lpa1-1 has been identified as a homolog of archael 2-phosphoglycerate kinase. The homolog might act as an inositol monophosphate kinase, which catalyzes a key step in InsP6 biosynthesis. Overexpression of the homolog in transgenic rice resulted in a significant increase in total P content in seed, due to increases in InsP6 and inorganic phosphates. On the other hand, overexpression of genes that catalyze the first and last steps of InsP6 biosynthesis could not increase total P levels. From the experiments using developing seeds, it is suggested that the activation of InsP6 biosynthesis in both very early and very late periods of seed development increases the influx of P from vegetative organs into seeds. This is the first report from a study attempting to elevate the P levels of seed through a transgenic approach.
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- 2015
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12. Induction of Nickel Accumulation in Response to Zinc Deficiency in Arabidopsis thaliana
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Sho Nishida, Aki Kato, Chisato Tsuzuki, Junko Yoshida, and Takafumi Mizuno
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Arabidopsis thaliana ,nickel ,transporter ,zinc ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Excessive accumulation of nickel (Ni) can be toxic to plants. In Arabidopsis thaliana, the Fe2+ transporter, iron (Fe)-regulated transporter1 (IRT1), mediates Fe uptake and also implicates in Ni2+ uptake at roots; however, the underlying mechanism of Ni2+ uptake and accumulation remains unelucidated. In the present study, we found that zinc (Zn) deficient conditions resulted in increased accumulation of Ni in plants, particularly in roots, in A. thaliana. In order to elucidate the underlying mechanisms of Ni uptake correlating zinc condition, we traced 63Ni isotope in response to Zn and found that (i) Zn deficiency induces short-term Ni2+ absorption and (ii) Zn2+ inhibits Ni2+ uptake, suggesting competitive uptake between Ni and Zn. Furthermore, the Zrt/Irt-like protein 3 (ZIP3)-defective mutant with an elevated Zn-deficient response exhibited higher Ni accumulation than the wild type, further supporting that the response to Zn deficiency induces Ni accumulation. Previously, expression profile study demonstrated that IRT1 expression is not inducible by Zn deficiency. In the present study, we found increased Ni accumulation in IRT1-null mutant under Zn deficiency in agar culture. These suggest that Zn deficiency induces Ni accumulation in an IRT1-independen manner. The present study revealed that Ni accumulation is inducible in response to Zn deficiency, which may be attributable to a Zn uptake transporter induced by Zn deficiency.
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- 2015
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13. Three‐dimensional vessel wall MRI to characterize thrombus prior to endovascular thrombectomy for large vessel occlusion stroke
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Kosuke Kumagai, Miyuka Hayashi, Tooru Ueda, Sho Nishida, Hideaki Ishihara, Shinji Hayashi, and Hiroshi Katoh
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Imaging, Three-Dimensional ,Humans ,Thrombosis ,Radiology, Nuclear Medicine and imaging ,Neurology (clinical) ,Magnetic Resonance Imaging ,Ischemic Stroke ,Thrombectomy - Abstract
Endovascular thrombectomy (EVT) for acute ischemic stroke (AIS) caused by large vessel occlusion requires rapid and reliable imaging of the vessel course including the clot to reduce complications and ensure success. However, no method to acquire this information has been established.Six consecutive patients with AIS caused by large vessel occlusion underwent EVT in our institute. High-resolution vessel wall MRI was performed using three-dimensional (3D) variable refocusing flip angle pulse and turbo spin-echo sequences (VRFA-TSE) with the volume isotropic turbo spin-echo acquisition (VISTA) technique. This study evaluated the effectiveness of 3D proton density-weighted (PDW) VRFA-TSE (called PDW-VISTA) compared with T2-weighted (T2W) VRFA-TSE (called T2W-VISTA) to demonstrate the cerebral vessels including the occluded invisible lesion.PDW-VISTA and T2W-VISTA were successfully performed in all cases. PDW-VISTA was more useful to visualize the anterior circulation than T2W-VISTA by clearly revealing invisible vessels, whereas PDW-VISTA and T2W-VISTA had similar findings in the posterior circulation. The vessel courses shown by 3D PDW-VISTA imaging before treatment and digital subtraction angiography after treatment showed good agreement in all cases. Furthermore, 3D PDW-VISTA imaging demonstrated the length and size of the clot.PDW-VISTA imaging was found to more clearly depict the cerebral vessels including occluded lesion than T2W-VISTA imaging. Findings of thrombus size and length were important for correctly placing the stent retriever and securing safety during the procedure.
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- 2022
14. Retroperitoneoscopic Donor Nephrectomy on the Right Side Provides Equivalent Outcomes and Donor Safety to That on the Left Side
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Sho Nishida, Takehiro Ohyama, Kyoko Minamisono, Hajime Sasaki, and Daiki Iwami
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Transplantation ,Surgery - Published
- 2023
15. Ethylene works as a possible regulator for the rootlet elongation and transcription of genes for phosphorus acquisition in cluster roots of Lupinus albus L
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Hirotsuna Yamada, Sho Nishida, and Jun Wasaki
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Soil Science ,Plant Science - Published
- 2022
16. Glucosinolate catabolism maintains glucosinolate profiles and transport in sulfur-starvedArabidopsis
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Liu Zhang, Ryota Kawaguchi, Takuro Enomoto, Sho Nishida, Meike Burow, and Akiko Maruyama-Nakashita
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Glucosinolates (GSL) are sulfur (S)-rich specialized metabolites produced by plants of the Brassicales order. Our previous study found that in Arabidopsis seedlings, S deficiency (−S) promoted GSL catabolism by activating two ß-glucosidases (BGLU), BGLU28 and BGLU30. The induced GSL catabolism was a survival strategy for seedlings grown under −S, because S released from GSL was reincorporated into primary S metabolites which are essential for plant growth. However, as GSL profile in plants vary among growth stages and organs, we set out to test a potential contribution of BGLU28/30-dependent GSL catabolism at the reproductive growth stage. Thus, in this study, we assessed growth, metabolic, and transcriptional phenotypes of maturebglu28/30double mutants grown under different S conditions. Our results showed that compared to wild-type plants grown under −S, maturebglu28/30mutants displayed impaired growth and accumulated increased levels of GSL in their mature seeds, siliques, flowers, and rosette leaves of before bolting plants. In contrast, the levels of primary S-containing metabolites, glutathione and cysteine, were decreased in mature seeds. Furthermore, the transport of GSL from rosette leaves to the reproductive organs was stimulated in thebglu28/30mutants under −S. Transcriptome analysis revealed that genes related to other biological processes, such as phytohormone signaling and plant response to heat, responded differentially to −S in thebglu28/30mutants. Altogether, these findings broadened our understanding of the roles of BGLU28/30-dependent GSL catabolism in plant adaptation to nutrient stress.One-sentence summaryDisruption of glucosinolate catabolic genes,BGLU28andBGLU30, in sulfur-starved matureArabidopsisimpaired growth, affected glucosinolate distribution, and altered transcriptional profiles.
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- 2022
17. Retargetable Netlists Generation and Structural Synthesis Based on a Meta Hardware Description Language: Melasy+.
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Sho Nishida and Katsumi Wasaki
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- 2012
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18. Creatinine reduction ratio on postoperative day 2 predicts long‐term outcomes after living donor kidney transplantation
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Saki Katano, Yoshitaka Kinoshita, Toshihiro Shimizu, Sho Nishida, Daiki Iwami, Haruki Kume, and Tetsuya Fujimura
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Graft Rejection ,Multivariate statistics ,medicine.medical_specialty ,Multivariate analysis ,business.industry ,Urology ,medicine.medical_treatment ,Incidence (epidemiology) ,Graft Survival ,Renal function ,medicine.disease ,Kidney Transplantation ,Treatment Outcome ,surgical procedures, operative ,Creatinine ,Bayesian multivariate linear regression ,ABO blood group system ,Living Donors ,medicine ,Humans ,business ,Dialysis ,Kidney transplantation ,Retrospective Studies - Abstract
OBJECTIVES To evaluate the relationship between the creatinine reduction ratio between postoperative days 1 and 2 and post-transplantation clinical outcomes after living donor kidney transplantation. METHODS Clinical data of patients who underwent living donor kidney transplantation at Jichi Medical University Hospital, Tochigi, Japan, between 2006 and 2019 were retrieved. The creatinine reduction ratio between postoperative days 1 and 2 was calculated based on the formula: (Cre1 - Cre2) × 100/Cre1; patients were then classified into either the slow graft function (creatinine reduction ratio between postoperative days 1 and 2 ≤30%) or immediate graft function (creatinine reduction ratio between postoperative days 1 and 2 >30%) group. We carried out the log-rank test and multivariate Cox proportional hazards regression analyses to assess graft survival and rejection-free survival, and the unpaired t-test and multivariate linear regression to assess post-transplantation estimated glomerular filtration rates. Multivariate analyses used age, sex, dialysis duration, ABO compatibility, donor-specific antibody positivity and medically complex living donors as explanatory variables. RESULTS Of the 272 patients, 30 and 242 were in the slow graft function and immediate graft function groups, respectively. Multivariate Cox proportional hazards regression analyses showed a significantly higher incidence of overall and death-censored graft loss in the slow graft function group than the immediate graft function group. The frequency of rejection after 1 week post-transplantation did not differ within the groups. Post-transplantation estimated glomerular filtration rates tended to decline earlier in the slow graft function group than in the immediate graft function group; however, the difference was not statistically significant. CONCLUSIONS The creatinine reduction ratio between postoperative days 1 and 2 could potentially predict long-term outcomes after living donor kidney transplantation. Using the creatinine reduction ratio between postoperative days 1 and 2 and other conventional indicators might allow accurate risk classification and appropriate therapeutic interventions.
