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This paper proposes a control method for balancing the voltages of series-connected SiC MOSFETs based on active clamping and comparison circuitry. Each SiC MOSFET is paired with an active clamping module (ACM), which comprises an auxiliary switch and a capacitor. Equal voltage distribution across the SiC MOSFETs is achieved by maintaining balance among the voltages of the ACM capacitors. This voltage balance is achieved through a local comparison circuit with the threshold set at the blocking voltage of the SiC MOSFET in its OFF state. When the ACM capacitor voltage exceeds this threshold, the auxiliary switch is triggered on during the dead time, and the excess energy of this capacitor is recycled back to the DC bus. The proposed adaptive balancing process maintains all ACM capacitor voltages at the threshold without requiring system-level coordination or communication between ACMs, thus simplifying the control circuits. The method does not affect the SiC MOSFETs’ switching speed and enables zero-voltage switching on for the auxiliary switches. Comprehensive operating principles and key parameter designs are provided for both DC transformer (DCT) and Voltage Source Converter (VSC) applications. A high-voltage power module, incorporating six SiC MOSFETs in series, was constructed to demonstrate the effectiveness of the proposed approach. Tests on a DCT and a VSC incorporating this high-power module validated the efficacy of the proposed voltage balancing method.

Millimeter-wave (mmWave) synthetic aperture radar (SAR) has found wide applications in autonomous driving, landslide detection, urban mapping, etc. However, the high propagation loss of mmWave bands and the limitation of transmitting power have led to limited imaging performance for mmWave SAR. In this article, an enhanced SAR imaging framework that combines along-track multiple channels is proposed using a low-rank tensor-train (TT) decomposition method, which is applicable for a co-located multiple input multiple output (MIMO) array or a phased-transmitting-digital-receiving array. First, the multichannel images are stacked into a tensor form after SAR imaging on individual channels and spatial variant array phase correction for each pixel. Then, the low-rank property of tensor stack is exploited and the TT model is utilized to find the redundancy and leverage the intrinsic structure of image stack. In addition, ket augmentation is introduced to exhibit the local data structure more clearly than the original tensor under TT decomposition. Finally, tensor-train nuclear norm is used to relax the NP-hard problem with low-rank constraint and the minimization problem is solved in the framework of alternating direction method of multipliers for enhanced imaging. The proposed algorithm can effectively improve the working distance and image quality of mmWave SAR. Numerical experiments using simulated data of MIMO SAR and measured data collected by a ground-based phased-transmitting-digital-receiving array system are carried out to verify the performance of the proposed algorithm.

Abstract Background Idiopathic Pulmonary Fibrosis (IPF) is a fatal disease with scarce therapeutic alternatives, which imposes a significant economic burden on society. The identification of novel drug targets is thus critically essential. Plasma proteins with discernible causal evidence hold promise as viable drug targets for this condition. Methods We performed a proteome-wide Mendelian randomization (MR) analysis to assess the causal effects of 4,907 circulating proteins from the deCODE study on the risk of IPF from the Finngen Database (2,018 cases vs. 373,064 controls). We further replicated the MR analysis in 1426 proteins from the ARIC study and IPF from the UK Biobank (1,369 cases vs. 435,866 controls). Then a series of analyses including Bayesian colocalization, Steiger filtering, and phenotype scanning were conducted to validate the credibility of the MR results. Subsequently, protein-protein interaction (PPI) analysis, pathway enrichment analysis, and druggability assessment were executed to elucidate the underlying mechanisms. Finally, the findings were corroborated using a bleomycin-induced pulmonary fibrosis mouse model. Results The MR analysis bolstered by robust evidence of colocalization, indicated a significant positive association between Prothrombin and increased IPF risk (OR = 3.26,95%CI 1.75–6.07). Conversely, Bone Sialoprotein (IBSP) demonstrated an inverse association with IPF susceptibility (OR = 0.27,95%CI 0.14–0.55). Conclusions The integrative analysis suggests that Prothrombin and IBSP are promising candidates as potential drug targets for IPF. Additional clinical investigations are warranted to substantiate these findings.

Abstract The study explored teacher–student interactions in foreign language teaching classes in China, aiming to identify ways to improve these interactions. Data were collected through 180 min of unstructured observations in two teaching classes. The study found that the frequency of interactions varied among teachers. After analysing the data with a language socialisation theory, the study revealed the factors that affected teacher–student interactions in foreign language classes in China: firstly, the concept of an equal teacher–student relationship is the precondition of teacher–student interactions; secondly, paying attention to both language acquisition and culture acquisition is the foundation of teacher–student interactions; thirdly, a good English acquisition context is one of the critical elements of conducting teacher−student interactions. The study delivered suggestions to promote teacher–student interactions based on English education in China. As there are many similarities in the English education policy and pedagogy between countries in Asia, the findings of the current study can be referenced by other Asian countries as well.

In this study, the tensile behaviors of laser powder bed fused (L-PBF) and laser powder directed energy deposited (LP-DED) HAYNES® 214 were investigated at a wide range of test temperatures (−195, 25, 425, 650, 870 and 980 °C). Specimens fabricated in the vertical orientation were subjected to a three-step heat treatment including stress relief, hot isostatic pressing, and solution treatment. The non-heat treated microstructures of L-PBF and LP-DED specimens were dendritic and columnar, oriented in the build direction. Recrystallization occurred after solution annealing, with the nucleation and growth of equiaxed grains. Tensile results revealed higher strength for the L-PBF specimens compared to LP-DED up to 650 °C, attributed to their finer grain size. The highest strengths were recorded at cryogenic temperatures, where diminished dislocation mobility and deformation twinning provided increased resistance to plastic deformation. From room temperature to 425 °C, a gradual decrease in strength was ascribed to the increase in dislocation motion with temperature. Ductility was measured to be similar for both processes up to 650 °C, and fracture surface analysis showed transgranular failure at these temperatures. A ductility dip was observed between temperatures of 650–870 °C due to the activation of grain boundary sliding resulting in intergranular cracking. The heterogeneous grain size distribution in both L-PBF and LP-DED specimens led to the ductility being comparable for both processes. The highest test temperature of 980 °C introduced sufficient thermal energy to promote significant grain boundary sliding which resulted in intergranular fracture in the L-PBF and LP-DED specimens.