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- 2021
19. Exploration of unknown nickel-containing proteins from plants by liquid chromatography–inductively coupled plasma mass spectrometry
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Lumi Negishi, Naoki Furuta, Aimi Takahashi, Sho Nishida, and Michio Suzuki
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0106 biological sciences ,chemistry.chemical_classification ,Chromatography ,Urease ,biology ,Soil Science ,chemistry.chemical_element ,04 agricultural and veterinary sciences ,Plant Science ,biology.organism_classification ,01 natural sciences ,Cofactor ,Metal ,Nickel ,Enzyme ,chemistry ,Liquid chromatography–mass spectrometry ,visual_art ,040103 agronomy & agriculture ,biology.protein ,visual_art.visual_art_medium ,0401 agriculture, forestry, and fisheries ,Arabidopsis thaliana ,Inductively coupled plasma mass spectrometry ,010606 plant biology & botany - Abstract
Nickel (Ni) is an essential cofactor for various microbial enzymes, but in higher plants, few Ni-containing enzymes (e.g., urease) have been identified. Here, we explored the use of liquid chromato...
- Published
- 2020
20. Aberrant RNA splicing of the phytic acid synthesis gene inositol-1,3,4 trisphosphate 5/6-kinase in a low phytic acid soybean line
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Dong Qin, Sho Nishida, Rumi Tominaga, Akihiro Ueda, Victor Raboy, and Hirofumi Saneoka
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Soil Science ,Plant Science - Abstract
Most phosphorus is stored as phytic acid in plant seed, although it is considered an anti-nutrient compound that reduces the bioavailability of minerals in monogastric animals. Low-phytic acid (LP) crops improve mineral bioavailability in monogastric animals, which can decrease environmental pollution due to phosphorus. Recently, we reported a newly developed LP soybean line by crossing the LP line CX1834 and Japanese normal phytic acid cultivar Natto-kotsubu (Natto), which showed superior traits in seedling emergence, growth, and yield compared with CX1834. The molecular mechanism underlying the LP trait in the LP line remains unclear. The LP line was used for a de novo RNA-Seq analysis, and we illustrated the aberrant RNA splicing of the phytic acid synthase gene inositol-1,3,4 trisphosphate 5/6-kinase 3 (GmITPK3) in the LP line. The expression level of phytic acid biosynthesis-related genes and inositol phosphate isomer concentrations were investigated. A de novo RNA-Seq analysis revealed that the intron retention (IR) transcript of GmITPK3 is highly expressed in the leaves of the LP line. Although expressed in both, the relative abundances of multiple IR transcripts were significantly higher in the LP line than that in the Natto cultivar. The IR transcripts of GmITPK3 encoding truncated proteins were missing an essential domain for their activity, while one possibly contained an abnormal ligand binding site. The relative abundance of IR transcripts was 1.9- to 5.4-times higher in the LP line than that in the Natto cultivar during seed development; accordingly, the production of phytic acid and its precursors in the developing seeds was lower in the LP line than that in the Natto cultivar. No mutation was detected at the exon–intron junction, where a splicing error occurs in GmITPK3 of the LP line, implying that a splicing error is attributed to unknown splicing factor genes involved in the splicing regulation of GmITPK3. Our results provide insights into the possible involvement of splicing errors in GmITPK3 for the trait of low phytic acid production in the LP line.
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- 2022
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21. Local Application of Magnesium Sulfate Solution Suppressed Cortical Spreading Ischemia and Reduced Brain Damage in a Rat Subarachnoid Hemorrhage-Mimicking Model
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Shunichi Sato, Kojiro Wada, Satoru Takeuchi, Satoko Kawauchi, Akihide Kondo, Kosuke Kumagai, Kentaro Mori, Sho Nishida, and Terushige Toyooka
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Male ,medicine.medical_specialty ,Subarachnoid hemorrhage ,Ischemia ,chemistry.chemical_element ,Brain damage ,Brain Ischemia ,Brain ischemia ,Rats, Sprague-Dawley ,Magnesium Sulfate ,Internal medicine ,medicine ,Animals ,Analgesics ,biology ,business.industry ,Magnesium ,Cortical Spreading Depression ,Depolarization ,Subarachnoid Hemorrhage ,medicine.disease ,Rats ,Nitric oxide synthase ,Disease Models, Animal ,Pharmaceutical Solutions ,Endocrinology ,Cerebral blood flow ,chemistry ,Cerebrovascular Circulation ,biology.protein ,Surgery ,Neurology (clinical) ,medicine.symptom ,business - Abstract
Objective Cortical spreading depolarization (CSD), cortical spreading ischemia (CSI), and early brain injury are involved in the occurrence of delayed brain ischemia after subarachnoid hemorrhage (SAH). We tested whether local application of magnesium (Mg) sulfate solution suppressed CSD and CSI, and decreased brain damage in a rat SAH-mimicking model. Methods Nitric oxide synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME) and high concentration potassium solution were topically applied to simulate the environment after SAH. We irrigated the parietal cortex with artificial cerebrospinal fluid (ACSF), containing L-NAME (1 mM), K+ (35 mM), and Mg2+ (5 mM). Forty-five rats were divided into 3 groups: sham surgery (sham group), L-NAME + [K+]ACSF (control group), and L-NAME + [K+]ACSF + [Mg2+] (Mg group). CSD was induced by topical application with 1 M KCl solution in 3 groups. The effects of Mg administration on CSD and cerebral blood flow were evaluated. Histological brain tissue damage, body weight, and neurological score were assessed at 2 days after insult. Results Mg solution significantly shortened the total depolarization time, and reduced CSI, histological brain damage, and brain edema compared with those of the control group (P Conclusions Local application of Mg suppressed CSI and reduced brain damage in a rat SAH-mimicking model. Mg irrigation therapy may be beneficial to suppress brain damage due to CSI after SAH.
- Published
- 2021
22. Differences in mineral accumulation and gene expression profiles between two metal hyperaccumulators, Noccaea japonica and Noccaea caerulescens ecotype Ganges, under excess nickel condition
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Takuo Enomoto, Junko Yoshida, Sho Nishida, Takafumi Mizuno, and Toshihiro Watanabe
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Minerals ,biology ,Ecotype ,Short Communication ,Arabidopsis ,food and beverages ,chemistry.chemical_element ,Plant Science ,biology.organism_classification ,Japonica ,Metal ,Nickel ,chemistry ,visual_art ,Botany ,Gene expression ,Soil water ,Brassicaceae ,visual_art.visual_art_medium ,Arabidopsis thaliana ,Hyperaccumulator ,Transcriptome - Abstract
Here we compare mineral accumulation and global gene expression patterns between two metal hyperaccumulator plants - Noccaea japonica, originating from Ni-rich serpentine soils, and Noccaea caerulescens (ecotype Ganges), originating from Zn/Pb-mine soils - under excess Ni conditions. Significant differences in the accumulation of K, P, Mg, B, and Mo were explained by the expression levels of specific transporters for each mineral. We previously showed that total Ni accumulation in the whole plant is higher in N. caerulescens than in N. japonica. Here we found a similar tendency for Fe under excess Ni; however, the expression of iron-regulated transporter 1 (IRT1), which encodes the primary Fe uptake transporter and causes excess Ni uptake in Arabidopsis thaliana, was higher in N. japonica. NjIRT1 has a point mutation at Asp100, which is essential for Fe transport, and so might lack its Fe and possibly Ni transport function. Noccaea japonica might have lost its IRT1 function, which would prevent excess Ni uptake via IRT1 in Ni-rich soils, and come to rely on other transporters.