Abstract Background Thermal ablation is a minimally invasive treatment for non-small cell lung cancer (NSCLC). Aside from causing an immediate direct tumour cell injury, the effects of thermal ablation on the internal microenvironment are unknown. This study aimed to investigate the effects of thermal ablation on the plasma internal environment in patients with NSCLC. Methods 128 plasma samples were collected from 48 NSCLC (pre [LC] and after thermal ablation [LC-T]) patients and 32 healthy controls (HCs). Olink proteomics and metabolomics were utilized to construct an integrated landscape of the cancer-related immune and inflammatory responses after ablation. Results Compared with HCs, LC patients exhibited 58 differentially expressed proteins (DEPs) and 479 differentially expressed metabolites (DEMs), which might participate in tumour progression and metastasis. Moreover, 75 DEPs were identified among the HC, LC, and LC-T groups. Forty-eight highly expressed DEPs (eg, programmed death-ligand 1 [PD-L1]) in the LC group were found to be downregulated after thermal ablation. These DEPs had significant impacts on pathways such as angiogenesis, immune checkpoint blockade, and pro-tumour chemotaxis. Metabolites involved in tumour cell survival were associated with these proteins at the expression and functional levels. In contrast, 19 elevated proteins (eg, interleukin [IL]-6) were identified after thermal ablation. These proteins were mainly associated with inflammatory response pathways (NF-κB signalling and tumour necrosis factor signalling) and immune cell activation. Conclusions Thermal ablation-induced changes in the host plasma microenvironment contribute to anti-tumour immunity in NSCLC, offering new insights into tumour ablation combined with immunotherapy. Trial registration This study was registered on the Chinese Clinical Trial Registry ( https://www.chictr.org.cn/index.html ). ID: ChiCTR2300076517. Registration Date: 2023-10-11.

Abstract The skin epidermis is continually influenced by a myriad of internal and external elements. At its basal layer reside epidermal stem cells, which fuels epidermal renovation and hair regeneration with powerful self-renewal ability, as well as keeping diverse signals that direct their activity under surveillance with quick response. The importance of epidermal stem cells in wound healing and immune-related skin conditions has been increasingly recognized, and their potential for clinical applications is attracting attention. In this review, we delve into recent advancements and the various physiological and psychological factors that govern distinct epidermal stem cell populations, including psychological stress, mechanical forces, chronic aging, and circadian rhythm, as well as providing an overview of current methodological approaches. Furthermore, we discuss the pathogenic role of epidermal stem cells in immune-related skin disorders and their potential clinical applications.

With the recent implementation of additively manufactured parts into industrial applications, there is a dire need for nondestructive evaluation methods to qualify if these components are fit for service due to their sensitivity to processing conditions. The Impulse Excitation Technique (IET) is applied to additively manufactured Ti-6Al-4V bending specimens to determine natural frequencies and damping properties in order to predict fatigue performance relative to specimens fabricated with different processing parameters. From the damping and natural frequency results, it was found that the specimens, fabricated with intentional underheating to induce lack of fusion defects, had the lowest damping value in the pristine condition and the highest natural frequency. For the three batches of specimens tested, it was determined that the underheated specimens had the best fully-reversed bending fatigue performance with the highest fatigue limit (297 MPa) and longest fatigue lives as compared to the other two batches, implying a relation of decreased fatigue life with increased material damping in the pristine condition. The theory of the IET related to materials is presented with damping and fatigue results, as well as microstructural analysis and fractography of three specimens batches fabricated with different processing parameters.

Deteriorated filter condition in laser powder bed fusion (L-PBF) systems may negatively impact shield gas flow, causing inadequate spatter particle/plume removal, leading to laser beam attenuation and reduction in melt pool depth, and potentially causing more frequent formation of volumetric defects. This work investigated the effects of filter condition and part location on the micro-/defect-structure and mechanical behavior, including tensile and fatigue, of Ti-6Al-4V parts fabricated by L-PBF. Interestingly, within the manufacturer recommended service intervals, no specific effect of filter condition could be observed on the micro-/defect-structure or the mechanical behavior of the fabricated parts. However, the parts’ defect-structures were affected by their location, with ones located near the center of the build plate having less porosity than the ones located away. Although these defects did not affect the tensile properties, they frequently observed to initiate fatigue cracks (the critical defects sizes were often in the range of a few tens of micrometers). Therefore, their sensitivity to location resulted in the location dependence of the fatigue behavior.

IntroductionFusarium-induced root rot of Carya cathayensis (C. cathayensis) is a typical soil-borne disease that has severely damaged the Carya cathayensis industry in China. Understanding the interaction among soil microbial communities, soil characteristics, and pathogenic bacteria is very important for the ecological prevention and control of Carya cathayensis root rot.MethodsWe used Miseq Illumina high-throughput sequencing technology to study the microbial community in the rhizosphere soil of healthy and diseased C. cathayensis, quantified the abundance of bacteria, fungi, and pathogenic fungi, and combined these with soil chemistry and enzyme activity indicators to analyze the characteristics of healthy and diseased rhizosphere soils.ResultsWe found that the pH, soil organic carbon(SOC), available nitrogen (AN), available phosphorus (AP), available potassium (AK),N-acetyl-β-D-glucosaminidase (NAG) β-glucosidase (BG), fungal gene copy number, bacterial community diversity and network complexity of the diseased soil were significantly lower (p

Abstract During our previous bilateral adrenal vein sampling (AVS) procedure, the authors observed that accessing the left adrenal vein through the antecubital vein was more feasible than the conventional femoral vein. Meanwhile, the femoral vein pathway facilitated access to the right adrenal vein than the antecubital vein pathway. Therefore, the authors hypothesized that simultaneous bilateral AVS via the antecubital combined with the femoral vein pathway could improve the success rate. A total of 94 cases of AVS via the antecubital combined with the femoral vein pathway were performed, while the remaining 20 cases employed the antecubital vein pathway at our center between August 2020 and April 2023. Furthermore, a meta‐analysis was conducted in this study using 15 selected articles to determine the success rate of AVS in each center and pathway. The success rate of ACTH‐stimulated simultaneous bilateral AVS via the antecubital vein combined with the femoral vein pathway was 92.85% (P = .503) on the right and 95.00% (P

Abstract Cancer cells recruit neutrophils from the bloodstream into the tumor tissue, where these immune cells promote the progression of numerous solid tumors. Studies in mice suggest that blocking neutrophil recruitment to tumors by inhibition of neutrophil chemokine receptor CXCR2 could be a potential immunotherapy for pancreatic cancer. Yet, the mechanisms by which neutrophils promote tumor progression in humans, as well as how CXCR2 inhibition could potentially serve as a cancer therapy, remain elusive. In this study, we developed a human cell-based microphysiological system to quantify neutrophil-tumor spheroid interactions in both “separated” and “contact” scenarios. We found that neutrophils promote the invasion of tumor spheroids through the secretion of soluble factors and direct contact with cancer cells. However, they promote the proliferation of tumor spheroids solely through direct contact. Interestingly, treatment with AZD-5069, a CXCR2 inhibitor, attenuates invasion and proliferation of tumor spheroids by blocking direct contact with neutrophils. Our findings also show that CXCR2 inhibition reduces neutrophil migration toward tumor spheroids. These results shed new light on the tumor-promoting mechanisms of human neutrophils and the tumor-suppressive mechanisms of CXCR2 inhibition in pancreatic cancer and may aid in the design and optimization of novel immunotherapeutic strategies based on neutrophils.