- Published
- 2021
23. Evaluation of measurement uncertainty in the elemental analysis of sintered silicon carbide using laser ablation in liquid—inductively coupled plasma mass spectrometry with external calibration and isotope dilution
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Naoki Furuta, Naoko Nonose, Sho Nishida, Masahide Fujiwara, and Koki Hirosawa
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Materials science ,Laser ablation ,Isotope ,General Chemical Engineering ,010401 analytical chemistry ,Analytical chemistry ,General Chemistry ,Isotope dilution ,Mass spectrometry ,01 natural sciences ,0104 chemical sciences ,010309 optics ,chemistry.chemical_compound ,chemistry ,Elemental analysis ,0103 physical sciences ,Silicon carbide ,Measurement uncertainty ,Safety, Risk, Reliability and Quality ,Instrumentation ,Inductively coupled plasma mass spectrometry - Abstract
The goal of this study was to evaluate the uncertainty of elemental analytical methods that use laser ablation in liquid (LAL) as a pretreatment. After LAL sampling of silicon carbide (SiC), trace impurities were quantified using inductively coupled plasma-sector field mass spectrometry (ICP-SFMS) with external calibration (EC). The expanded uncertainty (k = 2) of the concentrations was less than 10 %. To obtain more precise values, the Ti, the element homogeneously distributed on the sample surface of SiC, was quantified using ICP-SFMS with isotope dilution mass spectrometry (IDMS). The expanded uncertainty (k = 2) was reduced to 3.4 %. The smaller uncertainty associated with IDMS reflected the fact that measuring the isotope ratio of the same element with IDMS and high-speed isotope measurements at 10-ms intervals reduced the variability of signal intensities, the primary source of uncertainty, more effectively than EC. Moreover, the combination with ID improved the sample amount-dependent unrepeatability in pretreatment.
- Published
- 2019
24. Long-term impact of baseline serum uric acid levels on living kidney donors: a retrospective study
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Akari Kaba, Mariko Toyoda, Hiroshi Yokomizo, Sho Nishida, Shigeyoshi Yamanaga, Toshinori Ishizuka, Yuji Hidaka, Kohei Kinoshita, Akira Miyata, Masayuki Kashima, Chiaki Kawabata, Satoshi Hamanoue, Kenji Okumura, and Kosuke Tanaka
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Nephrology ,Male ,medicine.medical_specialty ,Time Factors ,Renal function ,lcsh:RC870-923 ,Gastroenterology ,Donor Selection ,chemistry.chemical_compound ,Serum uric acid ,Living kidney donors ,Diabetes mellitus ,Internal medicine ,medicine ,Living Donors ,Humans ,Adverse effect ,Aged ,Retrospective Studies ,business.industry ,Donor selection ,Middle Aged ,lcsh:Diseases of the genitourinary system. Urology ,medicine.disease ,Kidney Transplantation ,Gout ,Uric Acid ,Treatment Outcome ,chemistry ,Adverse events ,Preoperative Period ,Uric acid ,Female ,business ,Dyslipidemia ,Research Article - Abstract
BackgroundPreoperative characteristics of living kidney donors are commonly considered during donor selection and postoperative follow-up. However, the impact of preoperative uric acid (UA) levels is poorly documented. The aim of this study was to evaluate the association between preoperative serum UA levels and post-donation long-term events and renal function.MethodsThis was a single-center retrospective analysis of 183 living kidney donors. The donors were divided into high (≥5.5 mg/dl) and low (ResultsIn total, 44 donors experienced 52 adverse events over 5 years. The incidence of adverse events within 5 years was significantly higher in the high UA group than in the low UA group (50% vs. 24%,p = 0.003); this was true even after the exclusion of hyperuricemia-related events (p = 0.047). UA emerged as an independent risk factor for adverse events (p = 0.012). Donors with higher UA levels had lower eGFRs after donation, whereas body mass index, hemoglobin A1c, blood pressure, and low-density lipoprotein cholesterol did not have any impact on the eGFR.ConclusionsThe findings suggest that preoperative UA levels should be considered during donor selection and postoperative follow-up.
- Published
- 2021
25. Factors related to suboptimal recovery of renal function after living donor nephrectomy: a retrospective study
- Author
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Chiaki Kawabata, Asami Takeda, Satoshi Hamanoue, Akito Inadome, Mariko Toyoda, Kosuke Tanaka, Hiroshi Yokomizo, Shigeyoshi Yamanaga, Soichi Uekihara, Kohei Kinoshita, Yuji Hidaka, and Sho Nishida
- Subjects
Male ,Nephrology ,medicine.medical_specialty ,030232 urology & nephrology ,Urology ,Renal function ,030204 cardiovascular system & hematology ,lcsh:RC870-923 ,Kidney ,Nephrectomy ,Muscle hypertrophy ,03 medical and health sciences ,chemistry.chemical_compound ,Postoperative Complications ,0302 clinical medicine ,Internal medicine ,Biopsy ,Living Donors ,medicine ,Humans ,Urea ,Kidney transplant ,Retrospective Studies ,Glycated Hemoglobin ,medicine.diagnostic_test ,Living donor ,business.industry ,Age Factors ,Hypertrophy ,Recovery of Function ,Odds ratio ,Middle Aged ,lcsh:Diseases of the genitourinary system. Urology ,Confidence interval ,Uric Acid ,medicine.anatomical_structure ,chemistry ,Chronic Disease ,Kidney Failure, Chronic ,Uric acid ,Female ,business ,Glomerular Filtration Rate ,Research Article - Abstract
Background The renal function of the remaining kidney in living donors recovers up to 60~70% of pre-donation estimated-glomerular filtration rate (eGFR) by compensatory hypertrophy. However, the degree of this hypertrophy varies from donor to donor and the factors related to it are scarcely known. Methods We analyzed 103 living renal transplantations in our institution and divided them into two groups: compensatory hypertrophy group [optimal group, 1-year eGFR ≥60% of pre-donation, n = 63] and suboptimal compensatory hypertrophy group (suboptimal group, 1-year eGFR n = 40). We retrospectively analyzed the factors related to suboptimal compensatory hypertrophy. Results Baseline eGFRs were the same in the two groups (optimal versus suboptimal: 82.0 ± 13.1 ml/min/1.73m2 versus 83.5 ± 14.8 ml/min/1.73m2, p = 0.588). Donor age (optimal versus suboptimal: 56.0 ± 10.4 years old versus 60.7 ± 8.7 years old, p = 0.018) and uric acid (optimal versus suboptimal: 4.8 ± 1.2 mg/dl versus 5.5 ± 1.3 mg/dl, p = 0.007) were significantly higher in the suboptimal group. The rate of pathological chronicity finding on 1-h biopsy (ah≧1 ∩ ct + ci≧1) was much higher in the suboptimal group (optimal versus suboptimal: 6.4% versus 25.0%, p = 0.007). After the multivariate analysis, the pathological chronicity finding [odds ratio (OR): 4.8, 95% confidence interval (CI): 1.3–17.8, p = 0.021] and uric acid (per 1.0 mg/dl, OR: 1.5, 95% CI: 1.1–2.2, p = 0.022) were found to be independent risk factors for suboptimal compensatory hypertrophy. Conclusion Chronicity findings on baseline biopsy and higher uric acid were associated with insufficient recovery of the post-donated renal function.
- Published
- 2019
26. Monitoring of iodine species during water purification at a public water treatment plant in Japan
- Author
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Horigome Jun, Naoki Furuta, Naoya Ohata, Maki Asami, and Sho Nishida
- Subjects
chemistry.chemical_classification ,0208 environmental biotechnology ,Iodide ,Fluorescence spectrometry ,chemistry.chemical_element ,Portable water purification ,02 engineering and technology ,010501 environmental sciences ,Iodine ,01 natural sciences ,020801 environmental engineering ,chemistry.chemical_compound ,chemistry ,Environmental chemistry ,Chlorine ,Water treatment ,Raw water ,Iodate ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
Iodine is an essential element for humans; however, it can be toxic depending on its chemical form. A variety of toxic and non-toxic iodine species have been identified in environmental water and in the drinking water produced by public water treatment plants. Here, we examined the change of iodine species during the water treatment process at a public water treatment plant in Japan. Samples of raw water and of treated water immediately after each of eight treatment stages comprising the treatment process were collected, and a speciation analysis was conducted by means of ion chromatography– and size-exclusion chromatography–inductively coupled plasma mass spectrometry. In the raw water, iodine was found mainly as iodide or iodinated humic substances that were ultimately oxidized and transformed into iodate, a form non-toxic to humans, by two independent oxidation stages in the water treatment process – ozone treatment and chlorine treatment. No disinfection byproducts were detected at any stage of the treatment process. Fluorescence spectrometry with multivariate analysis revealed that humic substances were markedly decreased by ozone treatment, but not by chlorine treatment. The present results show that, at the plant-scale, ozone treatment is an effective means of removing toxic iodine species from raw water.