Leaf anatomical traits are susceptible to environmental changes and can reflect plant adaptation strategies to the environment. Populus euphratica plays a key role in maintaining ecosystem processes and functions in arid zones, but the variations in leaf anatomical traits of Populus euphratica and their drivers at large scales remain unclear. Here, we investigated 10 leaf anatomical traits of Populus euphratica from 12 sampling sites in the arid zone of China, and explored how they were affected by geographic, climatic, and soil factors. Our results showed that these traits differed significantly between sites (P < 0.05), which was mainly determined by climate and soil factors that together explained 41.4 % of the trait variation. The impact of climate factors is the most critical, as they can directly influence the variation in leaf anatomical traits and indirectly affect trait variation by influencing soil factors. Among them, the max temperature of the warmest month (BIO5), soil available phosphorus (SAP) and soil moisture content (SM) had the most critical effect. Besides, temperature was the major influencing factor for the ratio of spongy tissue thickness to leaf thickness (SR), palisade tissue thickness (PT), the ratio of palisade tissue thickness to spongy tissue thickness (PSR), and the ratio of palisade tissue thickness to leaf thickness (CTR), while soil nutrient was the major influencing factor for leaf thickness (LT), lower epidermis thickness (LET), and spongy tissue thickness (ST). Our results provide important insights into the response of dryland forests to climate change, and suggest that climate warming and declining soil quality may have a stronger effect on Populus euphratica.

During freeze–thaw cycling, aggregates undergo a dynamic change in breakdown–formation (turnover), however, how the turnover occurs between aggregates of various particle sizes is not clear. To clarify the influence of freeze–thaw cycling on the dynamic changes in the particle size of soil aggregates, soil aggregates from the Black Soil Region of Northeast China were selected as the research objects. The study conducted in situ dynamic monitoring experiments, innovatively applying the rare earth oxide (REO) tracer method to natural conditions of freeze–thaw cycles (autumn freeze–thaw period, freezing period, and spring freeze–thaw period), accurately tracking the turnover paths and quantifying the turnover rates between aggregates of various particle sizes. The results revealed that the total value of the formation paths of the 2–5 mm aggregates and 0.25–2 mm aggregates increased during the autumn freeze–thaw period. The number of freeze–thaw cycles and accumulated snowfall were significantly positively correlated with aggregate stability, with an increase in the number of freeze–thaw cycles and accumulated snowfall resulting in an increase in the proportion of aggregates > 0.25 mm, which improved aggregate stability. In addition, the total value of the breakdown path of macro-aggregates increased during the spring freeze–thaw cycling period. Soil moisture was significantly negatively correlated with aggregate stability, with increased soil moisture resulting in a decrease in the percentage of aggregates > 0.25 mm, which resulted in a decrease in aggregate stability. The study can provide a reference understanding for the effects of freeze–thaw cycles on the structure of black soil and provide a theoretical basis for improving the quality of arable land.

Lunar lava tube caves, due to their unique geographical structure and potential as shelters, have repeatedly emerged as prime candidates or priority areas during discussions on lunar base site selection. However, owing to current technological limitations and the complexities of lunar exploration, obtaining detailed data directly from the interiors of lunar lava tubes poses a significant challenge. Hence, the study of terrestrial lava tubes assumes critical importance. By delving into Earth’s lava tubes, we can gain insights into crucial aspects such as their formation mechanisms, geological characteristics, and stability, which can then inform and guide research on lava tubes found on extraterrestrial bodies like the Moon and Mars. During the data acquisition process for lava tubes, given their intricate spatial configurations and dim lighting conditions, we have opted to employ LiDAR technology to collect point cloud data, revealing the internal structural information of the lava tubes. Consequently, this paper thoroughly explores a method for extracting lava tube parameters and constructing models using this point cloud data. This approach encompasses four key steps: data preprocessing, axis construction of the lava tube, cross-sectional feature extraction, and 3D model reconstruction. Initially, raw data is refined through the creation of point cloud voxels to mitigate noise interference. Subsequently, an iterative algorithm is utilized to obtain the L1-medial skeleton, enabling precise capture of the lava tube’s axis information. Using this extracted axis, we proceed with sampling at fixed intervals to derive cross-sectional points of the lava tube. These points are then utilized to extract fundamental geometric parameters such as cross-sectional area, length, and width. Ultimately, by integrating all these cross-sectional point cloud data, a comprehensive 3D model of the lava tube is constructed. Remarkably, this method achieves a high degree of accuracy, with 99 % of the extracted cross-sectional points exhibiting an error of less than 0.12 m, and 99 % of the model’s positional errors falling within 0.15 m. This not only enhances the precision of parameter extraction but also provides significant technical support for in-depth studies of extraterrestrial volcanic activities and lava tube formation mechanisms. Furthermore, it offers a valuable tool for parameter extraction and research on other irregular natural tunnels.

The tensile and fatigue properties of a newly developed Ni-based superalloy, HAYNES® 233, were investigated. Two different manufacturing processes including hot rolling (wrought plate) and laser-powder directed energy deposition (LP-DED) were considered. In addition, specimens were tested in two heat treatment (HT) conditions, namely solution annealed (SA), and age-hardened (AH). The microstructures of the materials were characterized using a scanning electron microscope. Tensile properties of specimens were evaluated at 21 °C, 538 °C, and 871 °C along with the fatigue properties at 21 °C under two different strain amplitudes of εa = 0.005 mm/mm and εa = 0.002 mm/mm. The LP-DED material in both the SA and AH conditions showed similar tensile strengths to the wrought AH material at all test temperatures, whereas the wrought SA material showed a noticeable deficit in strength at 21 °C and 538 °C, and was only comparable at 871 °C. Fatigue results indicated that there was no significant difference in fatigue life for specimens tested at εa = 0.005 mm/mm, while differences in fatigue life were observed for specimens tested at εa = 0.002 mm/mm, where fatigue life was observed to correlate well with the size of the crystallographic facets at crack initiation sites.

Abstract Respiratory syncytial virus (RSV) is one of the most important pathogens causing respiratory tract infection in humans, especially in infants and the elderly. The identification and structural resolution of the potent neutralizing epitopes on RSV fusion (F) protein enable an “epitope-focused” vaccine design. However, the display of RSV F epitope II on the surface of the widely-used human hepatitis B virus core antigen (HBcAg) has failed to induce neutralizing antibody response in mice. Here, we used the hepadnavirus core protein (HcAg) from different mammalian hosts as scaffolds to construct chimeric virus-like particles (VLPs) presenting the RSV F epitope II. Mouse immunization showed that different HcAg-based chimeric VLPs elicited significantly different neutralizing antibody responses, among which the HcAg derived from roundleaf bat (RBHcAg) is the most immunogenic. Furthermore, RBHcAg was used as the scaffold platform to present multiple RSV F epitopes, and the immunogenicity was further improved in comparison to that displaying a single epitope II. The designed RBHcAg-based multiple-epitope-presenting VLP formulated with MF59-like adjuvant elicited a potent and balanced Th1/Th2 immune response, and offered substantial protection in mice against the challenge of live RSV A2 virus. The designed chimeric VLPs may serve as the potential starting point for developing epitope-focused vaccines against RSV. Our study also demonstrated that RBHcAg is an effective VLP carrier for presenting foreign epitopes, providing a promising platform for epitope-focused vaccine design.

Abstract This study aims to delineate landslide susceptibility maps using the Analytical Hierarchy Process (AHP) method for the Great Xi’an Region, China, which is a key planning project for urban construction in Shaanxi Province, China from 2021 to 2035. Multiple data as elevation, slope, aspect, curvature, river density, soil, lithology, and land use have been considered for delineating the landslide susceptibility maps. Spatially thematic layers and distributed maps of all the aforementioned parameters were created in a GIS environment. Determine the relative importance of these thematic layers in the occurrence of landslides in the study area concerning historical landslide data to assign appropriate weights. Landslide sensitivity maps were generated by a weighted combination in a GIS environment after being analyzed by the AHP method. The sensitivity maps were categorized as “very high (11.06%), high (19.41%), moderate (23.03%), low (28.70%), and very low (17.80%)”. Overlay analysis of the test data with the LSM showed that the moderate to very high landslide susceptibility zones were able to contain 82.58% of the historic landslides. The results of the study help determine the landslide-prone areas in the area and provide a reference for subsequent construction. In addition, the analysis of landslide susceptibility in the area contributes to the study of landslides in similar loess sites.

Abstract Modulation of cell death is a powerful strategy employed by pathogenic bacteria to evade host immune clearance and occupy profitable replication niches during infection. Intracellular pathogens employ the type III secretion system (T3SS) to deliver effectors, which interfere with regulated cell death pathways to evade immune defenses. Here, we reveal that poly(ADP-ribose) polymerase-1 (PARP1)-dependent cell death restrains Edwardsiella piscicida’s proliferation in mouse monocyte macrophages J774A.1, of which PARP1 activation results in the accumulation of poly(ADP-ribose) (PAR) and enhanced inflammatory response. Moreover, E. piscicida, an important intracellular pathogen, leverages a T3SS effector YfiD to impair PARP1’s activity and inhibit PAR accumulation. Once translocated into the host nucleus, YfiD binds to the ADP-ribosyl transferase (ART) domain of PARP1 to suppress its PARylation ability as the pharmacological inhibitor of PARP1 behaves. Furthermore, the interaction between YfiD and ART mainly relies on the complete unfolding of the helical domain, which releases the inhibitory effect on ART. In addition, YfiD impairs the inflammatory response and cell death in macrophages and promotes in vivo colonization and virulence of E. piscicida. Collectively, our results establish the functional mechanism of YfiD as a potential PARP1 inhibitor and provide more insights into host defense against bacterial infection.

Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer and poses a significant public health challenge. Early detection is crucial for improving patient outcomes, with serum biomarkers such as carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCCAg), and cytokeratin fragment 19 (CYFRA 21-1) playing a critical role in early screening and pathological classification of NSCLC. However, due to being mainly based on corresponding antibody binding reactions, existing detection technologies for these serum biomarkers have shortcomings such as complex operations, high false positive rates, and high costs. This study aimed to develop new methods for detecting CEA, SCCAg, and CYFRA 21-1 to assist in the diagnosis of NSCLC. Affinity peptides of CEA, SCCAg, and CYFRA 21-1, respectively, were screened by phage display technology, and the peptides’ binding affinities were determined by enzyme-linked immunosorbent assay and biolayer interferometry. Peptides with high affinity were then integrated as binding domains into biosensors by fusing them with circularly permuted fluorescent proteins (cpFPs) through genetic coding. The resulting biosensors, C4 biosensor for CEA, S1 biosensor for SCCAg, and Y3 biosensor for CYFRA 21-1, demonstrated robust sensitivity and specificity even at concentrations as low as 1 ng/mL for their respective tumor markers. When applied to clinical samples and recalibrated for the upper limit of normal concentrations, the biosensors exhibited enhanced sensitivity and specificity for NSCLC diagnosis. This study introduced innovative biosensors for the detection of CEA, SCCAg, and CYFRA 21-1, providing a highly sensitive, specific, rapid, and cost-effective diagnostic alternative that could significantly improve NSCLC screening rates.

Infrared thermography (IRT) is an effective nondestructive testing method for detecting delamination in concrete structures. However, erroneous data interpretation often diminishes its practical utility due to surface irregularities (e.g., color variations) during inspection. These “noisy conditions” alter the temperature distribution of the structure under solar heating cycles, making it challenging to quantify delamination based on the developed thermal contrast (ΔT). This study experimentally investigates the impact of different surface conditions (bare concrete vs. painted surfaces) on ΔT. Artificial delamination at varying depths was simulated and tested under natural environmental conditions, where the maximum ΔT values for shallow delamination were 9.40 °C (bare concrete), 7.35 °C (yellow paint), and 5.15 °C (white paint), respectively. This study measured and analyzed the absorptivity (bare concrete: 0.652, yellow paint: 0.538, and white paint: 0.369), emissivity, and the temperature difference (δT) between non-delaminated areas and air, revealing their correlation with ΔT variation. Based on the results, three typical scenarios are proposed to correlate δT with delamination detection. These findings contribute to a better understanding of the optimal detection window and present a new approach to quantifying delamination at different time windows. The conclusion also discusses the limitations of this study and future research directions.

Bovine respiratory syncytial virus (BRSV) is an enveloped RNA virus that utilizes clathrin-mediated endocytosis for cell entry and is a significant pathogen in bovine respiratory disease (BRD). Heat shock protein family A member 4 (HSPA4), a member of the HSP70 family, is known to be involved in the progression of various cancers. However, its role in virus entry has not been previously explored. Through experiments involving Western blot analysis, virus titer, and virus copies analysis, we demonstrated that HSPA4 can regulate BRSV entry and replication. The specific regulation mode is to enhance BRSV entry by promoting clathrin-mediated endocytosis. We used Western blot, virus titer, virus copies analysis, and IFA to demonstrate that HSPA4 can promote clathrin heavy chain protein (CHC) expression and further promote BRSV entry by activating the PI3K–Akt signaling pathway. Furthermore, we observed that HSPA4 boosts the efficiency of clathrin-mediated endocytosis by increasing the ATPase activity of heat shock cognate protein 70 (HSC70), thereby facilitating BRSV entry. Additionally, our investigation into the impact of HSPA4 on the entry of other viruses revealed that HSPA4 can facilitate the entry of a variety of viruses into host cells.