- Published
- 2018
27. Organ-specific allocation pattern of acquired phosphorus and dry matter in two rice genotypes with contrasting tolerance to phosphorus deficiency
- Author
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Keitaro Tawaraya, Jun Wasaki, Sho Nishida, and D. M. S. B. Dissanayaka
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0106 biological sciences ,0301 basic medicine ,Regosol ,Phosphorus ,Soil Science ,chemistry.chemical_element ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,Horticulture ,030104 developmental biology ,Dry weight ,chemistry ,Soil horizon ,Phosphorus deficiency ,Dry matter ,Cultivar ,010606 plant biology & botany ,Panicle - Abstract
Our earlier study demonstrated that the landrace of Japonica rice, Akamai exhibits low-P (phosphorous) tolerance mechanisms compared to the conventional type cultivar, Koshihikari. The present study examined the genotypic difference of yield, plasticity of root growth, and internal utilization of acquired P (allocation pattern of biomass and P among different vegetative and reproductive organs) of two contrasting cultivars in response to P-deficiency. Each cultivar was grown until maturity with (+P) and without (–P) P supply in pots (two plants per pot) filled with 15 kg of Regosol soil. Grain yield and yield components were determined along with biomass and P accumulation in different vegetative and reproductive organs. To assess the plasticity of root growth, the soil column in the pot was divided into two equal portions (upper and lower soil layers) in which the root dry weight and length were measured separately. Among the investigated yield components, the number of filled grains per panicle ...
- Published
- 2018
28. Identification of genomic regions associated with low phosphorus tolerance in japonica rice (Oryza sativa L.) by QTL-Seq
- Author
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Yoshiko Tateishi, Hayato Maruyama, Masaru Chuba, Soichiro Honda, Sho Nishida, D. M. S. B. Dissanayaka, Jun Wasaki, and Keitaro Tawaraya
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0106 biological sciences ,0301 basic medicine ,Oryza sativa ,Phosphorus ,food and beverages ,Soil Science ,chemistry.chemical_element ,Plant Science ,engineering.material ,Quantitative trait locus ,Biology ,01 natural sciences ,Japonica rice ,03 medical and health sciences ,030104 developmental biology ,Nutrient ,Agronomy ,chemistry ,engineering ,Fertilizer ,010606 plant biology & botany - Abstract
Phosphorus (P) is a major nutrient supporting rice productivity. Improving low-P tolerance of rice is expected to reduce dependence on P fertilizer, thereby reducing rice production costs and envir...
- Published
- 2017
29. Landrace of japonica rice, Akamai exhibits enhanced root growth and efficient leaf phosphorus remobilization in response to limited phosphorus availability
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Sho Nishida, Jun Wasaki, Keitaro Tawaraya, Hayato Maruyama, and D. M. S. B. Dissanayaka
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0106 biological sciences ,biology ,Phosphorus ,Soil Science ,chemistry.chemical_element ,04 agricultural and veterinary sciences ,Plant Science ,biology.organism_classification ,01 natural sciences ,Acclimatization ,Crop ,Japanese rice ,Agronomy ,chemistry ,Dry weight ,Shoot ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Cultivar ,010606 plant biology & botany ,Panicle - Abstract
Phosphorus (P) acquisition through extensive root growth and P allocation to different plant organs through efficient remobilization are important for acclimation of crop plants to P-limited environments. This study elucidated changes in rice root growth and leaf P-remobilization and their influence on grain yield under P deficiency. Two pot experiments were conducted with (P100) and without (P0) inorganic P supply using two Japanese rice cultivars: Akamai (Yamagata) and Koshihikari. Multiple harvests were made until the panicle initiation stage. Root and shoot growth response, P acquisition, and temporal leaf P-remobilization efficiency were measured. A separate experiment ascertained the final yield and grain P status. The Akamai rice cultivar showed enhanced root growth and more acquired soil P. The Akamai root dry weight was 66% greater than that of Koshihikari under P0. Confronting P deficiency, Akamai remobilized some P from its lower mature leaves to upper younger leaves starting from early growth. The remobilized P fraction increased to 72% at panicle initiation under P0. Under P0, Akamai exhibited two-fold higher leaf P-remobilization efficiency than under P100. Enhanced root growth that facilitates acquisition of more soil P through better soil exploration coupled with efficient leaf P remobilization from the early growth stage improves adaptation of Akamai rice cultivar to P-limited environments. Nevertheless, P-starvation responses did not facilitate higher grain yields in P-limited conditions.
- Published
- 2016
30. Genome-Wide Analysis of Long Intergenic Noncoding RNAs Responding to Low-Nutrient Conditions in Arabidopsis thaliana: Possible Involvement of Trans-Acting siRNA3 in Response to Low Nitrogen
- Author
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Toru Fujiwara, Yukihisa Shimada, Makiha Fukuda, Yusuke Kakei, and Sho Nishida
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0106 biological sciences ,0301 basic medicine ,Small interfering RNA ,Physiology ,Nitrogen ,Arabidopsis ,Plant Science ,Biology ,01 natural sciences ,Transcriptome ,03 medical and health sciences ,Intergenic region ,Arabidopsis thaliana ,RNA, Small Interfering ,Genetics ,Regulation of gene expression ,RNA ,Computational Biology ,Cell Biology ,General Medicine ,Nutrients ,biology.organism_classification ,Long non-coding RNA ,030104 developmental biology ,RNA, Plant ,Trans-Activators ,RNA, Long Noncoding ,Trans-acting ,Genome, Plant ,010606 plant biology & botany ,Signal Transduction - Abstract
Long intergenic noncoding RNAs (lincRNAs) play critical roles in transcriptional and post-transcriptional regulation of gene expression in a wide variety of organisms. Thousands of lincRNAs have been identified in plant genomes, although their functions remain mostly uncharacterized. Here, we report a genome-wide survey of lincRNAs involved in the response to low-nutrient conditions in Arabidopsis thaliana. We used RNA sequencing data derived from A. thaliana roots exposed to low levels of 12 different nutrients. Using bioinformatics approaches, 60 differentially expressed lincRNAs were identified that were significantly upregulated or downregulated under deficiency of at least one nutrient. To clarify their roles in nutrient response, correlations of expression patterns between lincRNAs and reference genes were examined across the 13 conditions (12 low-nutrient conditions and control). This analysis allowed us to identify lincRNA-RNA pairs with highly positive or negative correlations. In addition, calculating interaction energies of those pairs showed lincRNAs that may act as regulatory interactors; e.g. small interfering RNAs (siRNAs). Among them, trans-acting siRNA3 (TAS3), which is known to promote lateral root development by producing siRNA against Auxin response factor 2, 3, and 4, was revealed as a nitrogen (N)-responsive lincRNA. Furthermore, nitrate transporter 2 was identified as a potential target of TAS3-derived siRNA, suggesting that TAS3 participates in multiple pathways by regulating N transport and root development under low-N conditions. This study provides the first resource for candidate lincRNAs involved in multiple nutrient responses in plants.