Abstract Long non‐coding RNA RP11‐64B16.4 (myocardial infarction protection‐related lncRNA [MIPRL]) is among the most abundant and the most upregulated lncRNAs in ischemic human hearts. However, its role in ischemic heart disease is unknown. We found MIPRL was conserved between human and mouse and its expression was increased in mouse hearts after acute myocardial infarction (AMI) and in cultured human and mouse cardiomyocytes after hypoxia. The infarcted size, cardiac cell apoptosis, cardiac dysfunction, and cardiac fibrosis were aggravated in MIPRL knockout mice after AMI. The above adverse results could be reversed by re‐expression of MIPRL via adenovirus expressing MIPRL. Both in vitro and in vivo, we identified that heat shock protein beta‐8 (HSPB8) was a target gene of MIPRL, which was involved in MIPRL‐mediated anti‐apoptotic effects on cardiomyocytes. We further discovered that MIPRL could combine with the messenger RNA (mRNA) of HSPB8 and increase its expression in cardiomyocytes by enhancing the stability of HSPB8 mRNA. In summary, we have found for the first time that the ischemia‐enhanced lncRNA MIPRL protects against AMI via its target gene HSPB8. MIPRL might be a novel promising therapeutic target for ischemic heart diseases such as AMI.

Legionella pneumophila strains harboring wild-type rpsL such as Lp02rpsLWT cannot replicate in mouse bone marrow-derived macrophages (BMDMs) due to induction of extensive lysosome damage and apoptosis. The bacterial factor directly responsible for inducing such cell death and the host factor involved in initiating the signaling cascade that leads to lysosome damage remain unknown. Similarly, host factors that may alleviate cell death induced by these bacterial strains have not yet been investigated. Using a genome-wide CRISPR/Cas9 screening, we identified Hmg20a and Nol9 as host factors important for restricting strain Lp02rpsLWT in BMDMs. Depletion of Hmg20a protects macrophages from infection-induced lysosomal damage and apoptosis, allowing productive bacterial replication. The restriction imposed by Hmg20a was mediated by repressing the expression of several endo-lysosomal proteins, including the small GTPase Rab7. We found that SUMOylated Rab7 is recruited to the bacterial phagosome via SulF, a Dot/Icm effector that harbors a SUMO-interacting motif (SIM). Moreover, overexpression of Rab7 rescues intracellular growth of strain Lp02rpsLWT in BMDMs. Our results establish that L. pneumophila exploits the lysosomal network for the biogenesis of its phagosome in BMDMs.

Abstract Introduction The susceptibility to surgical site occurrence (SSO) is high following ventral hernia repair (VHR) surgery. SSO severely increases the physical and mental burden on patients. The main purpose of this review was to analyze the efficacy of negative pressure wound therapy (NPWT) after open VHR(OVHR) and explore benefits to patients. Methods The Cochrane Library, PubMed, and Embase databases were searched from the date of establishment to 15 October 2022. All randomized controlled trials and retrospective cohort studies comparing NPWT with standard dressings after OVHR were included. The Revman 5.4 software recommended by Cochrane and the STATA16 software were used in this meta-analysis. Results Fifteen studies (involving 1666 patients) were identified and included in the meta-analysis, with 821 patients receiving NPWT. Overall, the incidence rate of SSO in the NPWT group was lower compared to the control group (odds ratio [OR] = 0.44; 95% confidence interval [CI] = 0.21–0.93; I2 = 86%; P = 0.03). The occurrence rate of surgical site infection (SSI; OR = 0.51; 95% CI = 0.38–0.68, P

The central environmental protection inspection (CEPI) system in China is a significant institutional innovation in national environmental governance. The CEPI applies a joint supervision strategy to address salient environmental issues and strictly enforce the environmental responsibilities of local governments. This study collects and organizes CEPI inspection reports covering three stages that encompass the first round, the “look back”, and the second round, applying text analysis to obtain sample data and conduct statistical quantification of word frequency in inspection reports and identify notable changes. The study explores the allocation of CEPI attention between policy objectives and the intensity of policy instruments. We determine that in conjunction with public opinion feedback, the CEPI conducts targeted inspections and focuses more on pollutant governance, which has high severity and can be addressed quickly. The CEPI fills the gap of normalized governance with a campaign-style governance approach. Regarding the intensity of policy measures, the CEPI primarily uses economic incentive policy instruments, supplemented by command-and-control and public guidance approaches, advancing the sustainability of regulatory effectiveness through economic, social, and political activities. This study extends knowledge in the field of CEPI policy priorities and implementation, expanding the literature related to outcomes of environmental policy in developing countries.

For rapid and convenient detection of living endothelial cells (ECs) specifically without immunostaining, we developed a biosensor based on turn-on fluorescent protein, named LV-EcpG. It includes a high-affinity peptide E12P obtained through phage display technology for specifically recognizing ECs and a turn-on EGFP fused with two linker peptides. The “on-off” switching mechanism of this genetically encoded fluorescent protein-based biosensor (FPB) ensured that fluorescence signals were activated only when binding with ECs, thus enabling these FPB characters for direct, visual, and non-invasive detection of ECs. Its specificity and multicolor imaging capability established LV-EcpG as a powerful tool for live EC research, with significant potential for diagnosing and treating cardiovascular diseases and tumor angiogenesis.

In order to clarify the effect of mixed lubrication methods on the oil flow and power loss of the gearbox, this study adopts a high-precision moving particle semi-implicit (MPS) method to investigate the lubrication of the gearbox under the joint influence of splash lubrication and oil injection lubrication. The accuracy of the numerical method to calculate the churning torque was verified by the constructed test rig. The effects of rotational speed, immersion depth, injection volume rate, and oil injection angle were analyzed and evaluated for lubrication. The results show that better lubrication can be achieved with relatively small churning torques by using a hybrid lubrication method. This provides some references for engineering applications of gearboxes.

Monocytes are key effectors in autoimmunity-related diseases in the central nervous system (CNS) due to the critical roles of these cells in the production of proinflammatory cytokines, differentiation of T-helper (Th) cells, and antigen presentation. The JAK–STAT signaling is crucial for initiating monocytes induced immune responses by relaying cytokines signaling. However, the role of this pathway in modulating the communication between monocytes and Th cells in the pathogenesis of multiple sclerosis (MS) is unclear. Here, we show that the JAK1/2/3 and STAT1/3/5/6 subtypes involved in the demyelination mediated by the differentiation of pathological Th1 and Th17 and the CNS-infiltrating inflammatory monocytes in experimental autoimmune encephalomyelitis (EAE), a model for MS. JAK inhibition prevented the CNS-infiltrating CCR2-dependent Ly6Chi monocytes and monocyte-derived dendritic cells in EAE mice. In parallel, the proportion of GM-CSF+CD4+ T cells and GM-CSF secretion were decreased in pathological Th17 cells by JAK inhibition, which in turns converted CNS-invading monocytes into antigen-presenting cells to mediate tissue damage. Together, our data highlight the therapeutic potential of JAK inhibition in treating EAE by blocking the GM-CSF-driven inflammatory signature of monocytes.

Abstract Background Hepatic fibrosis is a common consequence of chronic liver diseases without approved antifibrotic therapies. Long noncoding RNAs (lncRNAs) play an important role in various pathophysiological processes. However, the functions of certain lncRNAs involved in mediating the antifibrotic role remain largely unclear. Methods The RNA level of lnc-High Expressed in Liver Fibrosis (Helf) was detected in both mouse and human fibrotic livers. Furthermore, lnc-Helf-silenced mice were treated with carbon tetrachloride (CCl4) or bile duct ligation (BDL) to investigate the function of lnc-Helf in liver fibrosis. Results We found that lnc-Helf has significantly higher expression in human and mouse fibrotic livers as well as M1 polarized hepatic macrophages (HMs) and activated hepatic stellate cells (HSCs). In vivo studies showed that silencing lnc-Helf by AAV8 vector alleviates CCl4- and BDL-induced hepatic inflammation and fibrosis. Furthermore, in vitro experiments revealed that lnc-Helf promotes HSCs activation and proliferation, as well as HMs M1 polarization and proliferation in the absence or presence of cytokine stimulation. Mechanistically, our data illustrated that lnc-Helf interacts with RNA binding protein PTBP1 to promote its interaction with PIK3R5 mRNA, resulting in increased stability and activating the AKT pathway, thus promoting HSCs and HMs activation and proliferation, which augments hepatic inflammation and fibrosis. Conclusion Our results unveil a lnc-Helf/PTBP1/PIK3R5/AKT feedforward, amplifying signaling that exacerbates the process of hepatic inflammation and fibrosis, thus providing a possible therapeutic strategy for hepatic fibrosis.

Abstract Neutrophils have a pathogenic function in inflammation via releasing pro-inflammatory mediators or neutrophil extracellular traps (NETs). However, their heterogeneity and pro-inflammatory mechanisms remain unclear. Here, we demonstrate that CXCR4hi neutrophils accumulate in the blood and inflamed skin in human psoriasis, and correlate with disease severity. Compared to CXCR4lo neutrophils, CXCR4hi neutrophils have enhanced NETs formation, phagocytic function, neutrophil degranulation, and overexpression of pro-inflammatory cytokines and chemokines in vitro. This is accompanied by a metabolic shift in CXCR4hi neutrophils toward glycolysis and lactate release, thereby promoting vascular permeability and remodeling. CXCR4 expression in neutrophils is dependent on CREB1, a transcription factor activated by TNF and CXCL12, and regulated by de novo synthesis. In vivo, CXCR4hi neutrophil infiltration amplifies skin inflammation, whereas blockade of CXCR4hi neutrophils through CXCR4 or CXCL12 inhibition leads to suppression of immune responses. In this work, our study identifies CREB1 as a critical regulator of CXCR4hi neutrophil development and characterizes the contribution of CXCR4hi neutrophils to vascular remodeling and inflammatory responses in skin.

Helminths produce calreticulin (CRT) to immunomodulate the host immune system as a survival strategy. However, the structure of helminth-derived CRT and the structural basis of the immune evasion process remains unclarified. Previous study found that the tissue-dwelling helminth Trichinella spiralis produces calreticulin (TsCRT), which binds C1q to inhibit activation of the complement classical pathway. Here, we used x-ray crystallography to resolve the structure of truncated TsCRT (TsCRTΔ), the first structure of helminth-derived CRT. TsCRTΔ was observed to share the same binding region on C1q with IgG based on the structure and molecular docking, which explains the inhibitory effect of TsCRT on C1q-IgG–initiated classical complement activation. Based on the key residues in TsCRTΔ involved in the binding activity to C1q, a 24 amino acid peptide called PTsCRT was constructed that displayed strong C1q-binding activity and inhibited C1q-IgG–initiated classical complement activation. This study is the first to elucidate the structural basis of the role of TsCRT in immune evasion, providing an approach to develop helminth-derived bifunctional peptides as vaccine target to prevent parasite infections or as a therapeutic agent to treat complement-related autoimmune diseases.

BackgroundSilicosis shows an increasing trend with the development of new industries. However, the potential biomarkers for predicting the disease severity are lacking. A novel inflammatory marker, the systemic immune-inflammation Index (SII), has not been studied in silicosis.MethodsIn this retrospective study, we used data from a big database platform of a tertiary general hospital in Beijing, which was established based on the electronic medical records of the hospital. The clinical data of adult patients diagnosed with silicosis at the Department of Occupational Medicine and Toxicology from 2013 to 2022 were collected. The data extracted from the database were in de-identified form. Only patients with a first diagnosis of silicosis and without conditions that might affect the parameters of routine blood tests were included in the analysis. Analyses were performed to assess the relationship between SII and the advanced stage of silicosis.ResultsA total of 246 participants were included in the study. Most of the patients were exposed to silica particles during excavation and digging (n = 149, 60.6%). SII level was significantly higher in patients with advanced stages of silicosis. A multivariate logistic regression analysis revealed that a higher SII level was associated with the advanced stage of silicosis [odds ratio (OR) = 1.002; 95% confidence interval (CI): 1.000–1.003, p

In this work, a hybrid integrated tunable external cavity laser (ECL) with a sub-10 Hz intrinsic linewidth is demonstrated. In this hybrid ECL, two Si3N4-based subwavelength hole defect assisted microring resonators, acting as high-Q laser reflectors, are butt-coupled with a chip-scale semiconductor optical amplifier at the two edges, respectively. The experimental results show that the hybrid ECL achieves a sub-10 Hz intrinsic linewidth across a wavelength tuning range from 1525 to 1565 nm, featuring a record lowest value of 6.06 Hz. The demonstrated hybrid ECL opens up opportunities toward the applications of high-speed optical communications and high-resolution light detection and ranging.

Abstract Semen Ziziphi Spinosae protein (SZSP) is a new plant protein resource with good food functional properties and health care function. However, the biological activity of SZSP has not been further studied, which greatly limits the development and utilization of SZSP in the food industry. The aim of this study was to investigate the protective effect of SZSP on immunosuppressed mice and its inhibitory effect on immune-stimulated RAW264.7 cells. The results demonstrated that SZSP remarkably improved the immunomodulatory secretion in serum (interleukin-2, tumor necrosis factor-α [TNF-α], interferon-γ, immunoglobulin-A, immunoglobulin-G, immunoglobulin-M) and primary macrophages (nitric oxide, interleukin-1β, TNF-α) and promoted the NK-cell killing activity of primary splenocytes in CTX-induced immunosuppression mice. Immunohistochemical analysis results indicated that the secretion of CD4 + and CD8 + in the spleen and thymus can be regulated by SZSP, leading to inhibition of the damage induced by cyclophosphamide in mice. Meanwhile, in order to clarify the immunomodulatory mechanism of SZSP, we showed that SZSP significantly inhibited the secretion of NO, interleukin-6, and TNF-α and reduced the phosphorylation expression of p-ERK, p-JNK, and p-IκBα in lipopolysaccharide-stimulated RAW264.7 cells. Therefore, the immunomodulatory effect of SZSP may be related to the activation of MAPKs and NF-κB signaling pathways. Based on the above studies, the preliminary purification of SZSP was continued, and S1F2G1 with immunomodulatory activity was obtained. Taken together, SZSP has an immunoregulatory effect in vivo and in vitro and may be a favorable candidate of functional food raw material for regulating immune responses.