- Published
- 2018
31. Elevated expression of vacuolar nickel transporter gene IREG2 is associated with reduced root-to-shoot nickel translocation in Noccaea japonica
- Author
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Sho, NISHIDA, Ryoji, TANIKAWA, Shota, ISHIDA, Junko, YOSHIDA, Takafumi, MIZUNO, nHiromi, NAKANISHI, and Naoki, FURUTA\n
- Abstract
application/pdf
- Published
- 2021
32. Roles of Non-Coding RNAs in Response to Nitrogen Availability in Plants
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Toru Fujiwara, Makiha Fukuda, and Sho Nishida
- Subjects
0106 biological sciences ,0301 basic medicine ,Small interfering RNA ,RNA, Untranslated ,Nitrogen ,Arabidopsis ,plant nutrition ,Review ,Computational biology ,01 natural sciences ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,Transcriptome ,03 medical and health sciences ,Gene Expression Regulation, Plant ,microRNA ,Gene expression ,Arabidopsis thaliana ,Physical and Theoretical Chemistry ,lcsh:QH301-705.5 ,Molecular Biology ,Gene ,Spectroscopy ,long non-coding RNA ,biology ,Gene Expression Profiling ,fungi ,Organic Chemistry ,food and beverages ,General Medicine ,biology.organism_classification ,small interfering RNA ,Long non-coding RNA ,Computer Science Applications ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,RNA, Plant ,Identification (biology) ,010606 plant biology & botany - Abstract
Nitrogen (N) is an essential nutrient for plant growth and development; therefore, N deficiency is a major limiting factor in crop production. Plants have evolved mechanisms to cope with N deficiency, and the role of protein-coding genes in these mechanisms has been well studied. In the last decades, regulatory non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), small interfering RNAs (siRNAs), and long ncRNAs (lncRNAs), have emerged as important regulators of gene expression in diverse biological processes. Recent advances in technologies for transcriptome analysis have enabled identification of N-responsive ncRNAs on a genome-wide scale. Characterization of these ncRNAs is expected to improve our understanding of the gene regulatory mechanisms of N response. In this review, we highlight recent progress in identification and characterization of N-responsive ncRNAs in Arabidopsis thaliana and several other plant species including maize, rice, and Populus.
- Published
- 2020
33. Enhanced arsenic sensitivity with excess phytochelatin accumulation in shoots of a SULTR1;2 knockout mutant of Arabidopsis thaliana (L.) Heynh
- Author
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Toru Fujiwara, Shimpei Uraguchi, Gui-Lan Duan, Sho Nishida, and Naoko Ohkama-Ohtsu
- Subjects
inorganic chemicals ,0106 biological sciences ,0301 basic medicine ,Mutant ,Sulfur metabolism ,Soil Science ,chemistry.chemical_element ,Plant Science ,Glutathione ,Biology ,biology.organism_classification ,01 natural sciences ,Sulfur ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Biochemistry ,Shoot ,Arabidopsis thaliana ,Phytochelatin ,Arsenic ,010606 plant biology & botany - Abstract
One mechanism of arsenic detoxification in plants is synthesis of phytochelatins from glutathione in a sulfur-dependent manner. This study examined the contribution of a sulfate transporter, SULTR1;2, in arsenic tolerance in terms of sulfur metabolism in Arabidopsis thaliana (L.) Heynh. Comparative analysis of SULTR mutants showed that defective mutations of SULTR1;2 resulted in an increased arsenic sensitivity, in both shoots and roots, establishing that SULTR1;2 is required for arsenic tolerance. We subsequently quantified total sulfur content and levels of sulfur compounds (phytochelatins, glutathione, cysteine) in a SULTR1;2 mutant, sel1-8. Arsenic treatments increased sulfur uptake of sel1-8, but the mutant was unable to maintain the proper basal level of sulfur. Despite drastic reduction of total sulfur content, the mutant accumulated substantial or rather excess phytochelatins in shoots under arsenic stress conditions. The levels of glutathione and cysteine in shoots were lower in sel1-8 th...
- Published
- 2016
34. Large-scale profiling of brown rice ionome in an ethyl methanesulphonate-mutagenized hitomebore population and identification of high- and low-cadmium lines
- Author
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Sho Nishida, Toru Fujiwara, Takehiro Kamiya, and Nobuhiro Tanaka
- Subjects
0106 biological sciences ,0301 basic medicine ,Cadmium ,education.field_of_study ,Mutant ,Population ,Wild type ,food and beverages ,Soil Science ,chemistry.chemical_element ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,Horticulture ,030104 developmental biology ,Agronomy ,chemistry ,Shoot ,Brown rice ,Cultivar ,education ,Ionomics ,010606 plant biology & botany - Abstract
Improving the nutritional value of cereal grains and reducing the intake of toxic elements, such as cadmium (Cd), from grains contribute to food quality. Here, we report a large-scale profiling of the brown rice ionome in an ethyl methanesulphonate (EMS)-mutagenized population of the commercial rice cultivar ‘Hitomebore’. Broad-sense heritability for manganese, zinc, and cesium were greater than 0.5, suggesting that the EMS population is suitable for screening mutants with different concentrations of these elements. From the EMS-mutagenized population, we isolated two mutant lines with high and low Cd levels. Sequence analysis revealed that the high-Cd line had a mutation at position 105 (leucine to histidine) in OsHMA3, a gene that regulates Cd accumulation in rice shoots. The low-Cd line had a mutation at position 242 (histidine to glutamine) in OsNramp5, a gene responsible for Cd uptake. Agronomical traits of the low-Cd line were indistinguishable from those of the wild type, indicating that the low-Cd line which we isolated is applicable to Hitomebore farming areas. The present study shows the effectiveness of mutant lines derived from elite cultivars in developing agriculturally useful lines with different mineral contents.
- Published
- 2016
35. Surgical Strategies and Technical Tips for Performing Extracranial-Intracranial Bypass for Occlusive Cerebrovascular Disease
- Author
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Terushige Toyooka, Kojiro Wada, Sho Nishida, Yasufumi Inaka, Kohsuke Kumagai, Naoki Otani, Kentaro Mori, Shunsuke Tanoue, and Kazuya Fujii
- Subjects
03 medical and health sciences ,medicine.medical_specialty ,0302 clinical medicine ,Extracranial intracranial bypass ,business.industry ,Occlusive ,medicine ,business ,030217 neurology & neurosurgery ,030218 nuclear medicine & medical imaging ,Surgery - Published
- 2016
36. HbA1c and Aortic Calcification Index as Noninvasive Predictors of Pre-Existing Histopathological Damages in Living Donor Kidney Transplantation
- Author
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Shigeyoshi Yamanaga, Hiroshi Yokomizo, Satoshi Hamanoue, Kohei Kinoshita, Yuji Hidaka, Mariko Toyoda, Kosuke Tanaka, Akari Kaba, Toshinori Ishizuka, Sho Nishida, Asami Takeda, Masayuki Kashima, Chiaki Kawabata, Akira Miyata, and Kenji Okumura
- Subjects
medicine.medical_specialty ,HbA1c ,030232 urology & nephrology ,Urology ,lcsh:Medicine ,Renal function ,Aortic calcification ,030230 surgery ,Living donor ,Article ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Kidney transplantation ,Kidney ,business.industry ,lcsh:R ,Significant difference ,aortic calcification index ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Blood pressure ,renal function recovery ,Hemoglobin ,business ,renal aging - Abstract
We previously reported that allografts from living donors may have pre-existing histopathological damages, defined as the combination of interstitial fibrosis (ci), tubular atrophy (ct), and arteriolar hyalinosis (ah) scores of ≧1, according to the Banff classification. We examined preoperative characteristics to identify whether the degree of these damages was related to metabolic syndrome-related factors of donors. We conducted a single-center cross-sectional analysis including 183 living kidney donors. Donors were divided into two groups: chronic change (ci + ct ≧ 1 &cap, ah ≧ 1, n = 27) and control (n = 156). Preoperative characteristics, including age, sex, blood pressure, hemoglobin A1c (HbA1c), aortic calcification index (ACI), and psoas muscle index (PMI), were analyzed. Comparing the groups, the baseline estimated glomerular filtration rate was not significantly different, however, we observed a significant difference for ACI (p = 0.009). HbA1c (p = 0.016) and ACI (p = 0.006) were independent risk factors to predict pre-existing histopathological damages, whereas PMI was not. HbA1c correlated with ct scores (p = 0.035), and ACI correlated with ci (p = 0.005), ct (p = 0.021), and ah (p = 0.017). HbA1c and ACI may serve as preoperative markers for identifying pre-existing damages on the kidneys of living donors.
- Published
- 2020
37. A case report of nivolumab-induced myasthenia gravis and myositis in a metastatic renal cell carcinoma patient
- Author
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Seiichi Saito, Shotaro Nakanishi, Minoru Miyazato, Masato Goya, and Sho Nishida
- Subjects
medicine.medical_specialty ,PUL, pulmonale ,Urology ,PLE, pleura ,030232 urology & nephrology ,lcsh:RC870-923 ,AChR, acetylcholine receptor ,Gastroenterology ,CPK, creatine phosphokinase ,MG, myasthenia gravis ,03 medical and health sciences ,IMDC, International Metastatic RCC Database Consortium ,0302 clinical medicine ,Renal cell carcinoma ,Internal medicine ,Medicine ,irAE, immune-related adverse events ,Myositis ,Acetylcholine receptor ,biology ,business.industry ,PD-1, programmed cell death-1 ,lcsh:Diseases of the genitourinary system. Urology ,medicine.disease ,Myasthenia gravis ,Oncology ,Respiratory failure ,ICI, immune checkpoint inhibitors ,030220 oncology & carcinogenesis ,biology.protein ,Creatine kinase ,RCC, renal cell carcinoma ,Nivolumab ,Antibody ,business - Abstract
We present a 78-year-old male with renal cell carcinoma who developed myasthenia gravis complicated by myositis after nivolumab administration, which was verified by the presence of antibodies against the acetylcholine receptor. The initial symptom was posterior neck pain, and biochemical examination of blood showed elevated levels of hepatic enzymes and creatine phosphokinase. The level of antibody against the acetylcholine receptor increased 4.1-fold. His condition progressed rapidly resulting in respiratory failure 15 days after conservative therapy., 論文
- Published
- 2020
38. [Primary Intracranial Malignant Lymphoma Associated with Acquired Immunodeficiency Syndrome(AIDS):A Case Report]
- Author
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Yasufumi, Inaka, Naoki, Otani, Sho, Nishida, Kazuya, Fujii, Hideaki, Ueno, Satoshi, Tomura, Arata, Tomiyama, Hideo, Osada, Kojiro, Wada, Takuya, Maeda, and Kentaro, Mori
- Subjects
Male ,Acquired Immunodeficiency Syndrome ,Treatment Outcome ,Brain Neoplasms ,Biopsy ,Humans ,Lymphoma, Large B-Cell, Diffuse ,Middle Aged ,Craniotomy - Abstract
The spread of human immunodeficiency virus(HIV)infection may result in an increased likelihood of surgery in patients with HIV infection. We treated a patient with intracranial malignant lymphoma associated with acquired immunodeficiency syndrome(AIDS)caused by HIV infection. The recommendations of the countermeasure manual for AIDS were followed. Only surgical staff without finger injury or inflammation were permitted to be involved in the operation. All staff were dressed in a waterproof, full-body surgical gown, and wore double gloves, double foot covers, and an N95 mask. The surgery could be performed safely with such infection control measures. Histological examination revealed a diffuse large B-cell lymphoma. The patient was referred to the Division of Infectious Diseases and Respiratory Medicine for chemotherapy.
- Published
- 2017
39. A decrease in phytic acid content substantially affects the distribution of mineral elements within rice seeds
- Author
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Chie Matsubara, Kaoru T. Yoshida, Hiroaki Sakai, Osamu Yatou, Sho Nishida, Yuto Usui, Toru Iwai, Yasuko Terada, Naohiro Aoki, and Masaki Okamura
- Subjects
Phytic Acid ,Potassium ,chemistry.chemical_element ,Plant Science ,Oryza ,Endosperm ,chemistry.chemical_compound ,Quantitative Trait, Heritable ,Aleurone ,Botany ,Genetics ,Minerals ,Phytic acid ,Oryza sativa ,biology ,Phosphorus ,food and beverages ,General Medicine ,Elements ,Plants, Genetically Modified ,biology.organism_classification ,Genetically modified rice ,Phenotype ,chemistry ,Mutation ,Seeds ,lipids (amino acids, peptides, and proteins) ,Agronomy and Crop Science - Abstract
Phytic acid (myo-inositol hexakisphosphate; InsP6) is the storage compound of phosphorus and many mineral elements in seeds. To determine the role of InsP6 in the accumulation and distribution of mineral elements in seeds, we performed fine mappings of mineral elements through synchrotron-based X-ray microfluorescence analysis using developing seeds from two independent low phytic acid (lpa) mutants of rice (Oryza sativa L.). The reduced InsP6 in lpa seeds did not affect the translocation of mineral elements from vegetative organs into seeds, because the total amounts of phosphorus and the other mineral elements in lpa seeds were identical to those in the wild type (WT). However, the reduced InsP6 caused large changes in mineral localization within lpa seeds. Phosphorus and potassium in the aleurone layer of lpa greatly decreased and diffused into the endosperm. Zinc and copper, which were broadly distributed from the aleurone layer to the inner endosperm in the WT, were localized in the narrower space around the aleurone layer in lpa mutants. We also confirmed that similar distribution changes occurred in transgenic rice with the lpa phenotype. Using these results, we discussed the role of InsP6 in the dynamic accumulation and distribution patterns of mineral elements during seed development.
- Published
- 2015
40. Induction of Nickel Accumulation in Response to Zinc Deficiency in Arabidopsis thaliana
- Author
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Takafumi Mizuno, Sho Nishida, Chisato Tsuzuki, Junko Yoshida, and Aki Kato
- Subjects
inorganic chemicals ,Arabidopsis thaliana ,Iron ,Mutant ,Arabidopsis ,chemistry.chemical_element ,Zinc ,Plant Roots ,Article ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,nickel ,Hydroponics ,Gene Expression Regulation, Plant ,Botany ,medicine ,Physical and Theoretical Chemistry ,Cation Transport Proteins ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,biology ,Arabidopsis Proteins ,Gene Expression Profiling ,fungi ,zinc ,Organic Chemistry ,Wild type ,food and beverages ,Biological Transport ,Transporter ,General Medicine ,medicine.disease ,biology.organism_classification ,Molecular biology ,Computer Science Applications ,Transport protein ,Agar ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,transporter ,Mutation ,Zinc deficiency - Abstract
Excessive accumulation of nickel (Ni) can be toxic to plants. In Arabidopsis thaliana, the Fe2+ transporter, iron (Fe)-regulated transporter1 (IRT1), mediates Fe uptake and also implicates in Ni2+ uptake at roots, however, the underlying mechanism of Ni2+ uptake and accumulation remains unelucidated. In the present study, we found that zinc (Zn) deficient conditions resulted in increased accumulation of Ni in plants, particularly in roots, in A. thaliana. In order to elucidate the underlying mechanisms of Ni uptake correlating zinc condition, we traced 63Ni isotope in response to Zn and found that (i) Zn deficiency induces short-term Ni2+ absorption and (ii) Zn2+ inhibits Ni2+ uptake, suggesting competitive uptake between Ni and Zn. Furthermore, the Zrt/Irt-like protein 3 (ZIP3)-defective mutant with an elevated Zn-deficient response exhibited higher Ni accumulation than the wild type, further supporting that the response to Zn deficiency induces Ni accumulation. Previously, expression profile study demonstrated that IRT1 expression is not inducible by Zn deficiency. In the present study, we found increased Ni accumulation in IRT1-null mutant under Zn deficiency in agar culture. These suggest that Zn deficiency induces Ni accumulation in an IRT1-independen manner. The present study revealed that Ni accumulation is inducible in response to Zn deficiency, which may be attributable to a Zn uptake transporter induced by Zn deficiency.
- Published
- 2015
41. Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds
- Author
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Sho Nishida, Tomoe Shimizu, Yusuke Tagashira, Masanobu Miyamoto, and Kaoru T. Yoshida
- Subjects
Transgene ,Mutant ,translocation ,molecular breeding ,Plant Science ,Article ,ectopic expression ,chemistry.chemical_compound ,Biosynthesis ,mineral element ,Oryza sativa L ,phosphorus ,Ecology, Evolution, Behavior and Systematics ,Phytic acid ,Oryza sativa ,Ecology ,biology ,Botany ,food and beverages ,biology.organism_classification ,Genetically modified rice ,phytic acid ,chemistry ,Biochemistry ,Seedling ,QK1-989 ,Phytase ,seed - Abstract
The manipulation of seed phosphorus is important for seedling growth and environmental P sustainability in agriculture. The mechanism of regulating P content in seed, however, is poorly understood. To study regulation of total P, we focused on phytic acid (inositol hexakisphosphate, InsP6) biosynthesis-related genes, as InsP6 is a major storage form of P in seeds. The rice (Oryza sativa L.) low phytic acid mutant lpa1-1 has been identified as a homolog of archael 2-phosphoglycerate kinase. The homolog might act as an inositol monophosphate kinase, which catalyzes a key step in InsP6 biosynthesis. Overexpression of the homolog in transgenic rice resulted in a significant increase in total P content in seed, due to increases in InsP6 and inorganic phosphates. On the other hand, overexpression of genes that catalyze the first and last steps of InsP6 biosynthesis could not increase total P levels. From the experiments using developing seeds, it is suggested that the activation of InsP6 biosynthesis in both very early and very late periods of seed development increases the influx of P from vegetative organs into seeds. This is the first report from a study attempting to elevate the P levels of seed through a transgenic approach.