Background: The TGF-β gene is a gemcitabine (GEM) resistance gene; however, the mechanism by which it regulates GEM resistance in pancreatic cancer remains unclear. Methods: The PANC-1 cell line was treated with GEM and then stimulated with TGF-β. Subsequently, we constructed GEM-resistant pancreatic cancer cell lines, knocked down TGF-β in these cell lines, and detected changes in the proliferation and apoptosis of drug-resistant cancer cells. In addition, the protein expression levels of KLF-4, GFI-1, and ZEB-1 were determined. The xenograft tumor models of nude mice were constructed by subcutaneously injecting GEM-resistant PANC-1 cells into mouse axilla. The tumors were removed, dissected, and weighed after 6 weeks. The protein levels of KLF-4, GFI-1, and ZEB-1 in tumor tissues were quantified. In addition, the percentage of M2 macrophages in tumor tissues was determined using flow cytometry. Results: The protein levels of TGF-β in pancreatic cancer cells were significantly decreased after GEM treatment. The protein expression of KLF-4 was downregulated, whereas the expressions of GFI-1 and ZEB-1 were upregulated after TGF-β stimulation. Apoptosis increased and proliferation decreased after TGF-β knockdown in GEM-resistant pancreatic cancer cells, moreover, silencing TGF-β promoted the expression of Caspase 3 and Cleaved caspase 3. In addition, the protein expression of KLF-4 was upregulated, whereas the expressions of GFI-1 and ZEB-1 were downregulated. Further, the volume and weight of the transplanted tumor decreased after TGF-β knockdown. The protein expression of KLF-4 was upregulated, whereas the expressions of GFI-1 and ZEB-1 were downregulated in tumor tissues. In addition, the percentage of M2 macrophages decreased in tumor tissues after TGF-β knockdown. Conclusions: The knockdown of TGF-β inhibits epithelial-to-mesenchymal transition, suppresses the proliferation and promotes the apoptosis of drug-resistant cancer cells, and decreases the macrophage polarization to the M2 phenotype, consequently ameliorating GEM resistance in pancreatic cancer.

Far-red fluorescent proteins (FPs) have emerged as indispensable tools in in vivo imaging, playing a pivotal role in elucidating fundamental mechanisms and addressing application issues in biotechnology and biomedical fields. Their ability for deep penetration, coupled with reduced light scattering and absorption, robust resistance to autofluorescence, and diminished phototoxicity, has positioned far-red biosensors at the forefront of non-invasive visualization techniques for observing intracellular activities and intercellular behaviors. In this review, far-red FPs and their applications in living systems are mainly discussed. Firstly, various far-red FPs, characterized by emission peaks spanning from 600 nm to 650 nm, are introduced. This is followed by a detailed presentation of the fundamental principles enabling far-red biosensors to detect biomolecules and environmental changes. Furthermore, the review accentuates the superiority of far-red FPs in multi-color imaging. In addition, significant emphasis is placed on the value of far-red FPs in improving imaging resolution, highlighting their great contribution to the advancement of in vivo imaging.

To investigate the lubrication characteristics in high-speed train gearboxes, a two-stage herringbone gearbox with an idle gear was analyzed. The lubricant flow and distribution were shown using the moving particle semi-implicit (MPS) method. A liquid film flow model was brought in to enhance the non-slip wall boundary conditions, enabling MPS to predict the film flow characteristics. This study investigates the influence of gear rotating speed, lubricant volume, and temperature on lubricant flow, liquid film distribution, lubrication state in the meshing zone, and churning power loss. The results indicate that lubrication characteristics depend on the splashing effect of rotating gears and lubricant fluidity. Increasing gear rotating speed and lubricant temperature can improve liquid film distribution on the inner wall, increase lubricant volume, and thus enhance film thickness. The lubricant particles in the meshing zone correlate positively with the gear rotating speed and lubricant volume, correlate negatively with a temperature above 20 °C, and decrease notably at low temperatures. Churning power loss mainly comes from the output gear. As lubricant volume and gear rotating speed increase, churning torque and power loss increase. Above 20 °C, viscosity decreases, reducing power loss; low temperatures lessen lubricant fluidity, reducing churning power loss.

Ubiquitination is crucial for the growth of cancer. However, the role of ubiquitination-related genes (URGs) in stomach adenocarcinoma (STAD) remains unclear. Differentially expressed URGs (DE-URGs) were examined in the whole TCGA-STAD dataset, and the prognosis-related genes were discovered from the The Cancer Genome Atlas (TCGA) training set. Prognostic genes were discovered using selection operator regression analysis and absolute least shrinkage (LASSO). A multivariate Cox analysis was further employed, and a polygene-based risk assessment system was established. Signatures were verified using the Gene Expression Omnibus (GEO) database record GSE84433 and the TCGA test set. Using the MEXPRESS dataset, a detailed analysis of gene expression and methylation was carried out. Using the DAVID database, DE-URG function and pathway enrichment was examined. The identified 163 DE-URGs were significantly associated with pathways related to protein ubiquitination, cell cycle, and cancer. A prognostic signature based on 13 DE-URGs was constructed, classifying patients into two risk groups. Compared to low-risk patients, people at high risk had considerably shorter survival times. Cox regression analyses considered prognostic parameters independent of age and risk score and were used to generate nomograms. Calibration curves show good agreement between nomogram predictions and observations. Furthermore, the results of the MEXPRESS analysis indicated that 13 prognostic DE-URGs had an intricate methylation profile. The enhanced Random Forest-based model showed greater efficacy in predicting prognosis, mutation, and immune infiltration. The in vitro validation, including CCK8, EdU, Transwell, and co-culture Transwell, proved that RNF144A was a potent oncogene in STAD and could facilitate the migration of M2 macrophages. In this research, we have created a genetic model based on URGs that can appropriately gauge a patient’s prognosis and immunotherapy response, providing clinicians with a reliable tool for prognostic assessment and supporting clinical treatment decisions.