- Published
- 2015
42. Two Distinct Families of Protein Kinases Are Required for Plant Growth under High External Mg2+ Concentrations in Arabidopsis
- Author
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Toru Fujiwara, Junya Mizoi, Sho Nishida, Yoshifumi Tsukiori, Satoshi Kidokoro, Takuya Yoshida, Fuminori Takahashi, Kazuo Shinozaki, Yasunari Fujita, Kazuko Yamaguchi-Shinozaki, Hirofumi Nakagami, Yuko Nomura, Shuichi Yanagisawa, Junro Mogami, Tetsuya Ishida, and Kyoko Morimoto
- Subjects
biology ,Physiology ,Immunoprecipitation ,fungi ,food and beverages ,Plant Science ,biology.organism_classification ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Arabidopsis ,Cellular ion homeostasis ,Genetics ,Arabidopsis thaliana ,Phosphorylation ,Protein phosphorylation ,Signal transduction ,Abscisic acid - Abstract
Protein phosphorylation events play key roles in maintaining cellular ion homeostasis in higher plants, and the regulatory roles of these events in Na+ and K+ transport have been studied extensively. However, the regulatory mechanisms governing Mg2+ transport and homeostasis in higher plants remain poorly understood, despite the vital roles of Mg2+ in cellular function. A member of subclass III sucrose nonfermenting-1-related protein kinase2 (SnRK2), SRK2D/SnRK2.2, functions as a key positive regulator of abscisic acid (ABA)-mediated signaling in response to water deficit stresses in Arabidopsis (Arabidopsis thaliana). Here, we used immunoprecipitation coupled with liquid chromatography-tandem mass spectrometry analyses to identify Calcineurin B-like-interacting protein kinase26 (CIPK26) as a novel protein that physically interacts with SRK2D. In addition to CIPK26, three additional CIPKs (CIPK3, CIPK9, and CIPK23) can physically interact with SRK2D in planta. The srk2d/e/i triple mutant lacking all three members of subclass III SnRK2 and the cipk26/3/9/23 quadruple mutant lacking CIPK26, CIPK3, CIPK9, and CIPK23 showed reduced shoot growth under high external Mg2+ concentrations. Similarly, several ABA biosynthesis-deficient mutants, including aba2-1, were susceptible to high external Mg2+ concentrations. Taken together, our findings provided genetic evidence that SRK2D/E/I and CIPK26/3/9/23 are required for plant growth under high external Mg2+ concentrations in Arabidopsis. Furthermore, we showed that ABA, a key molecule in water deficit stress signaling, also serves as a signaling molecule in plant growth under high external Mg2+ concentrations. These results suggested that SRK2D/E/I- and CIPK26/3/9/23-mediated phosphorylation signaling pathways maintain cellular Mg2+ homeostasis.
- Published
- 2015
43. Usefulness of Suction Decompression through Direct Puncture of the Common Carotid Artery during Clipping of a Paraclinoid Internal Carotid Artery Aneurysm
- Author
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Sho Nishida, Satoshi Tomura, Kentaro Mori, Naoki Otani, Terushige Toyooka, Takuji Yamamoto, Hideo Osada, Kosuke Kumagai, Yasufumi Inaka, Kojiro Wada, Yasuaki Nakao, and Kazuya Fujii
- Subjects
medicine.medical_specialty ,Decompression ,business.industry ,Direct puncture ,medicine.artery ,medicine.medical_treatment ,medicine ,Internal carotid artery aneurysm ,Common carotid artery ,Radiology ,Clipping (medicine) ,business ,Surgery - Published
- 2015
44. [A Case of Foramen Magnum Meningioma Manifesting as Hypoglossal Nerve Palsy]
- Author
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Yasufumi, Inaka, Naoki, Otani, Sho, Nishida, Hideaki, Ueno, Arata, Tomiyama, Satoshi, Tomura, Terushige, Toyooka, Kojiro, Wada, and Kentaro, Mori
- Subjects
Treatment Outcome ,Meningeal Neoplasms ,Humans ,Female ,Foramen Magnum ,Hypoglossal Nerve Diseases ,Meningioma ,Magnetic Resonance Imaging ,Skull Base Neoplasms ,Aged - Abstract
We report a case of foramen magnum meningioma manifesting as hypoglossal nerve palsy. A 72-year-old woman presented with progressive hypoglossal nerve palsy and lingual atrophy on the left side. Gadolinium-enhanced T1-weighted magnetic resonance imaging revealed a heterogeneously enhanced mass lesion with dural tail sign partially extending into the hypoglossal canal. The transcondylar approach was performed to expose the hypoglossal canal and resect the tumor completely. Histological examination revealed a transitional meningioma. The postoperative course was uneventful. Hypoglossal nerve palsy improved gradually after the operation.
- Published
- 2017
45. Selective induction of putative iron transporters,OPT8a and OPT8b, in maize by mycorrhizal colonization
- Author
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Toru Fujiwara, Natsuko I. Kobayashi, Yoshihiro Ohmori, Rie Tomioka, Sho Nishida, Yoshihiro Kobae, and Keitaro Tanoi
- Subjects
Iron uptake ,Nutrient ,fungi ,Botany ,Gene expression ,Soil Science ,Colonization ,Transporter ,Plant Science ,Biology ,Arbuscular mycorrhizal ,Gene ,Mycorrhizal colonization - Abstract
Arbuscular mycorrhizas support nutrient uptake from the soil. Here we demonstrated Iron-59 (59Fe) uptake in maize (Zea mays L.) through the mycorrhizal roots. Arbuscular mycorrhizal colonization did not strongly induce Strategy II-related genes, but the putative iron transporter genes, OPT8a and OPT8b, were induced by more than 50-fold. Our data provide a previously undescribed gene expression mode related to the iron uptake system of Strategy II plants.
- Published
- 2014
46. Potential Risks and Limited Indications of the Supraorbital Keyhole Approach for Clipping Internal Carotid Artery Aneurysms
- Author
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Arata Tomiyama, Terushige Toyooka, Takuji Yamamoto, Satoshi Tomura, Naoki Otani, Sho Nishida, Yasuaki Nakao, Kojiro Wada, Satoru Takeuchi, Hideaki Ueno, and Kentaro Mori
- Subjects
MRI, Magnetic resonance imaging ,AntChoA, Anterior choroidal artery ,medicine.medical_treatment ,lcsh:RC346-429 ,UCA, Unruptured cerebral aneurysm ,HAM-D, Hamilton Depression Scale ,Modified Rankin Scale ,Retrospective analysis ,DSA, Digital subtraction angiography ,MMSE, Mini-Mental Status Examination ,NIHSS, National Institutes of Health Stroke Scale ,Clipping ,surgical procedures, operative ,Status examination ,cardiovascular system ,CT, Computed tomography ,Original Article ,BDI, Beck Depression Inventory ,ICA, Internal carotid artery ,PcomA, Posterior communicating artery ,Internal carotid artery ,mRS, Modified Rankin Scale ,medicine.medical_specialty ,Unruptured cerebral aneurysm ,education ,lcsh:Surgery ,Keyhole surgery ,Aneurysm ,AcomA, Anterior communicating artery ,medicine.artery ,medicine ,cardiovascular diseases ,DWI, Diffusion-weighted imaging ,lcsh:Neurology. Diseases of the nervous system ,3D, 3-Dimensional ,ISUIA, International Study of Unruptured Intracranial Aneurysms ,MCA, Middle cerebral artery ,business.industry ,BDI - Beck depression inventory ,lcsh:RD1-811 ,Clipping (medicine) ,medicine.disease ,nervous system diseases ,Surgery ,CTA, Computed tomography angiography ,Neurology (clinical) ,business ,HDS-R, Revised Hasegawa Dementia Scale ,Keyhole - Abstract
Background: Internal carotid artery (ICA) aneurysm may be a good target for supraorbital keyhole clipping. We discuss the surgical indications and risks of keyhole clipping for ICA aneurysms based on long-term clinical and radiologic results. Methods: This was a retrospective analysis of 51 patients (aged 35–75 years, mean 62 years) with ICA aneurysms (mean 5.8 ± 1.8 mm) who underwent clipping via the supraorbital keyhole approach between 2005 and 2017. Neurologic and cognitive functions were examined by several methods, including the modified Rankin Scale and Mini-Mental Status Examination. The state of clipping was assessed 1 year and then every few years after the operation. Results: Complete clipping was confirmed in 45 patients (88.2%), dog-ear remnants behind the clip persisted in 4 patients, and wrapping was performed in 2 patients. Mean duration of postoperative hospitalization was 3.4 ± 6.9 days. The mean clinical follow-up period was 6.6 ± 3.2 years. The overall mortality was 0, and overall morbidity (modified Rankin Scale score ≥2 or Mini-Mental Status Examination
- Published
- 2019
47. The effect of fertilization on cesium concentration of rice grown in a paddy field in Fukushima Prefecture in 2011 and 2012
- Author
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Toru Fujiwara, Yoshihiro Ohmori, Masataka Kajikawa, Keitaro Tanoi, Nobuhiro Tanaka, Sho Nishida, Jun Furukawa, and Natsuko I. Kobayashi
- Subjects
Nitrogen ,Potassium ,chemistry.chemical_element ,Plant Science ,engineering.material ,Biology ,Soil ,Human fertilization ,Japan ,Species Specificity ,Radiation Monitoring ,Cesium Isotopes ,Fukushima Nuclear Accident ,Soil Pollutants, Radioactive ,Cultivar ,Fertilizers ,Plant Stems ,food and beverages ,Agriculture ,Oryza ,Contamination ,Straw ,Biodegradation, Environmental ,Agronomy ,chemistry ,Cesium Radioisotopes ,Nuclear Power Plants ,Seeds ,engineering ,Paddy field ,Brown rice ,Fertilizer - Abstract
After the accident of the Fukushima 1 nuclear power plant in March 2011, radioactive cesium was released and paddy field in a wide area of Fukushima Prefecture was contaminated. To reduce radioactive Cs uptake by rice, it is important to understand factors that affect Cs uptake in rice. Here we describe our study in 2011 and 2012 to investigate Cs concentration in two rice cultivars, Koshihikari and Hitomebore, the top two cultivars in Fukushima prefecture, grown under different fertilizer conditions in the contaminated paddy field. Our study demonstrated that high nitrogen and low potassium conditions increase Cs concentrations both in straw and brown rice.
- Published
- 2013
48. Epigenetic Regulation of Organic Anion Transporting Polypeptide 1B3 in Cancer Cell Lines
- Author
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Sotaro Naoi, Hiroyuki Kusuhara, Yuri Tsuruya, Sho Nishida, Yuichi Sugiyama, Ryota Kikuchi, and Satoki Imai
- Subjects
Organic anion transporter 1 ,Pharmaceutical Science ,Organic Anion Transporters, Sodium-Independent ,Biology ,Cell Line ,Epigenesis, Genetic ,Solute Carrier Organic Anion Transporter Family Member 1B3 ,Cell Line, Tumor ,Neoplasms ,Humans ,Gene silencing ,Pharmacology (medical) ,RNA, Messenger ,Epigenetics ,Pharmacology ,Regulation of gene expression ,Organic Chemistry ,DNA Methylation ,Molecular biology ,Cell biology ,DNA-Binding Proteins ,Gene Expression Regulation, Neoplastic ,Organic anion-transporting polypeptide ,Cell culture ,Cancer cell ,DNA methylation ,biology.protein ,Molecular Medicine ,RNA Interference ,Biotechnology - Abstract
The expression of a multispecific organic anion transporter, OATP1B3/SLCO1B3, is associated with clinical prognosis and survival of cancer cells. The aims of present study were to investigate the involvement of epigenetic regulation in mRNA expression of a cancer-type variant of OATP1B3 (Ct-OATP1B3) in cancer cell lines. The membrane localization and transport functions of Ct-OATP1B3 were investigated in HEK293 cells transiently expressing Ct-OATP1B3. DNA methylation profiles around the transcriptional start site of Ct-OATP1B3 in cancer cell lines were determined. The effects of a DNA methyltransferase inhibitor and siRNA knockdown of methyl-DNA binding proteins (MBDs) on the expression of Ct-OATP1B3 mRNA were investigated. 5′-RACE identified the TSS of Ct-OATP1B3 in PK-8 cells. Ct-OATP1B3 was localized on the plasma membrane, and showed the transport activities of E217βG, fluvastatin, rifampicin, and Gd-EOB-DTPA. The CpG dinucleotides were hypomethylated in Ct-OATP1B3-positive cell lines (DLD-1, TFK-1, PK-8, and PK-45P) but were hypermethylated in Ct-OATP1B3-negative cell lines (HepG2 and Caco-2). Treatment with a DNA methyltransferase inhibitor and siRNA knockdown of MBD2 significantly increased the expression of Ct-OATP1B3 mRNA in HepG2 and Caco-2. Ct-OATP1B3 is capable of transporting its substrates into cancer cells. Its mRNA expression is regulated by DNA methylation-dependent gene silencing involving MBD2.
- Published
- 2013
49. Genome-wide analysis of specific alterations in transcript structure and accumulation caused by nutrient deficiencies in Arabidopsis thaliana
- Author
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Yusuke Kakei, Sho Nishida, Toru Fujiwara, and Yukihisa Shimada
- Subjects
0106 biological sciences ,0301 basic medicine ,Gene isoform ,Nitrogen ,Amino Acid Motifs ,Arabidopsis ,Plant Science ,Biology ,01 natural sciences ,Plant Roots ,Transcriptome ,03 medical and health sciences ,Exon ,Genetics ,MYB ,Gene ,Arabidopsis Proteins ,Sequence Analysis, RNA ,Alternative splicing ,RNA ,Computational Biology ,Phosphorus ,Cell Biology ,Exons ,Alternative Splicing ,030104 developmental biology ,Gene Ontology ,Metals ,Transcription Factor Gene ,Sulfur ,010606 plant biology & botany - Abstract
Summary The alteration of transcript structure contributes to transcriptome plasticity. In this study, we analyzed the genome-wide response of exon combination patterns to deficiencies in 12 different nutrients in Arabidopsis thaliana roots. RNA sequencing analysis and bioinformatics using a simulation survey revealed more than 600 genes showing varying exon combinations. The overlap between genes showing differential expression (DE) and genes showing differential exon combination (DC) was notably low. Additionally, gene ontology analysis showed that gene functions were not shared between the DE and DC genes, suggesting that the genes showing DC had different roles than those showing DE. Most of the DC genes were nutrient specific. For example, two homologs of the MYB transcription factor genes MYB48 and MYB59 showed differential alternative splicing only in response to low levels of potassium. Alternative splicing of those MYB genes modulated DNA-binding motifs, and MYB59 is reportedly involved in the inhibition of root elongation. Therefore, the increased abundance of MYB isoforms with an intact DNA-binding motif under low potassium may be involved in the active inhibition of root elongation. Overall, we provide global and comprehensive data for DC genes affected by nutritional deficiencies, which contribute to elucidating an unknown mechanism involved in adaptation to nutrient deficiency.
- Published
- 2016
50. Synthesis and Biological Activity of Some New Pyrazole Copper(II) Complexes
- Author
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Fumi Okabe, Ryoko Tomita, Fumio Miake, Sho Nishida, Toshihiro Fujioka, Nobuhiro Kashige, Masahiko Omori, Kenji Yamagata, Hiroshi Maruoka, Eiichi Masumoto, and Yuki Yoshimura
- Subjects
biology ,Chemistry ,Stereochemistry ,Organic Chemistry ,chemistry.chemical_element ,Biological activity ,Pyrazole ,biology.organism_classification ,Copper ,In vitro ,Sodium hydride ,HeLa ,chemistry.chemical_compound ,Transition metal ,IC50 - Abstract
As a part of systematic investigation of synthesis and biologically active compounds of pyrazole derivatives containing transition metal, several new pyrazole copper(II) complexes 3a−f were synthesized from pyrazole sodium salts 2a−f, which were produced from spiro-pyrazoles 1a−f and sodium hydride by a ring-opening reaction. All the synthesized compounds were characterized by spectroscopic analysis. Pyrazole copper(II) complexes 3a−d and 3f exhibited high DNA cleavage activity in vitro. Furthermore, compounds 3a−f were tested for their growth inhibitory activity in A549 lung cancer, B16F10 murine melanoma, and HeLa human uterine carcinoma cells. Compounds 3c,d displayed moderate B16F10 and HeLa inhibitory activity levels (3c: IC50 = 45 μM in B16F10 cells and 34 μM in HeLa cells, 3d: IC50 = 50 μM in B16F10 cells and 32 μM in HeLa cells).
- Published
- 2012
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