The reliability of additively manufactured flexible electronics or so-called printed electronics is defined as mean time to failure under service conditions, which often involve mechanical loads. It is thus important to understand the mechanical behavior of the printed materials under such conditions to ensure their applicational reliability in, for example, sensors, biomedical devices, battery and storage, and flexible hybrid electronics. In this article, a testing protocol to examine the print quality of additively nanomanufactured electronics is presented. The print quality is assessed by both tensile and electrical resistivity responses during in-situ tension tests. A laser based additive nanomanufacturing method is used to print conductive silver lines on polyimide substrates, which is then tested in-situ under tension inside a scanning electron microscope (SEM). The surface morphology of the printed lines is continuously monitored via the SEM until failure. In addition, the real-time electrical resistance variations of the printed silver lines are measured in-situ with a multimeter during tensile tests conducted outside of the SEM. The protocol is shown to be effective in assessing print quality and aiding process tuning. Finally, it is revealed that samples appearing identical under the SEM can have significant different tendencies to delaminate.

In the current study, the effects of different post-processing methods, including heat treatment (HT) and electro-chemical polishing (ECP) as well as their combination on the surface texture, porosity, microstructure, mechanical properties, and rotating bending fatigue behavior of U-notched laser powder bed fused AlSi10Mg specimens were comprehensively investigated. In addition, to better understand the effects of the applied post-processing methods on the sensitivity of the notched specimen to surface and near-surface defects, finite element analysis was performed. Chemical treatment was found to be very influential on surface texture modification of the very narrow notched parts, for which the application of other treatments can be quite challenging. It was also found that the fatigue behavior of the notched specimens was more sensitive to the surface texture rather than to the near-surface defects. The hybrid treatment involving HT+ECP was the most effective for fatigue behavior improvement due to simultaneous homogenization of the microstructure, released tensile residual stresses, enhanced ductility and high surface texture modification.

In order to protect natural ecosystems, areas that can provide higher ecosystem services are usually designated as ecological priority protection areas. However, some areas with good natural and socio-cultural conditions (called ecological improvement zones in this study, EIZs) that do not provide the highest ecosystem services are often ignored. Therefore, this study attempts to use ecological functional zones as the research unit. And delineating the EIZs by combining multiple critical impact thresholds of ecosystem health (EH) change. Northwestern Hubei Province (NWHB), an important ecological function area in China, was selected as the study area. The results show that (1) the landscape of cultivated land tends to be fragmented, due to being occupied by a large amount of rural–urban land; (2) from 2000 to 2020, the EH in NWHB gradually improved; (3) spatially, the western region of NWHB is dominated by multiple ESs synergetic zone, while the eastern region is a food provision zone; and (4) except for GDP and population density, which are monotonically negatively correlated with EH, all other natural and anthropogenic factors have critical impact thresholds. The critical impact thresholds vary significantly among different ecological functional zones in different periods (e.g., In biodiversity - carbon storage synergetic zone, the threshold of DEM of around less than 1150 m in 2000 decreased to 480 m in 2005 with a clear trend of EH improvement). Meanwhile, from 2000 to 2020, EIZs are mainly distributed in key synergistic bundles in different periods. This study aims to provide services for the planning and management of ecological functional zones.

Abstract Hydrogen, oxygen, carbon, and nitrogen isotopes derived from three different strains of silkworms at different life stages involved in silkworm rearing, were measured to understand the fractionation characteristics of stable isotopes at different stages of silkworm development, and to trace the movement of these isotopes from food to larva to excrement and finally to silk. We found that silkworm strain had little effect on δ2H, δ18O and δ13C values. However, a large difference was found in the δ15N levels of newly-hatched silkworms between Jingsong Haoyue and Hua Kang No. 3 orthogonal strains, suggesting that the mating and egg laying differences may result in an inconsistent kinetic nitrogen isotope fractionation. The δ13C values of silkworm pupae and silkworm cocoon also displayed significant differences, suggesting that heavy carbon isotopes are greatly fractionated from the larva to the silk during cocoon formation. Overall, these results may be used to clarify the relationship between isotope fractionation and the ecological process of the Bombyx mori and expand our ability to resolve stable isotope anomalies at a small regional-scale level.

Abstract An ongoing randomized, double-blind, controlled phase 2 trial was conducted to evaluate the safety and immunogenicity of a mosaic-type recombinant vaccine candidate, named NVSI-06-09, as a booster dose in subjects aged 18 years and older from the United Arab Emirates (UAE), who had administered two or three doses of inactivated vaccine BBIBP-CorV at least 6 months prior to enrollment. The participants were randomly assigned with 1:1 to receive a booster dose of NVSI-06-09 or BBIBP-CorV. The primary outcomes were immunogenicity and safety against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant, and the exploratory outcome was cross-immunogenicity against other circulating strains. Between May 25 and 30, 2022, 516 adults received booster vaccination with 260 in NVSI-06-09 group and 256 in BBIBP-CorV group. Interim results showed a similar safety profile between two booster groups, with low incidence of adverse reactions of grade 1 or 2. For immunogenicity, by day 14 post-booster, the fold rises in neutralizing antibody geometric mean titers (GMTs) from baseline elicited by NVSI-06-09 were remarkably higher than those by BBIBP-CorV against the prototype strain (19.67 vs 4.47-fold), Omicron BA.1.1 (42.35 vs 3.78-fold), BA.2 (25.09 vs 2.91-fold), BA.4 (22.42 vs 2.69-fold), and BA.5 variants (27.06 vs 4.73-fold). Similarly, the neutralizing GMTs boosted by NVSI-06-09 against Beta and Delta variants were also 6.60-fold and 7.17-fold higher than those by BBIBP-CorV. Our findings indicated that a booster dose of NVSI-06-09 was well-tolerated and elicited broad-spectrum neutralizing responses against divergent SARS-CoV-2 variants, including Omicron and its sub-lineages.

Data-driven ship detection methods via deep learning algorithms are the recent research hotspot. In this family of models, it is essential to divide the positive and the negative samples. The commonly used strategy is called label assignment. In the previous detectors, label assignment resulted from the handcrafted heuristic techniques. Therefore, it needs to tune the hyperparameters, and improper settings will deteriorate model performance. Moreover, a significant inconsistency between the training and the testing objective is available. To address these issues, a prediction and prior information guided label assignment technique is proposed. A network specific to ship detection is then presented to improve multiscale detection performance. First, the IoU prediction is specified as the estimation of localization precision. The quality of the candidate anchor is evaluated by a combination of classification and positioning. It reduces the inconsistency between training and testing. Besides, the learning status of the current model and the anchors' prior information are exploited simultaneously. Optimum positive samples are selected in an adaptive manner. Finally, a multiscale ship detection network is designed, concentrating on small ships' rich contextual information. After feature fusion and feature enhancement on different scales, shallow texture and deep semantic information are combined to detect multiscale ships. Multiple experiments are conducted on SSDD and HRSID datasets, and the results demonstrate the advantage of the proposed method compared with advanced detectors.